LOCUS       sequence-502374-        4792 bp    DNA     circular SYN 10-MAR-2026
DEFINITION  AiE2237m is an enhancer sequence, designed to drive AAV-mediated 
            transgene expression in specific populations of brain cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4792)
  TITLE     Allen Institute AAV Enhancer Plasmids
REFERENCE   2  (bases 1 to 4792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 10, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: AiP2014 - pAAV-AiE2237m-minBG-iCre(R297T)-BGHpA
FEATURES             Location/Qualifiers
     source          1..4792
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      342..351
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             354..374
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1440..1551
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   1669..1809
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   1669..1798
                     /label=AAV2 ITR
     rep_origin      1884..2339
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1971..1990)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2181..2202
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2356..2375)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2475..2497
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2535..2553)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2621..2725
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2726..2794,2795..3586)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2944..2963)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3757..4345
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4246..4265
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   4407..4536
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
ORIGIN
        1 tttctcttcc gtctcagctt ctcctttgct ttagcatcct aggtactggg atcacaggaa
       61 tgccctacat ctctgagcac aaattgtcta aaattggatt cattgtagat attttttgag
      121 acagagtttc actatgtatc cctgactgac ctgtaacttt ttatgtatct accactacag
      181 cttcaaactc acaagagatc ctctgcctct gttttgtgag tgcggggatg cctggctgat
      241 aacttccttt cacgccggcg gagctcgggc tgggcataaa agtcagggca gagccatcta
      301 ttgcttacat ttgcttctgg gatccagatc tttcgaagct agccaccatg gtgcccaaga
      361 agaagaggaa agtctccaac ctgctgactg tgcaccaaaa cctgcctgcc ctccctgtgg
      421 atgccacctc tgatgaagtc aggaagaacc tgatggacat gttcagggac aggcaggcct
      481 tctctgaaca cacctggaag atgctcctgt ctgtgtgcag atcctgggct gcctggtgca
      541 agctgaacaa caggaaatgg ttccctgctg aacctgagga tgtgagggac tacctcctgt
      601 acctgcaagc cagaggcctg gctgtgaaga ccatccaaca gcacctgggc cagctcaaca
      661 tgctgcacag gagatctggc ctgcctcgcc cttctgactc caatgctgtg tccctggtga
      721 tgaggagaat cagaaaggag aatgtggatg ctggggagag agccaagcag gccctggcct
      781 ttgaacgcac tgactttgac caagtcagat ccctgatgga gaactctgac agatgccagg
      841 acatcaggaa cctggccttc ctgggcattg cctacaacac cctgctgcgc attgccgaaa
      901 ttgccagaat cagagtgaag gacatctccc gcaccgatgg tgggagaatg ctgatccaca
      961 ttggcaggac caagaccctg gtgtccacag ctggtgtgga gaaggccctg tccctggggg
     1021 ttaccaagct ggtggagaga tggatctctg tgtctggtgt ggctgatgac cccaacaact
     1081 acctgttctg ccgggtcaga aagaatggtg tggctgcccc ttctgccacc tcccaactgt
     1141 ccacccgggc cctggaaggg atctttgagg ccacccaccg cctgatctat ggtgccaagg
     1201 atgactctgg gcagagatac ctggcctggt ctggccactc tgccagagtg ggtgctgcca
     1261 ccgacatggc cagggctggt gtgtccatcc ctgaaatcat gcaggctggt ggctggacca
     1321 atgtgaacat tgtgatgaac tacatcagaa acctggactc tgagactggg gccatggtga
     1381 ggctgctcga agatggggac tgaggcgcgc cgaattcaag cttctcgaga gatcttcgac
     1441 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
     1501 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
     1561 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     1621 ggaagacaat agcaggcatg agatctcacg tgcggaccga gcggccgcag gaacccctag
     1681 tgatggagtt ggccactccc tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa
     1741 aggtcgcccg acgcccgggc tttgcccggg cggcctcagt gagcgagcga gcgcgcagct
     1801 gcctgcaggg gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     1861 gcatacgtca aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     1921 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ttagcgcccg ctcctttcgc
     1981 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     2041 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgattt
     2101 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     2161 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaactctat
     2221 ctcgggctat tcttttgatt tataagggat tttgccgatt tcggtctatt ggttaaaaaa
     2281 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt
     2341 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc
     2401 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
     2461 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
     2521 cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
     2581 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     2641 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     2701 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     2761 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     2821 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     2881 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     2941 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     3001 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     3061 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     3121 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     3181 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     3241 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     3301 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     3361 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     3421 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     3481 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     3541 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     3601 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     3661 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     3721 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     3781 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     3841 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     3901 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     3961 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     4021 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     4081 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     4141 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     4201 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     4261 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     4321 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     4381 cttttgctgg ccttttgctc acatgtcctg caggcagctg cgcgctcgct cgctcactga
     4441 ggccgcccgg gcgtcgggcg acctttggtc gcccggcctc agtgagcgag cgagcgcgca
     4501 gagagggagt ggccaactcc atcactaggg gttcctgcgg ccgcacgcgt tgtaagagta
     4561 ataatcgccg gcggagtggc cctcctccca gtgagctaga ccctttcaca ttcaatcatc
     4621 aatcaagaca atgtaccaca agtaaattca gtagagtcat ttttttaaaa attaaggttc
     4681 cctcttccca aatgactcta gcctgtgtca agttggcata aactatttta tatatttgga
     4741 tatttatata tatttggact gtgtgaccca ggctggcctc aagctccaaa tc
//
