LOCUS       sequence-502368-        5016 bp    DNA     circular SYN 10-MAR-2026
DEFINITION  AiE2255m is an enhancer sequence, designed to drive AAV-mediated 
            transgene expression in specific populations of brain cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5016)
  TITLE     Allen Institute AAV Enhancer Plasmids
REFERENCE   2  (bases 1 to 5016)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 10, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: AiP1791 - pAAV-AiE2255m-minBG-iCre(R297T)-BGHpA
FEATURES             Location/Qualifiers
     source          1..5016
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(504..1295,1296..1364))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1127..1146
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1365..1469)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1537..1555
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1593..1615)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1715..1734
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(1751..2206)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1888..1909)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2100..2119
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     repeat_region   2281..2421
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   2292..2421
                     /label=AAV2 ITR
     polyA_signal    2539..2650
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(3716..3736)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      3739..3748
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     repeat_region   4570..4699
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     rep_origin      complement(join(4761..5016,1..333))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4841..4860)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
       61 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
      121 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
      181 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
      241 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
      301 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
      361 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
      421 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
      481 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
      541 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
      601 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
      661 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
      721 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
      781 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
      841 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
      901 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
      961 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     1021 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     1081 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     1141 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     1201 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     1261 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     1321 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     1381 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     1441 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
     1501 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
     1561 cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
     1621 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
     1681 ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga
     1741 gtgcaccata aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
     1801 atcagctcat tttttaacca atagaccgaa atcggcaaaa tcccttataa atcaaaagaa
     1861 tagcccgaga tagagttgag tgttgttcca gtttggaaca agagtccact attaaagaac
     1921 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
     1981 ccatcaccca aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
     2041 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
     2101 gggaagaaag cgaaaggagc gggcgctaag gcgctggcaa gtgtagcggt cacgctgcgc
     2161 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtacta tggttgcttt
     2221 gacgtatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
     2281 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
     2341 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
     2401 actccatcac taggggttcc tgcggccgct cggtccgcac gtgagatctc atgcctgcta
     2461 ttgtcttccc aatcctcccc cttgctgtcc tgccccaccc caccccccag aatagaatga
     2521 cacctactca gacaatgcga tgcaatttcc tcattttatt aggaaaggac agtgggagtg
     2581 gcaccttcca gggtcaagga aggcacgggg gaggggcaaa caacagatgg ctggcaacta
     2641 gaaggcacag tcgaagatct ctcgagaagc ttgaattcgg cgcgcctcag tccccatctt
     2701 cgagcagcct caccatggcc ccagtctcag agtccaggtt tctgatgtag ttcatcacaa
     2761 tgttcacatt ggtccagcca ccagcctgca tgatttcagg gatggacaca ccagccctgg
     2821 ccatgtcggt ggcagcaccc actctggcag agtggccaga ccaggccagg tatctctgcc
     2881 cagagtcatc cttggcacca tagatcaggc ggtgggtggc ctcaaagatc ccttccaggg
     2941 cccgggtgga cagttgggag gtggcagaag gggcagccac accattcttt ctgacccggc
     3001 agaacaggta gttgttgggg tcatcagcca caccagacac agagatccat ctctccacca
     3061 gcttggtaac ccccagggac agggccttct ccacaccagc tgtggacacc agggtcttgg
     3121 tcctgccaat gtggatcagc attctcccac catcggtgcg ggagatgtcc ttcactctga
     3181 ttctggcaat ttcggcaatg cgcagcaggg tgttgtaggc aatgcccagg aaggccaggt
     3241 tcctgatgtc ctggcatctg tcagagttct ccatcaggga tctgacttgg tcaaagtcag
     3301 tgcgttcaaa ggccagggcc tgcttggctc tctccccagc atccacattc tcctttctga
     3361 ttctcctcat caccagggac acagcattgg agtcagaagg gcgaggcagg ccagatctcc
     3421 tgtgcagcat gttgagctgg cccaggtgct gttggatggt cttcacagcc aggcctctgg
     3481 cttgcaggta caggaggtag tccctcacat cctcaggttc agcagggaac catttcctgt
     3541 tgttcagctt gcaccaggca gcccaggatc tgcacacaga caggagcatc ttccaggtgt
     3601 gttcagagaa ggcctgcctg tccctgaaca tgtccatcag gttcttcctg acttcatcag
     3661 aggtggcatc cacagggagg gcaggcaggt tttggtgcac agtcagcagg ttggagactt
     3721 tcctcttctt cttgggcacc atggtggcta gcttcgaaag atctggatcc cagaagcaaa
     3781 tgtaagcaat agatggctct gccctgactt ttatgcccag cccgagctcc gccggcgcct
     3841 cagagaagca gatcacacat ctgttcccat cagctccacc cttctgtaaa gacaaagaga
     3901 agctgccaag gccctgtgct tgccgagctt tgctctctga gaccccaagg aatcctacag
     3961 ggaagactgg aactggatga ctcaggacca catgggaaag atagctgggg atggcccctc
     4021 ttcccagcag ctcaggaatg ctgcccagct gagcccctgc ggagcacgtg gcttgtgccg
     4081 ccctgtggag ggtcctgcag agatgaggac gggtgcatgt ccccaccctg catggtctca
     4141 gaaatgcctg gtctcagcat cttcctctct gggtcagctg tttacagaga ggggagaggc
     4201 cacttcccca gaagctcaag cgctaagaca cctgcagggc agcaaatgag aaactcagcc
     4261 acctgcagat cctgctctac caatgccttc gttctggggg gataattggg caacgtggag
     4321 ccaagctcta acaatgtgag aacctctgat ctgctcattt gctctgagga ctgggtccca
     4381 gaaaacctgc tgccgaggca agggagccag agataacatc atccatccaa agatctaaac
     4441 cgtggcacct ttgactgaag gaggggagct agcaagcgga tacagggtgt gactcagtaa
     4501 actagttgat atggccttaa taaaacacag cccgccggcg attattactc ttacaacgcg
     4561 tgcggccgca ggaaccccta gtgatggagt tggccactcc ctctctgcgc gctcgctcgc
     4621 tcactgaggc cgggcgacca aaggtcgccc gacgcccggg cggcctcagt gagcgagcga
     4681 gcgcgcagct gcctgcagga catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     4741 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     4801 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     4861 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     4921 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     4981 tcggtgtagg tcgttcgctc caagctgggc tgtgtg
//
