LOCUS       sequence-502550-        8807 bp    DNA     circular SYN 11-MAR-2026
DEFINITION  Drosphila y+ gene in GoldenBraid Alpha2 vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8807)
  TITLE     Drosophila GoldenBraid plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8807)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 11, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: y.Alpha2
FEATURES             Location/Qualifiers
     source          1..8807
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             5258..5269
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     rep_origin      complement(6617..7205)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6697..6716)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        7320..7422
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             7423..8238
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             7423..8238
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(7496..7515)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      8352..8780
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8439..8458)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8649..8670
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 taacaagctt cgtctcagtc aggagattat cgcccgatta ccacattgag tggtttaaaa
       61 tagccataaa atatgcaact gacgatggct taagataaat acgtcgcaga gtcactcata
      121 aatttcgaac gcagcccgct gatttaccta cccctctaaa cgattcatag tatatgtacg
      181 agtatatcca ctaagctttt tcgagcactg attttttcgc ttgcacgaga caagtgcacc
      241 accgcaattg caggcaaatt atgtctgagg taatgattcc gtttcgtgca agattacaca
      301 gaaatcaaat tacgacaacc tttattcagt aagcaaacaa agcctttgtt ggcatctaat
      361 tattccactt atggttgcga tttcgggagc tacaatcggt tttggtttag tatatctagc
      421 gagttccttg gcgacattta aaatttacaa ataaagtttc tctattcaat cgggacagtg
      481 gaaattgact attttattta tattaatgaa cttattttta atttggctta agttactaag
      541 gggtactaat agtttgagcg cagtgcatgt catggggaca tgtgcaattg tgtgtaagcg
      601 ggaagtgatc gcggccttcc gaatttggcc atgccaaata atcccagctc gaaaggaggg
      661 gacccggcgg tcagggccct ggacattgaa cttgaaaaaa aaaaacacaa aaatatataa
      721 cacaaaacgg aaaatgctgt gtaccgctta tgttagagaa gttgagcaac gggtttttcg
      781 ttttgcagtc acgatggatt tccaaattag tgtaggaggg gggaggggag ggagggagat
      841 aatgtccagg ctgccataag tggggaataa ggaaaataaa acatgaaaca cgggtcgggc
      901 aatgtcatgc ggtattcggc tttgctttcc gcccaagttg aagtgatcct gtgtgtaaat
      961 aatgtcgaat gttgccggtc ggttgcataa gcgttagtca attatgccaa agagatctga
     1021 tttgtggaag ctttttttga ccacttagcg cgctccgctt gatgttgttt tgttttgtgc
     1081 tggggcagaa aacttgtttc aattattggg aaaagtgcgt ataaatcatt gccgcaagct
     1141 ctgaaaagcg aaaaagaaaa acagtaacca aacagacaaa cgcagcatcc ccccacacaa
     1201 ttaagcaaaa acttgaaaac aagtcaattc gaaaaaaatt ataggttcaa cggctgcagc
     1261 gatcgcatca ttagttgcgt ttttagtaaa tacaccattt cattacacaa cacacacaat
     1321 taattaataa aactgtactg gttatttcaa gtgtgtcttt taataagcct gccgatcgca
     1381 ataaattcga gcagcattgc cggtaatttt gtgcaacata tttttcgatt gccacaccgt
     1441 gtttgtttat ttttctgtgg gtgcaatgat ttagaatgcg ggcaagggat caagttgaac
     1501 cacttctaag aaaaaatagc attgcataaa tgatatagag tccaaaaact acacaaattc
     1561 aatagcagta atggttacat tagctttgaa attgttttta gacatccgaa gaaataagat
     1621 taaatttaaa cggcattctt taatttgtat tttaatattt tgagaggttt tccttattta
     1681 aagtgtagat tattgaggat taatgcaaac cactttatct gcggaggtcg taaaacgtat
     1741 ttttacccat ttgcatgttt attatgcgtg tggctggttg cattacttta cttaagtttt
     1801 gcaatttttt ctttagcaag caggtgcatt tgggccaaga gatatatgcg atcgctttcg
     1861 gttcgaattt tcaacattta cttgcggcta tggtcattag agcattaccc acttagggca
     1921 cccccaacat ccagttgatt ttcagggacc acaatatttt aaataacagc tagtggaatt
     1981 acctaaaagc gctttcgtcc cttttgaaat tttatgtaac actcaattat atttatgtat
     2041 atgtatgctc aaaatcacct gccaataact agcggaaacc aaatatttga ccctcagtga
     2101 attgtgaatc atcggtgacg cccaatcgaa atccaatcct aagcaattga aacgagcacg
     2161 agttccaatt taatagtata caaggaaaca cctgctttaa atactctaca tagtacacgt
     2221 tataataacg atttatttga tatttctgga tttttgtctg catgtatttc atataatatt
     2281 gatttgattt ttttaatgaa ttgaactaaa aaatcatatt agaacatttt ttgcagtcgc
     2341 cgataaagat gaacactgtt ctcagaacac aactgtcatg tattaagctt tcagattttc
     2401 agaaatttgg agagcaatgc attctatgca cgagcctcct ggccttacaa tttacttgtt
     2461 tgaaattaga tcgtcaaata aagtccctaa aattaaataa atagtagtca caactttaaa
     2521 ataggtctta atcttttagg gtaccgaaag gtatttcggc acaaatcagc gcagttttaa
     2581 atgtcgatga aggccaaaaa tcataccaaa cccagcgaaa ggtgatgtct gactcattaa
     2641 attgggggat tcgagtgtat ttattaaaca tgcgtgaaaa tcaatcatgg aagacaaaac
     2701 gcaaagttgg ccgatctatg ggaacagcat aagccacctg attacccgaa cactgaacca
     2761 cccgaatcac taaaaccacc gaagttggcg cgcgccttcg ttttcatttt cattggcctg
     2821 tcttcgtctt cggagaaaaa aaacttcata taaaacgcgg ccgacatatt atggccacca
     2881 gtcggtaccg cgccacggtc cacagaagag gattaaaaaa atatcacaca gccgaaggct
     2941 agagaagaac cccctatagc tgaacatata taaacaaata tatttttttt tattgccaac
     3001 acactttggc ttaagtgtta agagtgattg tcagcttaga gctaagtgca atgttccagg
     3061 acaaagggtg gatccttgtg accctgatca ccttggtgac gccgtcttgg gctgcttaca
     3121 aacttcagga gcgatatagt tggagccagc tggactttgc tttcccgaat acccgactaa
     3181 aggaccaagc tctggctagt ggagattata ttccgcaaaa tgctctacct gttggagtcg
     3241 aacactttgg caatcggtta ttcgtcactg ttccccgctg gcgtgatggt aagtggaagt
     3301 taaatatgaa gcccttgggg agatcgtaaa tgggacattc ttacttaggg catcagagat
     3361 atctgattga gtggttgaca gttttatatg gcttgtttga catgatgtaa aaacacaaaa
     3421 ttcatttagt ttaggtattc aaataagagc ttgttattta ttttagaatt tggagaacat
     3481 ttttttgtct ttctaccctt cttagaaaat aatattgttt tgtacaattt aattttaaac
     3541 tagtacagac gaaaaatgta tttttttatt tgtatgcctt ttcaccattt tggcagagga
     3601 ataaatatga caatatattt tgagagcacc ctcatgtaaa ggttttagcg tggcgacctc
     3661 tcataaatcc ggttggtacc cgcgcgttat tttaacattt taaacaatta accgttgtaa
     3721 aatcgaagcc aatagcatgg cattggcttt atactgtatt aaattgtatt atattaccat
     3781 ccgaattgta aagacttctt cagggccgcc acatagaaat ggaaatccaa tcacaaacaa
     3841 taacttatgg cattagctat taaccgacga ttagctgtca gttcaacaaa tgtaagagtg
     3901 gcgaaatgtt taaatgcgaa ggcattgttc tgtgactcac gttttattat taatcacaca
     3961 aatgattttg cttcaaaatt atttggctta cacaataatc aaaattttta tgaaatagtt
     4021 gaaacacaaa actaggaaat tttaaaaagc aatgaaacta aaaaacccaa ttgctaagat
     4081 tatatgatgc gcatacaaat acttcagtac gtctaggaat gctttcgatg attgattagt
     4141 ttttatgcat ggcttacaat tggtatttac atcgataaaa gctatatttt ctggtcgttg
     4201 cagtctcact cgcctgctat aaaaacatta aaagttccca gaaacaataa atgtctttaa
     4261 attcaattaa cgaagaaata aagaaggaaa agaactggag cggaaatcgg tcgaaatact
     4321 gccaatggcc acatatacat ttaacagcga tatatggtat acatattgat aatgatgtca
     4381 gacgcaattg cttcagacgg ctaatgacat cgcaaattgc acgcaacttg caatagtgcc
     4441 aattatgact gaagtacata tagccgggga tcttttaaca taaacttcca gtagatgtac
     4501 aagcagaaaa aagagccatt agcacggcag ttaccattgc ttatgattcc ttgtgtccaa
     4561 aataatgaca aataggtata taaataatta aatgccaaac ataagcgatt ctaatttacc
     4621 tttacatctg tatgcattta catattatcc agaaaacaga cagcgataac ttgcaacatt
     4681 gcttagtata ataatccaaa gaaggaattt aggcagaaat tccagttaat taaatattca
     4741 aaacaaactt tatttagtgc ctcaataata gtttggccct gctaattctc ctattttatt
     4801 ttttagggat tccggccact ctgacctata taaacatgga ccgcagtttg acgggttcac
     4861 cggagctaat tccgtatcca gattggcgct caaatacagc tggagattgc gccaacagta
     4921 ttaccactgc ctaccgcatt aaagtggatg agtgtggtcg gctgtgggtt ttggacactg
     4981 gtaccgtagg catcggcaat accaccacta atccgtgccc ctatgcggta aatgtctttg
     5041 acttgaccac ggatacgcga attcggagat acgagctacc tggcgtggac acaaatccaa
     5101 atactttcat agctaacatt gccgtggata taggcaaaaa ttgcgatgat gcatatgcct
     5161 attttgccga tgaattggga tacggcttga ttgcttactc ctgggaactg aacaagtcct
     5221 ggagattctc ggcacattcg tattttttcc ccgatccatt gaggggcgat ttcaatgtcg
     5281 ctggtattaa cttccaatgg ggcgaggagg gtatatttgg tatgtccctt tcgcccattc
     5341 gatcggatgg ttatcgtacc ctgtacttta gtccgttagc aagtcatcga caatttgccg
     5401 tatccacgag gattttgagg gatgaaacca ggacggaaga tagctatcat gactttgttg
     5461 ccttagatga acggggtcca aactcccata ccacttcacg tgtgatgagc gatgatggaa
     5521 ttgagctgtt caatttaata gatcaaaatg cagtgggttg ctggcactca tcaatgccgt
     5581 actcaccgca atttcatggc attgtggatc gcgatgacgt tggcttagtt tttccggccg
     5641 atgtgaaaat tgatgagaac aaaaacgttt gggttctatc cgataggatg cccgttttct
     5701 tgctgtctga cttggattat tcagatacta atttccgaat ttacacggct cccttggcca
     5761 ctttaattga gaatactgtg tgtgatttga ggaataacgc ctatgggccg ccaaataccg
     5821 tttcaatacc aaaacaagcc gttttgccaa tgggtccacc gttatatacg aaacaatatc
     5881 gtcctgtctt gccacagaaa cctcagacca gctgggcttc ctcgccgcct cctccaagtc
     5941 gcacttattt gcccgccaat tcaggcaatg tagtctccag tattagtgtc tctacaaatt
     6001 ctgtgggtcc tgcaggagtg gaggtgccaa aggcctatat tttcaaccag cacaacggca
     6061 taaattacga gacaagtggt ccccatctat ttcccaccca tcaacccgcc caaccgggtg
     6121 gccaggatgg tgggttaaaa acttatgtga atgcccgcca atctgggtgg tggcatcatc
     6181 agcatcaagg ttaagcttca taatcctaca cacggtactt gggtatattc tcacacactc
     6241 gattgatgta aagaatattt aaagacaaca acatagggca acagcggtta aaaaaaccac
     6301 atgacgtatg agcaagtggc aaatcaatac tttatctagt tatgttaagc aaaaaataac
     6361 aataaatcaa cttttttttg aaggttaaga gtttacgcaa ttttcttgag cggaaaaagc
     6421 ggaaaaaatg taagtatgca taaattctaa atatatcaac aactgtacat tttctggagt
     6481 actactacca ggcaagacgc ttgagacgaa gcttgacacg gttatccaca gaatcagggg
     6541 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     6601 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     6661 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     6721 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     6781 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     6841 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     6901 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     6961 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     7021 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     7081 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     7141 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     7201 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     7261 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttgtgat
     7321 gatcggcacg taagaggttc caactttcac cataatgaaa taagatcact accgggcgta
     7381 ttttttgagt tatcgagatt ttcaggagct aaggaagcta aaatgagcca tattcaacgg
     7441 gaaacgtctt gctcaaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat
     7501 aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctaccgatt gtatgggaag
     7561 cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca
     7621 gatgagatgg tcagactaaa ctggctgacg gaatttatgc ctcttccgac catcaagcat
     7681 tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatcccagg gaaaacagca
     7741 ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagtg
     7801 ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc cttttaacag cgatcgcgta
     7861 tttcgactcg ctcaggcgca atcacgaatg aataacggtt tggttgatgc gagtgatttt
     7921 gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca taaacttttg
     7981 ccattctcac cggattcagt agtcactcat ggtgatttct cacttgataa ccttattttt
     8041 gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac
     8101 caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg
     8161 ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt tcatttgatg
     8221 ctcgatgagt ttttctgatt ttttttaagg cagttattgg tgcccttaaa cgcctggttg
     8281 ctacgcctga ataagtgata ataagcggat gaatggcaga aattcgatga taagctgtca
     8341 aacatgatgt tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     8401 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     8461 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     8521 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     8581 acgtagtggg ccagcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     8641 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     8701 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     8761 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaattt
//
