LOCUS       sequence-501813-        3523 bp    DNA     circular SYN 04-MAR-2026
DEFINITION  Level 0 Golden Gate part Barley Oleosin terminator, GCTT - CGCT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3523)
  TITLE     Plant Golden-gate Promoters, Terminators and Level0/Level1 cloning 
            acceptors.
REFERENCE   2  (bases 1 to 3523)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 4, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pICSL60020
FEATURES             Location/Qualifiers
     source          1..3523
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(2150..2168)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2236..2340
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2562..3353
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tcactctgtg gtctcagctt
      841 gctggaggta gcagaccgat ggatgtcagt cggtcggccg cgccgcctgc catgtgccag
      901 tgccttccta gtacgtacgt gtctagggcg tccgtctccc cgagcgaggg cgcgtcaacg
      961 tgtcacgtgt gtgtgtgttc gtgcgtgtca gagagtgtgt gatgtttctc tacttgcctg
     1021 cctgcctgcc tgcctgctta gagttgtact actgctcatg tttggatgga tcgatcggtt
     1081 tagcaccgag tttgtgaata agaaaaggcc ggggcagctt cagtttgttc atgcatttgc
     1141 ttctgtgctc gatgccatcc tcgagatttt cgttttcacc cacgacgtgc tacgatagaa
     1201 tcagagggtg gaagcaggtg ctcgccgtcg tagcaaacgt tgccgttgca ggtagcacct
     1261 gtccagcaag tcgcggcctc gcagctctac cgggtgctcg ccggtcaccg tcacatgcag
     1321 cacctatccg gctctgtttg ctgctctacc ggccctcatt tgcagcttgc agagcgtcgg
     1381 tcgtcgccct tcacaagtta taagggtgat gcttaagttt tgcatgtatc tattcctaaa
     1441 aacatggcga gagttattgt gcttgttagg aataaaatat ctttagattt gagttaatta
     1501 gcacatccct tagaatattc tatttagcag ttaaaatatc tttagatttg agttaattag
     1561 gcaattagca cattccttag aatattctat ttagcagtta gaatattggc tttgtccata
     1621 tgttgtgtgc ctcaaaccgt ggcacccatc tcccaaaccg tctcacgcga ctattacatg
     1681 tactcataac tgttcacgat atataaatac ctacagtcac tatcgatgaa atcactttat
     1741 tcattttaat ttttttttct ttttacgata tcatgcacgc atcgttctag tacagagagc
     1801 tagtacagag agagaataga ctacaacaaa gaagaaaggt gagaagagat ggatacagga
     1861 aagaagaaag gtgagaagag atggattctt tcacggacct tgcttttgct tttgctaggt
     1921 ttgtggctag ggagcgttat gttttccttc ttcttcggcg gacctttggt ctggccgccg
     1981 ctggaacttc ggtcaccgcc attagcgtag cccaggaacg acgatctccg tcactgcgag
     2041 gcaccatatc gtgttcctcg ttgtcgatat ggccctcgtg gtggcctccg ttgccgccat
     2101 gtctatcgcc cgcttgagac cacgaagtgg ctcttcagtg gacgaaaggg cctcgtgata
     2161 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     2221 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     2281 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     2341 atgcgctcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg
     2401 gcggttttca tggcttgtta tgactgtttt tttttggggt acagtctatg cctcgggcat
     2461 ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg
     2521 ttacgcagca gggcagtcgc cctaaaacaa agttaaacat catgagggaa gcggtgatcg
     2581 ccgaagtatc gactcaacta tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga
     2641 cgttgctggc cgtacatttg tacggctccg cagtggatgg cggcctgaag ccacacagcg
     2701 atattgattt gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga
     2761 tcaacgacct tttggaaact tcggcttccc ctggagagag cgagattctc cgcgctgtag
     2821 aagtcaccat tgttgtgcac gacgacatca ttccgtggcg ttatccagct aagcgcgaac
     2881 tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg tatcttcgag ccagccacga
     2941 tcgacattga tctggctatc ttgctgacaa aagcaagaga acatagcgtt gccttggtag
     3001 gtccagcggc ggaggaactc tttgatccgg ttcctgaaca ggatctattt gaggcgctaa
     3061 atgaaacctt aacgctatgg aactcgccgc ccgactgggc tggcgatgag cgaaatgtag
     3121 tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg
     3181 tcgctgccga ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc atacttgaag
     3241 ctagacaggc ttatcttgga caagaagaag atcgcttggc ctcgcgcgca gatcagttgg
     3301 aagaatttgt ccattacgta aaaggcgaga tcaccaaggt agtcggcaaa taactgtcag
     3361 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     3421 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     3481 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttc
//
