LOCUS       sequence-502155-       12499 bp    DNA     circular SYN 09-MAR-2026
DEFINITION  Editing Photene desaturase (PDS) genes in Nicotiana benthamiana.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12499)
  AUTHORS   Ugrappa N, Rodriguez JE, Nguyen T, Weissman RF, Thornton BW, Terrace
            CI, Savage DF, Dinesh-Kumar SP
  TITLE     High-efficiency, transgene-free plant genome editing by viral 
            delivery of an engineered TnpB
  JOURNAL   Nat. Plants (2026). 20 February 2026.
REFERENCE   2  (bases 1 to 12499)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 9, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat. Plants
            (2026). 20 February 2026."
COMMENT     Sequence Label: 4927 TRV2-TnpB-reRNA4PDS
FEATURES             Location/Qualifiers
     source          1..12499
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        427..772
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     715..735
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     ncRNA           3823..3890
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
     CDS             4429..4446
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      4481..4733
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    4755..4779
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6080..6709
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7138..8211
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      8277..8471
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(8707..8729)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    8815..8955
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     8870..8889
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8970..8987)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9141..9729)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9221..9240)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(11154..11948)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    12373..12397
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 aagcttgcat gcctgcaggt caacatggtg gagcacgaca ctctcgtcta ctccaagaat
       61 atcaaagata cagtctcaga agaccagagg gctattgaga cttttcaaca aagggtaata
      121 tcgggaaacc tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta
      181 gaaaaggaag atggcttcta caaatgccat cattgcgata aaggaaaggc tatcgttcaa
      241 gatgcctcta ccgacagtgg tcccaaagat ggacccccac ccacgaggaa catcgtggaa
      301 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgatgg tcaacatggt
      361 ggagcacgac actctcgtct actccaagaa tatcaaagat acagtctcag aagaccagag
      421 ggctattgag acttttcaac aaagggtaat atcgggaaac ctcctcggat tccattgccc
      481 agctatctgt cacttcatcg aaaggacagt agaaaaggaa gatggcttct acaaatgcca
      541 tcattgcgat aaaggaaagg ctatcgttca agatgcctct accgacagtg gtcccaaaga
      601 tggaccccca cccacgagga acatcgtgga aaaagaagac gttccaacca cgtcttcaaa
      661 gcaagtggat tgatgtgata tctccactga cgtaagggat gacgcacaat cccactatcc
      721 ttcgcaagac ccttcctcta tataaggaag ttcatttcat ttggagagga taaaacattg
      781 cacctatggt gttgccctgg ctggggtatg tcagtgatcg cagtagaatg tactaattga
      841 caagttggag aatacggtag aacgtcctta tccaacacag cctttatccc tctccctgac
      901 gaggtttttg tcagtgtaat atttcttttt gaactatcca gcttagtacc gtacgggaaa
      961 gtgactggtg tgcttatctt tgaaatgtta ctttgggttt cggttcttta ggttagtaag
     1021 aaagcacttg tcttctcata caaaggaaaa cctgagacgt atcgcttacg aaagtagcaa
     1081 tgaaagaaag gtggtggttt taatcgctac cgcaaaaacg atggggtcgt tttaattaac
     1141 ttctcctacg caagcgtcta aacggacgtt ggggttttgc tagtttcttt agagaaaact
     1201 agctaagtct ttaatgttat cattagagat ggcataaata taatacttgt gtctgctgat
     1261 aagatcattt taatttggac gattagactt gttgaactac aggttactga atcacttgcg
     1321 ctaatcaaca tgggagatat gtacgatgaa tcatttgaca agtcgggcgg tcctgctgac
     1381 ttgatggacg attcttgggt ggaatcagtt tcgtggaaag atctgttgaa gaagttacac
     1441 agcataaaat ttgcactaca gtctggtaga gatgagatca ctgggttact agcggcactg
     1501 aatagacagt gtccttattc accatatgag cagtttccag ataagaaggt gtatttcctt
     1561 ttagactcac gggctaacag tgctcttggt gtgattcaga acgcttcagc gttcaagaga
     1621 cgagctgatg agaagaatgc agtggcgggt gttacaaata ttcctgcgaa tccaaacaca
     1681 acggttacga cgaaccaagg gagtactact actaccaagg cgaacactgg ctcgactttg
     1741 gaagaagact tgtacactta ttacaaattc gatgatgcct ctacagcttt ccacaaatct
     1801 ctaacttcgt tagagaacat ggagttgaag agttattacc gaaggaactt tgagaaagta
     1861 ttcgggatta agtttggtgg agcagctgct agttcatctg caccgcctcc agcgagtgga
     1921 ggtccgatac gtcctaatcc ctagggattt aaggacgtga actctgttga gatctctgtg
     1981 aaattcagag ggtgggtgat accatattca ctgatgccat tagcgacatc taaatagggc
     2041 taattgtgac taatttgagg gaatttcctt taccattgac gtcagtgtcg ttggtagcat
     2101 ttgagtttcg gcacacaagg ttaaaaacgc tgtagtaata catgcgcaag aacaggctga
     2161 gcatcttgtt ctggggtttc acactatctt tagagaaagt gttaagttaa ttaagttatc
     2221 ttaattaaga gcataattat actgatttgt ctctcgttga tagagtctat cattctgtta
     2281 ttaaaaattt gacaactcgg tttgctgacc tactggtcac tgtatcactt acccgagtta
     2341 acgagtctag aatgatacgc aataaggcat ttgtggttcg gctctacccg aatgcggcac
     2401 aaactgagct gataaaccga acactggggt ctgcacgatt tgtatacaat catttccttg
     2461 ctcgaagaat cgctgcgtac aaggaaagcg gtaagggtct tacgtacggg caaacttcta
     2521 gtgagcttac tctgctgaag caagcagagg aaacctcctg gctgtcagaa gtggataaat
     2581 tcgcgttgca gaatagcttg aaaaaccttg aaacggccta taaaaacttc tttagaacag
     2641 ttaagcagtc aggaaaaaag gtaggtttcc ctcggttcag gaagaagcgc acgggggagt
     2701 cataccgcac gcagtttacg aacaacaaca ttcagatagg agaggggagg cttaaactgc
     2761 cgaaactggg gtgggtcaaa actaagggtc agcaggatat ccaaggcaaa atattgaatg
     2821 ttactgtacg ccgcatccat gaaggccatt atgaagcttc agtgctgtgc gaggtcgaaa
     2881 tcccttacct cccagctgct ccgaaattcg cggcaggagt agacgtgggg attaaagact
     2941 ttgcgatcgt caccgatggt gtgcgattta agcatgagca aaatcctaaa tactatcgga
     3001 gtacgcttaa gcgactccgg aaggcccaac agactctgtc caggagaaag aaaggaagcg
     3061 ctagatatgg caaggctaag actaagctgg caaggattca caagcgcatc gtgaataaga
     3121 gacaagattt tctgcacaaa ctgaccactt cccttgtgcg cgagtatgaa ataatcggga
     3181 ctgaacacct gaagcccgac aatatgcgca agaatagaag gctggcgctc agcatctcag
     3241 atgctggatg gggtgaattc ataaggcagt tggagtacaa agctgcttgg tatgggagac
     3301 tcgtaagtaa agtttcccca tattttcctt ctagtcaact ctgtcacgac tgcggattca
     3361 aaaaccccga agtgaaaaat ctcgcggtaa ggacttggac gtgcccaaac tgtggcgaga
     3421 cacacgacag agatgaaaat gcggcgctga atatcaggag agaggcgttg gtggctgcgg
     3481 ggatttcaga tacccttaac gcgcacggcg gttacgtgcg acccgcttcc gccggtaacg
     3541 gccttaggag tgaaaaccac gctactctcg tagtgagcag ggccgacgaa ggttcaggga
     3601 atagaagaca taagcgtatg atcaagaaca gagaatctgc agctcgttcc cgcgctagga
     3661 aacaggctta tacaaactag ggtaccggtg gctgcgggaa tctcagacac cttaaacgct
     3721 catggaggct atgtcagacc tgcttcggcg ggcaatggtc tgcgaagtga gaatcacgcg
     3781 actttagtcg tgtgaggttc aagctacaat gaaggaacta gcggccggca tggtcccagc
     3841 ctcctcgctg gcgccggctg ggcaacatgc ttcggcatgg cgaatgggac gagctcccgt
     3901 agctcagttg gttagagcgt tggtcttatg agccgaaggt cgcgggttcg agccccgccg
     3961 gaagcacccg ggcatgtccc gaagacatta aactacggtt ctttaagtag atccgtgtct
     4021 gaagttttag gttcaattta aacctacgag attgacattc tcgactgatc ttgattgatc
     4081 ggtaagtctt ttgtaattta attttctttt tgattttatt ttaaattgtt atctgtttct
     4141 gtgtatagac tgtttgagat cggcgtttgg ccgactcatt gtcttaccat aggggaacgg
     4201 actttgtttg tgttgttatt ttatttgtat tttattaaaa ttctcaacga tctgaaaaag
     4261 cctcgcggct aagagattgt tggggggtga gtaagtactt ttaaagtgat gatggttaca
     4321 aaggcaaaag gggtaaaacc cctcgcctac gtaagcgtta ttacgcccgt ctgtacttat
     4381 atcagtacac tgacgagtcc ctaaaggacg aaacgggaga acgctagcca ccaccaccac
     4441 caccacgtgt gaattacagg tgaccagctc gaatttcccc gatcgttcaa acatttggca
     4501 ataaagtttc ttaagattga atcctgttgc cggtcttgcg atgattatca tataatttct
     4561 gttgaattac gttaagcatg taataattaa catgtaatgc atgacgttat ttatgagatg
     4621 ggtttttatg attagagtcc cgcaattata catttaatac gcgatagaaa acaaaatata
     4681 gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct atgttactag atcgggaatt
     4741 aaactatcag tgtttgacag gatatattgg cgggtaaacc taagagaaaa gagcgtttat
     4801 tagaataatc ggatatttaa aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt
     4861 gcatgccaac cacagggttc ccctcgggat caaagtactt tgatccaacc cctccgctgc
     4921 tatagtgcag tcggcttctg acgttcagtg cagccgtctt ctgaaaacga catgtcgcac
     4981 aagtcctaag ttacgcgaca ggctgccgcc ctgccctttt cctggcgttt tcttgtcgcg
     5041 tgttttagtc gcataaagta gaatacttgc gactagaacc ggagacatta cgccatgaac
     5101 aagagcgccg ccgctggcct gctgggctat gcccgcgtca gcaccgacga ccaggacttg
     5161 accaaccaac gggccgaact gcacgcggcc ggctgcacca agctgttttc cgagaagatc
     5221 accggcacca ggcgcgaccg cccggagctg gccaggatgc ttgaccacct acgccctggc
     5281 gacgttgtga cagtgaccag gctagaccgc ctggcccgca gcacccgcga cctactggac
     5341 attgccgagc gcatccagga ggccggcgcg ggcctgcgta gcctggcaga gccgtgggcc
     5401 gacaccacca cgccggccgg ccgcatggtg ttgaccgtgt tcgccggcat tgccgagttc
     5461 gagcgttccc taatcatcga ccgcacccgg agcgggcgcg aggccgccaa ggcccgaggc
     5521 gtgaagtttg gcccccgccc taccctcacc ccggcacaga tcgcgcacgc ccgcgagctg
     5581 atcgaccagg aaggccgcac cgtgaaagag gcggctgcac tgcttggcgt gcatcgctcg
     5641 accctgtacc gcgcacttga gcgcagcgag gaagtgacgc ccaccgaggc caggcggcgc
     5701 ggtgccttcc gtgaggacgc attgaccgag gccgacgccc tggcggccgc cgagaatgaa
     5761 cgccaagagg aacaagcatg aaaccgcacc aggacggcca ggacgaaccg tttttcatta
     5821 ccgaagagat cgaggcggag atgatcgcgg ccgggtacgt gttcgagccg cccgcgcacg
     5881 tctcaaccgt gcggctgcat gaaatcctgg ccggtttgtc tgatgccaag ctggcggcct
     5941 ggccggccag cttggccgct gaagaaaccg agcgccgccg tctaaaaagg tgatgtgtat
     6001 ttgagtaaaa cagcttgcgt catgcggtcg ctgcgtatat gatgcgatga gtaaataaac
     6061 aaatacgcaa ggggaacgca tgaaggttat cgctgtactt aaccagaaag gcgggtcagg
     6121 caagacgacc atcgcaaccc atctagcccg cgccctgcaa ctcgccgggg ccgatgttct
     6181 gttagtcgat tccgatcccc agggcagtgc ccgcgattgg gcggccgtgc gggaagatca
     6241 accgctaacc gttgtcggca tcgaccgccc gacgattgac cgcgacgtga aggccatcgg
     6301 ccggcgcgac ttcgtagtga tcgacggagc gccccaggcg gcggacttgg ctgtgtccgc
     6361 gatcaaggca gccgacttcg tgctgattcc ggtgcagcca agcccttacg acatatgggc
     6421 caccgccgac ctggtggagc tggttaagca gcgcattgag gtcacggatg gaaggctaca
     6481 agcggccttt gtcgtgtcgc gggcgatcaa aggcacgcgc atcggcggtg aggttgccga
     6541 ggcgctggcc gggtacgagc tgcccattct tgagtcccgt atcacgcagc gcgtgagcta
     6601 cccaggcact gccgccgccg gcacaaccgt tcttgaatca gaacccgagg gcgacgctgc
     6661 ccgcgaggtc caggcgctgg ccgctgaaat taaatcaaaa ctcatttgag ttaatgaggt
     6721 aaagagaaaa tgagcaaaag cacaaacacg ctaagtgccg gccgtccgag cgcacgcagc
     6781 agcaaggctg caacgttggc cagcctggca gacacgccag ccatgaagcg ggtcaacttt
     6841 cagttgccgg cggaggatca caccaagctg aagatgtacg cggtacgcca aggcaagacc
     6901 attaccgagc tgctatctga atacatcgcg cagctaccag agtaaatgag caaatgaata
     6961 aatgagtaga tgaattttag cggctaaagg aggcggcatg gaaaatcaag aacaaccagg
     7021 caccgacgcc gtggaatgcc ccatgtgtgg aggaacgggc ggttggccag gcgtaagcgg
     7081 ctgggttgcc tgccggccct gcaatggcac tggaaccccc aagcccgagg aatcggcgtg
     7141 agcggtcgca aaccatccgg cccggtacaa atcggcgcgg cgctgggtga tgacctggtg
     7201 gagaagttga aggccgcgca ggccgcccag cggcaacgca tcgaggcaga agcacgcccc
     7261 ggtgaatcgt ggcaagcggc cgctgatcga atccgcaaag aatcccggca accgccggca
     7321 gccggtgcgc cgtcgattag gaagccgccc aagggcgacg agcaaccaga ttttttcgtt
     7381 ccgatgctct atgacgtggg cacccgcgat agtcgcagca tcatggacgt ggccgttttc
     7441 cgtctgtcga agcgtgaccg acgagctggc gaggtgatcc gctacgagct tccagacggg
     7501 cacgtagagg tttccgcagg gccggccggc atggccagtg tgtgggatta cgacctggta
     7561 ctgatggcgg tttcccatct aaccgaatcc atgaaccgat accgggaagg gaagggagac
     7621 aagcccggcc gcgtgttccg tccacacgtt gcggacgtac tcaagttctg ccggcgagcc
     7681 gatggcggaa agcagaaaga cgacctggta gaaacctgca ttcggttaaa caccacgcac
     7741 gttgccatgc agcgtacgaa gaaggccaag aacggccgcc tggtgacggt atccgagggt
     7801 gaagccttga ttagccgcta caagatcgta aagagcgaaa ccgggcggcc ggagtacatc
     7861 gagatcgagc tagctgattg gatgtaccgc gagatcacag aaggcaagaa cccggacgtg
     7921 ctgacggttc accccgatta ctttttgatc gatcccggca tcggccgttt tctctaccgc
     7981 ctggcacgcc gcgccgcagg caaggcagaa gccagatggt tgttcaagac gatctacgaa
     8041 cgcagtggca gcgccggaga gttcaagaag ttctgtttca ccgtgcgcaa gctgatcggg
     8101 tcaaatgacc tgccggagta cgatttgaag gaggaggcgg ggcaggctgg cccgatccta
     8161 gtcatgcgct accgcaacct gatcgagggc gaagcatccg ccggttccta atgtacggag
     8221 cagatgctag ggcaaattgc cctagcaggg gaaaaaggtc gaaaaggtct ctttcctgtg
     8281 gatagcacgt acattgggaa cccaaagccg tacattggga accggaaccc gtacattggg
     8341 aacccaaagc cgtacattgg gaaccggtca cacatgtaag tgactgatat aaaagagaaa
     8401 aaaggcgatt tttccgccta aaactcttta aaacttatta aaactcttaa aacccgcctg
     8461 gcctgtgcat aactgtctgg ccagcgcaca gccgaagagc tgcaaaaagc gcctaccctt
     8521 cggtcgctgc gctccctacg ccccgccgct tcgcgtcggc ctatcgcggc cgctggccgc
     8581 tcaaaaatgg ctggcctacg gccaggcaat ctaccagggc gcggacaagc cgcgccgtcg
     8641 ccactcgacc gccggcgccc acatcaaggc accctgcctc gcgcgtttcg gtgatgacgg
     8701 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
     8761 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc
     8821 catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag
     8881 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
     8941 aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     9001 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     9061 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     9121 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     9181 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     9241 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     9301 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     9361 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     9421 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     9481 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     9541 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
     9601 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     9661 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     9721 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     9781 tcacgttaag ggctgatgaa tcccctaatg atttttatca aaatcattaa gttaaggtag
     9841 atacacatct tgtcatatga tcaaatggtt tcgccaaaaa tcaataatca gacaacaaaa
     9901 tgtgcgaact cgatatttta cacgactctc tttaccaatt ctgccccgaa ttacacttaa
     9961 aacgactcaa cagcttaacg ttggcttgcc acgccttact tgactgtaaa actctcactc
    10021 ttaccgaact tggccgtaac ctgccaacca aagcgagaac aaaacataac atcaaacgaa
    10081 tcgaccgatt gttaggtaat cgtcacctcc acaaagagcg actcgctgta taccgttggc
    10141 atgctagctt tatctgttcg ggcaatacga tgcccattgt acttgttgac tggtctgata
    10201 tccgtgagca aaaacggctt atggtattgc gagcttcagt cgcactacac ggtcgttctg
    10261 ttactcttta tgagaaagcg ttcccgcttt cagagcaatg ttcaaagaaa gctcatgacc
    10321 aatttctagc cgaccttgcg agcattctac cgagtaacac cacaccgctc attgtcagtg
    10381 atgctggctt taaagtgcca tggtataaat ccgttgagaa gctgggttgg tactggttaa
    10441 gtcgagtaag aggaaaagta caatatgcag acctaggagc ggaaaactgg aaacctatca
    10501 gcaacttaca tgatatgtca tctagtcact caaagacttt aggctataag aggctgacta
    10561 aaagcaatcc aatctcatgc caaattctat tgtataaatc tcgctctaaa ggccgaaaaa
    10621 atcagcgctc gacacggact cattgtcacc acccgtcacc taaaatctac tcagcgtcgg
    10681 caaaggagcc atggattcta gcaactaact tacctgttga aattcgaaca cccaaacaac
    10741 ttgttaatat ctattcgaag cgaatgcaga ttgaagaaac cttccgagac ttgaaaagtc
    10801 ctgcctacgg actaggccta cgccatagcc gaacgagcag ctcagagcgt tttgatatca
    10861 tgctgctaat cgccctgatg cttcaactaa catgttggct tgcgggcgtt catgctcaga
    10921 aacaaggttg ggacaagcac ttccaggcta acacagtcag aaatcgaaac gtactctcaa
    10981 cagttcgctt aggcatggaa gttttgcggc attctggcta cacaataaca agggaagact
    11041 cactcgtggc tgcaaccctg cttactcaaa atctattcac acatggttac gttttgggga
    11101 aattatgagg ggatctctca gcgttaaggg attttggtca tgcattctag gtactaaaac
    11161 aattcatcca gtaaaatata atattttatt ttctcccaat caggcttgat ccccagtaag
    11221 tcaaaaaata gctcgacata ctgttcttcc ccgatatcct ccctgatcga ccggacgcag
    11281 aaggcaatgt cataccactt gtccgccctg ccgcttctcc caagatcaat aaagccactt
    11341 actttgccat ctttcacaaa gatgttgctg tctcccaggt cgccgtggga aaagacaagt
    11401 tcctcttcgg gcttttccgt ctttaaaaaa tcatacagct cgcgcggatc tttaaatgga
    11461 gtgtcttctt cccagttttc gcaatccaca tcggccagat cgttattcag taagtaatcc
    11521 aattcggcta agcggctgtc taagctattc gtatagggac aatccgatat gtcgatggag
    11581 tgaaagagcc tgatgcactc cgcatacagc tcgataatct tttcagggct ttgttcatct
    11641 tcatactctt ccgagcaaag gacgccatcg gcctcactca tgagcagatt gctccagcca
    11701 tcatgccgtt caaagtgcag gacctttgga acaggcagct ttccttccag ccatagcatc
    11761 atgtcctttt cccgttccac atcataggtg gtccctttat accggctgtc cgtcattttt
    11821 aaatataggt tttcattttc tcccaccagc ttatatacct tagcaggaga cattccttcc
    11881 gtatctttta cgcagcggta tttttcgatc agttttttca attccggtga tattctcatt
    11941 ttagccattt attatttcct tcctcttttc tacagtattt aaagataccc caagaagcta
    12001 attataacaa gacgaactcc aattcactgt tccttgcatt ctaaaacctt aaataccaga
    12061 aaacagcttt ttcaaagttg ttttcaaagt tggcgtataa catagtatcg acggagccga
    12121 ttttgaaacc gcggtgatca caggcagcaa cgctctgtca tcgttacaat caacatgcta
    12181 ccctccgcga gatcatccgt gtttcaaacc cggcagctta gttgccgttc ttccgaatag
    12241 catcggtaac atgagcaaag tctgccgcct tacaacggct ctcccgctga cgccgtcccg
    12301 gactgatggg ctgcctgtat cgagtggtga ttttgtgccg agctgccggt cggggagctg
    12361 ttggctggct ggtggcagga tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa
    12421 taacacattg cggacgtttt taatgtactg aattaacgcc gaattaattc ctaggccacc
    12481 atgttgggcc cggcgcgcc
//
