LOCUS       sequence-502433-        9224 bp    DNA     circular SYN 10-MAR-2026
DEFINITION  FAP tagged STE3-PEST mutant (delta413-470 - N-terminally tagged, 
            optimized) under the Ste3 promoter with 2xMYC tag and MFA1 signal 
            sequence to help target construct to the ER .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9224)
  AUTHORS   Oppenheimer KG, Hager NA, McAtee CK, Filiztekin E, Shang C, Warnick 
            JA, Bruchez MP, Brodsky JL, Prosser DC, Kwiatkowski AV, O'Donnell AF
  TITLE     Optimization of the fluorogen-activating protein tag for 
            quantitative protein trafficking and colocalization studies in S. 
            cerevisiae.
  JOURNAL   Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi: 10.1091/mbc.E24-04-0174. 
            Epub 2024 May 29.
  PUBMED    38809589
REFERENCE   2  (bases 1 to 9224)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 10, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell."; date: "2024-07-1"; volume: "35(7):mr5. doi"; pages: " 
            10.1091/mbc.E24-04-0174. Epub 2024 May 29"
COMMENT     Sequence Label: pRS415-STE3pr-MFA-Igkappa-FAPoptim-STE3-deltaPEST
FEATURES             Location/Qualifiers
     source          1..9224
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..22
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(37..42,43..60,61..67)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    75..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     80..102
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     99..115
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     99..115
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     134..154
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        136..154
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     sig_peptide     928..987
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     CDS             1726..1755
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1756..1785
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      3231..3478
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(3247..3265)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(3496..3515)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3497..3515)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3525..3542)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3525..3541)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3534..3556)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3682..4137
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3769..3788)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3979..4000
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4438..4845
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             4846..5940
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(6434..6453)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6553..6575
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6613..6631)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    6673..7176
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        7213..7317
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7318..7386,7387..8178)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7536..7555)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8349..8937
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8838..8857
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9091..9108
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
       61 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      121 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tcatttacac
      181 caccagaagc gttctggctc cccttttctc taaacgttaa acattttaca attgaaatgt
      241 taccaatcct atattattgt accacattgc cagatttatg aactctgggt atgggtgcta
      301 attttcgtta gaagcgctgg tacaattttc tctgtcattg tgacactaat taggaaactt
      361 ctcgactatc aatgtgtaaa tgaaggaata atggcggaaa ctttgaaact ttgtcaataa
      421 ttgcatcatt ggatgcgttt catttggccg ttatcacgga gaggcagagt tctctccaca
      481 atttgggcag aagtcttttg aaaagacata tatatatata tatatgtata tgagtggatg
      541 cttaaggtaa gaataatttc tgaattccca agtattcatt ttgtgcagta ttcacatatt
      601 ctattttatt gctttttaac tttagaggca attaaatttg tgtaggaaag gcaaaatact
      661 atcaaaattt tctctagaat gagatttcct tcaattttta ctgcagtttt attcgcagca
      721 tcctccgcat tagctgctcc agtcaacact acaacagaag atgaaacggc acaaattccg
      781 gctgaagctg tcatcggtta cttagattta gaaggggatt tcgatgttgc tgttttgcca
      841 ttttccaaca gcacaaataa cgggttattg tttataaata ctactattgc cagcattgct
      901 gctaaagaag aaggggtatc tttggatatg gagacagaca cactcctgct atgggtactg
      961 ctgctctggg ttccaggttc cactggtgac actagtggat ccatgcaagc tgttgttact
     1021 caagaaccat ctgttactgt ttctccaggt ggtactgtta tcttgacttg tggttctggt
     1081 actggtgctg ttacttctgg tcactacgct aactggttcc aacaaaagcc aggtcaagct
     1141 ccaagagctt tgatcttcga cactgacaag aagtactctt ggactccagg tagattctct
     1201 ggttctttgt tgggtgctaa ggctgctttg actatctctg acgctcaacc agaagacgaa
     1261 gctgaatact actgttcttt gtctgacgtt gacggttact tgttcggtgg tggtactcaa
     1321 ttgactgttt tgtctggtgg tggtggttct ggtggtggtg gttctggtgg tggtggttct
     1381 ggtggtggtg gttctcaagc tgttgttact caagaaccat ctgttactgt ttctccaggt
     1441 ggtactgtta tcttgacttg tggttctggt actggtgctg ttacttctgg tcactacgct
     1501 aactggttcc aacaaaagcc aggtcaagct ccaagagctt tgatcttcga cactgacaag
     1561 aagtactctt ggactccagg tagattctct ggttctttgt tgggtgctaa ggctgctttg
     1621 actatctctg acgctcaacc agaagacgaa gctgaatact actgttcttt gtctgacgtt
     1681 gacggttact tgttcggtgg tggtactcaa ttgactgttt tgtctgagca gaagctgatc
     1741 agcgaggagg acctggagca gaagctgatc agcgaggagg acctggcggg tgcgggtgcg
     1801 ggtcgccccg ggatgtcata caagtcagca ataatagggc tttgtttgct agctgtgata
     1861 ctattagctc cccctttagc atggcattca cataccaaga atattccagc aatcattttg
     1921 ataacatggc ttcttacaat gaatttaacg tgtattgtag atgcggcaat atggagtgac
     1981 gacgatttcc tcacgagatg ggatggtaaa ggttggtgtg atattgtcat caagttgcag
     2041 gttggtgcga atattggcat atcatgtgcc gttaccaaca tcatttacaa cttgcataca
     2101 attttgaagg cagatagtgt tttaccggat ctttcatcat ggacgaaaat cgtcaaggac
     2161 cttgtgatta gcttgttcac acctgtcatg gtcatgggat tttcatatct gttacaagta
     2221 tttagatatg gtatcgctcg ttacaacggt tgccaaaact tattgtctcc gacatggatt
     2281 accactgttt tgtataccat gtggatgctt atatggtcat ttgtgggcgc tgtttatgcc
     2341 accttagtac tgttcgtgtt ttataaaaaa cgcaaggacg ttagggatat tttacactgt
     2401 accaattcag gtttaaacct gacaaggttc gcaaggctgt tgatattctg tttcattatt
     2461 attttagtca tgttcccttt ttctgtttac acctttgttc aagatttaca gcaggtagaa
     2521 ggacactata cttttaaaaa tacccattcc agcaccatct ggaataccat tattaaattt
     2581 gaccctggca gaccaattta taatatatgg ctttatgttt tgatgtctta cctagtattt
     2641 ctaatctttg gcttaggttc tgatgctttg catatgtact ctaaattcct gcgttccatc
     2701 aaactaggat ttgtacttga catgtggaaa agattcattg ataagaataa ggaaaaacga
     2761 gtaggcatat tgctaaacaa gctgtcctca cgcaaagaga gccgtaaccc attttctaca
     2821 gactctgaga actatatctc cacgtgtaca gaaaactatt ctccctgtgt aggtacacca
     2881 atatcacaag cgcatttcta tgtcgactat aggattccag atgatcctag aaaatctcaa
     2941 aataaaagca aaaaatattt gtttgctgat aaagaaacag atgatattct tgatgaaata
     3001 gacctaaaag aaagtaggca catcccttac gtcacgcaag gacagagcta agacgacgaa
     3061 atatcacttg gaggattctc aaaagttact ctcgattatt cagaaaagct tcataattct
     3121 gcaagctcca attttgaagg ggaaagtctt tgctactctc cagcttcaaa agaagagaat
     3181 tcaagctcaa acgaacatag ttcagaaaat actgcaggcc cttactcgag tcatgtaatt
     3241 agttatgtca cgcttacatt cacgccctcc ccccacatcc gctctaaccg aaaaggaagg
     3301 agttagacaa cctgaagtct aggtccctat ttattttttt atagttatgt tagtattaag
     3361 aacgttattt atatttcaaa tttttctttt ttttctgtac agacgcgtgt acgcatgtaa
     3421 cattatactg aaaaccttgc ttgagaaggt tttgggacgc tcgaaggctt taatttgcgg
     3481 ccggtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta
     3541 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     3601 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     3661 cgcagcctga atggcgaatg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     3721 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     3781 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     3841 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     3901 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     3961 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     4021 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     4081 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttcct
     4141 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat cgaccggtcg
     4201 aggagaactt ctagtatatc tacataccta atattattgc cttattaaaa atggaatccc
     4261 aacaattaca tcaaaatcca cattctcttc aaaatcaatt gtcctgtact tccttgttca
     4321 tgtgtgttca aaaacgttat atttatagga taattatact ctatttctca acaagtaatt
     4381 ggttgtttgg ccgagcggtc taaggcgcct gattcaagaa atatcttgac cgcagttaac
     4441 tgtgggaata ctcaggtatc gtaagatgca agagttcgaa tctcttagca accattattt
     4501 ttttcctcaa cataacgaga acacacaggg gcgctatcgc acagaatcaa attcgatgac
     4561 tggaaatttt ttgttaattt cagaggtcgc ctgacgcata tacctttttc aactgaaaaa
     4621 ttgggagaaa aaggaaaggt gagagcgccg gaaccggctt ttcatataga atagagaagc
     4681 gttcatgact aaatgcttgc atcacaatac ttgaagttga caatattatt taaggaccta
     4741 ttgttttttc caataggtgg ttagcaatcg tcttactttc taacttttct taccttttac
     4801 atttcagcaa tatatatata tatatttcaa ggatatacca ttctaatgtc tgcccctaag
     4861 aagatcgtcg ttttgccagg tgaccacgtt ggtcaagaaa tcacagccga agccattaag
     4921 gttcttaaag ctatttctga tgttcgttcc aatgtcaagt tcgatttcga aaatcattta
     4981 attggtggtg ctgctatcga tgctacaggt gttccacttc cagatgaggc gctggaagcc
     5041 tccaagaagg ctgatgccgt tttgttaggt gctgtgggtg gtcctaaatg gggtaccggt
     5101 agtgttagac ctgaacaagg tttactaaaa atccgtaaag aacttcaatt gtacgccaac
     5161 ttaagaccat gtaactttgc atccgactct cttttagact tatctccaat caagccacaa
     5221 tttgctaaag gtactgactt cgttgttgtc agagaattag tgggaggtat ttactttggt
     5281 aagagaaagg aagacgatgg tgatggtgtc gcttgggata gtgaacaata caccgttcca
     5341 gaagtgcaaa gaatcacaag aatggccgct ttcatggccc tacaacatga gccaccattg
     5401 cctatttggt ccttggataa agctaatgtt ttggcctctt caagattatg gagaaaaact
     5461 gtggaggaaa ccatcaagaa cgaattccct acattgaagg ttcaacatca attgattgat
     5521 tctgccgcca tgatcctagt taagaaccca acccacctaa atggtattat aatcaccagc
     5581 aacatgtttg gtgatatcat ctccgatgaa gcctccgtta tcccaggttc cttgggtttg
     5641 ttgccatctg cgtccttggc ctctttgcca gacaagaaca ccgcatttgg tttgtacgaa
     5701 ccatgccacg gttctgctcc agatttgcca aagaataagg tcaaccctat cgccactatc
     5761 ttgtctgctg caatgatgtt gaaattgtca ttgaacttgc ctgaagaagg taaggccatt
     5821 gaagatgcag ttaaaaaggt tttggatgca ggtatcagaa ctggtgattt aggtggttcc
     5881 aacagtacca ccgaagtcgg tgatgctgtc gccgaagaag ttaagaaaat ccttgcttaa
     5941 aaagattctc tttttttatg atatttgtac ataaacttta taaatgaaat tcataataga
     6001 aacgacacga aattacaaaa tggaatatgt tcatagggta gacgaaacta tatacgcaat
     6061 ctacatacat ttatcaagaa ggagaaaaag gaggatgtaa aggaatacag gtaagcaaat
     6121 tgatactaat ggctcaacgt gataaggaaa aagaattgca ctttaacatt aatattgaca
     6181 aggaggaggg caccacacaa aaagttaggt gtaacagaaa atcatgaaac tatgattcct
     6241 aatttatata ttggaggatt ttctctaaaa aaaaaaaaat acaacaaata aaaaacactc
     6301 aatgacctga ccatttgatg gagtttaagt caataccttc ttgaaccatt tcccataatg
     6361 gtgaaagttc cctcaagaat tttactctgt cagaaacggc cttaacgacg tagtcgatat
     6421 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc
     6481 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
     6541 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
     6601 cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
     6661 tttcttagga cggatcgctt gcctgtaact tacacgcgcc tcgtatcttt taatgatgga
     6721 ataatttggg aatttactct gtgtttattt atttttatgt tttgtatttg gattttagaa
     6781 agtaaataaa gaaggtagaa gagttacgga atgaagaaaa aaaaataaac aaaggtttaa
     6841 aaaatttcaa caaaaagcgt actttacata tatatttatt agacaagaaa agcagattaa
     6901 atagatatac attcgattaa cgataagtaa aatgtaaaat cacaggattt tcgtgtgtgg
     6961 tcttctacac agacaagatg aaacaattcg gcattaatac ctgagagcag gaagagcaag
     7021 ataaaaggta gtatttgttg gcgatccccc tagagtcttt tacatcttcg gaaaacaaaa
     7081 actatttttt ctttaatttc tttttttact ttctattttt aatttatata tttatattaa
     7141 aaaatttaaa ttataattat ttttatagca cgtgatgaaa aggacccagg tggcactttt
     7201 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     7261 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     7321 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     7381 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     7441 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     7501 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     7561 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     7621 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     7681 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     7741 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     7801 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     7861 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     7921 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     7981 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     8041 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     8101 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     8161 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     8221 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     8281 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     8341 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     8401 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     8461 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     8521 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     8581 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     8641 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     8701 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     8761 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     8821 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     8881 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     8941 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     9001 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     9061 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     9121 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     9181 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caat
//
