LOCUS       sequence-502425-        9126 bp    DNA     circular SYN 10-MAR-2026
DEFINITION  FAP tagged STE3 (N-terminally tagged, optimized for yeast 
            expression) under the Tef1 promoter with 2xMYC tag and MFA1 signal 
            sequence to help target construct to the ER .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9126)
  AUTHORS   Oppenheimer KG, Hager NA, McAtee CK, Filiztekin E, Shang C, Warnick 
            JA, Bruchez MP, Brodsky JL, Prosser DC, Kwiatkowski AV, O'Donnell AF
  TITLE     Optimization of the fluorogen-activating protein tag for 
            quantitative protein trafficking and colocalization studies in S. 
            cerevisiae.
  JOURNAL   Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi: 10.1091/mbc.E24-04-0174. 
            Epub 2024 May 29.
  PUBMED    38809589
REFERENCE   2  (bases 1 to 9126)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 10, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell."; date: "2024-07-1"; volume: "35(7):mr5. doi"; pages: " 
            10.1091/mbc.E24-04-0174. Epub 2024 May 29"
COMMENT     Sequence Label: pRS415-TEF1pr-MFA-Igkappa-FAPoptim-STE3
FEATURES             Location/Qualifiers
     source          1..9126
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..399
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     sig_peptide     658..717
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     CDS             1456..1485
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1486..1515
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      2962..3209
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(2978..2996)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(3227..3246)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3228..3246)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3256..3273)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3256..3272)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3265..3287)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3413..3868
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3500..3519)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3710..3731
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4169..4576
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             4577..5671
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(6165..6184)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6284..6306
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6344..6362)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    6404..6907
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        6944..7048
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7049..7117,7118..7909)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7267..7286)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8080..8668
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8569..8588
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8822..8839
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8956..8977
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8992..8997,8998..9015,9016..9022)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9030..9046
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9035..9057
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9054..9070
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9054..9070
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9089..9109
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9091..9109
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 catagcttca aaatgtttct actccttttt tactcttcca gattttctcg gactccgcgc
       61 atcgccgtac cacttcaaaa cacccaagca cagcatacta aatttcccct ctttcttcct
      121 ctagggtgtc gttaattacc cgtactaaag gtttggaaaa gaaaaaagag accgcctcgt
      181 ttctttttct tcgtcgaaaa aggcaataaa aatttttatc acgtttcttt ttcttgaaaa
      241 tttttttttt gatttttttc tctttcgatg acctcccatt gatatttaag ttaataaacg
      301 gtcttcaatt tctcaagttt cagtttcatt tttcttgttc tattacaact ttttttactt
      361 cttgctcatt agaaagaaag catagcaatc taatctaagt tttctagaat gagatttcct
      421 tcaattttta ctgcagtttt attcgcagca tcctccgcat tagctgctcc agtcaacact
      481 acaacagaag atgaaacggc acaaattccg gctgaagctg tcatcggtta cttagattta
      541 gaaggggatt tcgatgttgc tgttttgcca ttttccaaca gcacaaataa cgggttattg
      601 tttataaata ctactattgc cagcattgct gctaaagaag aaggggtatc tttggatatg
      661 gagacagaca cactcctgct atgggtactg ctgctctggg ttccaggttc cactggtgac
      721 actagtggat ccatgcaagc tgttgttact caagaaccat ctgttactgt ttctccaggt
      781 ggtactgtta tcttgacttg tggttctggt actggtgctg ttacttctgg tcactacgct
      841 aactggttcc aacaaaagcc aggtcaagct ccaagagctt tgatcttcga cactgacaag
      901 aagtactctt ggactccagg tagattctct ggttctttgt tgggtgctaa ggctgctttg
      961 actatctctg acgctcaacc agaagacgaa gctgaatact actgttcttt gtctgacgtt
     1021 gacggttact tgttcggtgg tggtactcaa ttgactgttt tgtctggtgg tggtggttct
     1081 ggtggtggtg gttctggtgg tggtggttct ggtggtggtg gttctcaagc tgttgttact
     1141 caagaaccat ctgttactgt ttctccaggt ggtactgtta tcttgacttg tggttctggt
     1201 actggtgctg ttacttctgg tcactacgct aactggttcc aacaaaagcc aggtcaagct
     1261 ccaagagctt tgatcttcga cactgacaag aagtactctt ggactccagg tagattctct
     1321 ggttctttgt tgggtgctaa ggctgctttg actatctctg acgctcaacc agaagacgaa
     1381 gctgaatact actgttcttt gtctgacgtt gacggttact tgttcggtgg tggtactcaa
     1441 ttgactgttt tgtctgagca gaagctgatc agcgaggagg acctggagca gaagctgatc
     1501 agcgaggagg acctggcggg tgcgggtgcg ggtcgccccg ggatgtcata caagtcagca
     1561 ataatagggc tttgtttgct agctgtgata ctattagctc cccctttagc atggcattca
     1621 cataccaaga atattccagc aatcattttg ataacatggc ttcttacaat gaatttaacg
     1681 tgtattgtag atgcggcaat atggagtgac gacgatttcc tcacgagatg ggatggtaaa
     1741 ggttggtgtg atattgtcat caagttgcag gttggtgcga atattggcat atcatgtgcc
     1801 gttaccaaca tcatttacaa cttgcataca attttgaagg cagatagtgt tttaccggat
     1861 ctttcatcat ggacgaaaat cgtcaaggac cttgtgatta gcttgttcac acctgtcatg
     1921 gtcatgggat tttcatatct gttacaagta tttagatatg gtatcgctcg ttacaacggt
     1981 tgccaaaact tattgtctcc gacatggatt accactgttt tgtataccat gtggatgctt
     2041 atatggtcat ttgtgggcgc tgtttatgcc accttagtac tgttcgtgtt ttataaaaaa
     2101 cgcaaggacg ttagggatat tttacactgt accaattcag gtttaaacct gacaaggttc
     2161 gcaaggctgt tgatattctg tttcattatt attttagtca tgttcccttt ttctgtttac
     2221 acctttgttc aagatttaca gcaggtagaa ggacactata cttttaaaaa tacccattcc
     2281 agcaccatct ggaataccat tattaaattt gaccctggca gaccaattta taatatatgg
     2341 ctttatgttt tgatgtctta cctagtattt ctaatctttg gcttaggttc tgatgctttg
     2401 catatgtact ctaaattcct gcgttccatc aaactaggat ttgtacttga catgtggaaa
     2461 agattcattg ataagaataa ggaaaaacga gtaggcatat tgctaaacaa gctgtcctca
     2521 cgcaaagaga gccgtaaccc attttctaca gactctgaga actatatctc cacgtgtaca
     2581 gaaaactatt ctccctgtgt aggtacacca atatcacaag cgcatttcta tgtcgactat
     2641 aggattccag atgatcctag aaaatctcaa aataaaagca aaaaatattt gtttgctgat
     2701 aaagaaacag atgatattct tgatgaaata gacctaaaag aaagtaggca catcccttac
     2761 gtcacgcaag gacagagctt tgacgacgaa atatcacttg gaggattctc aaaagttact
     2821 ctcgattatt cagaaaagct tcataattct gcaagctcca attttgaagg ggaaagtctt
     2881 tgctactctc cagcttcaaa agaagagaat tcaagctcaa acgaacatag ttcagaaaat
     2941 actgcaggcc cttaactcga gtcatgtaat tagttatgtc acgcttacat tcacgccctc
     3001 cccccacatc cgctctaacc gaaaaggaag gagttagaca acctgaagtc taggtcccta
     3061 tttatttttt tatagttatg ttagtattaa gaacgttatt tatatttcaa atttttcttt
     3121 tttttctgta cagacgcgtg tacgcatgta acattatact gaaaaccttg cttgagaagg
     3181 ttttgggacg ctcgaaggct ttaatttgcg gccggtaccc aattcgccct atagtgagtc
     3241 gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     3301 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     3361 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggacgcgccc
     3421 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     3481 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     3541 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     3601 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     3661 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     3721 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     3781 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     3841 tttaacaaaa tattaacgct tacaatttcc tgatgcggta ttttctcctt acgcatctgt
     3901 gcggtatttc acaccgcata tcgaccggtc gaggagaact tctagtatat ctacatacct
     3961 aatattattg ccttattaaa aatggaatcc caacaattac atcaaaatcc acattctctt
     4021 caaaatcaat tgtcctgtac ttccttgttc atgtgtgttc aaaaacgtta tatttatagg
     4081 ataattatac tctatttctc aacaagtaat tggttgtttg gccgagcggt ctaaggcgcc
     4141 tgattcaaga aatatcttga ccgcagttaa ctgtgggaat actcaggtat cgtaagatgc
     4201 aagagttcga atctcttagc aaccattatt tttttcctca acataacgag aacacacagg
     4261 ggcgctatcg cacagaatca aattcgatga ctggaaattt tttgttaatt tcagaggtcg
     4321 cctgacgcat ataccttttt caactgaaaa attgggagaa aaaggaaagg tgagagcgcc
     4381 ggaaccggct tttcatatag aatagagaag cgttcatgac taaatgcttg catcacaata
     4441 cttgaagttg acaatattat ttaaggacct attgtttttt ccaataggtg gttagcaatc
     4501 gtcttacttt ctaacttttc ttacctttta catttcagca atatatatat atatatttca
     4561 aggatatacc attctaatgt ctgcccctaa gaagatcgtc gttttgccag gtgaccacgt
     4621 tggtcaagaa atcacagccg aagccattaa ggttcttaaa gctatttctg atgttcgttc
     4681 caatgtcaag ttcgatttcg aaaatcattt aattggtggt gctgctatcg atgctacagg
     4741 tgttccactt ccagatgagg cgctggaagc ctccaagaag gctgatgccg ttttgttagg
     4801 tgctgtgggt ggtcctaaat ggggtaccgg tagtgttaga cctgaacaag gtttactaaa
     4861 aatccgtaaa gaacttcaat tgtacgccaa cttaagacca tgtaactttg catccgactc
     4921 tcttttagac ttatctccaa tcaagccaca atttgctaaa ggtactgact tcgttgttgt
     4981 cagagaatta gtgggaggta tttactttgg taagagaaag gaagacgatg gtgatggtgt
     5041 cgcttgggat agtgaacaat acaccgttcc agaagtgcaa agaatcacaa gaatggccgc
     5101 tttcatggcc ctacaacatg agccaccatt gcctatttgg tccttggata aagctaatgt
     5161 tttggcctct tcaagattat ggagaaaaac tgtggaggaa accatcaaga acgaattccc
     5221 tacattgaag gttcaacatc aattgattga ttctgccgcc atgatcctag ttaagaaccc
     5281 aacccaccta aatggtatta taatcaccag caacatgttt ggtgatatca tctccgatga
     5341 agcctccgtt atcccaggtt ccttgggttt gttgccatct gcgtccttgg cctctttgcc
     5401 agacaagaac accgcatttg gtttgtacga accatgccac ggttctgctc cagatttgcc
     5461 aaagaataag gtcaacccta tcgccactat cttgtctgct gcaatgatgt tgaaattgtc
     5521 attgaacttg cctgaagaag gtaaggccat tgaagatgca gttaaaaagg ttttggatgc
     5581 aggtatcaga actggtgatt taggtggttc caacagtacc accgaagtcg gtgatgctgt
     5641 cgccgaagaa gttaagaaaa tccttgctta aaaagattct ctttttttat gatatttgta
     5701 cataaacttt ataaatgaaa ttcataatag aaacgacacg aaattacaaa atggaatatg
     5761 ttcatagggt agacgaaact atatacgcaa tctacataca tttatcaaga aggagaaaaa
     5821 ggaggatgta aaggaataca ggtaagcaaa ttgatactaa tggctcaacg tgataaggaa
     5881 aaagaattgc actttaacat taatattgac aaggaggagg gcaccacaca aaaagttagg
     5941 tgtaacagaa aatcatgaaa ctatgattcc taatttatat attggaggat tttctctaaa
     6001 aaaaaaaaaa tacaacaaat aaaaaacact caatgacctg accatttgat ggagtttaag
     6061 tcaatacctt cttgaaccat ttcccataat ggtgaaagtt ccctcaagaa ttttactctg
     6121 tcagaaacgg ccttaacgac gtagtcgata tggtgcactc tcagtacaat ctgctctgat
     6181 gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
     6241 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
     6301 cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta
     6361 tttttatagg ttaatgtcat gataataatg gtttcttagg acggatcgct tgcctgtaac
     6421 ttacacgcgc ctcgtatctt ttaatgatgg aataatttgg gaatttactc tgtgtttatt
     6481 tatttttatg ttttgtattt ggattttaga aagtaaataa agaaggtaga agagttacgg
     6541 aatgaagaaa aaaaaataaa caaaggttta aaaaatttca acaaaaagcg tactttacat
     6601 atatatttat tagacaagaa aagcagatta aatagatata cattcgatta acgataagta
     6661 aaatgtaaaa tcacaggatt ttcgtgtgtg gtcttctaca cagacaagat gaaacaattc
     6721 ggcattaata cctgagagca ggaagagcaa gataaaaggt agtatttgtt ggcgatcccc
     6781 ctagagtctt ttacatcttc ggaaaacaaa aactattttt tctttaattt ctttttttac
     6841 tttctatttt taatttatat atttatatta aaaaatttaa attataatta tttttatagc
     6901 acgtgatgaa aaggacccag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     6961 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     7021 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     7081 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     7141 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     7201 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     7261 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     7321 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     7381 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     7441 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     7501 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     7561 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     7621 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     7681 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     7741 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     7801 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     7861 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     7921 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     7981 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     8041 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     8101 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     8161 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     8221 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     8281 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     8341 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     8401 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     8461 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     8521 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     8581 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     8641 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     8701 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     8761 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     8821 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
     8881 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
     8941 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
     9001 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
     9061 cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct
     9121 ggagct
//
