LOCUS       sequence-502376-        4749 bp    DNA     circular SYN 10-MAR-2026
DEFINITION  AiE2441m is an enhancer sequence, designed to drive AAV-mediated 
            transgene expression in specific populations of brain cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4749)
  TITLE     Allen Institute AAV Enhancer Plasmids
REFERENCE   2  (bases 1 to 4749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 10, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: AiP2108 - pAAV-AiE2441m-minBG-iCre(R297T)-BGHpA
FEATURES             Location/Qualifiers
     source          1..4749
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      321..330
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             333..353
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1419..1530
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   1648..1788
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   1648..1777
                     /label=AAV2 ITR
     rep_origin      1863..2318
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1950..1969)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2160..2181
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2335..2354)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2454..2476
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2514..2532)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2600..2704
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2705..2773,2774..3565)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2923..2942)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3736..4324
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4225..4244
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   4386..4515
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
ORIGIN
        1 agtctgtgtg tctctttgaa attcaaagtt tccagaaata gcatattaat acaaagaagg
       61 aagtcagttt gcttttatca ttagaatccc ccctcccatc tcaggttgtc ccataataac
      121 tggtctttaa agcaatgaga tcaaatttgc agactggctg gaattttcca catcacttac
      181 ctgctcaggc tttagttttt tggtagcctt actacttctt aattgtaagg tttaaaacgg
      241 agctcgggct gggcataaaa gtcagggcag agccatctat tgcttacatt tgcttctggg
      301 atccagatct ttcgaagcta gccaccatgg tgcccaagaa gaagaggaaa gtctccaacc
      361 tgctgactgt gcaccaaaac ctgcctgccc tccctgtgga tgccacctct gatgaagtca
      421 ggaagaacct gatggacatg ttcagggaca ggcaggcctt ctctgaacac acctggaaga
      481 tgctcctgtc tgtgtgcaga tcctgggctg cctggtgcaa gctgaacaac aggaaatggt
      541 tccctgctga acctgaggat gtgagggact acctcctgta cctgcaagcc agaggcctgg
      601 ctgtgaagac catccaacag cacctgggcc agctcaacat gctgcacagg agatctggcc
      661 tgcctcgccc ttctgactcc aatgctgtgt ccctggtgat gaggagaatc agaaaggaga
      721 atgtggatgc tggggagaga gccaagcagg ccctggcctt tgaacgcact gactttgacc
      781 aagtcagatc cctgatggag aactctgaca gatgccagga catcaggaac ctggccttcc
      841 tgggcattgc ctacaacacc ctgctgcgca ttgccgaaat tgccagaatc agagtgaagg
      901 acatctcccg caccgatggt gggagaatgc tgatccacat tggcaggacc aagaccctgg
      961 tgtccacagc tggtgtggag aaggccctgt ccctgggggt taccaagctg gtggagagat
     1021 ggatctctgt gtctggtgtg gctgatgacc ccaacaacta cctgttctgc cgggtcagaa
     1081 agaatggtgt ggctgcccct tctgccacct cccaactgtc cacccgggcc ctggaaggga
     1141 tctttgaggc cacccaccgc ctgatctatg gtgccaagga tgactctggg cagagatacc
     1201 tggcctggtc tggccactct gccagagtgg gtgctgccac cgacatggcc agggctggtg
     1261 tgtccatccc tgaaatcatg caggctggtg gctggaccaa tgtgaacatt gtgatgaact
     1321 acatcagaaa cctggactct gagactgggg ccatggtgag gctgctcgaa gatggggact
     1381 gaggcgcgcc gaattcaagc ttctcgagag atcttcgact gtgccttcta gttgccagcc
     1441 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
     1501 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
     1561 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatga
     1621 gatctcacgt gcggaccgag cggccgcagg aacccctagt gatggagttg gccactccct
     1681 ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct
     1741 ttgcccgggc ggcctcagtg agcgagcgag cgcgcagctg cctgcagggg cgcctgatgc
     1801 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catacgtcaa agcaaccata
     1861 gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     1921 cgctacactt gccagcgcct tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     1981 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     2041 tagtgcttta cggcacctcg accccaaaaa acttgatttg ggtgatggtt cacgtagtgg
     2101 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     2161 tggactcttg ttccaaactg gaacaacact caactctatc tcgggctatt cttttgattt
     2221 ataagggatt ttgccgattt cggtctattg gttaaaaaat gagctgattt aacaaaaatt
     2281 taacgcgaat tttaacaaaa tattaacgtt tacaatttta tggtgcactc tcagtacaat
     2341 ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc
     2401 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
     2461 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt
     2521 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     2581 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     2641 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     2701 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     2761 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     2821 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     2881 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     2941 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     3001 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     3061 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     3121 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     3181 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     3241 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     3301 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     3361 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     3421 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     3481 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     3541 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     3601 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     3661 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     3721 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     3781 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     3841 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     3901 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     3961 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     4021 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     4081 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     4141 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     4201 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     4261 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     4321 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     4381 catgtcctgc aggcagctgc gcgctcgctc gctcactgag gccgcccggg cgtcgggcga
     4441 cctttggtcg cccggcctca gtgagcgagc gagcgcgcag agagggagtg gccaactcca
     4501 tcactagggg ttcctgcggc cgcacgcgtt gtaagagtaa taatcgttaa aataaggaat
     4561 atgaaatcaa agactaatga atagaggtat tcaaaacacc atgagtctag agagggttgt
     4621 cttagggtac ccctggcttt cccagagttc acgcctagac taatttactt tggtatttca
     4681 aacctcacat tcttttcctc acctcagact tggagctctc aagactttga attcttcttt
     4741 cataatttt
//
