LOCUS       sequence-502372-        4849 bp    DNA     circular SYN 10-MAR-2026
DEFINITION  AiE2217m is an enhancer sequence, designed to drive AAV-mediated 
            transgene expression in specific populations of brain cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4849)
  TITLE     Allen Institute AAV Enhancer Plasmids
REFERENCE   2  (bases 1 to 4849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 10, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: AiP2012 - pAAV-AiE2217m-minBG-iCre(R297T)-BGHpA
FEATURES             Location/Qualifiers
     source          1..4849
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(263..1054,1055..1123))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     886..905
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1124..1228)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1296..1314
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1352..1374)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1474..1493
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(1510..1965)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1647..1668)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1859..1878
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     repeat_region   2040..2180
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   2051..2180
                     /label=AAV2 ITR
     polyA_signal    2298..2409
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(3475..3495)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      3498..3507
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     repeat_region   4162..4291
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     rep_origin      complement(join(4353..4849,1..92))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4433..4452)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
       61 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
      121 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
      181 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
      241 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
      301 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
      361 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
      421 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
      481 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
      541 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
      601 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
      661 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
      721 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
      781 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
      841 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
      901 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
      961 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     1021 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     1081 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     1141 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     1201 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
     1261 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
     1321 gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
     1381 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
     1441 gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag
     1501 tgcaccataa aattgtaaac gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa
     1561 tcagctcatt ttttaaccaa tagaccgaaa tcggcaaaat cccttataaa tcaaaagaat
     1621 agcccgagat agagttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg
     1681 tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac
     1741 catcacccaa atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta
     1801 aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag
     1861 ggaagaaagc gaaaggagcg ggcgctaagg cgctggcaag tgtagcggtc acgctgcgcg
     1921 taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtactat ggttgctttg
     1981 acgtatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgccc
     2041 ctgcaggcag ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg
     2101 ggcgaccttt ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa
     2161 ctccatcact aggggttcct gcggccgctc ggtccgcacg tgagatctca tgcctgctat
     2221 tgtcttccca atcctccccc ttgctgtcct gccccacccc accccccaga atagaatgac
     2281 acctactcag acaatgcgat gcaatttcct cattttatta ggaaaggaca gtgggagtgg
     2341 caccttccag ggtcaaggaa ggcacggggg aggggcaaac aacagatggc tggcaactag
     2401 aaggcacagt cgaagatctc tcgagaagct tgaattcggc gcgcctcagt ccccatcttc
     2461 gagcagcctc accatggccc cagtctcaga gtccaggttt ctgatgtagt tcatcacaat
     2521 gttcacattg gtccagccac cagcctgcat gatttcaggg atggacacac cagccctggc
     2581 catgtcggtg gcagcaccca ctctggcaga gtggccagac caggccaggt atctctgccc
     2641 agagtcatcc ttggcaccat agatcaggcg gtgggtggcc tcaaagatcc cttccagggc
     2701 ccgggtggac agttgggagg tggcagaagg ggcagccaca ccattctttc tgacccggca
     2761 gaacaggtag ttgttggggt catcagccac accagacaca gagatccatc tctccaccag
     2821 cttggtaacc cccagggaca gggccttctc cacaccagct gtggacacca gggtcttggt
     2881 cctgccaatg tggatcagca ttctcccacc atcggtgcgg gagatgtcct tcactctgat
     2941 tctggcaatt tcggcaatgc gcagcagggt gttgtaggca atgcccagga aggccaggtt
     3001 cctgatgtcc tggcatctgt cagagttctc catcagggat ctgacttggt caaagtcagt
     3061 gcgttcaaag gccagggcct gcttggctct ctccccagca tccacattct cctttctgat
     3121 tctcctcatc accagggaca cagcattgga gtcagaaggg cgaggcaggc cagatctcct
     3181 gtgcagcatg ttgagctggc ccaggtgctg ttggatggtc ttcacagcca ggcctctggc
     3241 ttgcaggtac aggaggtagt ccctcacatc ctcaggttca gcagggaacc atttcctgtt
     3301 gttcagcttg caccaggcag cccaggatct gcacacagac aggagcatct tccaggtgtg
     3361 ttcagagaag gcctgcctgt ccctgaacat gtccatcagg ttcttcctga cttcatcaga
     3421 ggtggcatcc acagggaggg caggcaggtt ttggtgcaca gtcagcaggt tggagacttt
     3481 cctcttcttc ttgggcacca tggtggctag cttcgaaaga tctggatccc agaagcaaat
     3541 gtaagcaata gatggctctg ccctgacttt tatgcccagc ccgagctccg ccggcgcaat
     3601 gaaggtggag tataagggtt cctcccacta acaaatgctt tgaactatcc aggaataaaa
     3661 ggaatgaaag ggcactccac tctgtggcca gcatgggcca aggctgggag gctgctaaga
     3721 aagtatggct cactgagaaa ctacaaagaa gcacaggcag gggccagttc accaaaggcc
     3781 tctaatgtca tgctaaggat agcggagctg tcccagctgg ctctaaaggt cagctgaaga
     3841 gttcacacag ggaagcactg agcttagacc tgtgtatctg gaagtccact gtggcagtgt
     3901 gcgtaggaag gaatctggag gtggcaggat caaggcagcc ctgggcagga aagtgaagcc
     3961 gatgagtagc aactgggtga atcagagatg gtggtggcaa aaggagcatg gtgacttccc
     4021 cagtgaggag caggagagca ggctcagaaa cctcacctgg tgtaaagtac agggatgaca
     4081 atcctacacc tgcagttagt ggacaagtgg tgaggcttcg tggccgccgg cgattattac
     4141 tcttacaacg cgtgcggccg caggaacccc tagtgatgga gttggccact ccctctctgc
     4201 gcgctcgctc gctcactgag gccgggcgac caaaggtcgc ccgacgcccg ggcggcctca
     4261 gtgagcgagc gagcgcgcag ctgcctgcag gacatgtgag caaaaggcca gcaaaaggcc
     4321 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     4381 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     4441 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     4501 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     4561 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     4621 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     4681 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     4741 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     4801 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttg
//
