LOCUS       sequence-502532-        8308 bp    DNA     circular SYN 11-MAR-2026
DEFINITION  SpmX△TM-mRFP-PopZ, sfGFP-PodJ249pse.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8308)
  AUTHORS   Chou TW, Huang IJ, Hsu YC, Wu SH, Hong JC, Ko YH, Su TR, Jiang JR, 
            Huang HC
  TITLE     Construction and phenotypic classification of synthetic dual-pole 
            Escherichia coli cells.
  JOURNAL   Commun Biol. 2025 Jul 20;8(1):1078. doi: 10.1038/s42003-025-08495-w.
  PUBMED    40684034
REFERENCE   2  (bases 1 to 8308)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 11, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2025-07-20"; volume: "8(1):1078. doi"; pages: " 
            10.1038/s42003-025-08495-w"
COMMENT     Sequence Label: S'RP + PodJ'
FEATURES             Location/Qualifiers
     source          1..8308
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      2..59
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(83..100)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(254..842)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(334..353)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1025..1119)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1140..1799)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1714..1733
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1961..1979
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1983..2026)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      complement(2057..2084)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(2100..2171)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(2190..2207)
                     /codon_start=1
                     /product="tetracysteine peptide that binds biarsenical 
                     labeling reagents"
                     /label=tetracysteine tag
                     /translation="CCPGCC"
     CDS             complement(2763..3437)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     RBS             4515..4526
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     promoter        complement(4538..4572)
                     /label=BBa_J23116
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.16"
     terminator      complement(4608..4637)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     CDS             complement(4700..5782)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     5744..5763
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        join(5917..5922,5923..5938,5939..5945)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    5951..5970
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     RBS             5989..6000
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             join(6009..6011,6012..6722)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             6009..6722
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(6039..6067)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     6662..6682
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      8159..8230
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      8246..8273
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 gtccggcaaa aaagggcaag gtgtcaccac cctgcccttt ttctttaaaa ccgaaaagat
       61 tacttcgcgt tatgcaggct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
      121 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
      181 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
      241 cgttgctggc gtttttccac aggctccgcc cccctgacga gcatcacaaa aatcgacgct
      301 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
      361 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
      421 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
      481 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
      541 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
      601 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
      661 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
      721 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
      781 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
      841 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
      901 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
      961 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agctcgaggc
     1021 ttggattctc accaataaaa aacgcccggc ggcaaccgag cgttctgaac aaatccagat
     1081 ggagttctga ggtcattact ggatctatca acaggagtcc aagcgagctc gatatcaaat
     1141 tacgccccgc cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca
     1201 tggaagccat cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg
     1261 ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc
     1321 acgtttaaat caaaactggt gaaactcacc cagggattgg ctgagacgaa aaacatattc
     1381 tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa
     1441 tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt
     1501 tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca
     1561 ccgtctttca ttgccatacg aaattccgga tgagcattca tcaggcgggc aagaatgtga
     1621 ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata
     1681 tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt
     1741 tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt
     1801 ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt
     1861 atttcattat ggtgaaagtt ggaacctctt acgtgcccga tcaactcgag tgccacctga
     1921 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggca
     1981 gaatttcaga taaaaaaaat ccttagcttt cgctaaggat gatttctgga attccggctc
     2041 tagagcagta tataaacgca gaaaggccca cccgaaggtg agccagtgtg actctagtag
     2101 agagcgttca ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt
     2161 cgttttattt gatgcctggc tctagtttaa caacatcctg gacaacaggc gccgcgtccc
     2221 cgagagatac gctgcacttc ttcctcaacc ttggtctcga cgatgcgcgg caggttctgg
     2281 tccagccact ccttgagcag cgggcgcagc agctcgcgta cgacgtcttc cagcgtccga
     2341 ccgtccttgg gcatcagcag ggccgagctc aggctgccga aggcgctcgc cgcggccgaa
     2401 gcggccgaaa cgccgaccag ctgctcggcg acttcgtcgc gatcaaacac cggagccgcc
     2461 ggcggcggcg tgtaggccgg ttccgactca ggttccggcg gcgaatagac gtcgatgtcg
     2521 ccgacagtct ccagcggcgg cagctcgggc tcgggcgcga tcgggtccgt caattccaga
     2581 acctcgtcgt cgaacgacac cggttcaggt tcgggttccg gcggcggcgg gggcgccgct
     2641 tcggccgcag gctccgccgg cgcgtcatcc tccgagatga tgcgtcgaat ggaggcgagg
     2701 atttcctcca ttgtaggttc ttgagactga tcggacatgg atcccgatga gccagatgat
     2761 ccagcaccgg tggagtgacg accttcagca cgttcgtact gttcaacgat ggtgtagtct
     2821 tcgttgtggg aggtgatgtc cagtttgatg tcggttttgt aagcacccgg cagctgaacc
     2881 ggttttttag ccatgtaggt ggttttaact tcagcgtcgt agtgaccacc gtctttcagt
     2941 ttcagacgca ttttgatttc acctttcaga gcaccgtctt ccgggtacat acgttcggtg
     3001 gaagcttccc aacccatggt ttttttctgc ataaccggac cgtcggacgg gaagttggta
     3061 ccacgcagtt taactttgta gatgaactca ccgtcttgca gggaggagtc ctgggtaacg
     3121 gtaacaacac caccgtcttc gaagttcata acacgttccc atttgaaacc ttccgggaag
     3181 gacagtttca ggtagtccgg gatgtcagcc gggtgtttaa cgtaagcttt ggaaccgtac
     3241 tggaactgcg gggacaggat gtcccaagcg aacggcagcg gaccaccttt ggtaactttc
     3301 agtttagcgg tctgggtacc ttcgtacgga cgaccttcac cttcaccttc gatttcgaac
     3361 tcgtgaccgt taacggaacc ttccatacga actttgaaac gcatgaactc tttgataacg
     3421 tcttcggagg aagccattga ggcctttcct gtgtggaagt ccatcacgcc gacggttgcg
     3481 tcctcgctgg tcgagaagcc gtccagatta aagaccgacg agccggtggc cggcgcgccg
     3541 aacagggtcg gctcgctcgg cgcgggacgc gtcggggccg gcgcctcgac cggagcctcg
     3601 gcctgttcag aagcttgtgg cgtcaggcgg aacggcgtga actcgaccgg agcggccggc
     3661 ggcggttcga ccggcgcggg ctgcggctcg gcctcgaaca gcaccggcgc cggctccatc
     3721 gcggcaggtt gcgtcggggc cggcggttcg ggcaggccca gatcatcctg gcgcatggcc
     3781 ggcttggcca tcgacgcggg cgccgacggc gtttccggca agatcgcttc caggcgcgca
     3841 gcgaccgcag cggccgattg ctcggtggcg gtcggagcgt cctcttccgg gacgaccacg
     3901 ttcagcggca ggccttcctc gcgctgggcg atgacacggt cgccttcggt gcgggtgttg
     3961 acggtggccg gcgtctgctt gggcaccgcg cagctggcgt catagtcgac cttgggacgc
     4021 agcacggggc tgggcgcggg gacccattcg ccgttggccg gcgtgaggaa cagagtcttc
     4081 tcggcggagc ggcggcgaac cagtgcatcg atgacgatgc gctcgccctc gaaatcggcc
     4141 ttgcgccaca tctccatcgc gcacgcggct tgcagcagcg agccctcgtt gatccggcgc
     4201 aggacgcccg agcggaggaa gttgtcgagg ccgatattga aggcgaagca caccaacgcg
     4261 tcgaactggt tctggttcag cggcgtgtag gtgtgctcgt tcaccgaatg ggcgaccgag
     4321 atgagatcat agagcagcag cgcctcggcg tccttttccg agacagaggc gccctcgcgg
     4381 gcggtcaggg tgtggccata gccgaccgtc cagcgcccgt cggggagctg cgcggccttc
     4441 tgacggtacc cttcgaatct cttgatgaga tcgacggcgg cccgggagac ctgatgacgc
     4501 ggtttcatct agtatttctc ctctttaatc tctagtagct agcatagtcc ctaggactga
     4561 gctagctgtc aactctcgga ctagaatatt cagcaatttg cccgtgccga agaaaggccc
     4621 acccgtgaag gtgagccagt gagttgattg ctacgtaatg tcggccaatt cgcgctaact
     4681 tacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     4741 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gccagggtgg
     4801 tttttctttt caccagtgag actggcaaca gctgattgcc cttcaccgcc tggccctgag
     4861 agagttgcag caagcggtcc acgctggttt gccccagcag gcgaaaatcc tgtttgatgg
     4921 tggttaacgg cgggatataa catgagctat cttcggtatc gtcgtatccc actaccgaga
     4981 tatccgcacc aacgcgcagc ccggactcgg taatggcgcg cattgcgccc agcgccatct
     5041 gatcgttggc aaccagcatc gcagtgggaa cgatgccctc attcagcatt tgcatggttt
     5101 gttgaaaacc ggacatggca ctccagtcgc cttcccgttc cgctatcggc tgaatttgat
     5161 tgcgagtgag atatttatgc cagccagcca gacgcagacg cgccgagaca gaacttaatg
     5221 ggcccgctaa cagcgcgatt tgctggtgac ccaatgcgac cagatgctcc acgcccagtc
     5281 gcgtaccgtc ctcatgggag aaaataatac tgttgatggg tgtctggtca gagacatcaa
     5341 gaaataacgc cggaacatta gtgcaggcag cttccacagc aatggcatcc tggtcatcca
     5401 gcggatagtt aatgatcagc ccactgacgc gttgcgcgag aagattgtgc accgccgctt
     5461 tacaggcttc gacgccgctt cgttctacca tcgacaccac cacgctggca cccagttgat
     5521 cggcgcgaga tttaatcgcc gcgacaattt gcgacggcgc gtgcagggcc agactggagg
     5581 tggcaacgcc aatcagcaac gactgtttgc ccgccagttg ttgtgccacg cggttgggaa
     5641 tgtaattcag ctccgccatc gccgcttcca ctttttcccg cgttttcgca gaaacgtggc
     5701 tggcctggtt caccacgcgg gaaacggtct gataagagac accggcatac tctgcgacat
     5761 cgtataacgt tactggtttc atattcacca ccctgaattg actctcttcc gggcgctatc
     5821 atgccatacc gcgaaaggtt ttgcgccatt cgatggcgcg ccgcttcgtc aggccacata
     5881 gctttcttgt tctgatcgga acgatcgttg gctgtgttga caattaatca tcggctcgta
     5941 taatgtgtgg aattgtgagc gctcacaatt ctatggacta tactagagaa agaggagaaa
     6001 tactagagat gagcaaagga gaagaacttt tcactggagt tgtcccaatt cttgttgaat
     6061 tagatggtga tgttaatggg cacaaatttt ctgtccgtgg agagggtgaa ggtgatgcta
     6121 caaacggaaa actcaccctt aaatttattt gcactactgg aaaactacct gttccgtggc
     6181 caacacttgt cactactctg acctatggtg ttcaatgctt ttcccgttat ccggatcaca
     6241 tgaaacggca tgactttttc aagagtgcca tgcccgaagg ttatgtacag gaacgcacta
     6301 tatctttcaa agatgacggg acctacaaga cgcgtgctga agtcaagttt gaaggtgata
     6361 cccttgttaa tcgtatcgag ttaaagggta ttgattttaa agaagatgga aacattcttg
     6421 gacacaaact cgagtacaac tttaactcac acaatgtata catcacggca gacaaacaaa
     6481 agaatggaat caaagctaac ttcaaaattc gccacaacgt tgaagatggt tccgttcaac
     6541 tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt ttaccagaca
     6601 accattacct gtcgacacaa tctgtccttt cgaaagatcc caacgaaaag cgtgaccaca
     6661 tggtccttct tgagtttgta actgctgctg ggattacaca tggcatggat gagctctaca
     6721 aatgttttca cacaggaaag gcctcgatga ctgctgcttc gccatggagc gttaagggga
     6781 ttgaccccaa ggcacgggag gtcgcgaaag acctcgcgcg tcgctccggc atgactttgg
     6841 gtgaatggct gaaccgaatg atcatcgaag gcgatggtca gaccgctgat ccgcgtctcg
     6901 cgggggacga tgtccccaac cgcgcctatc tcgagattgt caaagatgat gcgccgccgc
     6961 gcatcgagat cgccgagcat ccggcagatg aggttggtcg cgtcgcgctg gcgctggatc
     7021 gcctgaccca acgcattgaa gccgccgagg ggcgcaacgc cgccgccatc accggcatcg
     7081 atcactccgt ccgtgacgcc ctgacgcgtc tgggcgcatc cgaacgtgag cagatcgccg
     7141 tcgccgcccg gttcgagggc gctgtcgacg aactgaagac cgagcaggct cgggccaccg
     7201 aacgtctgcg ccggatcgag agcgaggcgg cgggtccgcg ctcggccgag gcgttgcgcg
     7261 cgcttgaagg cgccttgggc aaggtcgccg gacaccttta cgaaggcgag gcgcggacgc
     7321 gcgaggcgat cgcgaccctg gaagccaagc tgaaccagca gagctcgggc gatcccagcg
     7381 cgctggtcga ggctgtggtc gcgcgactgg gcgaacgcct cgaagccgcc gaaacccgca
     7441 ccagcgacgc gctgcgcgaa ctgggcgcgt cgttccaggc cctggatcag cgccaggaac
     7501 tggtcgaccg catccgccag agcgaagagc gcacgctccg catgctggaa gaagcgcgcg
     7561 agaagatcga cagccgtctg tccgaggcgc agcggaagct ggaagctgcg ccgccgtcgc
     7621 cgccgcccgc gcaggctccg gcgccggttg cgactgctca gcgtccggtt ccgccggcgg
     7681 cttcgccctt cgaggacaac tatttcagcc aggccgcctc gttcagcacg tctgaggatg
     7741 aggctgacgc gtttgatgcg ccgcctgcgc cggcccgtag cttcgaggtg gcggagttcc
     7801 cggctgcgga gccggaagaa ccggccttcg cacacgatga ctacgcgatc gccgatggct
     7861 tcgagccgga aagcccgcgc tatgaagtcg agcctgaggt ctccgacttc gcgcccgccg
     7921 agccgtcgcg tccgatgtcg acccgcgata tcatcgagca ggcccgcgcc gccgcgcgcg
     7981 ccgccgccgc ctcggaaggc aagggcggca aggccaagtc ggcgaagaag gaaaaggctt
     8041 ccaaggccag cggctcgctg ttcagcggct ttggcggttt ctcgaccaag aagtcgaagg
     8101 cgcgcttggg cgcgaccgtg acgacggcct aataactagg gcccataccc ccaggcatca
     8161 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt
     8221 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttatac
     8281 gacgtacgga ctagtagcgg ccgctgca
//
