LOCUS       sequence-501843-        5636 bp    DNA     circular SYN 04-MAR-2026
DEFINITION  Control plasmid for null expression of recombinant protein when 
            compared to pTA-M+ (see Table 1 of publication).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5636)
  AUTHORS   Sandhaus S, Annamalai T, Welmaker G, Houghten RA, Paz C, Garcia PK, 
            Andres A, Narula G, Rodrigues Felix C, Geden S, Netherton M, Gupta 
            R, Rohde KH, Giulianotti MA, Tse-Dinh YC
  TITLE     Small-Molecule Inhibitors Targeting Topoisomerase I as Novel 
            Antituberculosis Agents.
  JOURNAL   Antimicrob Agents Chemother. 2016 Jun 20;60(7):4028-36. doi: 
            10.1128/AAC.00288-16. Print 2016 Jul.
  PUBMED    27114277
REFERENCE   2  (bases 1 to 5636)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 4, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/AAC.00288-16"; journalName: "Antimicrob Agents Chemother"; 
            date: "2016-06-20- 20"; volume: "60"; issue: "7"; pages: "4028-36"
COMMENT     Sequence Label: pTA-noI
FEATURES             Location/Qualifiers
     source          1..5636
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     2165..2181
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     2166..2182
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(2726..2753)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(2843..2860)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3014..3602)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3094..3113)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 caacgagaca ggcatggccc aaaccagaaa actagcgtct accaggactt ttacctgtcc
       61 gacccgttgc aacggaaccc cccacggaac ccccgcgaca cccgctcccc aattgcgtta
      121 gaacagcggt ggattgtcgg cttcgttgtg ggccttttga gccgcttcct gttctgccgc
      181 acgctctttc ctcgcccgat agccgagtcg cttaacggtg tccagatgca gcccgaaatg
      241 tttggccgtt tgcggccaag agtggccctc gtcgtcgtga taggcgcgga tgcgttcgcg
      301 gcgtgcagcc tgctcggcga gccactcgct gcgttcctgc gccacgagcc ggacgacgtg
      361 gcgttcggat agtccggtga ttcgagcgcc ttcggcggcg gtcacgcgcc gctttttgcg
      421 gacagtcggc tgccggttgt agccgtcgct gtagccgtcg ctcatagcaa tgcctccatg
      481 gctgacgcgg actttgcgcg ccgcgcaact gtgctcgccg ccgtgcgcgc tgctgcgccc
      541 ttccgcgaga tggccgactg gcgcgcactg agtgtggcct cgtagaccac gatcccgtcc
      601 gcccaaatgc gcgacttggt tgtgatccaa cgccaaatgc tgttggcgat ggcgcggacc
      661 tcgctgtccg gtagcggtcc gggacacacg tcgttgcacg ggaattcggc gtttcgcgcg
      721 tggcactcgg catagatcgc gcggccgagt ccgtccacgt tccgggtcgg caggtagatc
      781 cgcatgaggg cgggacgata ggcccacaac ctgacggaat cgaacagtgc gcaattccgc
      841 cctagcggcg tcggagccgc tttgtacgtg gtctgctgac gccagcgcgg cggtggcatg
      901 ttcgcgccga gctcggcctc gatgtggctg agtgtgtaga gatctgagtg gagccattcc
      961 gtttcccagg cgatgtggcc ggggtttttg gtcatgaggc ctgagtaact gcggtcgccg
     1021 tcgacggcgc gccgaaggcc ttcggcgcac gccgccatgt atgcgagcgg cttacgccgc
     1081 gcgtattcgg tgcgtggaac aggggcgttg agtgcccaca ctgcgtgtgc gtggccgttg
     1141 gcgcgattgc ccacgatcgc gttgggcagc ggatgggacc cccgggcgct gagcgctcgg
     1201 agcgctgcgt ctggatggtc tacgtccacg accagcaggt ttgccagcgc tgttgggttc
     1261 gcctcgatgt accggcggcc tagggccgac gcgcggcttt ggcggtagat cccctcgagc
     1321 agatcgtcgc ttgccagcgg ccagtacggc agccagagct gctcaaattc gtcggcgacg
     1381 tggctcacgc ttggtagtag accacgatta atcaccggtg tatggtccga cacgagctcc
     1441 aagtcagata tttcgctgag gggccacccc acaactgcac actcccccgc tctcccgtcg
     1501 agccctggtg gtggaacacc agcgacagcc gagcaccccc aaccacctgt accaaccagg
     1561 aggaacacat gcgtcgtttc gaggacgttt ccgggccgct gagagccgct gtggcggccg
     1621 tacacgccgc cttagacccg ttagaccccc tgccgcctga atgcgcgggt acgagccaca
     1681 cagcgcccga acttacggag ctggtgggct cacctggctt tatggcgtac gaatcggctg
     1741 tgtgcgacct gttgggcgag gtgaggtacg cgctactcac gctggcaagg gcgacagagc
     1801 cgccccaccg agcccgcacg gccgcgcgcg gtgtcaacaa ccgggtgagt cgtgcacacc
     1861 agcaggtgtt cgaggcttgg ctcgaagtgc aggacatcgt ggcgaacgcc gcccgatgag
     1921 ccgcgcctta cgctggctgc cagccgttcg cgggctggtt ggtgcagcgc gtcgagcggt
     1981 tagaggccct gcggtgttcc accaccgcag ggcctcgccc tttttaaggc tgaatttgct
     2041 tgtctccgaa tccaactggc ttgtccaagg gtgtatctac gcttaatcca aagttcaaac
     2101 gaggggatta cacatgacca acttcgataa cgttctcggc tcgatgatcc ccgggggccc
     2161 cccctcgagg tcgacggtat cgataaaata aaaaagggga cctctagggt ccccaattaa
     2221 ttagctaaag cttttacgag tggtatttga cgatgttggc gtttaccaca tcgataaccg
     2281 ctctttgctg cgctttatct tccatcgact caaaagacat caataggtcc gcggcaccac
     2341 aacactcaat cgcaactcgc gctgcctgag tcacgcacta cacgcggtcc gtgacggtgc
     2401 gcgtctccac gctgagcacc accgcagacc atccggcgac gacaccgacg aaggagctga
     2461 ggcttccggt cagcaagtcg ggcttctcgt gtctcaggga ttcgatgaac agacagccat
     2521 cagcatgctc atcgcggtca gccgatacac cgtcggtgtc gtactggagg agcaaaccgc
     2581 agaggtcggc aatgcgagcg ttcctgaacg cgaccgggaa ttcgatttcg gcctgacagc
     2641 actgatcgac ggattctctc actcccgcac agcgtcattg acctcgagcg aatcgtgatc
     2701 tagagtttaa acagtattaa acgcaaaaag gccatccgtc aggatggcct tctgcttagc
     2761 taatcaacta gttctagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     2821 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     2881 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     2941 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     3001 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     3061 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     3121 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     3181 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     3241 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     3301 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     3361 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     3421 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     3481 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     3541 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     3601 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     3661 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     3721 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     3781 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     3841 gactccccga cgtggccgac cagcccgtca tcgtcaacgc ctgatccgcg gtgcggacag
     3901 gccgtgtcgc gaccggccgt gcggaattaa gccggcccgt accctgtgaa tagaggtccg
     3961 ctgtgacaca agaatccctg ttacttctcg accgtattga ttcggatgat tcctacgcga
     4021 gcctgcggaa cgaccaggaa ttctgggagc cgctggcccg ccgagccctg gaggagctcg
     4081 ggctgccggt gccgccggtg ctgcgggtgc ccggcgagag caccaacccc gtactggtcg
     4141 gcgagcccgg cccggtgatc aagctgttcg gcgagcactg gtgcggtccg gagagcctcg
     4201 cgtcggagtc ggaggcgtac gcggtcctgg cggacgcccc ggtgccggtg ccccgcctcc
     4261 tcggccgcgg cgagctgcgg cccggcaccg gagcctggcc gtggccctac ctggtgatga
     4321 gccggatgac cggcaccacc tggcggtccg cgatggacgg cacgaccgac cggaacgcgc
     4381 tgctcgccct ggcccgcgaa ctcggccggg tgctcggccg gctgcacagg gtgccgctga
     4441 ccgggaacac cgtgctcacc ccccattccg aggtcttccc ggaactgctg cgggaacgcc
     4501 gcgcggcgac cgtcgaggac caccgcgggt ggggctacct ctcgccccgg ctgctggacc
     4561 gcctggagga ctggctgccg gacgtggaca cgctgctggc cggccgcgaa ccccggttcg
     4621 tccacggcga cctgcacggg accaacatct tcgtggacct ggccgcgacc gaggtcaccg
     4681 ggatcgtcga cttcaccgac gtctatgcgg gagactcccg ctacagcctg gtgcaactgc
     4741 atctcaacgc cttccggggc gaccgcgaga tcctggccgc gctgctcgac ggggcgcagt
     4801 ggaagcggac cgaggacttc gcccgcgaac tgctcgcctt caccttcctg cacgacttcg
     4861 aggtgttcga ggagaccccg ctggatctct ccggcttcac cgatccggag gaactggcgc
     4921 agttcctctg ggggccgccg gacaccgccc ccggcgcctg acgccccggg ccgcccggcg
     4981 ccgcccccgg cccccggcgg ccgccgggcg gagccccgcc cggcggcccg ggagccccgg
     5041 gcccgcgccg aagcccgctg ctgcgagccc ggagcgggcc ggccgacggc ggtgcgggcc
     5101 cggcggcgga cgctcagcag cggcgggcgt gaaaggccct ggcatcctcg atcatctcct
     5161 ccagggtggg tcggccgggc ttccatccca gctcggcaag gatcaacgag gacagtcgca
     5221 cgacgaagtt cttctggatc gcgcccgtgc tggaagcact caacctcgaa gcgtgtggtt
     5281 gcggagccat ctagcaacca cacgaaacat gcgcaacgaa ccgcgcaacg aacaacgcct
     5341 agaactggcc ctagatgagc tgactcgtat cgttggtaaa cctagtttga ccagcatgtt
     5401 ttaactacgt tcggtgagct gtcaacgggg cctgtaacgg cacaacgaac cgtgcaacga
     5461 gagtggccac ggatgccacc acaggcacta caacggagtt cgccacgtac atcaccacaa
     5521 ccaccgattc tggcggtgag ctccccgata ttcagcggaa atggcttggt atcgaccaag
     5581 attcgtagaa ccccgtctcg tctggctggt attcaaaacg ggcgcaacga aacacg
//
