LOCUS       sequence-502628-        2205 bp    DNA     circular SYN 11-MAR-2026
DEFINITION  Constitutive promoter in B. subtilis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2205)
  TITLE     Ellis genetic toolkit
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 11, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: STK030_pSTK-0A-PliaG
FEATURES             Location/Qualifiers
     source          1..2205
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(29..688)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     603..622
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     850..868
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(872..915)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    918..937
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     terminator      1096..1153
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1177..1194)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1348..1936)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1428..1447)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(join(2119..2205,1..8))
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 caggagtcca agcgagctcg atatcaaatt acgccccgcc ctgccactca tcgcagtact
       61 gttgtaattc attaagcatt ctgccgacat ggaagccatc acaaacggca tgatgaacct
      121 gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc atggtgaaaa
      181 cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg aaactcaccc
      241 agggattggc tgacacgaaa aacatattct caataaaccc tttagggaaa taggccaggt
      301 tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt
      361 ggtattcact ccagagcgat gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag
      421 ggtgaacact atcccatatc accagctcac cgtctttcat tgccatacga aattccggat
      481 gagcattcat caggcgggca agaatgtgaa taaaggccgg ataaaacttg tgcttatttt
      541 tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta taggtacatt
      601 gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat atatcaacgg
      661 tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa aatctcgata
      721 actcaaaaaa tacgcccggt agtgatctta tttcattatg gtgaaagttg gaacctctta
      781 cgtgcccgat caactcgagt gccacctgac gtctaagaaa ccattattat catgacatta
      841 acctataaaa ataggcgtat cacgaggcag aatttcagat aaaaaaaatc cttagctttc
      901 gctaaggatg atttctggaa ttcgcggccg cttctagact gaggtctcaa gagcaaaaat
      961 cagaccagac aaaagcggca aatgaataag cggaacgggg aaggatttgc ggtcaagtcc
     1021 ttcccttccg cacgtatcaa ttcgcaagct tttcctttat aatagaatga atgaacatag
     1081 agacccctcc tgcagtccgg caaaaaaggg caaggtgtca ccaccctgcc ctttttcttt
     1141 aaaaccgaaa agattacttc gcgttatgca ggcttcctcg ctcactgact cgctgcgctc
     1201 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     1261 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     1321 ccgtaaaaag gccgcgttgc tggcgttttt ccacaggctc cgcccccctg acgagcatca
     1381 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     1441 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     1501 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     1561 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     1621 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     1681 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     1741 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     1801 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     1861 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     1921 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     1981 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     2041 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     2101 tgacagctcg aggcttggat tctcaccaat aaaaaacgcc cggcggcaac cgagcgttct
     2161 gaacaaatcc agatggagtt ctgaggtcat tactggatct atcaa
//
