LOCUS       sequence-502551-        2341 bp    DNA     circular SYN 11-MAR-2026
DEFINITION  GoldenBraid Alpha1 vector with f1 ori removed and additional BsaI 
            sites in lacZ to reduce background..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2341)
  TITLE     Drosophila GoldenBraid plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2341)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 11, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Alpha1x
FEATURES             Location/Qualifiers
     source          1..2341
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        42..89
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        143..161
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     143..160
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(214..233)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(215..233)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(240..257)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(240..256)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(249..271)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(465..484)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(620..1208)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(700..719)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        1323..1425
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1426..2241
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             1426..2241
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1499..1518)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 caaacatgat gtaacgaatt cgtctcagga gagagacccc tgttgacaat taatcatcgg
       61 catagtatat cggcatagta taatacgaca aggtgaggaa ctaaacccag gaggcagatc
      121 atgaccatga ttacgccaag ctatttaggt gacactatag aatactcaag cttgcatggg
      181 tctcgaggtc actcttgcga gacctcgaat tcgccctata gtgagtcgta ttacaattca
      241 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
      301 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
      361 ccttcccaac agttgcgcag cctgaatggc gaatgacgcc tgatgcggta ttttctcctt
      421 acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat
      481 gccgcatagg gtctctcgct gtcatgagac gaattctgac acggttatcc acagaatcag
      541 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
      601 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
      661 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
      721 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
      781 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
      841 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
      901 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
      961 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     1021 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     1081 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     1141 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     1201 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     1261 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttgt
     1321 gatgatcggc acgtaagagg ttccaacttt caccataatg aaataagatc actaccgggc
     1381 gtattttttg agttatcgag attttcagga gctaaggaag ctaaaatgag ccatattcaa
     1441 cgggaaacgt cttgctcaag gccgcgatta aattccaaca tggatgctga tttatatggg
     1501 tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctaccg attgtatggg
     1561 aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt
     1621 acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc gaccatcaag
     1681 cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc agggaaaaca
     1741 gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca
     1801 gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc
     1861 gtatttcgac tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat
     1921 tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataaactt
     1981 ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt
     2041 tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga
     2101 taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa
     2161 cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg
     2221 atgctcgatg agtttttctg atttttttta aggcagttat tggtgccctt aaacgcctgg
     2281 ttgctacgcc tgaataagtg ataataagcg gatgaatggc agaaattcga tgataagctg
     2341 t
//
