LOCUS       sequence-502272-        5139 bp    DNA     circular SYN 10-MAR-2026
DEFINITION  AiE2260m is an enhancer sequence, designed to drive AAV-mediated 
            transgene expression in specific populations of brain cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5139)
  TITLE     Allen Institute AAV Enhancer Plasmids
REFERENCE   2  (bases 1 to 5139)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 10, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: AiP1593 - pAAV-AiE2260m-minBG-SYFP2-WPRE3-BGHpA
FEATURES             Location/Qualifiers
     source          1..5139
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   130..259
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     regulatory      1280..1289
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1286..1288,1289..1291,1292..2002)
                     /codon_start=1
                     /product="monomeric optimized variant of EYFP, identical to
                     mVenus but with L68V (Kremers et al., 2006)"
                     /label=SYFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1331..1352)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1592..1611)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1939..1960
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(2095..2115)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    2302..2413
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   2531..2671
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   2531..2660
                     /label=AAV2 ITR
     rep_origin      2746..3201
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2833..2852)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3043..3064
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3218..3237)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3337..3359
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3397..3415)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3483..3587
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3588..3656,3657..4448)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3806..3825)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(4619..5139,1..68)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5108..5127
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
       61 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
      121 ctcacatgtc ctgcaggcag ctgcgcgctc gctcgctcac tgaggccgcc cgggcgtcgg
      181 gcgacctttg gtcgcccggc ctcagtgagc gagcgagcgc gcagagaggg agtggccaac
      241 tccatcacta ggggttcctg cggccgcacg cgttgtaaga gtaataatcg ccggcgattg
      301 cagcactgca tgaaaggaca gaaggttaaa agtgtggtgt gtgtgtgtgt gtgtgtgtgt
      361 gtgtgtgtgt gtgtgtgaag ctggtggttg gtacctttac ctgctgagtc atttctctgg
      421 tctatctgcc tatgtttttt ctaaagtgga tttcccagcc ttttttcttc agtccttagg
      481 aagacactgc aaggtcattg ttgtcccttt tattcaagag tcacatgagc tcagcctggg
      541 tcagcacctt gctcaggctc tcagaattgg gagcattaac ctcatctgtc ctcttctgac
      601 ttctacactt tgcatttagc taatgtgtac ttctgtgttc tcccttgcat caaatcctta
      661 tccaaagatg ctcatcagcc aggataaatg attcaccaag gaaagaggaa acccttccta
      721 aaaccacctc aggtgcaagg agtgagagtt ggggactagg ggtagggagg tgggaaggtg
      781 attgtagagg ttaggaggga aggacaggaa gagaaagaaa ggagatggag accatgagga
      841 aagccaggga ggcagcaatt gcagaagagg aaactcacac ttgattttcg actagagctg
      901 aaaggagaga agagggagga agctgaggag actcaggacc agcagcaggg aacaaagctc
      961 tgagagcatc ctggtgctcc ctacctgtcc ccctcctcgg gtctccctgt gcagcctgag
     1021 tcatccccgt tactcctcca tcccaggcca gaggctcctc cctaaaaccc catcccactc
     1081 aggggcttcc acagcccacc ctcgcaaggc ctggaaagtt catctgtggg tctgatttgc
     1141 tactcatttt aatgaggaag aaattccctt taggaacacg ccggcggagc tcgggctggg
     1201 cataaaagtc agggcagagc catctattgc ttacatttgc ttctgggatc cagatctttc
     1261 gaagctagcg ctaccggtcg ccaccatggt gagcaagggc gaggagctgt tcaccggggt
     1321 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
     1381 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagctgatct gcaccaccgg
     1441 caagctgccc gtgccctggc ccaccctcgt gaccaccctg ggctacggcg tgcagtgctt
     1501 cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     1561 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     1621 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     1681 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
     1741 tatcaccgcc gacaagcaga agaacggcat caaggccaac ttcaagatcc gccacaacat
     1801 cgaggacggc ggcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     1861 ccccgtgctg ctgcccgaca accactacct gagctaccag tccaagctga gcaaagaccc
     1921 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     1981 cggcatggac gagctgtaca agtaagtcga cggcgcgccg cggccgcgaa ttcgatatca
     2041 taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     2101 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     2161 tatggctttc attttctcct ccttgtataa atcctggtta gttcttgcca cggcggaact
     2221 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     2281 cgtggctcga gagatcttcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
     2341 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
     2401 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
     2461 aggacagcaa gggggaggat tgggaagaca atagcaggca tgagatctca cgtgcggacc
     2521 gagcggccgc aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg
     2581 ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca
     2641 gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga tgcggtattt tctccttacg
     2701 catctgtgcg gtatttcaca ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg
     2761 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     2821 ccttagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     2881 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     2941 tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga
     3001 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     3061 ctggaacaac actcaactct atctcgggct attcttttga tttataaggg attttgccga
     3121 tttcggtcta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     3181 aaatattaac gtttacaatt ttatggtgca ctctcagtac aatctgctct gatgccgcat
     3241 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     3301 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     3361 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
     3421 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     3481 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     3541 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     3601 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     3661 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     3721 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     3781 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     3841 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     3901 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     3961 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     4021 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     4081 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     4141 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     4201 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     4261 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     4321 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     4381 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     4441 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     4501 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     4561 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     4621 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     4681 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     4741 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     4801 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     4861 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     4921 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     4981 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     5041 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     5101 ttccaggggg aaacgcctgg tatctttata gtcctgtcg
//
