LOCUS       sequence-502434-        9225 bp    DNA     circular SYN 10-MAR-2026
DEFINITION  FAP tagged STE3-K424R mutant (N-terminally tagged, optimized) under 
            the Ste3 promoter with 2xMYC tag and MFA1 signal sequence to help 
            target construct to the ER.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9225)
  AUTHORS   Oppenheimer KG, Hager NA, McAtee CK, Filiztekin E, Shang C, Warnick 
            JA, Bruchez MP, Brodsky JL, Prosser DC, Kwiatkowski AV, O'Donnell AF
  TITLE     Optimization of the fluorogen-activating protein tag for 
            quantitative protein trafficking and colocalization studies in S. 
            cerevisiae.
  JOURNAL   Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi: 10.1091/mbc.E24-04-0174. 
            Epub 2024 May 29.
  PUBMED    38809589
REFERENCE   2  (bases 1 to 9225)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 10, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell."; date: "2024-07-1"; volume: "35(7):mr5. doi"; pages: " 
            10.1091/mbc.E24-04-0174. Epub 2024 May 29"
COMMENT     Sequence Label: pRS415-STE3pr-MFA-Igkappa-FAPoptim-STE3-K424R
FEATURES             Location/Qualifiers
     source          1..9225
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..22
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(37..42,43..60,61..67)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    75..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     80..102
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     99..115
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     99..115
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     134..154
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        136..154
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     sig_peptide     928..987
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     CDS             1726..1755
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1756..1785
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      3232..3479
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(3248..3266)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(3497..3516)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3498..3516)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3526..3543)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3526..3542)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3535..3557)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3683..4138
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3770..3789)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3980..4001
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4439..4846
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             4847..5941
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(6435..6454)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6554..6576
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6614..6632)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    6674..7177
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        7214..7318
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7319..7387,7388..8179)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7537..7556)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8350..8938
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8839..8858
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9092..9109
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
       61 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      121 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tcatttacac
      181 caccagaagc gttctggctc cccttttctc taaacgttaa acattttaca attgaaatgt
      241 taccaatcct atattattgt accacattgc cagatttatg aactctgggt atgggtgcta
      301 attttcgtta gaagcgctgg tacaattttc tctgtcattg tgacactaat taggaaactt
      361 ctcgactatc aatgtgtaaa tgaaggaata atggcggaaa ctttgaaact ttgtcaataa
      421 ttgcatcatt ggatgcgttt catttggccg ttatcacgga gaggcagagt tctctccaca
      481 atttgggcag aagtcttttg aaaagacata tatatatata tatatgtata tgagtggatg
      541 cttaaggtaa gaataatttc tgaattccca agtattcatt ttgtgcagta ttcacatatt
      601 ctattttatt gctttttaac tttagaggca attaaatttg tgtaggaaag gcaaaatact
      661 atcaaaattt tctctagaat gagatttcct tcaattttta ctgcagtttt attcgcagca
      721 tcctccgcat tagctgctcc agtcaacact acaacagaag atgaaacggc acaaattccg
      781 gctgaagctg tcatcggtta cttagattta gaaggggatt tcgatgttgc tgttttgcca
      841 ttttccaaca gcacaaataa cgggttattg tttataaata ctactattgc cagcattgct
      901 gctaaagaag aaggggtatc tttggatatg gagacagaca cactcctgct atgggtactg
      961 ctgctctggg ttccaggttc cactggtgac actagtggat ccatgcaagc tgttgttact
     1021 caagaaccat ctgttactgt ttctccaggt ggtactgtta tcttgacttg tggttctggt
     1081 actggtgctg ttacttctgg tcactacgct aactggttcc aacaaaagcc aggtcaagct
     1141 ccaagagctt tgatcttcga cactgacaag aagtactctt ggactccagg tagattctct
     1201 ggttctttgt tgggtgctaa ggctgctttg actatctctg acgctcaacc agaagacgaa
     1261 gctgaatact actgttcttt gtctgacgtt gacggttact tgttcggtgg tggtactcaa
     1321 ttgactgttt tgtctggtgg tggtggttct ggtggtggtg gttctggtgg tggtggttct
     1381 ggtggtggtg gttctcaagc tgttgttact caagaaccat ctgttactgt ttctccaggt
     1441 ggtactgtta tcttgacttg tggttctggt actggtgctg ttacttctgg tcactacgct
     1501 aactggttcc aacaaaagcc aggtcaagct ccaagagctt tgatcttcga cactgacaag
     1561 aagtactctt ggactccagg tagattctct ggttctttgt tgggtgctaa ggctgctttg
     1621 actatctctg acgctcaacc agaagacgaa gctgaatact actgttcttt gtctgacgtt
     1681 gacggttact tgttcggtgg tggtactcaa ttgactgttt tgtctgagca gaagctgatc
     1741 agcgaggagg acctggagca gaagctgatc agcgaggagg acctggcggg tgcgggtgcg
     1801 ggtcgccccg ggatgtcata caagtcagca ataatagggc tttgtttgct agctgtgata
     1861 ctattagctc cccctttagc atggcattca cataccaaga atattccagc aatcattttg
     1921 ataacatggc ttcttacaat gaatttaacg tgtattgtag atgcggcaat atggagtgac
     1981 gacgatttcc tcacgagatg ggatggtaaa ggttggtgtg atattgtcat caagttgcag
     2041 gttggtgcga atattggcat atcatgtgcc gttaccaaca tcatttacaa cttgcataca
     2101 attttgaagg cagatagtgt tttaccggat ctttcatcat ggacgaaaat cgtcaaggac
     2161 cttgtgatta gcttgttcac acctgtcatg gtcatgggat tttcatatct gttacaagta
     2221 tttagatatg gtatcgctcg ttacaacggt tgccaaaact tattgtctcc gacatggatt
     2281 accactgttt tgtataccat gtggatgctt atatggtcat ttgtgggcgc tgtttatgcc
     2341 accttagtac tgttcgtgtt ttataaaaaa cgcaaggacg ttagggatat tttacactgt
     2401 accaattcag gtttaaacct gacaaggttc gcaaggctgt tgatattctg tttcattatt
     2461 attttagtca tgttcccttt ttctgtttac acctttgttc aagatttaca gcaggtagaa
     2521 ggacactata cttttaaaaa tacccattcc agcaccatct ggaataccat tattaaattt
     2581 gaccctggca gaccaattta taatatatgg ctttatgttt tgatgtctta cctagtattt
     2641 ctaatctttg gcttaggttc tgatgctttg catatgtact ctaaattcct gcgttccatc
     2701 aaactaggat ttgtacttga catgtggaaa agattcattg ataagaataa ggaaaaacga
     2761 gtaggcatat tgctaaacaa gctgtcctca cgcaaagaga gccgtaaccc attttctaca
     2821 gactctgaga actatatctc cacgtgtaca gaaaactatt ctccctgtgt aggtacacca
     2881 atatcacaag cgcatttcta tgtcgactat aggattccag atgatcctag aaaatctcaa
     2941 aataaaagca aaaaatattt gtttgctgat aaagaaacag atgatattct tgatgaaata
     3001 gacctaaaag aaagtaggca catcccttac gtcacgcaag gacagagctt tgacgacgaa
     3061 atatcacttg gaggattctc aagagttact ctcgattatt cagaaaagct tcataattct
     3121 gcaagctcca attttgaagg ggaaagtctt tgctactctc cagcttcaaa agaagagaat
     3181 tcaagctcaa acgaacatag ttcagaaaat actgcaggcc cttaactcga gtcatgtaat
     3241 tagttatgtc acgcttacat tcacgccctc cccccacatc cgctctaacc gaaaaggaag
     3301 gagttagaca acctgaagtc taggtcccta tttatttttt tatagttatg ttagtattaa
     3361 gaacgttatt tatatttcaa atttttcttt tttttctgta cagacgcgtg tacgcatgta
     3421 acattatact gaaaaccttg cttgagaagg ttttgggacg ctcgaaggct ttaatttgcg
     3481 gccggtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt
     3541 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
     3601 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
     3661 gcgcagcctg aatggcgaat ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     3721 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     3781 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     3841 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     3901 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     3961 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     4021 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     4081 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttcc
     4141 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tcgaccggtc
     4201 gaggagaact tctagtatat ctacatacct aatattattg ccttattaaa aatggaatcc
     4261 caacaattac atcaaaatcc acattctctt caaaatcaat tgtcctgtac ttccttgttc
     4321 atgtgtgttc aaaaacgtta tatttatagg ataattatac tctatttctc aacaagtaat
     4381 tggttgtttg gccgagcggt ctaaggcgcc tgattcaaga aatatcttga ccgcagttaa
     4441 ctgtgggaat actcaggtat cgtaagatgc aagagttcga atctcttagc aaccattatt
     4501 tttttcctca acataacgag aacacacagg ggcgctatcg cacagaatca aattcgatga
     4561 ctggaaattt tttgttaatt tcagaggtcg cctgacgcat ataccttttt caactgaaaa
     4621 attgggagaa aaaggaaagg tgagagcgcc ggaaccggct tttcatatag aatagagaag
     4681 cgttcatgac taaatgcttg catcacaata cttgaagttg acaatattat ttaaggacct
     4741 attgtttttt ccaataggtg gttagcaatc gtcttacttt ctaacttttc ttacctttta
     4801 catttcagca atatatatat atatatttca aggatatacc attctaatgt ctgcccctaa
     4861 gaagatcgtc gttttgccag gtgaccacgt tggtcaagaa atcacagccg aagccattaa
     4921 ggttcttaaa gctatttctg atgttcgttc caatgtcaag ttcgatttcg aaaatcattt
     4981 aattggtggt gctgctatcg atgctacagg tgttccactt ccagatgagg cgctggaagc
     5041 ctccaagaag gctgatgccg ttttgttagg tgctgtgggt ggtcctaaat ggggtaccgg
     5101 tagtgttaga cctgaacaag gtttactaaa aatccgtaaa gaacttcaat tgtacgccaa
     5161 cttaagacca tgtaactttg catccgactc tcttttagac ttatctccaa tcaagccaca
     5221 atttgctaaa ggtactgact tcgttgttgt cagagaatta gtgggaggta tttactttgg
     5281 taagagaaag gaagacgatg gtgatggtgt cgcttgggat agtgaacaat acaccgttcc
     5341 agaagtgcaa agaatcacaa gaatggccgc tttcatggcc ctacaacatg agccaccatt
     5401 gcctatttgg tccttggata aagctaatgt tttggcctct tcaagattat ggagaaaaac
     5461 tgtggaggaa accatcaaga acgaattccc tacattgaag gttcaacatc aattgattga
     5521 ttctgccgcc atgatcctag ttaagaaccc aacccaccta aatggtatta taatcaccag
     5581 caacatgttt ggtgatatca tctccgatga agcctccgtt atcccaggtt ccttgggttt
     5641 gttgccatct gcgtccttgg cctctttgcc agacaagaac accgcatttg gtttgtacga
     5701 accatgccac ggttctgctc cagatttgcc aaagaataag gtcaacccta tcgccactat
     5761 cttgtctgct gcaatgatgt tgaaattgtc attgaacttg cctgaagaag gtaaggccat
     5821 tgaagatgca gttaaaaagg ttttggatgc aggtatcaga actggtgatt taggtggttc
     5881 caacagtacc accgaagtcg gtgatgctgt cgccgaagaa gttaagaaaa tccttgctta
     5941 aaaagattct ctttttttat gatatttgta cataaacttt ataaatgaaa ttcataatag
     6001 aaacgacacg aaattacaaa atggaatatg ttcatagggt agacgaaact atatacgcaa
     6061 tctacataca tttatcaaga aggagaaaaa ggaggatgta aaggaataca ggtaagcaaa
     6121 ttgatactaa tggctcaacg tgataaggaa aaagaattgc actttaacat taatattgac
     6181 aaggaggagg gcaccacaca aaaagttagg tgtaacagaa aatcatgaaa ctatgattcc
     6241 taatttatat attggaggat tttctctaaa aaaaaaaaaa tacaacaaat aaaaaacact
     6301 caatgacctg accatttgat ggagtttaag tcaatacctt cttgaaccat ttcccataat
     6361 ggtgaaagtt ccctcaagaa ttttactctg tcagaaacgg ccttaacgac gtagtcgata
     6421 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg
     6481 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
     6541 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
     6601 gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     6661 gtttcttagg acggatcgct tgcctgtaac ttacacgcgc ctcgtatctt ttaatgatgg
     6721 aataatttgg gaatttactc tgtgtttatt tatttttatg ttttgtattt ggattttaga
     6781 aagtaaataa agaaggtaga agagttacgg aatgaagaaa aaaaaataaa caaaggttta
     6841 aaaaatttca acaaaaagcg tactttacat atatatttat tagacaagaa aagcagatta
     6901 aatagatata cattcgatta acgataagta aaatgtaaaa tcacaggatt ttcgtgtgtg
     6961 gtcttctaca cagacaagat gaaacaattc ggcattaata cctgagagca ggaagagcaa
     7021 gataaaaggt agtatttgtt ggcgatcccc ctagagtctt ttacatcttc ggaaaacaaa
     7081 aactattttt tctttaattt ctttttttac tttctatttt taatttatat atttatatta
     7141 aaaaatttaa attataatta tttttatagc acgtgatgaa aaggacccag gtggcacttt
     7201 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     7261 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     7321 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     7381 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     7441 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     7501 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     7561 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     7621 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     7681 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     7741 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     7801 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     7861 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     7921 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     7981 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     8041 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     8101 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     8161 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     8221 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     8281 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     8341 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     8401 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     8461 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     8521 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     8581 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     8641 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     8701 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     8761 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     8821 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     8881 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     8941 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     9001 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     9061 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     9121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     9181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaat
//
