LOCUS       sequence-502549-        2342 bp    DNA     circular SYN 11-MAR-2026
DEFINITION  GoldenBraid Alpha2 vector with f1 ori removed and additional BsaI 
            sites in lacZ to reduce background..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2342)
  TITLE     Drosophila GoldenBraid plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 11, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Alpha2x
FEATURES             Location/Qualifiers
     source          1..2342
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        47..94
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        148..166
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     148..165
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(219..238)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(220..238)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(245..262)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(245..261)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(254..276)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(470..489)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(621..1209)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(701..720)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        1324..1426
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1427..2242
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             1427..2242
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1500..1519)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 caaacatgat gtaacaagct tcgtctcagt caggagagag acccctgttg acaattaatc
       61 atcggcatag tatatcggca tagtataata cgacaaggtg aggaactaaa cccaggaggc
      121 agatcatgac catgattacg ccaagctatt taggtgacac tatagaatac tcaagcttgc
      181 atgggtctcg aggtcactct tgcgagacct cgaattcgcc ctatagtgag tcgtattaca
      241 attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
      301 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
      361 atcgcccttc ccaacagttg cgcagcctga atggcgaatg acgcctgatg cggtattttc
      421 tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct
      481 ctgatgccgc atagggtctc tcgcttgaga cgaagcttga cacggttatc cacagaatca
      541 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
      601 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
      661 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
      721 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
      781 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
      841 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
      901 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
      961 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     1021 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     1081 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     1141 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     1201 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     1261 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatccttttg
     1321 tgatgatcgg cacgtaagag gttccaactt tcaccataat gaaataagat cactaccggg
     1381 cgtatttttt gagttatcga gattttcagg agctaaggaa gctaaaatga gccatattca
     1441 acgggaaacg tcttgctcaa ggccgcgatt aaattccaac atggatgctg atttatatgg
     1501 gtataaatgg gctcgcgata atgtcgggca atcaggtgcg acaatctacc gattgtatgg
     1561 gaagcccgat gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt
     1621 tacagatgag atggtcagac taaactggct gacggaattt atgcctcttc cgaccatcaa
     1681 gcattttatc cgtactcctg atgatgcatg gttactcacc actgcgatcc cagggaaaac
     1741 agcattccag gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc
     1801 agtgttcctg cgccggttgc attcgattcc tgtttgtaat tgtcctttta acagcgatcg
     1861 cgtatttcga ctcgctcagg cgcaatcacg aatgaataac ggtttggttg atgcgagtga
     1921 ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataaact
     1981 tttgccattc tcaccggatt cagtagtcac tcatggtgat ttctcacttg ataaccttat
     2041 ttttgacgag gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg
     2101 ataccaggat cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa
     2161 acggcttttt caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcattt
     2221 gatgctcgat gagtttttct gatttttttt aaggcagtta ttggtgccct taaacgcctg
     2281 gttgctacgc ctgaataagt gataataagc ggatgaatgg cagaaattcg atgataagct
     2341 gt
//
