LOCUS       sequence-502369-        5322 bp    DNA     circular SYN 10-MAR-2026
DEFINITION  AiE2253m is an enhancer sequence, designed to drive AAV-mediated 
            transgene expression in specific populations of brain cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5322)
  TITLE     Allen Institute AAV Enhancer Plasmids
REFERENCE   2  (bases 1 to 5322)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 10, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: AiP1998 - pAAV-AiE2253m-minBG-iCre(R297T)-BGHpA
FEATURES             Location/Qualifiers
     source          1..5322
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(927..947)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      950..959
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     repeat_region   2087..2216
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     rep_origin      complement(2278..2866)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2358..2377)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3037..3828,3829..3897))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3660..3679
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3898..4002)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4070..4088
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4126..4148)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4248..4267
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(4284..4739)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4421..4442)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4633..4652
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     repeat_region   4814..4954
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4825..4954
                     /label=AAV2 ITR
     polyA_signal    5072..5183
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 atgatttcag ggatggacac accagccctg gccatgtcgg tggcagcacc cactctggca
       61 gagtggccag accaggccag gtatctctgc ccagagtcat ccttggcacc atagatcagg
      121 cggtgggtgg cctcaaagat cccttccagg gcccgggtgg acagttggga ggtggcagaa
      181 ggggcagcca caccattctt tctgacccgg cagaacaggt agttgttggg gtcatcagcc
      241 acaccagaca cagagatcca tctctccacc agcttggtaa cccccaggga cagggccttc
      301 tccacaccag ctgtggacac cagggtcttg gtcctgccaa tgtggatcag cattctccca
      361 ccatcggtgc gggagatgtc cttcactctg attctggcaa tttcggcaat gcgcagcagg
      421 gtgttgtagg caatgcccag gaaggccagg ttcctgatgt cctggcatct gtcagagttc
      481 tccatcaggg atctgacttg gtcaaagtca gtgcgttcaa aggccagggc ctgcttggct
      541 ctctccccag catccacatt ctcctttctg attctcctca tcaccaggga cacagcattg
      601 gagtcagaag ggcgaggcag gccagatctc ctgtgcagca tgttgagctg gcccaggtgc
      661 tgttggatgg tcttcacagc caggcctctg gcttgcaggt acaggaggta gtccctcaca
      721 tcctcaggtt cagcagggaa ccatttcctg ttgttcagct tgcaccaggc agcccaggat
      781 ctgcacacag acaggagcat cttccaggtg tgttcagaga aggcctgcct gtccctgaac
      841 atgtccatca ggttcttcct gacttcatca gaggtggcat ccacagggag ggcaggcagg
      901 ttttggtgca cagtcagcag gttggagact ttcctcttct tcttgggcac catggtggct
      961 agcttcgaaa gatctggatc ccagaagcaa atgtaagcaa tagatggctc tgccctgact
     1021 tttatgccca gcccgagctc cgccggcgta ttatctattt atcaacaatc cacagatcag
     1081 gatgcaaact ctcagctact gcatcagtgc cagagctgcc tgcttacctg cctggtatca
     1141 tgcttcccac catgatattc atggactcac cctttgaaac tgtaaaaccc tcatcaaatt
     1201 ctctcttctg taagttacct tgattatgct gctttgtcat agcagtagaa aagtaacaaa
     1261 ggcattgtgt ctgttgatta atacctctgc tggtggccaa cagaccactc tgctggtggc
     1321 ccatctagcc catcattagg atgatgtgtt ctcttaacca ccctgagatt cagctccctt
     1381 gcctgggtac catgctgatc tgactgctca tcacgcttgc ctttcttttg gtggttcatt
     1441 ctgctacctc attgccatta tttctggaat gtatcagacc acctcttgcc aaggagcaca
     1501 gatctgtaat gaactctcct gtggagtgtt ctgacctaca gaggagagaa attcactgat
     1561 gtgagaggct ctctcagcag catcccatga cttttttttt tttcaaattg aatatcctca
     1621 ggaccttctc attaaatgca cttgtgtgat ggatttttct gtatgcaatt ttctattcta
     1681 gcttgtctac ttttgtgaac ttttgatccc cattttcact gtaggccaca gcagatctag
     1741 aatttctatt gtgcttagaa atgtggagag tgatacctgc agccgacaga ataggaggag
     1801 gtcacagact ccctttcctc ctgcacatgc atctaatgtc ccaatccatg ggtcatctac
     1861 cttagagaca cctatgcatc agggaagtga gaactataga atggctaaga atagcagagg
     1921 catgtaaagg tggaattcac cccagacact gcccacacat ttgggaaaga atgctcagta
     1981 cccagtttct ccctgaggtg agagattgga ctacacatac agcaccctat ttctgggggt
     2041 ctcaatggtc gccggcgatt attactctta caacgcgtgc ggccgcagga acccctagtg
     2101 atggagttgg ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag
     2161 gtcgcccgac gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc tgcaggacat
     2221 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     2281 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     2341 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     2401 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     2461 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     2521 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     2581 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     2641 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     2701 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     2761 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     2821 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     2881 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     2941 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     3001 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     3061 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     3121 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     3181 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     3241 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     3301 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     3361 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     3421 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     3481 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     3541 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     3601 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     3661 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     3721 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     3781 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     3841 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     3901 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     3961 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     4021 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
     4081 cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
     4141 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
     4201 gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca
     4261 gattgtactg agagtgcacc ataaaattgt aaacgttaat attttgttaa aattcgcgtt
     4321 aaatttttgt taaatcagct cattttttaa ccaatagacc gaaatcggca aaatccctta
     4381 taaatcaaaa gaatagcccg agatagagtt gagtgttgtt ccagtttgga acaagagtcc
     4441 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
     4501 cccactacgt gaaccatcac ccaaatcaag ttttttgggg tcgaggtgcc gtaaagcact
     4561 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
     4621 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct aaggcgctgg caagtgtagc
     4681 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgta
     4741 ctatggttgc tttgacgtat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
     4801 cgcatcaggc gcccctgcag gcagctgcgc gctcgctcgc tcactgaggc cgcccgggca
     4861 aagcccgggc gtcgggcgac ctttggtcgc ccggcctcag tgagcgagcg agcgcgcaga
     4921 gagggagtgg ccaactccat cactaggggt tcctgcggcc gctcggtccg cacgtgagat
     4981 ctcatgcctg ctattgtctt cccaatcctc ccccttgctg tcctgcccca ccccaccccc
     5041 cagaatagaa tgacacctac tcagacaatg cgatgcaatt tcctcatttt attaggaaag
     5101 gacagtggga gtggcacctt ccagggtcaa ggaaggcacg ggggaggggc aaacaacaga
     5161 tggctggcaa ctagaaggca cagtcgaaga tctctcgaga agcttgaatt cggcgcgcct
     5221 cagtccccat cttcgagcag cctcaccatg gccccagtct cagagtccag gtttctgatg
     5281 tagttcatca caatgttcac attggtccag ccaccagcct gc
//
