LOCUS       sequence-502001-        3463 bp    DNA     circular SYN 26-MAR-2026
DEFINITION  Hs TRAC and TRBC gene for makeTCR assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3463)
  TITLE     makeTCR collection
REFERENCE   2  (bases 1 to 3463)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 26, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: makeTCR_Hs.TRxC.puro
FEATURES             Location/Qualifiers
     source          1..3463
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             560..613
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             1097..1156
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     primer_bind     complement(1793..1809)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1793..1809)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      1848..1879
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        1880..1982
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1983..2642
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2049..2068)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      2666..2693
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(2705..3292)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2785..2804)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(3314..3343)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     primer_bind     3416..3433
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3417..3433
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cgtctcagac ctgaacaagg tgttcccacc cgaggtcgct gtgtttgagc catcagaagc
       61 agagatctcc cacacccaaa aggccacact ggtgtgcctg gccacaggct tcttccctga
      121 ccacgtggag ctgagctggt gggtgaatgg gaaggaggtg cacagtgggg tcagcacgga
      181 cccgcagccc ctcaaggagc agcccgccct caatgactcc agatactgcc tgagcagccg
      241 cctgagagtg tccgccacct tctggcagaa cccccgcaac cacttccgct gtcaagtcca
      301 gttctacggg ctctcggaga atgacgagtg gacccaggat agggccaaac ccgtcaccca
      361 gatcgtcagc gccgaggcct ggggtagagc agactgtggc tttacctcgg tgtcctacca
      421 gcaaggggtc ctgtctgcca ccatcctcta tgagatcctg ctagggaagg ccaccctgta
      481 tgctgtgctg gtcagcgccc ttgtgttgat ggccatggtc aagagaaagg atttcagccg
      541 gcggaagagg ggaagcggag agggcagggg aagtcttcta acatgcgggg acgtggagga
      601 aaatcccggc cccgggatgg gagacggagg ggcagtggaa cgaggacccg tctcgatcca
      661 gaaccctgac cctgccgtgt accagctgag agactctaaa tccagtgaca agtctgtctg
      721 cctattcacc gattttgatt ctcaaacaaa tgtgtcacaa agtaaggatt ctgatgtgta
      781 tatcacagac aaaactgtgc tagacatgag gtctatggac ttcaagagca acagtgctgt
      841 ggcctggagc aacaaatctg actttgcatg tgcaaacgcc ttcaacaaca gcattattcc
      901 agaagacacc ttcttcccca gcccagaaag ttcctgtgat gtcaagctgg tcgagaaaag
      961 ctttgaaaca gatacgaacc taaactttca aaacctgtca gtgattgggt tccgaatcct
     1021 cctcctgaaa gtggccgggt ttaatctgct catgacgctg cggctgtggt ccagccggaa
     1081 gcggagggga agcggacagt gtactaatta tgctctcttg aaattggctg gagatgttga
     1141 gagcaaccca ggtcccatgg tcactgaata taaaccaacc gttagactgg caacacggga
     1201 tgatgtgcat cgggcagtga gaactctggc tgctgcattt gcagattatc ctgctacacg
     1261 gcatacagtt gacccagata ggcatattga aagagttaca gaactccagg agcttttcct
     1321 taccagagtt ggcttggata ttggaaaagt ttgggtggcc gatgatgggg cagccgtcgc
     1381 tgtgtggacg acccccgaat ccgtagaggc aggagctgtt ttcgcagaaa ttgggccccg
     1441 tatggctgaa ctctcaggga gccgcctcgc ggcccaacag caaatggaag gactgctcgc
     1501 acctcataga ccgaaagaac cggcttggtt tcttgcgaca gtcggggtga gcccagatca
     1561 tcaaggaaaa ggactcgggt cagcagtagt cctgcctggg gtagaagccg ctgaaagagc
     1621 aggcgttcca gcttttcttg agacatctgc cccacgtaat cttccatttt atgaacgcct
     1681 gggttttaca gtgacagcag atgtggaagt tccagagggc caaagaacgt ggtgtatgac
     1741 acggaaaccg ggcgcgtaac tgagacgcag ggaagatggc cgcgaagaaa acgtcatagc
     1801 tgtttcctga ctgaccattt aaatcatacc tgacctccat agcagaaagt caaaagcctc
     1861 cgaccggagg cttttgactt gatcggcacg taagaggttc caactttcac cataatgaaa
     1921 taagatcact accgggcgta ttttttgagt tatcgagatt ttcaggagct aaggaagcta
     1981 aaatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg catcgtaaag
     2041 aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc gttcagctgg
     2101 atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat ccggccttta
     2161 ttcacattct tgcccgcctg atgaatgctc atccggaatt tcgtatggca atgaaagacg
     2221 gtgagctggt gatatgggat agtgttcacc cttgttacac cgttttccat gagcaaactg
     2281 aaacgttttc atcgctctgg agtgaatacc acgacgattt ccggcagttt ctacacatat
     2341 attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa gggtttattg
     2401 agaatatgtt ttttgtctca gccaatccct gggtgagttt caccagtttt gatttaaacg
     2461 tggccaatat ggacaacttc ttcgcccccg ttttcaccat gggcaaatat tatacgcaag
     2521 gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtttgt gatggcttcc
     2581 atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag ggcggggcgt
     2641 aatgagggcc caaatgtaat cacctggctc accttcgggt gggcctttct gcgttgctgg
     2701 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgatgc tcaagtcaga
     2761 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     2821 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     2881 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     2941 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     3001 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     3061 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     3121 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     3181 ttacctcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     3241 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     3301 tttgattttc taccgaagaa aggcccaccc gtgaaggtga gccagtgagt tgattgcagt
     3361 ccagttacgc tggagtctga ggctcgtcct gaatgatatc aagcttgaat tcgtttgtaa
     3421 aacgacggcc agtgctaacc gtatcgcatt ggccatacct aca
//
