LOCUS       sequence-502179-        9359 bp    DNA     circular SYN 09-MAR-2026
DEFINITION  Expresses DOX-suppressible RELA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9359)
  TITLE     Targeting DNA polymerase epsilon leads to tumor clearance and 
            activation of an NF-_B-mediated inflammatory response in triple 
            negative breast cancer
REFERENCE   2  (bases 1 to 9359)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 9, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCW57.1 DOX off puro RELA
FEATURES             Location/Qualifiers
     source          1..9359
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    41..158
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        212..526
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    220..238
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    256..274
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    291..309
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    327..345
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    363..381
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    398..416
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    434..452
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     499..523
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             546..611
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             1908..2279
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     promoter        2301..2811
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2730..2748
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2821..3417
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2821..2840)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3317..3337
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             3418..3471
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(3545..3564)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     CDS             4099..4209
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
     primer_bind     complement(4258..4278)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4676..4687)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4892..5125
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5197..5318
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5234..5253)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5288..5307
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5358..5493
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        5493..5564
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5565..5633,5634..6425)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5783..5802)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6596..7184
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7085..7104
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7338..7355
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7472..7493
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7508..7513,7514..7531,7532..7538)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7546..7562
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7551..7573
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7570..7586
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7570..7586
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7605..7625
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7607..7625
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7653..7879
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7880..8060
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8107..8232
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8725..8958
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9143..9187
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 aattctcgac ctcgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
       61 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
      121 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
      181 gagatccact ttggccgcga atcgatatgt cgagtttact ccctatcagt gatagagaac
      241 gtatgtcgag tttactccct atcagtgata gagaacgatg tcgagtttac tccctatcag
      301 tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt
      361 actccctatc agtgatagag aacgtatgtc gagtttatcc ctatcagtga tagagaacgt
      421 atgtcgagtt tactccctat cagtgataga gaacgtatgt cgaggtaggc gtgtacggtg
      481 ggaggcctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gaattggcta
      541 gcatggacta taaggatcac gatggtgact acaaggacca cgacatcgac tacaaggacg
      601 acgacgacaa gggaggcggt ggaggcatgg acgaactgtt ccccctcatc ttcccggcag
      661 agccagccca ggcctctggc ccctatgtgg agataatcga acaaccgaag cagcgcggca
      721 tgcgcttccg ctacaagtgc gaggggcgct ccgcgggcag catcccaggc gagaggagca
      781 cagataccac caagacccac cccaccatca agatcaatgg ctacacagga ccagggacag
      841 tgcgcatctc cctggtcacc aaggaccctc ctcaccggcc tcacccccac gagcttgtag
      901 gaaaggactg ccgggatggc ttctatgagg ctgagctctg cccggaccgc tgcatccaca
      961 gtttccagaa cctgggaatc cagtgtgtga agaagcgaga tctcgaacaa gctatcagtc
     1021 agcgcatcca gaccaacaac aaccccttcc aagttcctat agaagagcag cgtggggact
     1081 acgacctgaa tgctgtgcgg ctctgcttcc aggtgacagt gcgggaccca tcaggcaggc
     1141 ccctccgcct gccgcctgtc ctttctcatc ccatctttga caatcgtgcc cccaacactg
     1201 ccgagctcaa gatctgccga gtgaaccgaa actctggcag ctgcctcggt ggggatgaga
     1261 tcttcctact gtgtgacaag gtgcagaaag aggacattga ggtgtatttc acgggaccag
     1321 gctgggaggc ccgaggctcc ttttcgcaag ctgatgtgca ccgacaagtg gccattgtgt
     1381 tccggacccc tccctacgca gaccccagcc tgcaggctcc tgtgcgtgtc tccatgcagc
     1441 tgcggcggcc ttccgaccgg gagctcagtg agcccatgga gttccagtac ctgccagata
     1501 cagacgatcg tcaccggatt gaggagaaac gtaaaaggac atatgagacc ttcaagagca
     1561 tcatgaagaa gagtcctttc agcggaccca ccgacccccg gcctccacct cgacgcattg
     1621 ctgtgccttc ccgcagctca gcttctgtcc ccaagccagc accccagccc tatcccttta
     1681 cgtcatccct gagcaccatc aactatgatg agtttcccac catggtgttt ccttctgggc
     1741 agatcagcca ggctagtgct ttggctccag cacctccaca agtcctgccc caggctccag
     1801 cccctgcccc tgctccagcc atggtatcag ctctggccca ggccccagct cctgtaccag
     1861 ttctagcacc aggacctcct caagctgtgg ctccacctgc ccccaagccc acccaggctg
     1921 gggaaggaac gctgtcagag gccctgctgc agctgcagtt tgatgatgaa gacctggggg
     1981 ccttgcttgg caacagcaca gacccagctg tgttcacaga cctggcatcc gtggacaact
     2041 ccgagtttca gcagctgctg aaccagggca tacctgtggc cccccacaca actgagccca
     2101 tgctgatgga gtaccctgag gctataactc gcctagtgac aggggcccag aggccccccg
     2161 acccagctcc tgctccactg ggggccccgg ggctccccaa tggcctcctt tcaggagatg
     2221 aagacttctc ctccattgcg gacatggact tctcagccct gctgagtcag atcagctcct
     2281 aggtcgacga attcggatcc ggggttgggg ttgcgccttt tccaaggcag ccctgggttt
     2341 gcgcagggac gcggctgctc tgggcgtggt tccgggaaac gcagcggcgc cgaccctggg
     2401 tctcgcacat tcttcacgtc cgttcgcagc gtcacccgga tcttcgccgc tacccttgtg
     2461 ggccccccgg cgacgcttcc tgctccgccc ctaagtcggg aaggttcctt gcggttcgcg
     2521 gcgtgccgga cgtgacaaac ggaagccgca cgtctcacta gtaccctcgc agacggacag
     2581 cgccagggag caatggcagc gcgccgaccg cgatgggctg tggccaatag cggctgctca
     2641 gcagggcgcg ccgagagcag cggccgggaa ggggcggtgc gggaggcggg gtgtggggcg
     2701 gtagtgtggg ccctgttcct gcccgcgcgg tgttccgcat tctgcaagcc tccggagcgc
     2761 acgtcggcag tcggctccct cgttgaccga atcaccgacc tctctcccca gcaattcacc
     2821 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta
     2881 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac
     2941 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac
     3001 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag
     3061 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt
     3121 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag
     3181 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc
     3241 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg
     3301 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc
     3361 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgccgaa
     3421 ggtagaggtt ctctcctcac ttgtggtgat gttgaagaaa accctggtcc aatgtctaga
     3481 ttagataaaa gtaaagtgat taacagcgca ttagagctgc ttaatgaggt cggaatcgaa
     3541 ggtttaacaa cccgtaaact cgcccagaag ctaggtgtag agcagcctac attgtattgg
     3601 catgtaaaaa ataagcgggc tttgctcgac gccttagcca ttgagatgtt agataggcac
     3661 catactcact tttgcccttt agaaggggaa agctggcaag attttttacg taataacgct
     3721 aaaagtttta gatgtgcttt actaagtcat cgcgatggag caaaagtaca tttaggtaca
     3781 cggcctacag aaaaacagta tgaaactctc gaaaatcaat tagccttttt atgccaacaa
     3841 ggtttttcac tagagaatgc attatatgca ctcagcgctg tggggcattt tactttaggt
     3901 tgcgtattgg aagatcaaga gcatcaagtc gctaaagaag aaagggaaac acctactact
     3961 gatagtatgc cgccattatt acgacaagct atcgaattat ttgatcacca aggtgcagag
     4021 ccagccttct tattcggcct tgaattgatc atatgtggcc tggagaaaca gctaaagtgc
     4081 gaaagcggcg ggccgaccga cgcccttgac gattttgact tagacatgct cccagccgat
     4141 gcccttgacg actttgacct tgatatgctg cctgctgacg ctcttgacga ttttgacctt
     4201 gacatgctcc ccgggtaact aagtaaggat cgatccaaga tatcgtattc ttaactatgt
     4261 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     4321 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     4381 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     4441 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     4501 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     4561 gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct
     4621 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     4681 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     4741 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctgtttc
     4801 gcctcggcgt ccggactaga ggtaccttta agaccaatga cttacaaggc agctgtagat
     4861 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga
     4921 caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag
     4981 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     5041 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     5101 tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta
     5161 taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat
     5221 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     5281 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc
     5341 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     5401 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     5461 tccagaagta gtgaggaggc ttttttggag gcctttcaaa tatgtatccg ctcatgagac
     5521 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     5581 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     5641 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     5701 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     5761 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     5821 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     5881 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     5941 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     6001 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     6061 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     6121 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     6181 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     6241 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     6301 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     6361 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     6421 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     6481 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     6541 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     6601 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     6661 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     6721 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     6781 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     6841 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     6901 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     6961 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     7021 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     7081 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     7141 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     7201 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     7261 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     7321 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     7381 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     7441 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     7501 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     7561 aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact
     7621 aaagggaaca aaagctggag ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc
     7681 ttgcaacatg gtaacgatga gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc
     7741 atgccgattg gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc aacagacggg
     7801 tctgacatgg attggacgaa ccactgaatt gccgcattgc agagatattg tatttaagtg
     7861 cctagctcga tacataaacg ggtctctctg gttagaccag atctgagcct gggagctctc
     7921 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     7981 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     8041 agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca
     8101 gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg
     8161 cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg
     8221 tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta
     8281 aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta
     8341 gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg
     8401 ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca
     8461 gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta
     8521 gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccgctgat
     8581 cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa
     8641 agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca
     8701 gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg
     8761 aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg
     8821 tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca
     8881 actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct
     8941 aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc
     9001 tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac
     9061 ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga
     9121 agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc
     9181 aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat
     9241 gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag
     9301 agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggac
//
