LOCUS       sequence-502371-        5221 bp    DNA     circular SYN 10-MAR-2026
DEFINITION  AiE2197m is an enhancer sequence, designed to drive AAV-mediated 
            transgene expression in specific populations of brain cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5221)
  TITLE     Allen Institute AAV Enhancer Plasmids
REFERENCE   2  (bases 1 to 5221)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 10, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: AiP2010 - pAAV-AiE2197m-minBG-iCre(R297T)-BGHpA
FEATURES             Location/Qualifiers
     source          1..5221
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(449..1240,1241..1309))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1072..1091
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1310..1414)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1482..1500
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1538..1560)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1660..1679
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(1696..2151)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1833..1854)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2045..2064
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     repeat_region   2226..2366
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   2237..2366
                     /label=AAV2 ITR
     polyA_signal    2484..2595
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(3661..3681)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      3684..3693
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     repeat_region   4720..4849
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     rep_origin      complement(join(4911..5221,1..278))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4991..5010)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
       61 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
      121 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
      181 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
      241 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
      301 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
      361 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
      421 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
      481 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
      541 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
      601 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
      661 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
      721 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
      781 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
      841 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
      901 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
      961 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     1021 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     1081 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     1141 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     1201 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     1261 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     1321 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     1381 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     1441 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
     1501 cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
     1561 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
     1621 cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac
     1681 tgagagtgca ccataaaatt gtaaacgtta atattttgtt aaaattcgcg ttaaattttt
     1741 gttaaatcag ctcatttttt aaccaataga ccgaaatcgg caaaatccct tataaatcaa
     1801 aagaatagcc cgagatagag ttgagtgttg ttccagtttg gaacaagagt ccactattaa
     1861 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac
     1921 gtgaaccatc acccaaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga
     1981 accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa
     2041 aggaagggaa gaaagcgaaa ggagcgggcg ctaaggcgct ggcaagtgta gcggtcacgc
     2101 tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg tactatggtt
     2161 gctttgacgt atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
     2221 gcgcccctgc aggcagctgc gcgctcgctc gctcactgag gccgcccggg caaagcccgg
     2281 gcgtcgggcg acctttggtc gcccggcctc agtgagcgag cgagcgcgca gagagggagt
     2341 ggccaactcc atcactaggg gttcctgcgg ccgctcggtc cgcacgtgag atctcatgcc
     2401 tgctattgtc ttcccaatcc tcccccttgc tgtcctgccc caccccaccc cccagaatag
     2461 aatgacacct actcagacaa tgcgatgcaa tttcctcatt ttattaggaa aggacagtgg
     2521 gagtggcacc ttccagggtc aaggaaggca cgggggaggg gcaaacaaca gatggctggc
     2581 aactagaagg cacagtcgaa gatctctcga gaagcttgaa ttcggcgcgc ctcagtcccc
     2641 atcttcgagc agcctcacca tggccccagt ctcagagtcc aggtttctga tgtagttcat
     2701 cacaatgttc acattggtcc agccaccagc ctgcatgatt tcagggatgg acacaccagc
     2761 cctggccatg tcggtggcag cacccactct ggcagagtgg ccagaccagg ccaggtatct
     2821 ctgcccagag tcatccttgg caccatagat caggcggtgg gtggcctcaa agatcccttc
     2881 cagggcccgg gtggacagtt gggaggtggc agaaggggca gccacaccat tctttctgac
     2941 ccggcagaac aggtagttgt tggggtcatc agccacacca gacacagaga tccatctctc
     3001 caccagcttg gtaaccccca gggacagggc cttctccaca ccagctgtgg acaccagggt
     3061 cttggtcctg ccaatgtgga tcagcattct cccaccatcg gtgcgggaga tgtccttcac
     3121 tctgattctg gcaatttcgg caatgcgcag cagggtgttg taggcaatgc ccaggaaggc
     3181 caggttcctg atgtcctggc atctgtcaga gttctccatc agggatctga cttggtcaaa
     3241 gtcagtgcgt tcaaaggcca gggcctgctt ggctctctcc ccagcatcca cattctcctt
     3301 tctgattctc ctcatcacca gggacacagc attggagtca gaagggcgag gcaggccaga
     3361 tctcctgtgc agcatgttga gctggcccag gtgctgttgg atggtcttca cagccaggcc
     3421 tctggcttgc aggtacagga ggtagtccct cacatcctca ggttcagcag ggaaccattt
     3481 cctgttgttc agcttgcacc aggcagccca ggatctgcac acagacagga gcatcttcca
     3541 ggtgtgttca gagaaggcct gcctgtccct gaacatgtcc atcaggttct tcctgacttc
     3601 atcagaggtg gcatccacag ggagggcagg caggttttgg tgcacagtca gcaggttgga
     3661 gactttcctc ttcttcttgg gcaccatggt ggctagcttc gaaagatctg gatcccagaa
     3721 gcaaatgtaa gcaatagatg gctctgccct gacttttatg cccagcccga gctccgccgg
     3781 cggggtgggg atggggtttg ttgactacta agagctggat ggaagccatt ccaggggcaa
     3841 agcacaaact gagctgagac tagggagtgg agagcgagag cagggtcttg gtaaaagtaa
     3901 cccagggcct ctcagatctc tggaggactt gacttgctgg gttgggctct gctggtccaa
     3961 ctggcccaac cacttctctt gggcactacc cattgccact gcgcagagcc ctgccagggg
     4021 atgccaggca aaatctgtca gaagtcccag ctcaacttcc cagtctcaag ggtaaagagg
     4081 tcccgagctc caggctgctc tattctgtgt ggaggagtaa agggcagcca cttccttctg
     4141 aggagaggag tgggcagatc agactgtgga gatctgtgca gttacaaaac acttctgctt
     4201 tcttctgtaa gtggggctgt ttcctttact ttaaaggact caaaacggcc aacagatctt
     4261 tatgtctttt aggtttctag ggctctccct acccatctgc cacctccagc tttgggtcct
     4321 caccagcaag gcaatgccag gacaggtgtc agtgtcttgg cagcctctag gcggttacat
     4381 gacccaggaa tagagctggg tcttttatag tcaggtcaga atcacttgga ggcctggcac
     4441 ctcccactcc ctgctgggaa attcttccct agggcaggca aggctgcaag ctgtccacac
     4501 tagtgcacaa tgacagtggc actaggggac tgtctgcaag agtggttgtg gtggtacagt
     4561 agaggttgga tgtggaaggg tgaaaggtgg gggaggggga agaggtaagg gaatgctgtt
     4621 gccactgagg gcttatgtgg cttgggtccc tggagtacct gtctatagcc ctcggcacct
     4681 cccgccggcg attattactc ttacaacgcg tgcggccgca ggaaccccta gtgatggagt
     4741 tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc
     4801 gacgcccggg cggcctcagt gagcgagcga gcgcgcagct gcctgcagga catgtgagca
     4861 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     4921 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     4981 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     5041 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     5101 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     5161 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     5221 g
//
