LOCUS       sequence-502377-        4800 bp    DNA     circular SYN 10-MAR-2026
DEFINITION  AiE3331m is an enhancer sequence, designed to drive AAV-mediated 
            transgene expression in specific populations of brain cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4800)
  TITLE     Allen Institute AAV Enhancer Plasmids
REFERENCE   2  (bases 1 to 4800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 10, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: AiP20498 - pAAV-AiE3331m-minBG-iCre(R297T)-BGHpA
FEATURES             Location/Qualifiers
     source          1..4800
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   262..391
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     rep_origin      complement(453..1041)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(533..552)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1212..2003,2004..2072))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1835..1854
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2073..2177)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2245..2263
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2301..2323)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2423..2442
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(2459..2914)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2596..2617)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2808..2827
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     repeat_region   2989..3129
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3000..3129
                     /label=AAV2 ITR
     polyA_signal    3247..3358
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(4424..4444)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      4447..4456
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cagttacata atgatgatgt cacttgactt tcagattata aaaagaagaa gaacatttta
       61 ctataaatag ttttcctgtg atggctgaaa ttcactgtca acatgtttgg ctttgacaaa
      121 atcaaaagcg cttcatgata aaagtcctgg agaactaaga ttcaaaggac ataactcaac
      181 atgataacag cattttgcat taagcagtta gacattacca acataaacag agagaaattc
      241 caagcatacg cgtgcggccg caggaacccc tagtgatgga gttggccact ccctctctgc
      301 gcgctcgctc gctcactgag gccgggcgac caaaggtcgc ccgacgcccg ggcggcctca
      361 gtgagcgagc gagcgcgcag ctgcctgcag gacatgtgag caaaaggcca gcaaaaggcc
      421 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
      481 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
      541 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
      601 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
      661 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
      721 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
      781 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
      841 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
      901 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
      961 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     1021 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     1081 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     1141 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     1201 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     1261 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     1321 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     1381 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     1441 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     1501 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     1561 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     1621 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     1681 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     1741 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     1801 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     1861 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     1921 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     1981 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     2041 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     2101 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     2161 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
     2221 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt
     2281 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
     2341 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
     2401 tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccataaa
     2461 attgtaaacg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt
     2521 tttaaccaat agaccgaaat cggcaaaatc ccttataaat caaaagaata gcccgagata
     2581 gagttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac
     2641 gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcacccaaa
     2701 tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc
     2761 cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg
     2821 aaaggagcgg gcgctaaggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca
     2881 cccgccgcgc ttaatgcgcc gctacagggc gcgtactatg gttgctttga cgtatgcggt
     2941 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgcccc tgcaggcagc
     3001 tgcgcgctcg ctcgctcact gaggccgccc gggcaaagcc cgggcgtcgg gcgacctttg
     3061 gtcgcccggc ctcagtgagc gagcgagcgc gcagagaggg agtggccaac tccatcacta
     3121 ggggttcctg cggccgctcg gtccgcacgt gagatctcat gcctgctatt gtcttcccaa
     3181 tcctccccct tgctgtcctg ccccacccca ccccccagaa tagaatgaca cctactcaga
     3241 caatgcgatg caatttcctc attttattag gaaaggacag tgggagtggc accttccagg
     3301 gtcaaggaag gcacggggga ggggcaaaca acagatggct ggcaactaga aggcacagtc
     3361 gaagatctct cgagaagctt gaattcggcg cgcctcagtc cccatcttcg agcagcctca
     3421 ccatggcccc agtctcagag tccaggtttc tgatgtagtt catcacaatg ttcacattgg
     3481 tccagccacc agcctgcatg atttcaggga tggacacacc agccctggcc atgtcggtgg
     3541 cagcacccac tctggcagag tggccagacc aggccaggta tctctgccca gagtcatcct
     3601 tggcaccata gatcaggcgg tgggtggcct caaagatccc ttccagggcc cgggtggaca
     3661 gttgggaggt ggcagaaggg gcagccacac cattctttct gacccggcag aacaggtagt
     3721 tgttggggtc atcagccaca ccagacacag agatccatct ctccaccagc ttggtaaccc
     3781 ccagggacag ggccttctcc acaccagctg tggacaccag ggtcttggtc ctgccaatgt
     3841 ggatcagcat tctcccacca tcggtgcggg agatgtcctt cactctgatt ctggcaattt
     3901 cggcaatgcg cagcagggtg ttgtaggcaa tgcccaggaa ggccaggttc ctgatgtcct
     3961 ggcatctgtc agagttctcc atcagggatc tgacttggtc aaagtcagtg cgttcaaagg
     4021 ccagggcctg cttggctctc tccccagcat ccacattctc ctttctgatt ctcctcatca
     4081 ccagggacac agcattggag tcagaagggc gaggcaggcc agatctcctg tgcagcatgt
     4141 tgagctggcc caggtgctgt tggatggtct tcacagccag gcctctggct tgcaggtaca
     4201 ggaggtagtc cctcacatcc tcaggttcag cagggaacca tttcctgttg ttcagcttgc
     4261 accaggcagc ccaggatctg cacacagaca ggagcatctt ccaggtgtgt tcagagaagg
     4321 cctgcctgtc cctgaacatg tccatcaggt tcttcctgac ttcatcagag gtggcatcca
     4381 cagggagggc aggcaggttt tggtgcacag tcagcaggtt ggagactttc ctcttcttct
     4441 tgggcaccat ggtggctagc ttcgaaagat ctggatccca gaagcaaatg taagcaatag
     4501 atggctctgc cctgactttt atgcccagcc cgagctccgc cggcgatttc agagcaatac
     4561 ttagtcattc aagaaaacaa aacaaatatt aatagtatta ctggatgtta cagaaaatgt
     4621 gtgccaaaaa gtcttatgaa aatgcactca acctcatgta gttaaagaaa tcgaaatgaa
     4681 aaatacataa gcaatggtgg taatactaaa aatcaaccat ctatgagatt tggggcaaaa
     4741 agtttatcaa agtacaacag ataacacaga tggtgtatca tagaaacaac agtaaatgtt
//
