LOCUS       sequence-50264-e         893 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 893)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: PDLIM5
FEATURES             Location/Qualifiers
     source          1..893
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(17..34)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(361..381)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(406..423)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             434..456
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    471..495
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(495..514)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(496..514)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(598..617)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 tagcagccgg atctcagtgg tggtggtggt ggtgctcgag tgcggccgca agcttgtcga
       61 cggagctcga attcggatcc gtatccacct ttactgtcac aagtcagatt ctctttgcag
      121 agtcatattc aaagagcctg tacaaccctt aatcttattc tgggcttcaa gatgagtcat
      181 tccttgtgca tttattccat caatgctgag aaccacatcg cctattctta catttgcctg
      241 ggctgccttg ccgccatctt ttagactaga gattgtcaga ggcatgttga aatccttacc
      301 gccctgcagc cggaaacccc aaggagctgg gccaaccagt gacacactgt agttgctcat
      361 ggattggaag tacaggttct cggtacccag atctacacca gaagaatgat gatgatgatg
      421 gtgcatatgt atatctcctt cttaaagtta aacaaaatta tttctagagg ggaattgtta
      481 tccgctcaca attcccctat agtgagtcgt attaatttcg cgggatcgag atctcgatcc
      541 tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt gctggcgcct
      601 atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc atgagcgctt
      661 gtttcggcgt gggtatggtg gcaggccccg tggccggggg actgttgggc gccatctcct
      721 tgcatgcacc attccttgcg gcggcggtgc tcaacggcct caacctacta ctgggctgct
      781 tcctaatgca ggagtcgcat aagggagagc gtcgagatcc cggacaccat cgaatggcgc
      841 aaaacctttc gcggtatggc atgatagcgc ccggaaagag agtcaattca ggg
//
