LOCUS       sequence-501930-        6506 bp    DNA     circular SYN 04-MAR-2026
DEFINITION  Tight affinity glutamate sensor ** A newer version of this sensor is
            available. Please see iGluSnFR3 plasmids at 
            https://www.addgene.org/browse/article/28220233/ **.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6506)
  AUTHORS   Marvin JS, Scholl B, Wilson DE, Podgorski K, Kazemipour A, Muller 
            JA, Schoch S, Quiroz FJU, Rebola N, Bao H, Little JP, Tkachuk AN, 
            Cai E, Hantman AW, Wang SS, DePiero VJ, Borghuis BG, Chapman ER, 
            Dietrich D, DiGregorio DA, Fitzpatrick D, Looger LL
  TITLE     Stability, affinity, and chromatic variants of the glutamate sensor 
            iGluSnFR.
  JOURNAL   Nat Methods. 2018 Nov;15(11):936-939. doi: 
            10.1038/s41592-018-0171-3. Epub 2018 Oct 30.
  PUBMED    30377363
REFERENCE   2  (bases 1 to 6506)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 4, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-018-0171-3"; journalName: "Nat Methods"; date: 
            "2018-11"; volume: "15"; issue: "11"; pages: "936-939"
COMMENT     Sequence Label: pAAV.CAG.SF-iGluSnFR.A184S
FEATURES             Location/Qualifiers
     source          1..6506
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    1095..1216
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1132..1151)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1186..1205
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     misc_feature    1259..1847
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             1365..1376
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     1774..1794
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1863..2009)
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     CDS             complement(2013..2042)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     2507..2528
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2631..2652)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     sig_peptide     complement(3621..3680)
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     regulatory      3677..3686
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(3720..3739)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(3801..3823)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     promoter        complement(4102..4378)
                     /label=chicken beta-actin promoter
     repeat_region   4689..4818
                     /label=AAV2 ITR
     rep_origin      4890..5345
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4977..4996)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5187..5208
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5371..5475
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5476..5544,5545..6336)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5694..5713)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctgcg cgctcgctcg
      841 ctcactgagg ccgcccgggc aaagcccggg cggcctcagt gagcgagcga gcgcgcagag
      901 agggagtggc caactccatc actaggggtt ccttgtagtt aatgattaac ccgccatgct
      961 acttatctac gtagccatgc tctaggaaga tccttatcga ttttaccaca tttgtagagg
     1021 ttttacttgc tttaaaaaac ctcccacatc tccccctgaa cctgaaacat aaaatgaatg
     1081 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     1141 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     1201 tcatcaatgt atcttatcat gtctgctcga agcggccgcc cgggtcgacg gtatcgatgc
     1261 ggggaggcgg cccaaaggga gatccgactc gtctgagggc gaaggcgaag acgcggaaga
     1321 ggccgcagag ccggcagcag gccgcgggaa ggaaggtccg ctggattgag ggccgaaggg
     1381 acgtagcaga aggacgtccc gcgcagaatc caggtggcaa cacaggcgag cagccaagga
     1441 aaggacgatg atttccccga caacaccacg gaattgtcag tgcccaacag ccgagcccct
     1501 gtccagcagc gggcaaggca ggcggcgatg agttccgccg tggcaatagg gagggggaaa
     1561 gcgaaagtcc cggaaaggag ctgacaggtg gtggcaatgc cccaaccagt gggggttgcg
     1621 tcagcaaaca cagtgcacac cacgccacgt tgcctgacaa cgggccacaa ctcctcataa
     1681 agagacagca accaggattt atacaaggag gagaaaatga aagccatacg ggaagcaata
     1741 gcatgataca aaggcattaa agcagcgtat ccacatagcg taaaaggagc aacatagtta
     1801 agaataccag tcaatctttc acaaattttg taatccagag gttgattatc gataagcttc
     1861 taacgtggct tcttctgcca aagcatgatg aggatgataa gggagatgat ggtgagcacc
     1921 accagggcca ggatggctga gatcaccacc accttaaagg gcaaggagtg tggcaccacg
     1981 atgacctcct gcgtgtcctg gcccacagca ttcagatcct cttctgagat gagtttttgt
     2041 tcgtcgacct gcagtttcag tgccttgtca ttcggttctt tgaacagtgc tttcatttcg
     2101 tctgacagtt cgaaattcat gttcaggggg ttgttaaagt tgtactccag cttgtgcccc
     2161 aggatgttgc cgtcctcctt gaagtcgatg cccttcagct cgatgcggtt caccagggtg
     2221 tcgccctcga acttcacctc ggcgcgggtc ttgtaggtgc cgtcgtcctt gaagctgatg
     2281 gtgcgctcct ggacgtagcc ttcgggcatg gcggacttga agaagtcgtg ctgcttcatg
     2341 tggtcggggt agcggctgaa gcactgcacg ccgtaggtca gggtggtcac gagggtgggc
     2401 cagggcacgg gcagcttgcc ggtggtgcag atgaacttca gggtcagctt gccgttggtg
     2461 gcatcgccct cgccctcgcc gcgcacgctg aacttgtggc cgtttacgtc gccgtccagc
     2521 tcgaccagga tgggcaccac cccggtgaac agctcctcgc ccttgctcat gctccctccg
     2581 gtaccgccct tgtacagctc gtccatgccg agagtgatcc cggcggcggt cacgaactcc
     2641 agcaggacca tgtgatcgcg cttctcgttg gggtctttgc tcagcacgga ctgggtgctc
     2701 aggtagtggt tgtcgggcag cagcacgggg ccgtcgccga tgggggtgtt ctgctggtag
     2761 tggtcggcga gctgcacgct gccgtcctcc acgttgtggc ggatcttgaa gttcgccttg
     2821 atgccgttct tctgcttgtc ggcggtgata tagacgttgt ggctcaccag aattggattt
     2881 ttgaaccact tatcaaacca tttttccgct tcaccggagg tctgcacctg agcgatggtg
     2941 tcatccatca gctttttgaa ctgcggatca tctttacgca acatacaacc gtaggcctcc
     3001 tgagactgcg gcttgccgac gatttcccag ttgtctggtt tcttcgcttt cgcacgttca
     3061 ccggccagca ggctgtcatc catcataaag gcaacggcac gaccgctttc cagggtgcgg
     3121 aaagagtcac cgtgatcttt ggcgctgatg atgcgcatat tcattttttg ctcttcattc
     3181 agtttgttga gcaaaacttc agaggtagtg ccggaagtga cgactacggc tttgtctttc
     3241 aggttggcaa aatctttgat atcgccaccc tttttggtca acaggcgcgt accgaccacg
     3301 aaaatagtgt cagagaaagc cgcctgtttt tggcgttcga cgttgttggt ggtagaacca
     3361 cattcaaaat cgaaagtgcc gttttgcagc agtggaatac ggttttgtga ggtaatcgga
     3421 atcagtttta cctgcaagtc cggtttgttg agtttctttt tcactgcttc aacaatggcg
     3481 ttggagtaat cctgcgagta acccaccact ttttgctgat tgtcgtaata agagaaaggc
     3541 actgaagatt cacggtgacc gacgacaatc acaccgtttt tggcgatttt gtccagcgtg
     3601 ctgcctgcgg cagatctgtc accagtggaa cctggaaccc agagcagcag tacccatagc
     3661 aggagtgtgt ctgtctccat ggtggcggga tccaattctt tgccaaaatg atgagacagc
     3721 acaataacca gcacgttgcc caggagctgt aggaaaaaga agaaggcatg aacatggtta
     3781 gcagaggctc tagagccgcc ggtcacacgc cagaagccga accccgccct gccccgtccc
     3841 ccccgaaggc agccgtcccc ccgcggacag ccccgagccg ctccccccgc acaaagggcc
     3901 ctcccggagc ccctcaaggc tttcacgcag ccacagaaaa gaaacaagcc gtcattaaac
     3961 caagcgctaa ttacagcccg gaggagaagg gccgtcccgc ccgctcacct gtgggagtaa
     4021 cgcggtcagt cagagccggg gcgggcggcg cgaggcggcg gcggagcggg gcacggggcg
     4081 aaggcagcgc gcagcgactc ccgcccgccg cgcgcttcgc tttttatagg gccgccgccg
     4141 ccgccgcctc gccataaaag gaaactttcg gagcgcgccg ctctgattgg ctgccgccgc
     4201 acctctccgc ctcgccccgc cccgcccctc gccccgcccc gccccgcctg gcgcgcgccc
     4261 cccccccccc ccgcccccat cgctgcacaa aataattaaa aaataaataa atacaaaatt
     4321 gggggtgggg agggggggga gatggggaga gtgaagcaga acgtggggct cacctcgacc
     4381 atggtaatag cgatgactaa tacgtagatg tactgccaag taggaaagtc ccataaggtc
     4441 atgtactggg cataatgcca ggcgggccat ttaccgtcat tgacgtcaat agggggcgta
     4501 cttggcatat gatacacttg atgtactgcc aagtgggcag tttaccgtaa atactccacc
     4561 cattgacgtc aatggaaagt ccctattggc gttactatgg gaacatacgt cattattgac
     4621 gtcaatggta ctcttcctag agcatggcta cgtagataag tagcatggcg ggttaatcat
     4681 taactacaag gaacccctag tgatggagtt ggccactccc tctctgcgcg ctcgctcgct
     4741 cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg cggcctcagt
     4801 gagcgagcga gcgcgcagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     4861 acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc
     4921 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     4981 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     5041 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     5101 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     5161 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     5221 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     5281 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac
     5341 aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     5401 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     5461 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     5521 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     5581 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     5641 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     5701 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     5761 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     5821 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     5881 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     5941 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     6001 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     6061 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     6121 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     6181 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     6241 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     6301 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     6361 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     6421 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     6481 cccgtagaaa agatcaaagg atcttc
//
