LOCUS       sequence-502005-        2756 bp    DNA     circular SYN 26-MAR-2026
DEFINITION  MmTRAC and TRBC gene for makeTCR assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2756)
  TITLE     makeTCR collection
REFERENCE   2  (bases 1 to 2756)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 26, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: makeTCR_Mm.TRxC.dis
FEATURES             Location/Qualifiers
     source          1..2756
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             545..598
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(1086..1102)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1086..1102)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      1141..1172
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        1173..1275
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1276..1935
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1342..1361)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1959..1986
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1998..2585)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2078..2097)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2607..2636)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     primer_bind     2709..2726
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2710..2726
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cgtctcagac ctgagaaatg tgactccacc caaggtttcc ttgtttgagc catcaaaagc
       61 agagattgca aacaaacaaa aggctaccct cgtgtgcttg gccaggggct tcttccctga
      121 ccacgtggag ctgagctggt gggtgaatgg caaggaggtc cacagtgggg tctgcacgga
      181 ccctcaggcc tacaaggaga gcaattatag ctactgcctg agcagccgcc tgagggtttc
      241 tgctaccttc tggcacaatc ctcgcaacca cttccgctgc caagtgcagt tccatgggct
      301 ttcagaggag gacaagtggc cagagggctc acccaaacct gtcacacaga acatcagtgc
      361 agaggcctgg ggccgagcag actgtgggat tacctcagca tcctatcaac aaggggtctt
      421 gtctgccacc atcctctatg agatcctgct agggaaagcc accctgtatg ctgtgcttgt
      481 cagtacactg gtggtgatgg ctatggtcaa aagaaagaat tcacggcgga agaggggaag
      541 cggagagggc aggggaagtc ttctaacatg cggggacgtg gaggaaaatc ccggccccgg
      601 gatgggagac ggaggggcag tggaacgagg acccgtctcg atccagaacc cagaacctgc
      661 tgtgtaccag ttaaaagatc ctcggagcca ggacagcacc ctctgcctgt tcaccgactt
      721 tgactcccaa atcaatgtgc cgaaaaccat ggaatctgga acgttcatca ctgacaaatg
      781 tgtgctggac atgaaagcta tggattccaa gagcaatggg gccattgcct ggagcaacca
      841 gacaagcttc acgtgccaag atatcttcaa agaaaccaac gccacctacc ccagttcaga
      901 cgttccctgt gatgccacgt tgactgagaa aagctttgaa acagatatga acctaaactt
      961 tcaaaacctg tcagttatgg gactccgaat cctcctgctg aaagtagccg gatttaacct
     1021 gctcatgacg ctgaggctgt ggtccagcta actgagacga cagggaagat ggccgcgaag
     1081 aaaacgtcat agctgtttcc tgactgacca tttaaatcat acctgacctc catagcagaa
     1141 agtcaaaagc ctccgaccgg aggcttttga cttgatcggc acgtaagagg ttccaacttt
     1201 caccataatg aaataagatc actaccgggc gtattttttg agttatcgag attttcagga
     1261 gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa
     1321 tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag
     1381 accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt
     1441 tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga atttcgtatg
     1501 gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc
     1561 catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag
     1621 tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct
     1681 aaagggttta ttgagaatat gttttttgtc tcagccaatc cctgggtgag tttcaccagt
     1741 tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac catgggcaaa
     1801 tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt
     1861 tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg cgatgagtgg
     1921 cagggcgggg cgtaatgagg gcccaaatgt aatcacctgg ctcaccttcg ggtgggcctt
     1981 tctgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     2041 tgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     2101 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     2161 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     2221 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     2281 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     2341 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     2401 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     2461 ctgctgaagc cagttacctc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     2521 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     2581 ctcaagaaga tcctttgatt ttctaccgaa gaaaggccca cccgtgaagg tgagccagtg
     2641 agttgattgc agtccagtta cgctggagtc tgaggctcgt cctgaatgat atcaagcttg
     2701 aattcgtttg taaaacgacg gccagtgcta accgtatcgc attggccata cctaca
//
