LOCUS       sequence-501697-       10201 bp    DNA     circular SYN 04-MAR-2026
DEFINITION  In vitro transcription of Cas9-BARD1 TruEditor for mRNA generation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10201)
  AUTHORS   Burrell, Simon J. Mueller, Zharko Daniloski, P. Duffy Doyle Jr., 
            Anne B. Rovsing, Christopher James, Max Drabkin, Chien-Yu Chou, Hei 
            Yu Annika So, Lyla Katgara, Akash Sookdeo, Lu Lu, Georges-Ibrahim 
            Cisse, Rachel E Yan, Neville E Sanjana
  TITLE     Rational design of synthetic proteins using a genome-scale CRISPR 
            screen
  JOURNAL   bioRxiv
REFERENCE   2  (bases 1 to 10201)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 4, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv"
COMMENT     Sequence Label: pTE_20 -
            pIVT_CMV-synUTR-inactT7-NLS-Cas9-NLS-...
FEATURES             Location/Qualifiers
     source          1..10201
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        54..433
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        434..637
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     588..608
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             740..787
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             788..4888
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             788..4888
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4889..4936
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4937..4960
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             7400..7420
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7430..7456
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     polyA_signal    7564..7788
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7859..7875)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7859..7875)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7872..7894)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7883..7899
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7907..7913,7914..7931,7932..7937))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7952..7973
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8090..8107)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8261..8849)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8341..8360)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9020..9811,9812..9880))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9643..9662
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9881..9985)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10064..10083)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg
       61 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
      121 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
      181 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
      241 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
      301 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
      361 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
      421 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
      481 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
      541 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
      601 taggcgtgta cggtgggagg tctatataag cagagctggt ttagtgaacc gtcagatctc
      661 gagctcggta cctaatacga cacactataa ggaaataaga gagaaaagaa gagtaagaag
      721 aaatataaga gccaccatga agcgacctgc cgccacaaag aaggctggac aggctaagaa
      781 gaagaaagac aagaagtaca gcatcggcct ggacatcggc accaactctg tgggctgggc
      841 cgtgatcacc gacgagtaca aggtgcccag caagaaattc aaggtgctgg gcaacaccga
      901 ccggcacagc atcaagaaga acctgatcgg agccctgctg ttcgacagcg gcgaaacagc
      961 cgaggccacc cggctgaaga gaaccgccag aagaagatac accagacgga agaaccggat
     1021 ctgctatctg caagagatct tcagcaacga gatggccaag gtggacgaca gcttcttcca
     1081 cagactggaa gagtccttcc tggtggaaga ggataagaag cacgagcggc accccatctt
     1141 cggcaacatc gtggacgagg tggcctacca cgagaagtac cccaccatct accacctgag
     1201 aaagaaactg gtggacagca ccgacaaggc cgacctgcgg ctgatctatc tggccctggc
     1261 ccacatgatc aagttccggg gccacttcct gatcgagggc gacctgaacc ccgacaacag
     1321 cgacgtggac aagctgttca tccagctggt gcagacctac aaccagctgt tcgaggaaaa
     1381 ccccatcaac gccagcggcg tggacgccaa ggccatcctg tctgccagac tgagcaagag
     1441 cagacggctg gaaaatctga tcgcccagct gcccggcgag aagaagaatg gcctgttcgg
     1501 aaacctgatt gccctgagcc tgggcctgac ccccaacttc aagagcaact tcgacctggc
     1561 cgaggatgcc aaactgcagc tgagcaagga cacctacgac gacgacctgg acaacctgct
     1621 ggcccagatc ggcgaccagt acgccgacct gtttctggcc gccaagaacc tgtccgacgc
     1681 catcctgctg agcgacatcc tgagagtgaa caccgagatc accaaggccc ccctgagcgc
     1741 ctctatgatc aagagatacg acgagcacca ccaggacctg accctgctga aagctctcgt
     1801 gcggcagcag ctgcctgaga agtacaaaga gattttcttc gaccagagca agaacggcta
     1861 cgccggctac attgacggcg gagccagcca ggaagagttc tacaagttca tcaagcccat
     1921 cctggaaaag atggacggca ccgaggaact gctcgtgaag ctgaacagag aggacctgct
     1981 gcggaagcag cggaccttcg acaacggcag catcccccac cagatccacc tgggagagct
     2041 gcacgccatt ctgcggcggc aggaagattt ttacccattc ctgaaggaca accgggaaaa
     2101 gatcgagaag atcctgacct tccgcatccc ctactacgtg ggccctctgg ccaggggaaa
     2161 cagcagattc gcctggatga ccagaaagag cgaggaaacc atcaccccct ggaacttcga
     2221 ggaagtggtg gacaagggcg cttccgccca gagcttcatc gagcggatga ccaacttcga
     2281 taagaacctg cccaacgaga aggtgctgcc caagcacagc ctgctgtacg agtacttcac
     2341 cgtgtataac gagctgacca aagtgaaata cgtgaccgag ggaatgagaa agcccgcctt
     2401 cctgagcggc gagcagaaaa aggccatcgt ggacctgctg ttcaagacca accggaaagt
     2461 gaccgtgaag cagctgaaag aggactactt caagaaaatc gagtgcttcg actccgtgga
     2521 aatctccggc gtggaagatc ggttcaacgc ctccctgggc acataccacg atctgctgaa
     2581 aattatcaag gacaaggact tcctggacaa tgaggaaaac gaggacattc tggaagatat
     2641 cgtgctgacc ctgacactgt ttgaggacag agagatgatc gaggaacggc tgaaaaccta
     2701 tgcccacctg ttcgacgaca aagtgatgaa gcagctgaag cggcggagat acaccggctg
     2761 gggcaggctg agccggaagc tgatcaacgg catccgggac aagcagtccg gcaagacaat
     2821 cctggatttc ctgaagtccg acggcttcgc caacagaaac ttcatgcagc tgatccacga
     2881 cgacagcctg acctttaaag aggacatcca gaaagcccag gtgtccggcc agggcgatag
     2941 cctgcacgag cacattgcca atctggccgg cagccccgcc attaagaagg gcatcctgca
     3001 gacagtgaag gtggtggacg agctcgtgaa agtgatgggc cggcacaagc ccgagaacat
     3061 cgtgatcgaa atggccagag agaaccagac cacccagaag ggacagaaga acagccgcga
     3121 gagaatgaag cggatcgaag agggcatcaa agagctgggc agccagatcc tgaaagaaca
     3181 ccccgtggaa aacacccagc tgcagaacga gaagctgtac ctgtactacc tgcagaatgg
     3241 gcgggatatg tacgtggacc aggaactgga catcaaccgg ctgtccgact acgatgtgga
     3301 ccatatcgtg cctcagagct ttctgaagga cgactccatc gacaacaagg tgctgaccag
     3361 aagcgacaag aaccggggca agagcgacaa cgtgccctcc gaagaggtcg tgaagaagat
     3421 gaagaactac tggcggcagc tgctgaacgc caagctgatt acccagagaa agttcgacaa
     3481 tctgaccaag gccgagagag gcggcctgag cgaactggat aaggccggct tcatcaagag
     3541 acagctggtg gaaacccggc agatcacaaa gcacgtggca cagatcctgg actcccggat
     3601 gaacactaag tacgacgaga atgacaagct gatccgggaa gtgaaagtga tcaccctgaa
     3661 gtccaagctg gtgtccgatt tccggaagga tttccagttt tacaaagtgc gcgagatcaa
     3721 caactaccac cacgcccacg acgcctacct gaacgccgtc gtgggaaccg ccctgatcaa
     3781 aaagtaccct aagctggaaa gcgagttcgt gtacggcgac tacaaggtgt acgacgtgcg
     3841 gaagatgatc gccaagagcg agcaggaaat cggcaaggct accgccaagt acttcttcta
     3901 cagcaacatc atgaactttt tcaagaccga gattaccctg gccaacggcg agatccggaa
     3961 gcggcctctg atcgagacaa acggcgaaac cggggagatc gtgtgggata agggccggga
     4021 ttttgccacc gtgcggaaag tgctgagcat gccccaagtg aatatcgtga aaaagaccga
     4081 ggtgcagaca ggcggcttca gcaaagagtc tatcctgccc aagaggaaca gcgataagct
     4141 gatcgccaga aagaaggact gggaccctaa gaagtacggc ggcttcgaca gccccaccgt
     4201 ggcctattct gtgctggtgg tggccaaagt ggaaaagggc aagtccaaga aactgaagag
     4261 tgtgaaagag ctactgggga tcaccatcat ggaaagaagc agcttcgaga agaatcccat
     4321 cgactttctg gaagccaagg gctacaaaga agtgaaaaag gacctgatca tcaagctgcc
     4381 taagtactcc ctgttcgagc tggaaaacgg ccggaagaga atgctggcct ctgccggcga
     4441 actgcagaag ggaaacgaac tggccctgcc ctccaaatat gtgaacttcc tgtacctggc
     4501 cagccactat gagaagctga agggctcccc cgaggataat gagcagaaac agctgtttgt
     4561 ggaacagcac aagcactacc tggacgagat catcgagcag atcagcgagt tctccaagag
     4621 agtgatcctg gccgacgcta atctggacaa agtgctgtcc gcctacaaca agcaccggga
     4681 taagcccatc agagagcagg ccgagaatat catccacctg tttaccctga ccaatctggg
     4741 agcccctgcc gccttcaagt actttgacac caccatcgac cggaagaggt acaccagcac
     4801 caaagaggtg ctggacgcca ccctgatcca ccagagcatc accggcctgt acgagacacg
     4861 gatcgacctg tctcagctgg gaggcgacaa gcgacctgcc gccacaaaga aggctggaca
     4921 ggctaagaag aagaaagatt acaaagacga tgacgataag tctggaggat ctagcggatc
     4981 tggaagcgag acaccaggaa caagcgagtc agcaacacca gagagcagtg gcggcagcag
     5041 ctcgggaggt ggttcgggtg gatctggatc accggataat cggcagccga ggaaccggca
     5101 gccgaggatc cgctccggga acgagcctcg ttccgcgtcc gccatggaac cggatggtcg
     5161 cggtgcctgg gcccacagtc gcgccgcgct cgaccgcctg gagaagctgc tgcgctgctc
     5221 gcgttgtact aacattctga gagagcctgt gtgtttagga ggatgtgagc acatcttctg
     5281 tagtaattgt gtaagtgact gcattggaac tggatgtcca gtgtgttaca ccccggcctg
     5341 gatacaagac ttgaagataa atagacaact ggacagcatg attcaacttt gtagtaagct
     5401 tcgaaatttg ctacatgaca atgagctgtc agatttgaaa gaagataaac ctaggaaaag
     5461 tttgtttaat gatgcaggaa acaagaagaa ttcaattaaa atgtggttta gccctcgaag
     5521 taagaaagtc agatatgttg tgagtaaagc ttcagtgcaa acccagcctg caataaaaaa
     5581 agatgcaagt gctcagcaag actcatatga atttgtttcc ccaagtcctc ctgcagatgt
     5641 ttctgagagg gctaaaaagg cttctgcaag atctggaaaa aagcaaaaaa agaaaacttt
     5701 agctgaaatc aaccaaaaat ggaatttaga ggcagaaaaa gaagatggtg aatttgactc
     5761 caaagaggaa tctaagcaaa agctggtatc cttctgtagc caaccatctg ttatctccag
     5821 tcctcagata aatggtgaaa tagacttact agcaagtggc tccttgacag aatctgaatg
     5881 ttttggaagt ttaactgaag tctctttacc attggctgag caaatagagt ctccagacac
     5941 taagagcagg aatgaagtag tgactcctga gaaggtctgc aaaaattatc ttacatctaa
     6001 gaaatctttg ccattagaaa ataatggaaa acgtggccat cacaatagac tttccagtcc
     6061 catttctaag agatgtagaa ccagcattct gagcaccagt ggagattttg ttaagcaaac
     6121 ggtgccctca gaaaatatac cattgcctga atgttcttca ccaccttcat gcaaacgtaa
     6181 agttggtggt acatcaggga ggaaaaacag taacatgtcc gatgaattca ttagtctttc
     6241 accaggtaca ccaccttcta cattaagtag ttcaagttac aggcgagtga tgtctagtcc
     6301 ctcagcaatg aagctgttgc ccaatatggc tgtgaaaaga aatcatagag gagagacttt
     6361 gctccatatt gcttctatta agggcgacat accttctgtt gaataccttt tacaaaatgg
     6421 aagtgatgca aatgttaaag accatgctgg atggacacca ttgcatgaag cttgcaatca
     6481 tgggcacctg aaggtagtgg aattattgct ccagcataag gcattggtga acaccaccgg
     6541 gtatcaaaat gactcaccac ttcacgatgc agccaagaat gggcacgtgg atatagtcaa
     6601 gctgttactt tcctatggag cctccagaaa tgctgttaat atatttggtc tgcggcctgt
     6661 cgattataca gatgatgaaa gtatgaaatc gctattgctg ctaccagaga agaatgaatc
     6721 atcctcagct agccactgct cagtaatgaa cactgggcag cgtagggatg gacctcttgt
     6781 acttataggc agtgggctgt cttcagaaca acagaaaatg ctcagtgagc ttgcagtaat
     6841 tcttaaggct aaaaaatata ctgagtttga cagtacagta actcatgttg ttgttcctgg
     6901 tgatgcagtt caaagtacct tgaagtgtat gcttgggatt ctcaatggat gctggattct
     6961 aaaatttgaa tgggtaaaag catgtctacg aagaaaagta tgtgaacagg aagaaaagta
     7021 tgaaattcct gaaggtccac gcagaagcag gctcaacaga gaacagctgt tgccaaagct
     7081 gtttgatgga tgctacttct atttgtgggg aaccttcaaa caccatccaa aggacaacct
     7141 tattaagctc gtcactgcag gtgggggcca gatcctcagt agaaagccca agccagacag
     7201 tgacgtgact cagaccatca atacagtcgc ataccatgcg agacccgatt ctgatcagcg
     7261 cttctgcaca cagtatatca tctatgaaga tttgtgtaat tatcacccag agagggttcg
     7321 gcagggcaaa gtctggaagg ctccttcgag ctggtttata gactgtgtga tgtcctttga
     7381 gttgcttcct cttgacagcc ccaagaagaa gaggaaagtc ggctctggcc ctgccgctaa
     7441 gagagtgaag ctggactaat taattaagct gccttctgcg gggcttgcct tctggccatg
     7501 cccttcttct ctcccttgca cctgtacctc ttggtctttg aataaagcct gagtaggaag
     7561 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     7621 ccctggaagg tgccactccc actgtccttt cctaataaaa tgagaaaatt gcatcgcatt
     7681 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     7741 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg
     7801 aaagaaccag ctggggctcg ataccgtcga cctctagcta gagcttggcg taatcatggt
     7861 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     7921 gaagcataaa gtgtaaagcc tagggtgcct aatgagtgag ctaactcaca ttaattgcgt
     7981 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     8041 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     8101 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     8161 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     8221 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     8281 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     8341 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     8401 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     8461 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     8521 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     8581 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     8641 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     8701 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     8761 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     8821 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     8881 acactcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     8941 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     9001 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     9061 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     9121 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     9181 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     9241 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     9301 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     9361 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     9421 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     9481 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     9541 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     9601 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     9661 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     9721 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     9781 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     9841 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     9901 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     9961 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg
    10021 gatcgggaga tcgatctccc gatcccctag ggtctactct cagtacaatc tgctctgatg
    10081 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
    10141 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
    10201 t
//
