LOCUS       sequence-502275-        5258 bp    DNA     circular SYN 10-MAR-2026
DEFINITION  AiE2263m is an enhancer sequence, designed to drive AAV-mediated 
            transgene expression in specific populations of brain cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5258)
  TITLE     Allen Institute AAV Enhancer Plasmids
REFERENCE   2  (bases 1 to 5258)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 10, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: AiP1596 - pAAV-AiE2263m-minBG-SYFP2-WPRE3-BGHpA
FEATURES             Location/Qualifiers
     source          1..5258
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   86..226
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   97..226
                     /label=AAV2 ITR
     polyA_signal    344..455
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     642..662
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(join(755..1465,1466..1468,1469..1471))
                     /codon_start=1
                     /product="monomeric optimized variant of EYFP, identical to
                     mVenus but with L68V (Kremers et al., 2006)"
                     /label=SYFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(797..818)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     1146..1165
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1405..1426
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      1468..1477
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     repeat_region   2617..2746
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     rep_origin      complement(2808..3396)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2888..2907)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3567..4358,4359..4427))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4190..4209
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4428..4532)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4600..4618
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4656..4678)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4778..4797
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(join(4814..5258,1..11))
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4951..4972)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5163..5182
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 acagggcgcg tactatggtt gctttgacgt atgcggtgtg aaataccgca cagatgcgta
       61 aggagaaaat accgcatcag gcgcccctgc aggcagctgc gcgctcgctc gctcactgag
      121 gccgcccggg caaagcccgg gcgtcgggcg acctttggtc gcccggcctc agtgagcgag
      181 cgagcgcgca gagagggagt ggccaactcc atcactaggg gttcctgcgg ccgctcggtc
      241 cgcacgtgag atctcatgcc tgctattgtc ttcccaatcc tcccccttgc tgtcctgccc
      301 caccccaccc cccagaatag aatgacacct actcagacaa tgcgatgcaa tttcctcatt
      361 ttattaggaa aggacagtgg gagtggcacc ttccagggtc aaggaaggca cgggggaggg
      421 gcaaacaaca gatggctggc aactagaagg cacagtcgaa gatctctcga gccacggaat
      481 tgtcagtgcc caacagccga gcccctgtcc agcagcgggc aaggcaggcg gcgatgagtt
      541 ccgccgtggc aagaactaac caggatttat acaaggagga gaaaatgaaa gccatacggg
      601 aagcaatagc atgatacaaa ggcattaaag cagcgtatcc acatagcgta aaaggagcaa
      661 catagttaag aataccagtc aatctttcac aaattttgta atccagaggt tgattatgat
      721 atcgaattcg cggccgcggc gcgccgtcga cttacttgta cagctcgtcc atgccgagag
      781 tgatcccggc ggcggtcacg aactccagca ggaccatgtg atcgcgcttc tcgttggggt
      841 ctttgctcag cttggactgg tagctcaggt agtggttgtc gggcagcagc acggggccgt
      901 cgccgatggg ggtgttctgc tggtagtggt cggcgagctg cacgccgccg tcctcgatgt
      961 tgtggcggat cttgaagttg gccttgatgc cgttcttctg cttgtcggcg gtgatataga
     1021 cgttgtggct gttgtagttg tactccagct tgtgccccag gatgttgccg tcctccttga
     1081 agtcgatgcc cttcagctcg atgcggttca ccagggtgtc gccctcgaac ttcacctcgg
     1141 cgcgggtctt gtagttgccg tcgtccttga agaagatggt gcgctcctgg acgtagcctt
     1201 cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg gtcggggtag cgggcgaagc
     1261 actgcacgcc gtagcccagg gtggtcacga gggtgggcca gggcacgggc agcttgccgg
     1321 tggtgcagat cagcttcagg gtcagcttgc cgtaggtggc atcgccctcg ccctcgccgg
     1381 acacgctgaa cttgtggccg tttacgtcgc cgtccagctc gaccaggatg ggcaccaccc
     1441 cggtgaacag ctcctcgccc ttgctcacca tggtggcgac cggtagcgct agcttcgaaa
     1501 gatctggatc ccagaagcaa atgtaagcaa tagatggctc tgccctgact tttatgccca
     1561 gcccgagctc cgccggcgag agaagccggg agcgtgtagc agcggcggat aaatattcat
     1621 taggaagtgt aagtcctttc aaagggctcc gcgcgcgcgc tctagctcct gtctctcttt
     1681 ctctccctcc ctctgtcctc caggaaggct tcccctcctc tagctatctc aacgcccccc
     1741 cccccttcct gcgaaatacc gggtggatcc taggctagct aacctgttgg gaagcctgaa
     1801 ctgcggtagc gcctctgcag ctagcagttt gctttcgctc ctggggcgcg cgtccccccc
     1861 catcccctcc cctccttcct ttcgcccagc ccctcacccc tcctaaatca ctacatcatt
     1921 agctttattg ggcggggggg gggcgggtaa cggagcagcc agacgcacct ctccatagaa
     1981 gggtgaatag ttggtgccct gcgatgcaac cccaactcca tctccacctc attctcaccc
     2041 atctgcacat cctcatccaa tcttgcttct ttttcttcgc tctttccacc tccacctcca
     2101 tcctgtgtca acctaactcc ctcagcagcc ctcccacccg ccgccgccaa tccggatcac
     2161 agattgggtt gggagtgaga ttgcaaggct gttaggtccg aaggtcctgt gtgtcgaggt
     2221 tagaactcct ccctggcgtg agtacggggt ggttatcaag atctccttct ccttcctgac
     2281 cctgcaaggt taccaaacag cctggacttt tgaatcagat ctctggagca agcattaaga
     2341 actccagcca cacagatctc agggcctggc cctttcactc agccgcggag gctgcttgag
     2401 ggatggggag aggtgatttc actcagcgcc aacgcgcagg aaaaactcat agacgacagt
     2461 cggactaata ctcgtccctt aagaatgcac tacctgaagt tagtcgcaag tcatcttcaa
     2521 tcgaggaaag gaaaaaaaaa atcctcacat ctagaagctt cgccgaagcc acttccaacc
     2581 gccggcgatt attactctta caacgcgtgc ggccgcagga acccctagtg atggagttgg
     2641 ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac
     2701 gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc tgcaggacat gtgagcaaaa
     2761 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     2821 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     2881 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     2941 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     3001 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     3061 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     3121 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     3181 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     3241 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     3301 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     3361 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     3421 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     3481 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     3541 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     3601 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     3661 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     3721 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     3781 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     3841 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     3901 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     3961 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     4021 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     4081 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     4141 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     4201 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     4261 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     4321 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     4381 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     4441 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     4501 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     4561 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     4621 tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag
     4681 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
     4741 gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg
     4801 agagtgcacc ataaaattgt aaacgttaat attttgttaa aattcgcgtt aaatttttgt
     4861 taaatcagct cattttttaa ccaatagacc gaaatcggca aaatccctta taaatcaaaa
     4921 gaatagcccg agatagagtt gagtgttgtt ccagtttgga acaagagtcc actattaaag
     4981 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt
     5041 gaaccatcac ccaaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac
     5101 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag
     5161 gaagggaaga aagcgaaagg agcgggcgct aaggcgctgg caagtgtagc ggtcacgctg
     5221 cgcgtaacca ccacacccgc cgcgcttaat gcgccgct
//
