LOCUS       sequence-501777-       10912 bp    DNA     circular SYN 04-MAR-2026
DEFINITION  Mammalian expression of cell-surface protein extracellular domain 
            fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10912)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 10912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 4, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-PTPRS-COMP5AP-AviTag-9xHis
FEATURES             Location/Qualifiers
     source          1..10912
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     124..143
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     149..168
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      254..842
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     743..762
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     996..1013
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     1111..1128
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1112..1128
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1584..1600
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     enhancer        1767..2146
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2147..2350
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2301..2321
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2347..2371
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        2581..2599
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2581..2598
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     2612..2631
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             complement(2950..2967)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             6517..6552
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             6562..6585
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             8245..8289
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             8290..8316
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
     primer_bind     complement(8456..8473)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8462..8573
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8691..8711)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8696..9025
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8876..9011
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8938..8957
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9073..9120
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9139..9738
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9139..9158)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9635..9655
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    9868..9989
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9905..9924)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9959..9978
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(10042..10833,10834..10902))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10665..10684
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(10903..10912,1..82))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
       61 tgtatttaga aaaataaaca aataggggtc agtgttacaa ccaattaacc aattctgaac
      121 attatcgcga gcccatttat acctgaatat ggctcataac accccttgct catgaccaaa
      181 atcccttaac gtgagttacg cgcgcgtcgt tccactgagc gtcagacccc gtagaaaaga
      241 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
      301 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
      361 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
      421 tagcccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
      481 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
      541 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
      601 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
      661 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      721 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      781 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      841 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      901 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      961 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1021 aaggcgagag tagggaactg ccaggcatca aactaagcag aaggcccctg acggatggcc
     1081 tttttgcgtt tctacaaact ctttctgtgt tgtaaaacga cggccagtct taagctcggg
     1141 ccccctgggc ggttctgata acgagtaatc gttaatccgc aaataacgta aaaacccgct
     1201 tcggcgggtt tttttatggg gggagtttag ggaaagagca tttgtcagaa tatttaaggg
     1261 cgcctgtcac tttgcttgat atatgagaat tatttaacct tataaatgag aaaaaagcaa
     1321 cgcactttaa ataagatacg ttgctttttc gattgatgaa cacctataat taaactattc
     1381 atctattatt tatgattttt tgtatataca atatttctag tttgttaaag agaattaaga
     1441 aaataaatct cgaaaataat aaagggaaaa tcagtttttg atatcaaaat tatacatgtc
     1501 aacgataata caaaatataa tacaaactat aagatgttat cagtatttat tatcatttag
     1561 aataaatttt gtgtcgccct taattgtgag cggataacaa ttacgagctt catgcacagt
     1621 ggcgttgact caatattggc cattagccat attattcatt ggttatatag cataaatcaa
     1681 tattggctat tggccattgc atacgttgta tctatatcat aatatgtaca tttatattgg
     1741 ctcatgtcca atatgaccgc catgttggca ttgattattg actagttatt aatagtaatc
     1801 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     1861 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     1921 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     1981 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     2041 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     2101 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     2161 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     2221 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     2281 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     2341 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
     2401 cctccataga agacaccggg accgatccag cctccgcggc cgggaacggt gcattggaac
     2461 gcggattccc cgtgccaaga gtgacgtaag taccgcctat agagtctata ggcccacccc
     2521 cttggcttcg ttagaacgcg gctacaatta atacataacc ttatgtatca tacacatacg
     2581 atttaggtga cactatagaa taacatccac tttgcctttc tctccacagg tgtccactcc
     2641 caggtccaac tgcacctcgg ttctatcgaa aacgcgcctg ctagcgccac catgaagacc
     2701 atcattgccc tgagctacat cttctgcctg gtgttcgctg cggccgccga agaacctcct
     2761 agattcatca aagagcccaa ggaccagatc ggcgttagcg gcggagttgc cagttttgtg
     2821 tgtcaggcca caggcgaccc taagcctaga gtgacctgga acaagaaggg caagaaagtc
     2881 aacagccagc gcttcgagac aatcgagttc gatgaatctg ccggcgctgt gctgagaatc
     2941 cagcctctga gaacccctcg ggacgagaat gtgtatgagt gcgtggccca gaacagcgtg
     3001 ggagagatta cagtgcacgc caagctgacc gtgctgagag aggatcagct gcccagcggc
     3061 ttccccaaca ttgatatggg acctcagctg aaggtggtgg aaagaaccag aaccgccaca
     3121 atgctgtgtg ccgcctctgg caatcctgat cctgagatta cctggttcaa ggacttcctg
     3181 cctgtggacc ctagcgccag caatggcaga atcaagcagc tgcggagcga gacattcgag
     3241 agcacaccta ttagaggcgc cctgcagatc gagagcagcg aggaaacaga ccagggcaaa
     3301 tacgagtgtg tggccaccaa tagcgctggc gtgcggtact ctagccctgc caatctgtat
     3361 gtgcgcgagc tgagagaagt gcggagagtg gccccaagat tcagcatcct gcctatgagc
     3421 cacgagatca tgcctggcgg caacgtgaac atcacatgtg tggccgtggg cagccccatg
     3481 ccttatgtga aatggatgca aggcgccgag gatctgaccc ctgaggatga tatgcctgtg
     3541 ggcagaaacg tgctggaact gaccgatgtg aaggacagcg ccaactacac atgcgtggcc
     3601 atgtctagcc tgggcgtgat cgaagccgtg gctcagatca cagtgaagtc cctgcctaag
     3661 gctcctggca cacccatggt tacagagaac accgccacca gcatcaccat cacctgggat
     3721 agcggaaacc ccgatcctgt gtcctactac gtgatcgagt acaagagcaa gagccaggac
     3781 ggcccctacc agatcaaaga ggacatcacc accaccagat acagcatcgg cggactgagc
     3841 cccaacagcg agtatgagat ttgggtgtcc gccgtgaact ccatcggaca gggacctcca
     3901 tctgagagcg tggtcacaag aacaggcgaa caggctcctg ccagcgctcc tagaaatgtg
     3961 caggccagaa tgctgagcgc caccaccatg atcgtgcagt gggaagaacc cgtggaaccc
     4021 aatggcctga tccggggcta cagagtgtac tacaccatgg aacctgagca ccccgtcggc
     4081 aactggcaga aacacaatgt ggacgacagc ctgctgacca ccgtgggatc tctgctggaa
     4141 gatgagacat acaccgtgcg cgtgctggcc tttacatctg ttggagatgg ccctctgagc
     4201 gaccccatcc aagtgaaaac acagcagggc gtgcccggcc agcctatgaa tcttagagcc
     4261 gaggccagaa gcgagactag catcacactt agctggtccc cacctcggca agagagcatc
     4321 attaagtacg agctgctgtt cagagagggc gaccacggaa gagaagtcgg cagaaccttt
     4381 gatcccacca cctcctacgt ggtggaagat ctgaagccca ataccgagta cgccttcaga
     4441 ctggccgcca gatctccaca aggactgggc gcttttacac ccgtcgtcag acagagaacc
     4501 ctgcagtcta aaccctccgc tccacctcag gatgtgaagt gcgtgtccgt gcggtctaca
     4561 gccattctgg tttcttggcg gcctcctcca cctgagacac ataatggcgc cctcgtgggc
     4621 tactctgtgc ggtatagacc tctgggcagc gaagatcccg agcctaaaga agtgaacggc
     4681 atccctccta ccacaacaca gattctgctc gaggccctgg aaaagtggac ccagtacaga
     4741 atcaccacag tggcccacac cgaagttggc cctggacctg aatcttctcc tgtggtcgtg
     4801 cggaccgatg aggatgttcc tagcgctcct cctaggaagg tggaagccga agctctgaat
     4861 gccaccgcca tcagagtgct ttggagaagc cctgctccag gcagacagca cggccagatt
     4921 agaggctacc aggtgcacta tgtgcggatg gaaggcgctg aagccagagg acctccacgg
     4981 atcaaggacg ttatgctggc cgatgctcag tgggagacag acgatacagc cgagtacgag
     5041 atggtcatca ccaacctgca gcctgagaca gcctacagca ttaccgtggc cgcctacaca
     5101 atgaaaggcg acggcgccag atccaagcct aaggtggtcg tgacaaaagg cgcagtgctg
     5161 ggcagaccta cactgtctgt tcagcagaca cctgagggca gtctgctggc tagatgggaa
     5221 cctcctgccg gaacagctga agatcaggtg ctgggataca gactgcagtt cggcagagag
     5281 gatagcaccc ctctggccac actggaattc cctccaagcg aggatagata caccgccagc
     5341 ggagtgcaca aaggcgccac atacgtgttc aggctggctg ctagaagcag aggcggactt
     5401 ggagaagagg ccgccgaggt tctgtctatc ccagaggata cacctcgggg acaccctcag
     5461 attctggaag ccgccggaaa tgcttctgct ggcacagttc tgctgagatg gctgcctcct
     5521 gtgcctgccg agagaaatgg cgctatcgtg aagtacactg tggccgtcag agaagctggt
     5581 gccctgggac ctgctagaga gacagaactt cctgctgctg ccgagcctgg cgccgaaaat
     5641 gctcttacac tgcaaggact gaagcccgac accgcctatg atctgcaagt gcgggcccac
     5701 acaagaagag gacctggacc ttttagccca ccagtgcggt acaggacctt cctgagagat
     5761 caagtgtccc ctaagaactt caaagtgaag atgatcatga agaccagcgt gctgctgagc
     5821 tgggagttcc ccgacaacta caacagcccc acaccttaca agatccagta caacggactg
     5881 accctggacg tggacggcag gaccacaaag aagctgatca cccacctgaa gccacacacc
     5941 ttctacaact tcgtgctgac caacagaggc agctctctcg gaggactgca gcagacagtt
     6001 acagcctgga ccgccttcaa cctgctgaat ggcaaaccta gcgtggcccc taaacctgac
     6061 gccgatggct tcatcatggt gtacctgcct gatggccagt ctccagtgcc tgtgcagagc
     6121 tacttcatcg tgatggtgcc cctgagaaag agtagaggcg ggcagtttct gaccccactg
     6181 ggatctcccg aggacatgga tctggaagaa ctgatccagg acatctcccg gctgcagaga
     6241 agaagcctga ggcacagtag acagctggaa gtgcccagac cttatatcgc cgccaggttt
     6301 tctgtgctgc ctccaacatt tcaccccggc gatcagaagc agtacggcgg ctttgacaac
     6361 agaggactgg aacccggcca cagatacgtg ctgtttgtgc tggcagtgct gcagaagtcc
     6421 gagcctacat tcgccgcctc tccattcagc gacccctttc agctggacaa ccctgatcca
     6481 cagcctatcg tggatggcga agagggtggc gcgccggaag atcaagttga tccaagattg
     6541 attgatggca agggcggatc tctggaggtg ctgttccagg gaccaggcgg aagcggcaat
     6601 cccggcggcg atctggcccc ccagatgctg cgcgagctgc aggagaccaa tgccgccctg
     6661 caggacgtgc gcgagctgct gcgccaccgc gtgaaggaga tcaccttcct gaagaacacc
     6721 gtgatggagt gcgacgcctg cggtatgcag ccctctagag gctccggctc catcatccca
     6781 gttgaggagg agaacccgga cttctggaac cgcgaggcag ccgaggccct gggtgccgcc
     6841 aagaagctgc agcctgcaca gacagccgcc aagaacctca tcatcttcct gggcgatggg
     6901 atgggggtgt ctacggtgac agctgccagg atcctaaaag ggcagaagaa ggacaaactg
     6961 gggcctgaga tacccctggc catggaccgc ttcccatatg tggctctgtc caagacatac
     7021 aatgtagaca aacatgtgcc agacagtgga gccacagcca cggcctacct gtgcggggtc
     7081 aagggcaact tccagaccat tggcttgagt gcagccgccc gctttaacca gtgcaacacg
     7141 acacgcggca acgaggtcat ctccgtgatg aatcgggcca agaaagcagg gaagtcagtg
     7201 ggagtggtaa ccaccacacg agtgcagcac gcctcgccag ccggcaccta cgcccacacg
     7261 gtgaaccgca actggtactc ggacgccgac gtgcctgcct cggcccgcca ggaggggtgc
     7321 caggacatcg ctacgcagct catctccaac atggacattg acgtgatcct aggtggaggc
     7381 cgaaagtaca tgtttcgcat gggaacccca gaccctgagt acccagatga ctacagccaa
     7441 ggtgggacca ggctggacgg gaagaatctg gtgcaggaat ggctggcgaa gcgccagggt
     7501 gcccggtatg tgtggaaccg cactgagctc atgcaggctt ccctggaccc gtctgtgacc
     7561 catctcatgg gtctctttga gcctggagac atgaaatacg agatccaccg agactccaca
     7621 ctggacccct ccctgatgga gatgacagag gctgccctgc gcctgctgag caggaacccc
     7681 cgcggcttct tcctcttcgt ggagggtggt cgcatcgacc atggtcatca tgaaagcagg
     7741 gcttaccggg cactgactga gacgatcatg ttcgacgacg ccattgagag ggcgggccag
     7801 ctcaccagcg aggaggacac gctgagcctc gtcactgccg accactccca cgtcttctcc
     7861 ttcggaggct accccctgcg agggagctcc atcttcgggc tggcccctgg caaggcccgg
     7921 gacaggaagg cctacacggt cctcctatac ggaaacggtc caggctatgt gctcaaggac
     7981 ggcgcccggc cggatgttac cgagagcgag agcgggagcc ccgagtatcg gcagcagtca
     8041 gcagtgcccc tggacgaaga gacccacgca ggcgaggacg tggcggtgtt cgcgcgcggc
     8101 ccgcaggcgc acctggttca cggcgtgcag gagcagacct tcatagcgca cgtcatggcc
     8161 ttcgccgcct gcctggagcc ctacaccgcc tgcgacctgg cgccccccgc cggcaccacc
     8221 gacgccgcgc acccgggtgg cggaggactg aacgacatct tcgaggctca gaaaatcgaa
     8281 tggcacgaac atcaccatca ccatcaccat caccattaag gccggcctgt agtcactggc
     8341 acaacgcgtt acccggtaag ccaatcgggt atacacggtc gtcatactgc agacagggtt
     8401 cttctacttt gcaagatagt cttgagtagt aaaataaata gatagagaaa atcagcctcg
     8461 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     8521 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcacaacac
     8581 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     8641 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg
     8701 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     8761 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     8821 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     8881 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     8941 ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt
     9001 ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc
     9061 tgatcagcac gtgttgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac
     9121 aaggtgagga actaaaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
     9181 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
     9241 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
     9301 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
     9361 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
     9421 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
     9481 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
     9541 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
     9601 ggggtgcccg ccttcctgga gacatccgcg ccccgcaacc tccccttcta cgagcggctc
     9661 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
     9721 cgcaagcccg gtgcctgaca cgtgctacga gatttcgatt ccaccgccgc cttctatgaa
     9781 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
     9841 ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa
     9901 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     9961 ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag
    10021 agcttggcgt aatcatggtc attaccaatg cttaatcagt gaggcaccta tctcagcgat
    10081 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
    10141 ggagggctta ccatctggcc ccagcgctgc gatgataccg cgagaaccac gctcaccggc
    10201 tccggattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
    10261 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
    10321 gccagttaat agtttgcgca acgttgttgc catcgctaca ggcatcgtgg tgtcacgctc
    10381 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
    10441 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
    10501 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
    10561 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
    10621 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
    10681 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
    10741 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
    10801 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
    10861 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atattcttcc tt
//
