LOCUS       sequence-502156-       12504 bp    DNA     circular SYN 09-MAR-2026
DEFINITION  Editing Photene desaturase (PDS) genes in Nicotiana benthamiana.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12504)
  AUTHORS   Ugrappa N, Rodriguez JE, Nguyen T, Weissman RF, Thornton BW, Terrace
            CI, Savage DF, Dinesh-Kumar SP
  TITLE     High-efficiency, transgene-free plant genome editing by viral 
            delivery of an engineered TnpB
  JOURNAL   Nat. Plants (2026). 20 February 2026.
REFERENCE   2  (bases 1 to 12504)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 9, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat. Plants
            (2026). 20 February 2026."
COMMENT     Sequence Label: 4932 TRV2-TnpB2xSV40nls-HHreRNA4PDS
FEATURES             Location/Qualifiers
     source          1..12504
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        427..772
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     715..735
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             3588..3608
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3615..3635
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     ncRNA           3828..3895
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
     CDS             4434..4451
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      4486..4738
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    4760..4784
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6085..6714
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7143..8216
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      8282..8476
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(8712..8734)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    8820..8960
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     8875..8894
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8975..8992)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9146..9734)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9226..9245)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(11159..11953)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    12378..12402
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 aagcttgcat gcctgcaggt caacatggtg gagcacgaca ctctcgtcta ctccaagaat
       61 atcaaagata cagtctcaga agaccagagg gctattgaga cttttcaaca aagggtaata
      121 tcgggaaacc tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta
      181 gaaaaggaag atggcttcta caaatgccat cattgcgata aaggaaaggc tatcgttcaa
      241 gatgcctcta ccgacagtgg tcccaaagat ggacccccac ccacgaggaa catcgtggaa
      301 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgatgg tcaacatggt
      361 ggagcacgac actctcgtct actccaagaa tatcaaagat acagtctcag aagaccagag
      421 ggctattgag acttttcaac aaagggtaat atcgggaaac ctcctcggat tccattgccc
      481 agctatctgt cacttcatcg aaaggacagt agaaaaggaa gatggcttct acaaatgcca
      541 tcattgcgat aaaggaaagg ctatcgttca agatgcctct accgacagtg gtcccaaaga
      601 tggaccccca cccacgagga acatcgtgga aaaagaagac gttccaacca cgtcttcaaa
      661 gcaagtggat tgatgtgata tctccactga cgtaagggat gacgcacaat cccactatcc
      721 ttcgcaagac ccttcctcta tataaggaag ttcatttcat ttggagagga taaaacattg
      781 cacctatggt gttgccctgg ctggggtatg tcagtgatcg cagtagaatg tactaattga
      841 caagttggag aatacggtag aacgtcctta tccaacacag cctttatccc tctccctgac
      901 gaggtttttg tcagtgtaat atttcttttt gaactatcca gcttagtacc gtacgggaaa
      961 gtgactggtg tgcttatctt tgaaatgtta ctttgggttt cggttcttta ggttagtaag
     1021 aaagcacttg tcttctcata caaaggaaaa cctgagacgt atcgcttacg aaagtagcaa
     1081 tgaaagaaag gtggtggttt taatcgctac cgcaaaaacg atggggtcgt tttaattaac
     1141 ttctcctacg caagcgtcta aacggacgtt ggggttttgc tagtttcttt agagaaaact
     1201 agctaagtct ttaatgttat cattagagat ggcataaata taatacttgt gtctgctgat
     1261 aagatcattt taatttggac gattagactt gttgaactac aggttactga atcacttgcg
     1321 ctaatcaaca tgggagatat gtacgatgaa tcatttgaca agtcgggcgg tcctgctgac
     1381 ttgatggacg attcttgggt ggaatcagtt tcgtggaaag atctgttgaa gaagttacac
     1441 agcataaaat ttgcactaca gtctggtaga gatgagatca ctgggttact agcggcactg
     1501 aatagacagt gtccttattc accatatgag cagtttccag ataagaaggt gtatttcctt
     1561 ttagactcac gggctaacag tgctcttggt gtgattcaga acgcttcagc gttcaagaga
     1621 cgagctgatg agaagaatgc agtggcgggt gttacaaata ttcctgcgaa tccaaacaca
     1681 acggttacga cgaaccaagg gagtactact actaccaagg cgaacactgg ctcgactttg
     1741 gaagaagact tgtacactta ttacaaattc gatgatgcct ctacagcttt ccacaaatct
     1801 ctaacttcgt tagagaacat ggagttgaag agttattacc gaaggaactt tgagaaagta
     1861 ttcgggatta agtttggtgg agcagctgct agttcatctg caccgcctcc agcgagtgga
     1921 ggtccgatac gtcctaatcc ctagggattt aaggacgtga actctgttga gatctctgtg
     1981 aaattcagag ggtgggtgat accatattca ctgatgccat tagcgacatc taaatagggc
     2041 taattgtgac taatttgagg gaatttcctt taccattgac gtcagtgtcg ttggtagcat
     2101 ttgagtttcg gcacacaagg ttaaaaacgc tgtagtaata catgcgcaag aacaggctga
     2161 gcatcttgtt ctggggtttc acactatctt tagagaaagt gttaagttaa ttaagttatc
     2221 ttaattaaga gcataattat actgatttgt ctctcgttga tagagtctat cattctgtta
     2281 ttaaaaattt gacaactcgg tttgctgacc tactggtcac tgtatcactt acccgagtta
     2341 acgagtctag aatgatacgc aataaggcat ttgtggttcg gctctacccg aatgcggcac
     2401 aaactgagct gataaaccga acactggggt ctgcacgatt tgtatacaat catttccttg
     2461 ctcgaagaat cgctgcgtac aaggaaagcg gtaagggtct tacgtacggg caaacttcta
     2521 gtgagcttac tctgctgaag caagcagagg aaacctcctg gctgtcagaa gtggataaat
     2581 tcgcgttgca gaatagcttg aaaaaccttg aaacggccta taaaaacttc tttagaacag
     2641 ttaagcagtc aggaaaaaag gtaggtttcc ctcggttcag gaagaagcgc acgggggagt
     2701 cataccgcac gcagtttacg aacaacaaca ttcagatagg agaggggagg cttaaactgc
     2761 cgaaactggg gtgggtcaaa actaagggtc agcaggatat ccaaggcaaa atattgaatg
     2821 ttactgtacg ccgcatccat gaaggccatt atgaagcttc agtgctgtgc gaggtcgaaa
     2881 tcccttacct cccagctgct ccgaaattcg cggcaggagt agacgtgggg attaaagact
     2941 ttgcgatcgt caccgatggt gtgcgattta agcatgagca aaatcctaaa tactatcgga
     3001 gtacgcttaa gcgactccgg aaggcccaac agactctgtc caggagaaag aaaggaagcg
     3061 ctagatatgg caaggctaag actaagctgg caaggattca caagcgcatc gtgaataaga
     3121 gacaagattt tctgcacaaa ctgaccactt cccttgtgcg cgagtatgaa ataatcggga
     3181 ctgaacacct gaagcccgac aatatgcgca agaatagaag gctggcgctc agcatctcag
     3241 atgctggatg gggtgaattc ataaggcagt tggagtacaa agctgcttgg tatgggagac
     3301 tcgtaagtaa agtttcccca tattttcctt ctagtcaact ctgtcacgac tgcggattca
     3361 aaaaccccga agtgaaaaat ctcgcggtaa ggacttggac gtgcccaaac tgtggcgaga
     3421 cacacgacag agatgaaaat gcggcgctga atatcaggag agaggcgttg gtggctgcgg
     3481 ggatttcaga tacccttaac gcgcacggcg gttacgtgcg acccgcttcc gccggtaacg
     3541 gccttaggag tgaaaaccac gctactctcg tagtgagcag ggccgaccct aaaaagaaaa
     3601 gaaaggtgga agaccctaag aagaagagga aagtgtgagg taccttcttg aatcctgatg
     3661 agtccgtgag gacgaaacga gctagctcgt cggtggctgc gggaatctca gacaccttaa
     3721 acgctcatgg aggctatgtc agacctgctt cggcgggcaa tggtctgcga agtgagaatc
     3781 acgcgacttt agtcgtgtga ggttcaagct acaatgaagg aactagcggc cggcatggtc
     3841 ccagcctcct cgctggcgcc ggctgggcaa catgcttcgg catggcgaat gggacgagct
     3901 cccgtagctc agttggttag agcgttggtc ttatgagccg aaggtcgcgg gttcgagccc
     3961 cgccggaagc acccgggcat gtcccgaaga cattaaacta cggttcttta agtagatccg
     4021 tgtctgaagt tttaggttca atttaaacct acgagattga cattctcgac tgatcttgat
     4081 tgatcggtaa gtcttttgta atttaatttt ctttttgatt ttattttaaa ttgttatctg
     4141 tttctgtgta tagactgttt gagatcggcg tttggccgac tcattgtctt accatagggg
     4201 aacggacttt gtttgtgttg ttattttatt tgtattttat taaaattctc aacgatctga
     4261 aaaagcctcg cggctaagag attgttgggg ggtgagtaag tacttttaaa gtgatgatgg
     4321 ttacaaaggc aaaaggggta aaacccctcg cctacgtaag cgttattacg cccgtctgta
     4381 cttatatcag tacactgacg agtccctaaa ggacgaaacg ggagaacgct agccaccacc
     4441 accaccacca cgtgtgaatt acaggtgacc agctcgaatt tccccgatcg ttcaaacatt
     4501 tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat tatcatataa
     4561 tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg
     4621 agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat agaaaacaaa
     4681 atatagcgcg caaactagga taaattatcg cgcgcggtgt catctatgtt actagatcgg
     4741 gaattaaact atcagtgttt gacaggatat attggcgggt aaacctaaga gaaaagagcg
     4801 tttattagaa taatcggata tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg
     4861 tatgtgcatg ccaaccacag ggttcccctc gggatcaaag tactttgatc caacccctcc
     4921 gctgctatag tgcagtcggc ttctgacgtt cagtgcagcc gtcttctgaa aacgacatgt
     4981 cgcacaagtc ctaagttacg cgacaggctg ccgccctgcc cttttcctgg cgttttcttg
     5041 tcgcgtgttt tagtcgcata aagtagaata cttgcgacta gaaccggaga cattacgcca
     5101 tgaacaagag cgccgccgct ggcctgctgg gctatgcccg cgtcagcacc gacgaccagg
     5161 acttgaccaa ccaacgggcc gaactgcacg cggccggctg caccaagctg ttttccgaga
     5221 agatcaccgg caccaggcgc gaccgcccgg agctggccag gatgcttgac cacctacgcc
     5281 ctggcgacgt tgtgacagtg accaggctag accgcctggc ccgcagcacc cgcgacctac
     5341 tggacattgc cgagcgcatc caggaggccg gcgcgggcct gcgtagcctg gcagagccgt
     5401 gggccgacac caccacgccg gccggccgca tggtgttgac cgtgttcgcc ggcattgccg
     5461 agttcgagcg ttccctaatc atcgaccgca cccggagcgg gcgcgaggcc gccaaggccc
     5521 gaggcgtgaa gtttggcccc cgccctaccc tcaccccggc acagatcgcg cacgcccgcg
     5581 agctgatcga ccaggaaggc cgcaccgtga aagaggcggc tgcactgctt ggcgtgcatc
     5641 gctcgaccct gtaccgcgca cttgagcgca gcgaggaagt gacgcccacc gaggccaggc
     5701 ggcgcggtgc cttccgtgag gacgcattga ccgaggccga cgccctggcg gccgccgaga
     5761 atgaacgcca agaggaacaa gcatgaaacc gcaccaggac ggccaggacg aaccgttttt
     5821 cattaccgaa gagatcgagg cggagatgat cgcggccggg tacgtgttcg agccgcccgc
     5881 gcacgtctca accgtgcggc tgcatgaaat cctggccggt ttgtctgatg ccaagctggc
     5941 ggcctggccg gccagcttgg ccgctgaaga aaccgagcgc cgccgtctaa aaaggtgatg
     6001 tgtatttgag taaaacagct tgcgtcatgc ggtcgctgcg tatatgatgc gatgagtaaa
     6061 taaacaaata cgcaagggga acgcatgaag gttatcgctg tacttaacca gaaaggcggg
     6121 tcaggcaaga cgaccatcgc aacccatcta gcccgcgccc tgcaactcgc cggggccgat
     6181 gttctgttag tcgattccga tccccagggc agtgcccgcg attgggcggc cgtgcgggaa
     6241 gatcaaccgc taaccgttgt cggcatcgac cgcccgacga ttgaccgcga cgtgaaggcc
     6301 atcggccggc gcgacttcgt agtgatcgac ggagcgcccc aggcggcgga cttggctgtg
     6361 tccgcgatca aggcagccga cttcgtgctg attccggtgc agccaagccc ttacgacata
     6421 tgggccaccg ccgacctggt ggagctggtt aagcagcgca ttgaggtcac ggatggaagg
     6481 ctacaagcgg cctttgtcgt gtcgcgggcg atcaaaggca cgcgcatcgg cggtgaggtt
     6541 gccgaggcgc tggccgggta cgagctgccc attcttgagt cccgtatcac gcagcgcgtg
     6601 agctacccag gcactgccgc cgccggcaca accgttcttg aatcagaacc cgagggcgac
     6661 gctgcccgcg aggtccaggc gctggccgct gaaattaaat caaaactcat ttgagttaat
     6721 gaggtaaaga gaaaatgagc aaaagcacaa acacgctaag tgccggccgt ccgagcgcac
     6781 gcagcagcaa ggctgcaacg ttggccagcc tggcagacac gccagccatg aagcgggtca
     6841 actttcagtt gccggcggag gatcacacca agctgaagat gtacgcggta cgccaaggca
     6901 agaccattac cgagctgcta tctgaataca tcgcgcagct accagagtaa atgagcaaat
     6961 gaataaatga gtagatgaat tttagcggct aaaggaggcg gcatggaaaa tcaagaacaa
     7021 ccaggcaccg acgccgtgga atgccccatg tgtggaggaa cgggcggttg gccaggcgta
     7081 agcggctggg ttgcctgccg gccctgcaat ggcactggaa cccccaagcc cgaggaatcg
     7141 gcgtgagcgg tcgcaaacca tccggcccgg tacaaatcgg cgcggcgctg ggtgatgacc
     7201 tggtggagaa gttgaaggcc gcgcaggccg cccagcggca acgcatcgag gcagaagcac
     7261 gccccggtga atcgtggcaa gcggccgctg atcgaatccg caaagaatcc cggcaaccgc
     7321 cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg cgacgagcaa ccagattttt
     7381 tcgttccgat gctctatgac gtgggcaccc gcgatagtcg cagcatcatg gacgtggccg
     7441 ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt gatccgctac gagcttccag
     7501 acgggcacgt agaggtttcc gcagggccgg ccggcatggc cagtgtgtgg gattacgacc
     7561 tggtactgat ggcggtttcc catctaaccg aatccatgaa ccgataccgg gaagggaagg
     7621 gagacaagcc cggccgcgtg ttccgtccac acgttgcgga cgtactcaag ttctgccggc
     7681 gagccgatgg cggaaagcag aaagacgacc tggtagaaac ctgcattcgg ttaaacacca
     7741 cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg ccgcctggtg acggtatccg
     7801 agggtgaagc cttgattagc cgctacaaga tcgtaaagag cgaaaccggg cggccggagt
     7861 acatcgagat cgagctagct gattggatgt accgcgagat cacagaaggc aagaacccgg
     7921 acgtgctgac ggttcacccc gattactttt tgatcgatcc cggcatcggc cgttttctct
     7981 accgcctggc acgccgcgcc gcaggcaagg cagaagccag atggttgttc aagacgatct
     8041 acgaacgcag tggcagcgcc ggagagttca agaagttctg tttcaccgtg cgcaagctga
     8101 tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga ggcggggcag gctggcccga
     8161 tcctagtcat gcgctaccgc aacctgatcg agggcgaagc atccgccggt tcctaatgta
     8221 cggagcagat gctagggcaa attgccctag caggggaaaa aggtcgaaaa ggtctctttc
     8281 ctgtggatag cacgtacatt gggaacccaa agccgtacat tgggaaccgg aacccgtaca
     8341 ttgggaaccc aaagccgtac attgggaacc ggtcacacat gtaagtgact gatataaaag
     8401 agaaaaaagg cgatttttcc gcctaaaact ctttaaaact tattaaaact cttaaaaccc
     8461 gcctggcctg tgcataactg tctggccagc gcacagccga agagctgcaa aaagcgccta
     8521 cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg tcggcctatc gcggccgctg
     8581 gccgctcaaa aatggctggc ctacggccag gcaatctacc agggcgcgga caagccgcgc
     8641 cgtcgccact cgaccgccgg cgcccacatc aaggcaccct gcctcgcgcg tttcggtgat
     8701 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
     8761 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
     8821 gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac tatgcggcat
     8881 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa
     8941 ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     9001 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     9061 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     9121 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     9181 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     9241 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     9301 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     9361 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     9421 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     9481 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     9541 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     9601 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     9661 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     9721 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     9781 aaaactcacg ttaagggctg atgaatcccc taatgatttt tatcaaaatc attaagttaa
     9841 ggtagataca catcttgtca tatgatcaaa tggtttcgcc aaaaatcaat aatcagacaa
     9901 caaaatgtgc gaactcgata ttttacacga ctctctttac caattctgcc ccgaattaca
     9961 cttaaaacga ctcaacagct taacgttggc ttgccacgcc ttacttgact gtaaaactct
    10021 cactcttacc gaacttggcc gtaacctgcc aaccaaagcg agaacaaaac ataacatcaa
    10081 acgaatcgac cgattgttag gtaatcgtca cctccacaaa gagcgactcg ctgtataccg
    10141 ttggcatgct agctttatct gttcgggcaa tacgatgccc attgtacttg ttgactggtc
    10201 tgatatccgt gagcaaaaac ggcttatggt attgcgagct tcagtcgcac tacacggtcg
    10261 ttctgttact ctttatgaga aagcgttccc gctttcagag caatgttcaa agaaagctca
    10321 tgaccaattt ctagccgacc ttgcgagcat tctaccgagt aacaccacac cgctcattgt
    10381 cagtgatgct ggctttaaag tgccatggta taaatccgtt gagaagctgg gttggtactg
    10441 gttaagtcga gtaagaggaa aagtacaata tgcagaccta ggagcggaaa actggaaacc
    10501 tatcagcaac ttacatgata tgtcatctag tcactcaaag actttaggct ataagaggct
    10561 gactaaaagc aatccaatct catgccaaat tctattgtat aaatctcgct ctaaaggccg
    10621 aaaaaatcag cgctcgacac ggactcattg tcaccacccg tcacctaaaa tctactcagc
    10681 gtcggcaaag gagccatgga ttctagcaac taacttacct gttgaaattc gaacacccaa
    10741 acaacttgtt aatatctatt cgaagcgaat gcagattgaa gaaaccttcc gagacttgaa
    10801 aagtcctgcc tacggactag gcctacgcca tagccgaacg agcagctcag agcgttttga
    10861 tatcatgctg ctaatcgccc tgatgcttca actaacatgt tggcttgcgg gcgttcatgc
    10921 tcagaaacaa ggttgggaca agcacttcca ggctaacaca gtcagaaatc gaaacgtact
    10981 ctcaacagtt cgcttaggca tggaagtttt gcggcattct ggctacacaa taacaaggga
    11041 agactcactc gtggctgcaa ccctgcttac tcaaaatcta ttcacacatg gttacgtttt
    11101 ggggaaatta tgaggggatc tctcagcgtt aagggatttt ggtcatgcat tctaggtact
    11161 aaaacaattc atccagtaaa atataatatt ttattttctc ccaatcaggc ttgatcccca
    11221 gtaagtcaaa aaatagctcg acatactgtt cttccccgat atcctccctg atcgaccgga
    11281 cgcagaaggc aatgtcatac cacttgtccg ccctgccgct tctcccaaga tcaataaagc
    11341 cacttacttt gccatctttc acaaagatgt tgctgtctcc caggtcgccg tgggaaaaga
    11401 caagttcctc ttcgggcttt tccgtcttta aaaaatcata cagctcgcgc ggatctttaa
    11461 atggagtgtc ttcttcccag ttttcgcaat ccacatcggc cagatcgtta ttcagtaagt
    11521 aatccaattc ggctaagcgg ctgtctaagc tattcgtata gggacaatcc gatatgtcga
    11581 tggagtgaaa gagcctgatg cactccgcat acagctcgat aatcttttca gggctttgtt
    11641 catcttcata ctcttccgag caaaggacgc catcggcctc actcatgagc agattgctcc
    11701 agccatcatg ccgttcaaag tgcaggacct ttggaacagg cagctttcct tccagccata
    11761 gcatcatgtc cttttcccgt tccacatcat aggtggtccc tttataccgg ctgtccgtca
    11821 tttttaaata taggttttca ttttctccca ccagcttata taccttagca ggagacattc
    11881 cttccgtatc ttttacgcag cggtattttt cgatcagttt tttcaattcc ggtgatattc
    11941 tcattttagc catttattat ttccttcctc ttttctacag tatttaaaga taccccaaga
    12001 agctaattat aacaagacga actccaattc actgttcctt gcattctaaa accttaaata
    12061 ccagaaaaca gctttttcaa agttgttttc aaagttggcg tataacatag tatcgacgga
    12121 gccgattttg aaaccgcggt gatcacaggc agcaacgctc tgtcatcgtt acaatcaaca
    12181 tgctaccctc cgcgagatca tccgtgtttc aaacccggca gcttagttgc cgttcttccg
    12241 aatagcatcg gtaacatgag caaagtctgc cgccttacaa cggctctccc gctgacgccg
    12301 tcccggactg atgggctgcc tgtatcgagt ggtgattttg tgccgagctg ccggtcgggg
    12361 agctgttggc tggctggtgg caggatatat tgtggtgtaa acaaattgac gcttagacaa
    12421 cttaataaca cattgcggac gtttttaatg tactgaatta acgccgaatt aattcctagg
    12481 ccaccatgtt gggcccggcg cgcc
//
