LOCUS       sequence-502694-        8829 bp    DNA     circular SYN 11-MAR-2026
DEFINITION  Expresses REPAIR-mini (V2) in insect cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8829)
  AUTHORS   Ishikawa J, Kato K, Kannan S, Okazaki S, Ishiguro S, Yamashita K, 
            Yachie N, Nishizawa T, Zhang F, Nishimasu H
  TITLE     Structural insights into RNA-guided RNA editing by the Cas13b-ADAR2 
            complex.
  JOURNAL   Nat Struct Mol Biol. 2025 Aug;32(8):1567-1578. doi: 
            10.1038/s41594-025-01529-1. Epub 2025 Apr 11.
  PUBMED    40217120
REFERENCE   2  (bases 1 to 8829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 11, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41594-025-01529-1"; journalName: "Nat Struct Mol Biol"; 
            date: "2025-08"; volume: "32"; issue: "8"; pages: "1567-1578"
COMMENT     Sequence Label: pFastBac-V2-REPAIR-mini
FEATURES             Location/Qualifiers
     source          1..8829
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(join(110..115,116..132,133..138))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        490..581
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     506..523
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             648..665
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             678..701
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             714..737
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             3579..3605
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     polyA_signal    4905..5039
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4942..4961)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4996..5015
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  5068..5233
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      5417..5871
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5504..5523)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5713..5734
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5897..6001
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6002..6070,6071..6862)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6220..6239)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7033..7621
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7522..7541
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7775..7792
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  7926..8150
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(8217..8750)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
ORIGIN
        1 aaaaaaacag tcataacaag ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc
       61 ggtcaaggtt ctggaccagt tgcgtgagcg catacgctac ttgcattaca gtttacgaac
      121 cgaacaggct tatgtcaact gggttcgtgc cttcatccgt ttccacggtg tgcgtcaccc
      181 ggcaaccttg ggcagcagcg aagtcgaggc atttctgtcc tggctggcga acgagcgcaa
      241 ggtttcggtc tccacgcatc gtcaggcatt ggcggccttg ctgttcttct acggcaaggt
      301 gctgtgcacg gatctgccct ggcttcagga gatcggaaga cctcggccgt cgcggcgctt
      361 gccggtggtg ctgaccccgg atgaagtggt tcgcatcctc ggttttctgg aaggcgagca
      421 tcgtttgttc gcccaggact ctagctatag ttctagtggt tggctacgta tactccggaa
      481 tattaataga tcatggagat aattaaaatg ataaccatct cgcaaataaa taagtatttt
      541 actgttttcg taacagtttt gtaataaaaa aacctataaa tattccggat tattcatacc
      601 gtcccaccat cgggcgcgga tctcggtccg aaaccatgtc gtactaccat caccatcacc
      661 atcacgatgg tgctagctgg agccacccgc agttcgaaaa aggatctacg acccttgaag
      721 tcctctttca gggacccatg aacatccccg ctctggtgga aaaccagaag aagtactttg
      781 gcacctacag cgtgatggcc atgctgaacg ctcagaccgt gctggaccac atccagaagg
      841 tggccgatat tgagggcgag cagaacgaga acaacgagaa tctgtggttt caccccgtga
      901 tgagccacct gtacaacgcc aagaacggct acgacaagca gcccgagaaa accatgttca
      961 tcatcgagcg gctgcagagc tacttcccat tcctgaagat catggccgag aaccagagag
     1021 agtacagcaa cggcaagtac aagcagaacc gcgtggaagt gaacagcaac gacatcttcg
     1081 aggtgctgaa gcgcgccttc ggcgtgctga agatgtacag ggacctgacc aacgcataca
     1141 agacctacga ggaaaagctg aacgacggct gcgagttcct gaccagcaca gagcaacctc
     1201 tgagcggcat gatcaacaac tactacacag tggccctgcg gaacatgaac gagagatacg
     1261 gctacaagac agaggacctg gccttcatcc aggacaagcg gttcaagttc gtgaaggacg
     1321 cctacggcaa gaaaaagtcc caagtgaata ccggattctt cctgagcctg caggactaca
     1381 acggcgacac acagaagaag ctgcacctga gcggagtggg aatcgccctg ctgatctgcc
     1441 tgttcctgga caagcagtac atcaacatct ttctgagcag gctgcccatc ttctccagct
     1501 acaatgccca gagcgaggaa cggcggatca tcatcagatc cttcggcatc aacagcatca
     1561 agctgcccaa ggaccggatc cacagcgaga agtccaacaa gagcgtggcc atggatatgc
     1621 tcaacgaagt gaagcggtgc cccgacgagc tgttcacaac actgtctgcc gagaagcagt
     1681 cccggttcag aatcatcagc gacgaccaca atgaagtgct gatgaagcgg agcagcgaca
     1741 gattcgtgcc tctgctgctg cagtatatcg attacggcaa gctgttcgac cacatcaggt
     1801 tccacgtgaa catgggcaag ctgagatacc tgctgaaggc cgacaagacc tgcatcgacg
     1861 gccagaccag agtcagagtg atcgagcagc ccctgaacgg cttcggcaga ctggaagagg
     1921 ccgagacaat gcggaagcaa gagaacggca ccttcggcaa cagcggcatc cggatcagag
     1981 acttcgagaa catgaagcgg gacgacgcca atcctgccaa ctatccctac atcgtggaca
     2041 cctacacaca ctacatcctg gaaaacaaca aggtcgagat gtttatcaac gacaaagagg
     2101 acagcgcccc actgctgccc gtgatcgagg atgatagata cgtggtcaag acaatcccca
     2161 gctgccggat gagcaccctg gaaattccag ccatggcctt ccacatgttt ctgttcggca
     2221 gcaagaaaac cgagaagctg atcgtggacg tgcacaaccg gtacaagaga ctgttccagg
     2281 ccatgcagaa agaagaagtg accgccgaga atatcgccag cttcggaatc gccgagagcg
     2341 acctgcctca gaagatcctg gatctgatca gcggcaatgc ccacggcaag gatgtggacg
     2401 ccttcatcag actgaccgtg gacgacatgc tgaccgacac cgagcggaga atcaagagat
     2461 tcaaggacga ccggaagtcc attcggagcg ccgacaacaa gatgggaaag agaggcttca
     2521 agcagatctc cacaggcaag ctggccgact tcctggccaa ggacatcgtg ctgtttcagc
     2581 ccagcgtgaa cgatggcgag aacaagatca ccggcctgaa ctaccggatc atgcagagcg
     2641 ccattgccgt gtacgatagc ggcgacgatt acgaggccaa gcagcagttc aagctgatgt
     2701 tcgagaaggc ccggctgatc ggcaagggca caacagagcc tcatccattt ctgtacaagg
     2761 tgttcgcccg cagcatcccc gccaatgccg tcgagttcta cgagcgctac ctgatcgagc
     2821 ggaagttcta cctgaccggc ctgtccaacg agatcaagaa aggcaacaga gtggatgtgc
     2881 ccttcatccg gcgggaccag aacaagtgga aaacacccgc catgaagacc ctgggcagaa
     2941 tctacagcga ggatctgccc gtggaactgc ccagacagat gttcgacaat gagatcaagt
     3001 cccacctgaa gtccctgcca cagatggaag gcatcgactt caacaatgcc aacgtgacct
     3061 atctgatcgc cgagtacatg aagagagtgc tggacgacga cttccagacc ttctaccagt
     3121 ggaaccgcaa ctaccggtac atggacatgc ttaagggcga gtacgacaga aagggctccc
     3181 tgcagcactg cttcaccagc gtggaagaga gagaaggcct ctggaaagag cgggcctcca
     3241 gaacagagcg gtacagaaag caggccagca acaagatccg cagcaaccgg cagatgagaa
     3301 acgccagcag cgaagagatc gagacaatcc tggataagcg gctgagcaac agccggaacg
     3361 agtaccagaa aagcgagaaa gtgatccggc gctacagagt gcaggatgcc ctgctgtttc
     3421 tgctggccaa aaagaccctg accgaactgg ccgatttcga cggcgagagg ttcaaactga
     3481 aagaaatcat gcccgacgcc gagaagggaa tcctgagcga gggcggcggc ggatctggcg
     3541 gaggcggaag cggcggcggc ggcagcggaa gcctgcagct gcctccactt gaaagactga
     3601 cactgggatc ccagctgcat ttaccgcagg ttttagctga cgctgtctca cgcctggtcc
     3661 tgggtaagtt tggtgacctg accgacaact tctcctcccc tcacgctcgc agaaaagtgc
     3721 tggctggagt cgtcatgaca acaggcacag atgttaaaga tgccaaggtg ataagtgttt
     3781 ctacaggaac aaaatgtatt aatggtgaat acatgagtga tcgtggcctt gcattaaatg
     3841 actgccatgc agaaataata tctcggagat ccttgctcag atttctttat acacaacttg
     3901 agctttactt aaataacaaa gatgatcaaa aaagatccat ctttcagaaa tcagagcgag
     3961 gggggtttag gctgaaggag aatgtccagt ttcatctgta catcagcacc tctccctgtg
     4021 gagatgccag aatcttctca ccacatgagc caatcctgga agaaccagca gatagacacc
     4081 caaatcgtaa agcaagagga cagctacgga ccaaaataga gtctggtcag gggacgattc
     4141 cagtgcgctc caatgcgagc atccaaacgt gggacggggt gctgcaaggg gagcggctgc
     4201 tcaccatgtc ctgcagtgac aagattgcac gctggaacgt ggtgggcatc cagggatcac
     4261 tgctcagcat tttcgtggag cccatttact tctcgagcat catcctgggc agcctttacc
     4321 acggggacca cctttccagg gccatgtacc agcggatctc caacatagag gacctgccac
     4381 ctctctacac cctcaacaag cctttgctca gtggcatcag caatgcagaa gcacggcagc
     4441 cagggaaggc ccccaacttc agtgtcaact ggacggtagg cgactccgct attgaggtca
     4501 tcaacgccac gactgggaag gatgagctgg gccgcgcgtc ccgcctgtgt aagcacgcgt
     4561 tgtactgtcg ctggatgcgt gtgcacggca aggttccctc ccacttacta cgctccaaga
     4621 ttaccaagcc caacgtgtac catgagtcca agctggcggc aaaggagtac caggccgcca
     4681 aggcgcgtct gttcacagcc ttcatcaagg cggggctggg ggcctgggtg gagaagccca
     4741 ccgagcagga ccagttctca ctcacgtaag gtaccaagct tgtcgagaag tactagagga
     4801 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc
     4861 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag
     4921 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     4981 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatct
     5041 gatcactgct tgagcctagg agatccgaac cagataagtg aaatctagtt ccaaactatt
     5101 ttgtcatttt taattttcgt attagcttac gacgctacac ccagttccca tctattttgt
     5161 cactcttccc taaataatcc ttaaaaactc catttccacc cctcccagtt cccaactatt
     5221 ttgtccgccc acagcggggc atttttcttc ctgttatgtt tttaatcaaa catcctgcca
     5281 actccatgtg acaaaccgtc atcttcggct actttttctc tgtcacagaa tgaaaatttt
     5341 tctgtcatct cttcgttatt aatgtttgta attgactgaa tatcaacgct tatttgcagc
     5401 ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     5461 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     5521 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     5581 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     5641 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     5701 acgttcttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     5761 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     5821 tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac
     5881 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     5941 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     6001 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     6061 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     6121 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     6181 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     6241 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     6301 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     6361 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     6421 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     6481 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     6541 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     6601 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     6661 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     6721 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     6781 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     6841 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     6901 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     6961 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     7021 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     7081 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     7141 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     7201 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     7261 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     7321 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     7381 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     7441 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     7501 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     7561 ccacctctga cttgagcgtc gatttttgtg atgctcgtcg ggggggcgga gcctatggaa
     7621 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     7681 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     7741 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     7801 agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcataga
     7861 ccagccgcgt aacctggcaa aatcggttac ggttgagtaa taaatggatg ccctgcgtaa
     7921 gcgggtgtgg gcggacaata aagtcttaaa ctgaacaaaa tagatctaaa ctatgacaat
     7981 aaagtcttaa actagacaga atagttgtaa actgaaatca gtccagttat gctgtgaaaa
     8041 agcatactgg acttttgtta tggctaaagc aaactcttca ttttctgaag tgcaaattgc
     8101 ccgtcgtatt aaagaggggc gtggccaagg gcatggtaaa gactatattc gcggcgttgt
     8161 gacaatttac cgaacaactc cgcggccggg aagccgatct cggcttgaac gaattgttag
     8221 gtggcggtac ttgggtcgat atcaaagtgc atcacttctt cccgtatgcc caactttgta
     8281 tagagagcca ctgcgggatc gtcaccgtaa tctgcttgca cgtagatcac ataagcacca
     8341 agcgcgttgg cctcatgctt gaggagattg atgagcgcgg tggcaatgcc ctgcctccgg
     8401 tgctcgccgg agactgcgag atcatagata tagatctcac tacgcggctg ctcaaacttg
     8461 ggcagaacgt aagccgcgag agcgccaaca accgcttctt ggtcgaaggc agcaagcgcg
     8521 atgaatgtct tactacggag caagttcccg aggtaatcgg agtccggctg atgttgggag
     8581 taggtggcta cgtctccgaa ctcacgaccg aaaagatcaa gagcagcccg catggatttg
     8641 acttggtcag ggccgagcct acatgtgcga atgatgccca tacttgagcc acctaacttt
     8701 gttttagggc gactgccctg ctgcgtaaca tcgttgctgc tgcgtaacat cgttgctgct
     8761 ccataacatc aaacatcgac ccacggcgta acgcgcttgc tgcttggatg cccgaggcat
     8821 agactgtac
//
