LOCUS       sequence-50263-e         881 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 881)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: PDLIM5
FEATURES             Location/Qualifiers
     source          1..881
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             43..60
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             85..105
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             432..449
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(502..520)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      516..563
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(687..706)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ctctgaatat tttgtttact ttaagaagga gatatacata tgcaccatca tcatcatcat
       61 tcttctggtg tagatctggg taccgagaac ctgtacttcc aatccatgag caactacagt
      121 gtgtcactgg ttggcccagc tccttggggt ttccggctgc agggcggtaa ggatttcaac
      181 atgcctctga caatctctag tctaaaagat ggcggcaagg cagcccaggc aaatgtaaga
      241 ataggcgatg tggttctcag cattgatgga ataaatgcac aaggaatgac tcatcttgaa
      301 gcccagaata agattaaggg ttgtacaggc tctttgaata tgactctgca aagagaatct
      361 gacttgtgac agtaaaggtg gatacggatc cgaattcgag ctccgtcgac aagcttgcgg
      421 ccgcactcga gcaccaccac caccaccact gagatccggc tgctaacaaa gcccgaaagg
      481 aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta
      541 aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggattg gcgaatggga
      601 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
      661 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
      721 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
      781 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
      841 atcgccctga tagacggttt ttcgcccttt gacgttggag t
//
