LOCUS       sequence-502367-        5104 bp    DNA     circular SYN 10-MAR-2026
DEFINITION  AiE2246m is an enhancer sequence, designed to drive AAV-mediated 
            transgene expression in specific populations of brain cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5104)
  TITLE     Allen Institute AAV Enhancer Plasmids
REFERENCE   2  (bases 1 to 5104)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 10, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: AiP1790 - pAAV-AiE2246m-minBG-iCre(R297T)-BGHpA
FEATURES             Location/Qualifiers
     source          1..5104
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   414..543
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     rep_origin      complement(605..1193)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(685..704)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1364..2155,2156..2224))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1987..2006
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2225..2329)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2397..2415
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2453..2475)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2575..2594
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(2611..3066)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2748..2769)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2960..2979
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     repeat_region   3141..3281
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3152..3281
                     /label=AAV2 ITR
     polyA_signal    3399..3510
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(4576..4596)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      4599..4608
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tcctgaaact ggtgtttatt ggttatcagg ttgtctcaga cgggagactt ccaccagctg
       61 aacaaatgac cttggccttg ccatctcatc cctgagcctc tgtgacccta tctgtggagt
      121 ggggtcatct ctgtgaagct ctcagagtca gtaggggcct gaccagcttg ttttcagatg
      181 gcagggcaca cggagatggc agtctcagtg aactatgaac aagttcacta acaagcatgc
      241 cacattttat tatgccaggg agtagggaga tggctctgga gcctgaggga gctctccagc
      301 tatggaggag gtcactcacc caggacatgt gtgcttataa attgtccggg acagaaatga
      361 agacatttga aagaaacgcc ggcgattatt actcttacaa cgcgtgcggc cgcaggaacc
      421 cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg
      481 accaaaggtc gcccgacgcc cgggcggcct cagtgagcga gcgagcgcgc agctgcctgc
      541 aggacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
      601 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
      661 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
      721 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
      781 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
      841 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
      901 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
      961 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     1021 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     1081 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     1141 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     1201 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     1261 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     1321 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     1381 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     1441 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     1501 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     1561 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     1621 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     1681 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     1741 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     1801 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     1861 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     1921 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     1981 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     2041 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     2101 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     2161 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     2221 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     2281 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     2341 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
     2401 ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac
     2461 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
     2521 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat
     2581 cagagcagat tgtactgaga gtgcaccata aaattgtaaa cgttaatatt ttgttaaaat
     2641 tcgcgttaaa tttttgttaa atcagctcat tttttaacca atagaccgaa atcggcaaaa
     2701 tcccttataa atcaaaagaa tagcccgaga tagagttgag tgttgttcca gtttggaaca
     2761 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
     2821 gcgatggccc actacgtgaa ccatcaccca aatcaagttt tttggggtcg aggtgccgta
     2881 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
     2941 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctaag gcgctggcaa
     3001 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
     3061 gcgcgtacta tggttgcttt gacgtatgcg gtgtgaaata ccgcacagat gcgtaaggag
     3121 aaaataccgc atcaggcgcc cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc
     3181 ccgggcaaag cccgggcgtc gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc
     3241 gcgcagagag ggagtggcca actccatcac taggggttcc tgcggccgct cggtccgcac
     3301 gtgagatctc atgcctgcta ttgtcttccc aatcctcccc cttgctgtcc tgccccaccc
     3361 caccccccag aatagaatga cacctactca gacaatgcga tgcaatttcc tcattttatt
     3421 aggaaaggac agtgggagtg gcaccttcca gggtcaagga aggcacgggg gaggggcaaa
     3481 caacagatgg ctggcaacta gaaggcacag tcgaagatct ctcgagaagc ttgaattcgg
     3541 cgcgcctcag tccccatctt cgagcagcct caccatggcc ccagtctcag agtccaggtt
     3601 tctgatgtag ttcatcacaa tgttcacatt ggtccagcca ccagcctgca tgatttcagg
     3661 gatggacaca ccagccctgg ccatgtcggt ggcagcaccc actctggcag agtggccaga
     3721 ccaggccagg tatctctgcc cagagtcatc cttggcacca tagatcaggc ggtgggtggc
     3781 ctcaaagatc ccttccaggg cccgggtgga cagttgggag gtggcagaag gggcagccac
     3841 accattcttt ctgacccggc agaacaggta gttgttgggg tcatcagcca caccagacac
     3901 agagatccat ctctccacca gcttggtaac ccccagggac agggccttct ccacaccagc
     3961 tgtggacacc agggtcttgg tcctgccaat gtggatcagc attctcccac catcggtgcg
     4021 ggagatgtcc ttcactctga ttctggcaat ttcggcaatg cgcagcaggg tgttgtaggc
     4081 aatgcccagg aaggccaggt tcctgatgtc ctggcatctg tcagagttct ccatcaggga
     4141 tctgacttgg tcaaagtcag tgcgttcaaa ggccagggcc tgcttggctc tctccccagc
     4201 atccacattc tcctttctga ttctcctcat caccagggac acagcattgg agtcagaagg
     4261 gcgaggcagg ccagatctcc tgtgcagcat gttgagctgg cccaggtgct gttggatggt
     4321 cttcacagcc aggcctctgg cttgcaggta caggaggtag tccctcacat cctcaggttc
     4381 agcagggaac catttcctgt tgttcagctt gcaccaggca gcccaggatc tgcacacaga
     4441 caggagcatc ttccaggtgt gttcagagaa ggcctgcctg tccctgaaca tgtccatcag
     4501 gttcttcctg acttcatcag aggtggcatc cacagggagg gcaggcaggt tttggtgcac
     4561 agtcagcagg ttggagactt tcctcttctt cttgggcacc atggtggcta gcttcgaaag
     4621 atctggatcc cagaagcaaa tgtaagcaat agatggctct gccctgactt ttatgcccag
     4681 cccgagctcc gccggcgaca tgacctgtga ccctgactcc actcaaactc ctgttctccc
     4741 tcctgatctt atgacccagg acaaaaggct gccccctgca aggaccctta gcactgcctc
     4801 ccagggattc cagtacaact cactgtgacc taactaaaaa ccagtctctg acagacattg
     4861 cgtgggtggc agaaacaaga gtgcagagag actgggccag ctgggcccgg aggtgtggtc
     4921 aggcccccag gggacaaagg cagcccagat gggaggtatc tacagagacc agcaccatct
     4981 agatcccagc agaacattaa tcaggtcatg gcctgagagg gacaaggcca gtccagaata
     5041 ggtttgtgtc tcctattaaa tgcatttcct ccctggagag ttgggacagt gcccagggat
     5101 cttg
//
