LOCUS       sequence-502241-        4934 bp    DNA     circular SYN 10-MAR-2026
DEFINITION  AiE2218m is an enhancer sequence, designed to drive AAV-mediated 
            transgene expression in specific populations of brain cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4934)
  TITLE     Allen Institute AAV Enhancer Plasmids
REFERENCE   2  (bases 1 to 4934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 10, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: AiP1551 - pAAV-AiE2218m-minBG-SYFP2-WPRE3-BGHpA
FEATURES             Location/Qualifiers
     source          1..4934
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      361..370
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(367..369,370..372,373..1083)
                     /codon_start=1
                     /product="monomeric optimized variant of EYFP, identical to
                     mVenus but with L68V (Kremers et al., 2006)"
                     /label=SYFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(412..433)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(673..692)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1020..1041
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1176..1196)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    1383..1494
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   1612..1752
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   1612..1741
                     /label=AAV2 ITR
     rep_origin      1827..2282
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1914..1933)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2124..2145
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2299..2318)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2418..2440
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2478..2496)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2564..2668
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2669..2737,2738..3529)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2887..2906)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3700..4288
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4189..4208
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   4350..4479
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
ORIGIN
        1 gcatatcaaa aattgatgag ctgccagcag cagtccctat gacattaatt caccaattgt
       61 gtttctaata gtttcatcct taaataaatt tggatgatga gaatggtaag gagagtcaag
      121 gttctgaaaa caatgaacag ggccaggcca gtgttcagtt tttatacagt agagaggagt
      181 gtagcggtgg taaatgttaa tggcaggcca ggcctgctgt actctgggtt attagccagt
      241 gcaagacaaa gatgcattgc gccggcggag ctcgggctgg gcataaaagt cagggcagag
      301 ccatctattg cttacatttg cttctgggat ccagatcttt cgaagctagc gctaccggtc
      361 gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
      421 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
      481 acctacggca agctgaccct gaagctgatc tgcaccaccg gcaagctgcc cgtgccctgg
      541 cccaccctcg tgaccaccct gggctacggc gtgcagtgct tcgcccgcta ccccgaccac
      601 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
      661 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
      721 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
      781 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcaccgc cgacaagcag
      841 aagaacggca tcaaggccaa cttcaagatc cgccacaaca tcgaggacgg cggcgtgcag
      901 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
      961 aaccactacc tgagctacca gtccaagctg agcaaagacc ccaacgagaa gcgcgatcac
     1021 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
     1081 aagtaagtcg acggcgcgcc gcggccgcga attcgatatc ataatcaacc tctggattac
     1141 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     1201 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     1261 tccttgtata aatcctggtt agttcttgcc acggcggaac tcatcgccgc ctgccttgcc
     1321 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggctcg agagatcttc
     1381 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     1441 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     1501 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     1561 ttgggaagac aatagcaggc atgagatctc acgtgcggac cgagcggccg caggaacccc
     1621 tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag gccgggcgac
     1681 caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc agtgagcgag cgagcgcgca
     1741 gctgcctgca ggggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     1801 accgcatacg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa gcgcggcggg
     1861 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccttagcgc ccgctccttt
     1921 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     1981 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     2041 tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     2101 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaactc
     2161 tatctcgggc tattcttttg atttataagg gattttgccg atttcggtct attggttaaa
     2221 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat
     2281 tttatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca
     2341 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
     2401 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
     2461 acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     2521 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     2581 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     2641 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     2701 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     2761 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     2821 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     2881 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     2941 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     3001 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     3061 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     3121 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     3181 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     3241 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     3301 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     3361 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     3421 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     3481 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     3541 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     3601 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     3661 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     3721 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     3781 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     3841 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     3901 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     3961 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     4021 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     4081 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     4141 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     4201 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     4261 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     4321 ggccttttgc tggccttttg ctcacatgtc ctgcaggcag ctgcgcgctc gctcgctcac
     4381 tgaggccgcc cgggcgtcgg gcgacctttg gtcgcccggc ctcagtgagc gagcgagcgc
     4441 gcagagaggg agtggccaac tccatcacta ggggttcctg cggccgcacg cgttgtaaga
     4501 gtaataatcg ccggcgattt aaagtaaatt aaacttaatg tgcaggttat tgcaattaag
     4561 tctgccttgt ctttctgagg gatttcaaca tcctcagtat ttaagaggca ctcataatgc
     4621 atgcaagtgg gcgggctttt tggctaatta acacaagagt ctaatctgct tttcaattag
     4681 gatggatgtt tataatgtgc gggtgacagt gttgcgatcc catgcagcct aacaggagtg
     4741 cacagcataa ttatagctga aaagagaggc tcatgcaggg cctttttagg ggttaatgag
     4801 acatcggcag cttccagctg aggagatcga gccagtttag tgtagcttca agaggaaacc
     4861 acccataggg gtttcctttg aagtgtcaaa aggtcccctt gttctgggtg gcttatgttt
     4921 catttgtctg agtg
//
