LOCUS       sequence-502373-        4935 bp    DNA     circular SYN 10-MAR-2026
DEFINITION  AiE2231m is an enhancer sequence, designed to drive AAV-mediated 
            transgene expression in specific populations of brain cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4935)
  TITLE     Allen Institute AAV Enhancer Plasmids
REFERENCE   2  (bases 1 to 4935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 10, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: AiP2013 - pAAV-AiE2231m-minBG-iCre(R297T)-BGHpA
FEATURES             Location/Qualifiers
     source          1..4935
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      481..490
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             493..513
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1579..1690
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   1808..1948
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   1808..1937
                     /label=AAV2 ITR
     rep_origin      2023..2478
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2110..2129)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2320..2341
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2495..2514)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2614..2636
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2674..2692)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2760..2864
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2865..2933,2934..3725)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3083..3102)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3896..4484
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4385..4404
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   4546..4675
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
ORIGIN
        1 catgtgaaat gtctaatctt ttaaaacaca aatcttcgcc attgatctta atatcctgct
       61 gcagtggggt tccccaggtc aattgtgtgc agtgggggga agccaggttt cctttcatcc
      121 attttaaacg tgcctccttt ttatctcatc ttgtcctctt gttcttcccc aagtaaagcc
      181 atttccattt tctggggacc tagacacaga ggactgagtt gagacaataa tcctcctttg
      241 agcttgtcac ccagaaggcc tcgggcgcgc tgggacatgg agcggtgggt tttagctggt
      301 gacttcaagg tgaggaaaca cttggttcta attagtcaca gttttagttc taaggttctt
      361 tttttaaaaa tgggtgctaa atcctgcaag acgccggcgg agctcgggct gggcataaaa
      421 gtcagggcag agccatctat tgcttacatt tgcttctggg atccagatct ttcgaagcta
      481 gccaccatgg tgcccaagaa gaagaggaaa gtctccaacc tgctgactgt gcaccaaaac
      541 ctgcctgccc tccctgtgga tgccacctct gatgaagtca ggaagaacct gatggacatg
      601 ttcagggaca ggcaggcctt ctctgaacac acctggaaga tgctcctgtc tgtgtgcaga
      661 tcctgggctg cctggtgcaa gctgaacaac aggaaatggt tccctgctga acctgaggat
      721 gtgagggact acctcctgta cctgcaagcc agaggcctgg ctgtgaagac catccaacag
      781 cacctgggcc agctcaacat gctgcacagg agatctggcc tgcctcgccc ttctgactcc
      841 aatgctgtgt ccctggtgat gaggagaatc agaaaggaga atgtggatgc tggggagaga
      901 gccaagcagg ccctggcctt tgaacgcact gactttgacc aagtcagatc cctgatggag
      961 aactctgaca gatgccagga catcaggaac ctggccttcc tgggcattgc ctacaacacc
     1021 ctgctgcgca ttgccgaaat tgccagaatc agagtgaagg acatctcccg caccgatggt
     1081 gggagaatgc tgatccacat tggcaggacc aagaccctgg tgtccacagc tggtgtggag
     1141 aaggccctgt ccctgggggt taccaagctg gtggagagat ggatctctgt gtctggtgtg
     1201 gctgatgacc ccaacaacta cctgttctgc cgggtcagaa agaatggtgt ggctgcccct
     1261 tctgccacct cccaactgtc cacccgggcc ctggaaggga tctttgaggc cacccaccgc
     1321 ctgatctatg gtgccaagga tgactctggg cagagatacc tggcctggtc tggccactct
     1381 gccagagtgg gtgctgccac cgacatggcc agggctggtg tgtccatccc tgaaatcatg
     1441 caggctggtg gctggaccaa tgtgaacatt gtgatgaact acatcagaaa cctggactct
     1501 gagactgggg ccatggtgag gctgctcgaa gatggggact gaggcgcgcc gaattcaagc
     1561 ttctcgagag atcttcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc
     1621 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg
     1681 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg
     1741 acagcaaggg ggaggattgg gaagacaata gcaggcatga gatctcacgt gcggaccgag
     1801 cggccgcagg aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc
     1861 actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg
     1921 agcgagcgag cgcgcagctg cctgcagggg cgcctgatgc ggtattttct ccttacgcat
     1981 ctgtgcggta tttcacaccg catacgtcaa agcaaccata gtacgcgccc tgtagcggcg
     2041 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgcct
     2101 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     2161 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     2221 accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     2281 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     2341 gaacaacact caactctatc tcgggctatt cttttgattt ataagggatt ttgccgattt
     2401 cggtctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     2461 tattaacgtt tacaatttta tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     2521 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     2581 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
     2641 caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg
     2701 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     2761 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     2821 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     2881 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     2941 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     3001 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     3061 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     3121 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     3181 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     3241 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     3301 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     3361 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     3421 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     3481 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     3541 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     3601 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     3661 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     3721 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     3781 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     3841 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     3901 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     3961 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     4021 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     4081 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     4141 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     4201 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     4261 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     4321 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     4381 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     4441 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     4501 cctttttacg gttcctggcc ttttgctggc cttttgctca catgtcctgc aggcagctgc
     4561 gcgctcgctc gctcactgag gccgcccggg cgtcgggcga cctttggtcg cccggcctca
     4621 gtgagcgagc gagcgcgcag agagggagtg gccaactcca tcactagggg ttcctgcggc
     4681 cgcacgcgtt gtaagagtaa taatcgccgg cgtaaatgca tgttgtttta cgacactggc
     4741 ctggagtgct ttgtcgcacc gtggcgtggc ggtaattgga ataccccaaa tctgcctccc
     4801 tgtgaagtct tagatggtcc agagaccttt tttttctttt tacctggccc agttggtgat
     4861 caactgaggc actgaagctc cagaactttt atccctggca ctttcatcct gttgaatatc
     4921 cctgttgatg atatg
//
