LOCUS       sequence-502158-       12546 bp    DNA     circular SYN 09-MAR-2026
DEFINITION  Editing Photene desaturase (PDS) genes in Nicotiana benthamiana.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12546)
  AUTHORS   Ugrappa N, Rodriguez JE, Nguyen T, Weissman RF, Thornton BW, Terrace
            CI, Savage DF, Dinesh-Kumar SP
  TITLE     High-efficiency, transgene-free plant genome editing by viral 
            delivery of an engineered TnpB
  JOURNAL   Nat. Plants (2026). 20 February 2026.
REFERENCE   2  (bases 1 to 12546)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 9, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat. Plants
            (2026). 20 February 2026."
COMMENT     Sequence Label: 4928 TRV2-TnpB-HHreRNA4PDS
FEATURES             Location/Qualifiers
     source          1..12546
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        427..772
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     715..735
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     ncRNA           3870..3937
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
     CDS             4476..4493
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      4528..4780
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    4802..4826
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6127..6756
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7185..8258
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      8324..8518
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(8754..8776)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    8862..9002
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     8917..8936
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(9017..9034)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9188..9776)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9268..9287)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(11201..11995)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    12420..12444
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 aagcttgcat gcctgcaggt caacatggtg gagcacgaca ctctcgtcta ctccaagaat
       61 atcaaagata cagtctcaga agaccagagg gctattgaga cttttcaaca aagggtaata
      121 tcgggaaacc tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta
      181 gaaaaggaag atggcttcta caaatgccat cattgcgata aaggaaaggc tatcgttcaa
      241 gatgcctcta ccgacagtgg tcccaaagat ggacccccac ccacgaggaa catcgtggaa
      301 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgatgg tcaacatggt
      361 ggagcacgac actctcgtct actccaagaa tatcaaagat acagtctcag aagaccagag
      421 ggctattgag acttttcaac aaagggtaat atcgggaaac ctcctcggat tccattgccc
      481 agctatctgt cacttcatcg aaaggacagt agaaaaggaa gatggcttct acaaatgcca
      541 tcattgcgat aaaggaaagg ctatcgttca agatgcctct accgacagtg gtcccaaaga
      601 tggaccccca cccacgagga acatcgtgga aaaagaagac gttccaacca cgtcttcaaa
      661 gcaagtggat tgatgtgata tctccactga cgtaagggat gacgcacaat cccactatcc
      721 ttcgcaagac ccttcctcta tataaggaag ttcatttcat ttggagagga taaaacattg
      781 cacctatggt gttgccctgg ctggggtatg tcagtgatcg cagtagaatg tactaattga
      841 caagttggag aatacggtag aacgtcctta tccaacacag cctttatccc tctccctgac
      901 gaggtttttg tcagtgtaat atttcttttt gaactatcca gcttagtacc gtacgggaaa
      961 gtgactggtg tgcttatctt tgaaatgtta ctttgggttt cggttcttta ggttagtaag
     1021 aaagcacttg tcttctcata caaaggaaaa cctgagacgt atcgcttacg aaagtagcaa
     1081 tgaaagaaag gtggtggttt taatcgctac cgcaaaaacg atggggtcgt tttaattaac
     1141 ttctcctacg caagcgtcta aacggacgtt ggggttttgc tagtttcttt agagaaaact
     1201 agctaagtct ttaatgttat cattagagat ggcataaata taatacttgt gtctgctgat
     1261 aagatcattt taatttggac gattagactt gttgaactac aggttactga atcacttgcg
     1321 ctaatcaaca tgggagatat gtacgatgaa tcatttgaca agtcgggcgg tcctgctgac
     1381 ttgatggacg attcttgggt ggaatcagtt tcgtggaaag atctgttgaa gaagttacac
     1441 agcataaaat ttgcactaca gtctggtaga gatgagatca ctgggttact agcggcactg
     1501 aatagacagt gtccttattc accatatgag cagtttccag ataagaaggt gtatttcctt
     1561 ttagactcac gggctaacag tgctcttggt gtgattcaga acgcttcagc gttcaagaga
     1621 cgagctgatg agaagaatgc agtggcgggt gttacaaata ttcctgcgaa tccaaacaca
     1681 acggttacga cgaaccaagg gagtactact actaccaagg cgaacactgg ctcgactttg
     1741 gaagaagact tgtacactta ttacaaattc gatgatgcct ctacagcttt ccacaaatct
     1801 ctaacttcgt tagagaacat ggagttgaag agttattacc gaaggaactt tgagaaagta
     1861 ttcgggatta agtttggtgg agcagctgct agttcatctg caccgcctcc agcgagtgga
     1921 ggtccgatac gtcctaatcc ctagggattt aaggacgtga actctgttga gatctctgtg
     1981 aaattcagag ggtgggtgat accatattca ctgatgccat tagcgacatc taaatagggc
     2041 taattgtgac taatttgagg gaatttcctt taccattgac gtcagtgtcg ttggtagcat
     2101 ttgagtttcg gcacacaagg ttaaaaacgc tgtagtaata catgcgcaag aacaggctga
     2161 gcatcttgtt ctggggtttc acactatctt tagagaaagt gttaagttaa ttaagttatc
     2221 ttaattaaga gcataattat actgatttgt ctctcgttga tagagtctat cattctgtta
     2281 ttaaaaattt gacaactcgg tttgctgacc tactggtcac tgtatcactt acccgagtta
     2341 acgagtctag aatgatacgc aataaggcat ttgtggttcg gctctacccg aatgcggcac
     2401 aaactgagct gataaaccga acactggggt ctgcacgatt tgtatacaat catttccttg
     2461 ctcgaagaat cgctgcgtac aaggaaagcg gtaagggtct tacgtacggg caaacttcta
     2521 gtgagcttac tctgctgaag caagcagagg aaacctcctg gctgtcagaa gtggataaat
     2581 tcgcgttgca gaatagcttg aaaaaccttg aaacggccta taaaaacttc tttagaacag
     2641 ttaagcagtc aggaaaaaag gtaggtttcc ctcggttcag gaagaagcgc acgggggagt
     2701 cataccgcac gcagtttacg aacaacaaca ttcagatagg agaggggagg cttaaactgc
     2761 cgaaactggg gtgggtcaaa actaagggtc agcaggatat ccaaggcaaa atattgaatg
     2821 ttactgtacg ccgcatccat gaaggccatt atgaagcttc agtgctgtgc gaggtcgaaa
     2881 tcccttacct cccagctgct ccgaaattcg cggcaggagt agacgtgggg attaaagact
     2941 ttgcgatcgt caccgatggt gtgcgattta agcatgagca aaatcctaaa tactatcgga
     3001 gtacgcttaa gcgactccgg aaggcccaac agactctgtc caggagaaag aaaggaagcg
     3061 ctagatatgg caaggctaag actaagctgg caaggattca caagcgcatc gtgaataaga
     3121 gacaagattt tctgcacaaa ctgaccactt cccttgtgcg cgagtatgaa ataatcggga
     3181 ctgaacacct gaagcccgac aatatgcgca agaatagaag gctggcgctc agcatctcag
     3241 atgctggatg gggtgaattc ataaggcagt tggagtacaa agctgcttgg tatgggagac
     3301 tcgtaagtaa agtttcccca tattttcctt ctagtcaact ctgtcacgac tgcggattca
     3361 aaaaccccga agtgaaaaat ctcgcggtaa ggacttggac gtgcccaaac tgtggcgaga
     3421 cacacgacag agatgaaaat gcggcgctga atatcaggag agaggcgttg gtggctgcgg
     3481 ggatttcaga tacccttaac gcgcacggcg gttacgtgcg acccgcttcc gccggtaacg
     3541 gccttaggag tgaaaaccac gctactctcg tagtgagcag ggccgacgaa ggttcaggga
     3601 atagaagaca taagcgtatg atcaagaaca gagaatctgc agctcgttcc cgcgctagga
     3661 aacaggctta tacaaactag ggtaccttct tgaatcctga tgagtccgtg aggacgaaac
     3721 gagctagctc gtcggtggct gcgggaatct cagacacctt aaacgctcat ggaggctatg
     3781 tcagacctgc ttcggcgggc aatggtctgc gaagtgagaa tcacgcgact ttagtcgtgt
     3841 gaggttcaag ctacaatgaa ggaactagcg gccggcatgg tcccagcctc ctcgctggcg
     3901 ccggctgggc aacatgcttc ggcatggcga atgggacgag ctcccgtagc tcagttggtt
     3961 agagcgttgg tcttatgagc cgaaggtcgc gggttcgagc cccgccggaa gcacccgggc
     4021 atgtcccgaa gacattaaac tacggttctt taagtagatc cgtgtctgaa gttttaggtt
     4081 caatttaaac ctacgagatt gacattctcg actgatcttg attgatcggt aagtcttttg
     4141 taatttaatt ttctttttga ttttatttta aattgttatc tgtttctgtg tatagactgt
     4201 ttgagatcgg cgtttggccg actcattgtc ttaccatagg ggaacggact ttgtttgtgt
     4261 tgttatttta tttgtatttt attaaaattc tcaacgatct gaaaaagcct cgcggctaag
     4321 agattgttgg ggggtgagta agtactttta aagtgatgat ggttacaaag gcaaaagggg
     4381 taaaacccct cgcctacgta agcgttatta cgcccgtctg tacttatatc agtacactga
     4441 cgagtcccta aaggacgaaa cgggagaacg ctagccacca ccaccaccac cacgtgtgaa
     4501 ttacaggtga ccagctcgaa tttccccgat cgttcaaaca tttggcaata aagtttctta
     4561 agattgaatc ctgttgccgg tcttgcgatg attatcatat aatttctgtt gaattacgtt
     4621 aagcatgtaa taattaacat gtaatgcatg acgttattta tgagatgggt ttttatgatt
     4681 agagtcccgc aattatacat ttaatacgcg atagaaaaca aaatatagcg cgcaaactag
     4741 gataaattat cgcgcgcggt gtcatctatg ttactagatc gggaattaaa ctatcagtgt
     4801 ttgacaggat atattggcgg gtaaacctaa gagaaaagag cgtttattag aataatcgga
     4861 tatttaaaag ggcgtgaaaa ggtttatccg ttcgtccatt tgtatgtgca tgccaaccac
     4921 agggttcccc tcgggatcaa agtactttga tccaacccct ccgctgctat agtgcagtcg
     4981 gcttctgacg ttcagtgcag ccgtcttctg aaaacgacat gtcgcacaag tcctaagtta
     5041 cgcgacaggc tgccgccctg cccttttcct ggcgttttct tgtcgcgtgt tttagtcgca
     5101 taaagtagaa tacttgcgac tagaaccgga gacattacgc catgaacaag agcgccgccg
     5161 ctggcctgct gggctatgcc cgcgtcagca ccgacgacca ggacttgacc aaccaacggg
     5221 ccgaactgca cgcggccggc tgcaccaagc tgttttccga gaagatcacc ggcaccaggc
     5281 gcgaccgccc ggagctggcc aggatgcttg accacctacg ccctggcgac gttgtgacag
     5341 tgaccaggct agaccgcctg gcccgcagca cccgcgacct actggacatt gccgagcgca
     5401 tccaggaggc cggcgcgggc ctgcgtagcc tggcagagcc gtgggccgac accaccacgc
     5461 cggccggccg catggtgttg accgtgttcg ccggcattgc cgagttcgag cgttccctaa
     5521 tcatcgaccg cacccggagc gggcgcgagg ccgccaaggc ccgaggcgtg aagtttggcc
     5581 cccgccctac cctcaccccg gcacagatcg cgcacgcccg cgagctgatc gaccaggaag
     5641 gccgcaccgt gaaagaggcg gctgcactgc ttggcgtgca tcgctcgacc ctgtaccgcg
     5701 cacttgagcg cagcgaggaa gtgacgccca ccgaggccag gcggcgcggt gccttccgtg
     5761 aggacgcatt gaccgaggcc gacgccctgg cggccgccga gaatgaacgc caagaggaac
     5821 aagcatgaaa ccgcaccagg acggccagga cgaaccgttt ttcattaccg aagagatcga
     5881 ggcggagatg atcgcggccg ggtacgtgtt cgagccgccc gcgcacgtct caaccgtgcg
     5941 gctgcatgaa atcctggccg gtttgtctga tgccaagctg gcggcctggc cggccagctt
     6001 ggccgctgaa gaaaccgagc gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag
     6061 cttgcgtcat gcggtcgctg cgtatatgat gcgatgagta aataaacaaa tacgcaaggg
     6121 gaacgcatga aggttatcgc tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc
     6181 gcaacccatc tagcccgcgc cctgcaactc gccggggccg atgttctgtt agtcgattcc
     6241 gatccccagg gcagtgcccg cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt
     6301 gtcggcatcg accgcccgac gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc
     6361 gtagtgatcg acggagcgcc ccaggcggcg gacttggctg tgtccgcgat caaggcagcc
     6421 gacttcgtgc tgattccggt gcagccaagc ccttacgaca tatgggccac cgccgacctg
     6481 gtggagctgg ttaagcagcg cattgaggtc acggatggaa ggctacaagc ggcctttgtc
     6541 gtgtcgcggg cgatcaaagg cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg
     6601 tacgagctgc ccattcttga gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc
     6661 gccgccggca caaccgttct tgaatcagaa cccgagggcg acgctgcccg cgaggtccag
     6721 gcgctggccg ctgaaattaa atcaaaactc atttgagtta atgaggtaaa gagaaaatga
     6781 gcaaaagcac aaacacgcta agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa
     6841 cgttggccag cctggcagac acgccagcca tgaagcgggt caactttcag ttgccggcgg
     6901 aggatcacac caagctgaag atgtacgcgg tacgccaagg caagaccatt accgagctgc
     6961 tatctgaata catcgcgcag ctaccagagt aaatgagcaa atgaataaat gagtagatga
     7021 attttagcgg ctaaaggagg cggcatggaa aatcaagaac aaccaggcac cgacgccgtg
     7081 gaatgcccca tgtgtggagg aacgggcggt tggccaggcg taagcggctg ggttgcctgc
     7141 cggccctgca atggcactgg aacccccaag cccgaggaat cggcgtgagc ggtcgcaaac
     7201 catccggccc ggtacaaatc ggcgcggcgc tgggtgatga cctggtggag aagttgaagg
     7261 ccgcgcaggc cgcccagcgg caacgcatcg aggcagaagc acgccccggt gaatcgtggc
     7321 aagcggccgc tgatcgaatc cgcaaagaat cccggcaacc gccggcagcc ggtgcgccgt
     7381 cgattaggaa gccgcccaag ggcgacgagc aaccagattt tttcgttccg atgctctatg
     7441 acgtgggcac ccgcgatagt cgcagcatca tggacgtggc cgttttccgt ctgtcgaagc
     7501 gtgaccgacg agctggcgag gtgatccgct acgagcttcc agacgggcac gtagaggttt
     7561 ccgcagggcc ggccggcatg gccagtgtgt gggattacga cctggtactg atggcggttt
     7621 cccatctaac cgaatccatg aaccgatacc gggaagggaa gggagacaag cccggccgcg
     7681 tgttccgtcc acacgttgcg gacgtactca agttctgccg gcgagccgat ggcggaaagc
     7741 agaaagacga cctggtagaa acctgcattc ggttaaacac cacgcacgtt gccatgcagc
     7801 gtacgaagaa ggccaagaac ggccgcctgg tgacggtatc cgagggtgaa gccttgatta
     7861 gccgctacaa gatcgtaaag agcgaaaccg ggcggccgga gtacatcgag atcgagctag
     7921 ctgattggat gtaccgcgag atcacagaag gcaagaaccc ggacgtgctg acggttcacc
     7981 ccgattactt tttgatcgat cccggcatcg gccgttttct ctaccgcctg gcacgccgcg
     8041 ccgcaggcaa ggcagaagcc agatggttgt tcaagacgat ctacgaacgc agtggcagcg
     8101 ccggagagtt caagaagttc tgtttcaccg tgcgcaagct gatcgggtca aatgacctgc
     8161 cggagtacga tttgaaggag gaggcggggc aggctggccc gatcctagtc atgcgctacc
     8221 gcaacctgat cgagggcgaa gcatccgccg gttcctaatg tacggagcag atgctagggc
     8281 aaattgccct agcaggggaa aaaggtcgaa aaggtctctt tcctgtggat agcacgtaca
     8341 ttgggaaccc aaagccgtac attgggaacc ggaacccgta cattgggaac ccaaagccgt
     8401 acattgggaa ccggtcacac atgtaagtga ctgatataaa agagaaaaaa ggcgattttt
     8461 ccgcctaaaa ctctttaaaa cttattaaaa ctcttaaaac ccgcctggcc tgtgcataac
     8521 tgtctggcca gcgcacagcc gaagagctgc aaaaagcgcc tacccttcgg tcgctgcgct
     8581 ccctacgccc cgccgcttcg cgtcggccta tcgcggccgc tggccgctca aaaatggctg
     8641 gcctacggcc aggcaatcta ccagggcgcg gacaagccgc gccgtcgcca ctcgaccgcc
     8701 ggcgcccaca tcaaggcacc ctgcctcgcg cgtttcggtg atgacggtga aaacctctga
     8761 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
     8821 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca
     8881 cgtagcgata gcggagtgta tactggctta actatgcggc atcagagcag attgtactga
     8941 gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
     9001 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     9061 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     9121 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     9181 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     9241 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     9301 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     9361 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     9421 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     9481 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     9541 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     9601 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     9661 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     9721 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     9781 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaagggc
     9841 tgatgaatcc cctaatgatt tttatcaaaa tcattaagtt aaggtagata cacatcttgt
     9901 catatgatca aatggtttcg ccaaaaatca ataatcagac aacaaaatgt gcgaactcga
     9961 tattttacac gactctcttt accaattctg ccccgaatta cacttaaaac gactcaacag
    10021 cttaacgttg gcttgccacg ccttacttga ctgtaaaact ctcactctta ccgaacttgg
    10081 ccgtaacctg ccaaccaaag cgagaacaaa acataacatc aaacgaatcg accgattgtt
    10141 aggtaatcgt cacctccaca aagagcgact cgctgtatac cgttggcatg ctagctttat
    10201 ctgttcgggc aatacgatgc ccattgtact tgttgactgg tctgatatcc gtgagcaaaa
    10261 acggcttatg gtattgcgag cttcagtcgc actacacggt cgttctgtta ctctttatga
    10321 gaaagcgttc ccgctttcag agcaatgttc aaagaaagct catgaccaat ttctagccga
    10381 ccttgcgagc attctaccga gtaacaccac accgctcatt gtcagtgatg ctggctttaa
    10441 agtgccatgg tataaatccg ttgagaagct gggttggtac tggttaagtc gagtaagagg
    10501 aaaagtacaa tatgcagacc taggagcgga aaactggaaa cctatcagca acttacatga
    10561 tatgtcatct agtcactcaa agactttagg ctataagagg ctgactaaaa gcaatccaat
    10621 ctcatgccaa attctattgt ataaatctcg ctctaaaggc cgaaaaaatc agcgctcgac
    10681 acggactcat tgtcaccacc cgtcacctaa aatctactca gcgtcggcaa aggagccatg
    10741 gattctagca actaacttac ctgttgaaat tcgaacaccc aaacaacttg ttaatatcta
    10801 ttcgaagcga atgcagattg aagaaacctt ccgagacttg aaaagtcctg cctacggact
    10861 aggcctacgc catagccgaa cgagcagctc agagcgtttt gatatcatgc tgctaatcgc
    10921 cctgatgctt caactaacat gttggcttgc gggcgttcat gctcagaaac aaggttggga
    10981 caagcacttc caggctaaca cagtcagaaa tcgaaacgta ctctcaacag ttcgcttagg
    11041 catggaagtt ttgcggcatt ctggctacac aataacaagg gaagactcac tcgtggctgc
    11101 aaccctgctt actcaaaatc tattcacaca tggttacgtt ttggggaaat tatgagggga
    11161 tctctcagcg ttaagggatt ttggtcatgc attctaggta ctaaaacaat tcatccagta
    11221 aaatataata ttttattttc tcccaatcag gcttgatccc cagtaagtca aaaaatagct
    11281 cgacatactg ttcttccccg atatcctccc tgatcgaccg gacgcagaag gcaatgtcat
    11341 accacttgtc cgccctgccg cttctcccaa gatcaataaa gccacttact ttgccatctt
    11401 tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc tcttcgggct
    11461 tttccgtctt taaaaaatca tacagctcgc gcggatcttt aaatggagtg tcttcttccc
    11521 agttttcgca atccacatcg gccagatcgt tattcagtaa gtaatccaat tcggctaagc
    11581 ggctgtctaa gctattcgta tagggacaat ccgatatgtc gatggagtga aagagcctga
    11641 tgcactccgc atacagctcg ataatctttt cagggctttg ttcatcttca tactcttccg
    11701 agcaaaggac gccatcggcc tcactcatga gcagattgct ccagccatca tgccgttcaa
    11761 agtgcaggac ctttggaaca ggcagctttc cttccagcca tagcatcatg tccttttccc
    11821 gttccacatc ataggtggtc cctttatacc ggctgtccgt catttttaaa tataggtttt
    11881 cattttctcc caccagctta tataccttag caggagacat tccttccgta tcttttacgc
    11941 agcggtattt ttcgatcagt tttttcaatt ccggtgatat tctcatttta gccatttatt
    12001 atttccttcc tcttttctac agtatttaaa gataccccaa gaagctaatt ataacaagac
    12061 gaactccaat tcactgttcc ttgcattcta aaaccttaaa taccagaaaa cagctttttc
    12121 aaagttgttt tcaaagttgg cgtataacat agtatcgacg gagccgattt tgaaaccgcg
    12181 gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc tccgcgagat
    12241 catccgtgtt tcaaacccgg cagcttagtt gccgttcttc cgaatagcat cggtaacatg
    12301 agcaaagtct gccgccttac aacggctctc ccgctgacgc cgtcccggac tgatgggctg
    12361 cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg gctggctggt
    12421 ggcaggatat attgtggtgt aaacaaattg acgcttagac aacttaataa cacattgcgg
    12481 acgtttttaa tgtactgaat taacgccgaa ttaattccta ggccaccatg ttgggcccgg
    12541 cgcgcc
//
