LOCUS       sequence-501925-        5972 bp    DNA     circular SYN 04-MAR-2026
DEFINITION  CytoTape-vivo structural monomer for CytoTape-vivo fiber formation 
            and elongation for mouse brain in vivo applications.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5972)
  AUTHORS   Zheng L, Shi D, Yan Y, Zhou B, Lim J, Hou Y, An B, Adhinarta JK, Lin
            M, Ko B, Joesten WC, Gautam M, Huez EDM, Kim EC, Klyder EG, Chang B,
            Pitchiaya S, Roberts MT, Cai DJ, Boyden ES, Wei D, Lio P, Linghu C
  TITLE     Scalable and multiplexed recorders of gene regulation dynamics 
            across weeks.
  JOURNAL   Nature. 2026 Jan 26. doi: 10.1038/s41586-026-10156-9.
  PUBMED    41588170
REFERENCE   2  (bases 1 to 5972)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 4, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2026 Jan 26. doi: 10.1038/s41586-026-10156-9."
COMMENT     Sequence Label: pAAV-UbC-CytoTape-vivo-HA
FEATURES             Location/Qualifiers
     source          1..5972
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   72..201
                     /label=AAV2 ITR
     promoter        274..672
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     CDS             2973..2999
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    3024..3231
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   3401..3530
                     /label=AAV2 ITR
     rep_origin      complement(3767..4355)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3847..3866)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4526..5317,5318..5386))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5149..5168
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5387..5491)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5517..5972)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5654..5675)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5866..5885
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
       61 tattacgcca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
      121 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      181 actccatcac taggggttcc ttgtagttaa tgattaaccc gccatgctac ttatctacgt
      241 agccatgctc taggaagatc gtaggttaat taaggcctcc gcgccgggtt ttggcgcctc
      301 ccgcgggcgc ccccctcctc acggcgagcg ctgccacgtc agacgaaggg cgcagcgagc
      361 gtcctgatcc ttccgcccgg acgctcagga cagcggcccg ctgctcataa gactcggcct
      421 tagaacccca gtatcagcag aaggacattt taggacggga cttgggtgac tctagggcac
      481 tggttttctt tccagagagc ggaacaggcg aggaaaagta gtcccttctc ggcgattctg
      541 cggagggatc tccgtggggc ggtgaacgcc gatgattata taaggacgcg ccgggtgtgg
      601 cacagctagt tccgtcgcag ccgggatttg ggtcgcggtt cttgtttgtg gatcgctgtg
      661 atcgtcactt ggcgactagt gccaccatga ttgactatac tgctgctggt ttcaccctcc
      721 tccaaggtgc acatctgtac gctcctgaag acagaggtat ctgcgatgtg ctggttgcca
      781 acggtaagat catcgctgtg gcctccaaca ttcctagcga catcgttcca aactgcaccg
      841 ttgtcgatct gagcggacag atcctctgtc caggattcat tgatcagcac gtgcacctca
      901 ttggaggagg aggcgaagct ggtcctacta ctagaactcc cgaagtcgca ctgtctcgcc
      961 tcacagaggc aggcgttacc tccgtcgtgg gactcctggg tactgatagc atctctaggc
     1021 accctgagtc tctgctggca aagaccagag ccctcaatga ggagggtatc tccgcttgga
     1081 tgctgactgg tgcttatcac gtcccatctc gcaccattac tggcagcgtg gagaaggacg
     1141 ttgcaatcat cgacagagtg atcggtgtta agtgcgctat ctccgaccac aggagcgctg
     1201 ctcccgacgt ctatcacctg gccaacatgg cagccgagag cagagtcggt ggtctgctcg
     1261 gtggcaagcc aggagttacc gtgttccaca tgggtgactc taagaaggca ctgcaaccca
     1321 tctatgacct gctggagaac tgtgacgtcc caatctccaa gctgctgcct acccacgtca
     1381 acaggaacgt gcctctcttc taccaagctc tggagttcgc aagaaagggt ggcaccatcg
     1441 acatcacctc tagcattgat gaacccgtcg ctccagctga gggaatcgca agggctgtcc
     1501 aagcaggtat cccactggca agggtgacac tcagctctga tggcaacggc tcccagccat
     1561 tcttcgatga tgaaggaaat ctgactcaca ttggcgtggc tggatttgaa actctcctgg
     1621 aaacagttca ggttctggtt aaggactacg acttcagcat ttctgacgct ctcagaccac
     1681 tcacctctag cgtcgctggc ttcctgaacc tgactggtaa aggcgaaatc aaacctggta
     1741 atgacgctga tctgctggtg atgacacccg aactgcgcat tgagcaggtc tatgcaagag
     1801 gaaagctgat ggttaaggac ggaaaggctt gtgtcaaggg cacattcgag actgctggtg
     1861 gctctggagg caccaagatt gaagagggca aactggttat ctggatcaat ggcgataagg
     1921 gctacaatgg cctggcagag gtcggtaaga agttcgagaa agatacaggc atcaaagtta
     1981 ctgtggaaca cccagataag ctggaggaga agttccctca ggtcgcagca actggcgacg
     2041 gtccagacat catcttctgg gcacacgata ggttcggtgg ctatgcccaa tctggcctgc
     2101 tggctgagat tactcctgat aaggctttcc aggacaagct gtatccattc acctgggatg
     2161 ctgtgagata caatggcaag ctgatcgcat accctattgc tgtggaagct ctgagcctga
     2221 tctacaacaa ggacctgctg cctaatcctc ctaagacatg ggaagaaatc cctgcactgg
     2281 acaaggaact gaaggccaaa ggcaagtccg cactgatgtt caacctgcag gagccttact
     2341 ttacctggcc actgattgct gccgacggtg gttacgcttt caagtacgag aatggtaagt
     2401 acgacatcaa agatgtgggt gtggacaatg ctggtgccaa agctggtctg actttcctgg
     2461 tggatctgat caagaacaag cacatgaatg cagacactga ctattctatc gcagaggctg
     2521 ctttcaacaa aggcgaaacc gcaatgacta tcaatggtcc ttgggcatgg tctaacatcg
     2581 acactagcaa agtcaactac ggtgtcaccg ttctgccaac cttcaagggt cagccaagca
     2641 aacctttcgt tggcgtgctg agcgcaggta tcaacgctgc ctctcctaac aaagagctgg
     2701 ctaaggagtt tctggagaac tacctgctga cagacgaagg tctggaggca gtgaacaagg
     2761 acaagccact gggtgccgtg gctctgaaga gctacgaaga agaactggcc aaggaccctc
     2821 gcatcgctgc tacaatggag aacgcacaga agggtgagat catgccaaac atcccacaga
     2881 tgagcgcatt ctggtatgcc gtgaggaccg ctgttatcaa cgcagcttct ggcagacaga
     2941 ccgtggatga agccctgaaa gacgcacaga cctaccctta cgatgtgcca gactacgcct
     3001 aagcggccgc tcataaagct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
     3061 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     3121 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     3181 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtccg
     3241 gactgtacaa ttgcattcat tttatgtttc aggttcaggg ggagatgtgg gaggtttttt
     3301 aaagcaagta aaacctctac aaatgtggta aaatcgataa ggatcttcct agagcatggc
     3361 tacgtagata agtagcatgg cgggttaatc attaactaca aggaacccct agtgatggag
     3421 ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc
     3481 cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ctgcattaat
     3541 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     3601 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     3661 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     3721 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     3781 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     3841 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     3901 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     3961 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     4021 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     4081 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     4141 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     4201 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     4261 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     4321 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     4381 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     4441 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     4501 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     4561 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     4621 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgcgac ccacgctcac
     4681 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     4741 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     4801 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     4861 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     4921 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     4981 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     5041 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     5101 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     5161 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     5221 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     5281 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     5341 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     5401 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     5461 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctaaat
     5521 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
     5581 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
     5641 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
     5701 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
     5761 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
     5821 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
     5881 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
     5941 cgccgcgctt aatgcgccgc tacagggcgc gt
//
