LOCUS       sequence-502254-        5226 bp    DNA     circular SYN 10-MAR-2026
DEFINITION  AiE2238m is an enhancer sequence, designed to drive AAV-mediated 
            transgene expression in specific populations of brain cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5226)
  TITLE     Allen Institute AAV Enhancer Plasmids
REFERENCE   2  (bases 1 to 5226)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 10, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: AiP1571 - pAAV-AiE2238m-minBG-SYFP2-WPRE3-BGHpA
FEATURES             Location/Qualifiers
     source          1..5226
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(481..1272,1273..1341))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1104..1123
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1342..1446)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1514..1532
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1570..1592)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1692..1711
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(1728..2183)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1865..1886)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2077..2096
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     repeat_region   2258..2398
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   2269..2398
                     /label=AAV2 ITR
     polyA_signal    2516..2627
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     2814..2834
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(join(2927..3637,3638..3640,3641..3643))
                     /codon_start=1
                     /product="monomeric optimized variant of EYFP, identical to
                     mVenus but with L68V (Kremers et al., 2006)"
                     /label=SYFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2969..2990)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     3318..3337
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3577..3598
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      3640..3649
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     repeat_region   4757..4886
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     rep_origin      complement(join(4948..5226,1..310))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5028..5047)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
       61 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
      121 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
      181 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
      241 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
      301 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
      361 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
      421 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
      481 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
      541 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
      601 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
      661 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
      721 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
      781 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
      841 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
      901 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
      961 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     1021 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     1081 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     1141 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     1201 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     1261 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     1321 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     1381 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     1441 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
     1501 attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga
     1561 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
     1621 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg
     1681 gcttaactat gcggcatcag agcagattgt actgagagtg caccataaaa ttgtaaacgt
     1741 taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata
     1801 gaccgaaatc ggcaaaatcc cttataaatc aaaagaatag cccgagatag agttgagtgt
     1861 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg
     1921 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcacccaaat caagtttttt
     1981 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc
     2041 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg
     2101 cgctaaggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct
     2161 taatgcgccg ctacagggcg cgtactatgg ttgctttgac gtatgcggtg tgaaataccg
     2221 cacagatgcg taaggagaaa ataccgcatc aggcgcccct gcaggcagct gcgcgctcgc
     2281 tcgctcactg aggccgcccg ggcaaagccc gggcgtcggg cgacctttgg tcgcccggcc
     2341 tcagtgagcg agcgagcgcg cagagaggga gtggccaact ccatcactag gggttcctgc
     2401 ggccgctcgg tccgcacgtg agatctcatg cctgctattg tcttcccaat cctccccctt
     2461 gctgtcctgc cccaccccac cccccagaat agaatgacac ctactcagac aatgcgatgc
     2521 aatttcctca ttttattagg aaaggacagt gggagtggca ccttccaggg tcaaggaagg
     2581 cacgggggag gggcaaacaa cagatggctg gcaactagaa ggcacagtcg aagatctctc
     2641 gagccacgga attgtcagtg cccaacagcc gagcccctgt ccagcagcgg gcaaggcagg
     2701 cggcgatgag ttccgccgtg gcaagaacta accaggattt atacaaggag gagaaaatga
     2761 aagccatacg ggaagcaata gcatgataca aaggcattaa agcagcgtat ccacatagcg
     2821 taaaaggagc aacatagtta agaataccag tcaatctttc acaaattttg taatccagag
     2881 gttgattatg atatcgaatt cgcggccgcg gcgcgccgtc gacttacttg tacagctcgt
     2941 ccatgccgag agtgatcccg gcggcggtca cgaactccag caggaccatg tgatcgcgct
     3001 tctcgttggg gtctttgctc agcttggact ggtagctcag gtagtggttg tcgggcagca
     3061 gcacggggcc gtcgccgatg ggggtgttct gctggtagtg gtcggcgagc tgcacgccgc
     3121 cgtcctcgat gttgtggcgg atcttgaagt tggccttgat gccgttcttc tgcttgtcgg
     3181 cggtgatata gacgttgtgg ctgttgtagt tgtactccag cttgtgcccc aggatgttgc
     3241 cgtcctcctt gaagtcgatg cccttcagct cgatgcggtt caccagggtg tcgccctcga
     3301 acttcacctc ggcgcgggtc ttgtagttgc cgtcgtcctt gaagaagatg gtgcgctcct
     3361 ggacgtagcc ttcgggcatg gcggacttga agaagtcgtg ctgcttcatg tggtcggggt
     3421 agcgggcgaa gcactgcacg ccgtagccca gggtggtcac gagggtgggc cagggcacgg
     3481 gcagcttgcc ggtggtgcag atcagcttca gggtcagctt gccgtaggtg gcatcgccct
     3541 cgccctcgcc ggacacgctg aacttgtggc cgtttacgtc gccgtccagc tcgaccagga
     3601 tgggcaccac cccggtgaac agctcctcgc ccttgctcac catggtggcg accggtagcg
     3661 ctagcttcga aagatctgga tcccagaagc aaatgtaagc aatagatggc tctgccctga
     3721 cttttatgcc cagcccgagc tccgccggcg gcagtgtcag tcaaagccaa gctgctgaga
     3781 cactacagct gccacggctc aagcagacac agggcacgta cccttcatcc cctgtaccac
     3841 ctgatcagat agatgactca gtggccatga ctactgaatg tgggtgggtc aggcaggacc
     3901 ctaacacctc agggttcccc aaattggaat gcgccaggct gtcaagagta acaagaggag
     3961 ggacttgttc ctctctggtg cttttcctgg ggtcaaacga gacatctcat caccaattac
     4021 cgtaaagcca ggttggaagg aaggagagag ggagtaactt cagggagata attggacaga
     4081 tgagagcttt cagtcccttt aactaaccct ggaactcaca ttgactttaa aggaagagaa
     4141 tatatatata tatataaaat agttgtttca aaagaatatt gaggatgaaa tatcacttac
     4201 ataaggggaa gatagtgcct gttcatctta attagctgga agtcagtcct gtaccacagg
     4261 ggcatcacac cacgtgggtg gatccacact gtgctggaaa gagcaatagc agcgcctgtc
     4321 agtcccctgt actcatctca gggggcgtta gggccctgct tgctggtgct gcagatcaag
     4381 cttgttaaga taaagttggg cgggtgcttg caatcacttg caaagcctcc ccctgccgca
     4441 ggcctgccca cttttcctga ctcagaggtc tatgttcttt ttcctcgtcc atttctttcc
     4501 gatgtcagtc aaagcctgtg cgtgctgccc ctgacactct aggcctcatg tgttctctga
     4561 agacttactt catcctccac ccacttctgc cttgctgctg ctagccaagc tccagggctg
     4621 agcccagcag gctttcttgt atcctaacct aaaaggtgtg attttctttc ttcctctctt
     4681 tcaggcaact gccgcagtct acagcctgag tgccctgctc gccggcgatt attactctta
     4741 caacgcgtgc ggccgcagga acccctagtg atggagttgg ccactccctc tctgcgcgct
     4801 cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac gcccgggcgg cctcagtgag
     4861 cgagcgagcg cgcagctgcc tgcaggacat gtgagcaaaa ggccagcaaa aggccaggaa
     4921 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     4981 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     5041 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     5101 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     5161 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     5221 gcccga
//
