LOCUS       sequence-501821-        9262 bp    DNA     circular SYN 04-MAR-2026
DEFINITION  Dual color expression of mCherry tagged human Sec 61B and EGFP 
            tagged zebrafish LaminB1 in mammalian cell lines.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9262)
  AUTHORS   Shen Z, Gelashvili Z, Niethammer P
  TITLE     Endoplasmic reticulum disruption stimulates nuclear membrane 
            mechanotransduction.
  JOURNAL   Nat Cell Biol. 2025 Dec 9. doi: 10.1038/s41556-025-01820-9.
  PUBMED    41366519
REFERENCE   2  (bases 1 to 9262)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 4, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2025 Dec 9. doi: 10.1038/s41556-025-01820-9."
COMMENT     Sequence Label: pSB-CMV-MCS-Puro mCherry-Sec 61B -P2A-EGFP-LMNB1
FEATURES             Location/Qualifiers
     source          1..9262
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        10..389
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        390..593
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     544..564
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        638..656
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      676..685
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             682..1389
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(827..845)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1095..1114
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             1405..1692
                     /codon_start=1
                     /gene="SEC61B"
                     /product="human Sec61-beta subunit of the translocon in the
                     endoplasmic reticulum"
                     /label=Sec61-beta
                     /translation="MPGPTPSGTNVGSSGRSPSKAVAARAAGSTVRQRKNASCGTRSAG
                     RTTSAGTGGMWRFYTEDSPGLKVGPVPVLVMSLLFIASVFMLHIWGKYTRS"
     CDS             1717..1773
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(1780..1782,1783..1785,1786..2496)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1780..1782,1783..1785,1786..2496)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1825..1846)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2086..2105)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2433..2454
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4301..4318)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4307..4531
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        4538..4895
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4538..4729
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4561..4581)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4746..4881
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4923..5522
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4923..4942)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5419..5439
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(5536..5555)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     polyA_signal    5565..5646
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     5590..5609
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(6121..6141)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6121..6139)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6160..6176)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6160..6176)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6173..6195)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6184..6200
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6208..6214,6215..6232,6233..6238))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6253..6274
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6391..6408)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6562..7150)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6642..6661)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7321..8112,8113..8181))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAAKLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7944..7963
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8182..8286)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      8313..8768
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8400..8419)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8610..8631
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8894..8916
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8908..8925
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8909..8925
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        8935..8953
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 aagcttgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
       61 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
      121 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
      181 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
      241 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
      301 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
      361 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
      421 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
      481 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
      541 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc
      601 taactagaga acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga
      661 cccaagctgg ctagcgccac catggtgagc aagggcgagg aggataacat ggccatcatc
      721 aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag
      781 atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg
      841 accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc
      901 tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc
      961 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc
     1021 caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac
     1081 ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag
     1141 cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag
     1201 gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag
     1261 ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac
     1321 accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag
     1381 ctgtacaagt ccggactcag atctatgcct ggtccgaccc ccagtggcac taacgtggga
     1441 tcctcagggc gctctcccag caaagcagtg gccgcccggg cggcgggatc cactgtccgg
     1501 cagaggaaaa atgccagctg tgggacaagg agtgcaggcc gcacaacctc ggcaggcacc
     1561 ggggggatgt ggcgattcta cacagaagat tcacctgggc tcaaagttgg ccctgttcca
     1621 gtattggtta tgagtcttct gttcatcgct tctgtattta tgttgcacat ttggggcaag
     1681 tacactcgtt cgtcgaattc ccgggtcgga agcggagcta ctaacttcag cctgctgaag
     1741 caggctggag acgtggagga gaaccctgga cctagatcca tggtgagcaa gggcgaggag
     1801 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
     1861 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
     1921 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac
     1981 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
     2041 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
     2101 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     2161 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
     2221 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag
     2281 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
     2341 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc
     2401 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     2461 gccgggatca ctctcggcat ggacgagctg tacaagagat ctatggcgag cgcggcgtgt
     2521 actccggccg ggcagcagtc gttgtcccgc ggcaccagca gcactccact gagccccgcg
     2581 cggatctcca ggctgcaaga gaaagaggag ctgcagcagc tcaatgaccg gcttgccgcc
     2641 tacatcgaca cagtccgtag tctggagtcc gagaacagcg tcctgcaaat acagatcaga
     2701 gaacgagagg atgtgcggaa ccgcgagctc acggggctca gaacactcta cgaaaccgag
     2761 ctggcggacg cacggaaggc gcttgatgac acggcacggg agcgcgcacg attgcagatt
     2821 gaactgggca agattaaaag cgagcaagag cagctacagc acacatatgc caagaaagat
     2881 gctgacctca ccagctctca gaaccgacta aaagatgttg aagccctgtt gaacgctaaa
     2941 gaagctgcac taaacacagc cgtctctgaa aggaagactc tggaaaacac tctatctgat
     3001 ctgcagatcc gtgtccagga gttggaagga gggttgatct ccgctaagaa gcagctgtca
     3061 gatgagaccc tgctgcgtgt ggatctggag aaccgctgtc agagccttat ggaggaactt
     3121 gaatttagga aaaacatgtt tgaagaggaa atcaaagata cacgtcgtag acatgagacc
     3181 cgtcttgtcg aggtggattc aggtcgtcag atggagtatg agtttaaact ggctcaggct
     3241 ctgactgaca tgagacagca gcatgatgag caagtcaaac tttataagga ggagatggag
     3301 cagacatacg tgtccaagct ggagaacatt cgtttgtcat ctgagatgaa cagctcttca
     3361 gctagtatgg cccgtgagga gcttcgagag tctacactga gagttgagag tttggcaggt
     3421 caacttgcaa atctgcagaa agaggctcgt gcctggcagg atcgcattaa tgaactggag
     3481 ggggctctta gccatgagaa agatttaagc cgtaggttgc tggcagagaa agagcgcgag
     3541 attgctgaga tcagagccaa gatgcagcag cagctggatg agtatgagca actgctggac
     3601 gtcaaactag cgctggacat ggagatcaat gcttacagaa agcttctgga gggagaagaa
     3661 gaaaggttga aactgtctcc aagtccttca tctcgtgtga ctgtgtcacg agcatcctcc
     3721 agtcgcagtg ttcgtactac ccgtggcaag agaaagcgtg tggatgtgga ggagtcggag
     3781 gcaagcagca gtgtcagtat cgctcattct gcatctgcca ctggaaacgt ctccatcgat
     3841 gagctggatg tagatgggaa gttcatccgc ttgcacaaca actctgagca ggaccagccg
     3901 atggcaggat ttcagctgac caggactatt ggagaagtta ctgcaacata taaattcact
     3961 gcaaaataca atctgaaggc tggacagaaa gtgacgatct gggcatctaa tgcaggcata
     4021 agctccaacc ctcctgctga cctgatatgg aagaaccagc cttcatgggg gactggggag
     4081 aacataaaag tggctctttt gagcccagca ggagaggaag tagctgtgag aagcacagta
     4141 ttcaagactg cagtggaaga tgaagacgac gatgaggagg aagaaaagga agctgaagtc
     4201 attgaggagc atttctatca ccagcaggga gatccacgcg ctgctaagag aggatgtttg
     4261 atcatgtaat ctagagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt
     4321 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     4381 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     4441 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
     4501 aggcatgctg gggatgcggt gggctctatg gaccggactg aggcggaaag aaccagctgt
     4561 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     4621 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
     4681 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
     4741 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     4801 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     4861 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct agattttctg accctagcca
     4921 ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc ccccgggccg
     4981 tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgacccgg
     5041 accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg
     5101 acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg
     5161 agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg
     5221 gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg
     5281 agcccgcgtg gttcctggcc accgtcggcg tgtcgcccga ccaccagggc aagggtctgg
     5341 gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc
     5401 tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg
     5461 ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct
     5521 gaggatctcg agctcgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc
     5581 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa
     5641 caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag
     5701 gttttttaaa gcaagtaaaa cctctacaaa tgtggtactt aagagggatc gataaggatc
     5761 tagcttgtgg aaggctactc gaaatgtttg acccaagtta aacaatttaa aggcaatgct
     5821 accaaatact aattgagtgt atgtaaactt ctgacccact gggaatgtga tgaaagaaat
     5881 aaaagctgaa atgaatcatt ctctctacta ttattctgat atttcacatt cttaaaataa
     5941 agtggtgatc ctaactgacc taagacaggg aatttttact aggattaaat gtcaggaatt
     6001 gtgaaaaagt gagtttaaat gtatttggct aaggtgtatg taaacttccg acttcaactg
     6061 tatagggatc ctctagctag agtcgacctc gagggggggc ccggtaccca gcttttgttc
     6121 cctttagtga gggttaattg cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     6181 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     6241 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     6301 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     6361 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     6421 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     6481 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     6541 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     6601 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     6661 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     6721 cgcctttctc ccttcgggaa ccgtggcgct ttctcatagc tcacgctgta ggtatctcag
     6781 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     6841 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     6901 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     6961 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     7021 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     7081 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     7141 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     7201 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     7261 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     7321 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     7381 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     7441 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     7501 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     7561 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     7621 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     7681 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     7741 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaac ttggccgcag tgttatcact
     7801 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     7861 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     7921 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     7981 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     8041 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     8101 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     8161 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     8221 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     8281 tccgcgcaca tttccccgaa aagtgccacc tgacgcgccc tgtagcggcg cattaagcgc
     8341 ggcgggagtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     8401 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     8461 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     8521 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     8581 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     8641 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     8701 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct
     8761 tacaatttcc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc
     8821 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta
     8881 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg cgcgtaatac
     8941 gactcactat agggcgaatt ggagctcgga tccctataca gttgaagtcg gaagtttaca
     9001 tacacttaag ttggagtcat taaaactcgt ttttcaacta ctccacaaat ttcttgttaa
     9061 caaacaatag ttttggcaag tcagttagga catctacttt gtgcatgaca caagtcattt
     9121 ttccaacaat tgtttacaga cagattattt cacttataat tcactgtatc acaattccag
     9181 tgggtcagaa gtttacatac actaagttga ctgtgccttt aaacagcttg gaaaattcca
     9241 gaaaatgatg tcatggcttt ag
//
