LOCUS       sequence-50238-e         684 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 684)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 684)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: RRAD
FEATURES             Location/Qualifiers
     source          1..684
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             35..52
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             77..97
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 aattttgttt actttagaag gagatataca tatgcaccat catcatcatc attcttctgg
       61 tgtagatctg ggtaccgaga acctgtactt ccaatccatg tcagacgaga gcgtttacaa
      121 ggtgctgctg ctgggggcgc ccggcgtggg caagagcgcc ctggcgcgca tcttcggcgg
      181 tgtggaggac gggcctgaag cagaggcagc agggcacacc tatgatcgct ccattgtagt
      241 ggacggagaa gaggcatcac tcatggtcta cgacatttgg gagcaggacg ggggccgctg
      301 gttgcccggc cactgcatgg ccatggggga tgcctatgtc attgtgtact cagtgacgga
      361 caagggcagc ttcgagaagg cctcagaact gcgggtccag ctgcggcgtg cacggcaaac
      421 agatgatgtg cccatcatcc tcgtgggcaa caagagcgac ctggtgcgct ctcgtgaggt
      481 ctcggtggat gagggccggg cctgcgcggt ggtctttgac tgcaagttca ttgagacatc
      541 agcggcattg caccacaatg tccaggcgct gtttgaaggt gtcgtgcgcc agatacgcct
      601 gcgcagggac agcaaagaag cctgacagta aaggtggata cggatccgaa ttcgagctcc
      661 gtcgacaagc ttgcggccgc actc
//
