LOCUS       sequence-502695-       14739 bp    DNA     circular SYN 11-MAR-2026
DEFINITION  Expresses gRNA and VP64-dSpCas9-VP64-2A-Thy1.1 from lentiviral 
            vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14739)
  AUTHORS   Reisman SJ, Halabi D, Miller SE, Song L, Geraghty S, Sangvai N, Rice
            G, Safi A, Crawford GE, Gersbach CA
  TITLE     Comprehensive profiling of transcription factors for reprogramming 
            human astrocytes to neuronal cells through endogenous CRISPR-based 
            gene activation
  JOURNAL   bioRxiv 2025.10.11.681828
REFERENCE   2  (bases 1 to 14739)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 11, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.10.11.681828"
COMMENT     Sequence Label: pLV hU6-gRNA hUbC-dSpCas9-2xVP64-2A-Thy1.1
FEATURES             Location/Qualifiers
     source          1..14739
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(223..243)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(250..396)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDM"
     CDS             complement(412..477)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     regulatory      477..486
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(515..1726)
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     complement(659..678)
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     promoter        1755..2003
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     1755..1775
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     1926..1945
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    2157..2274
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(2572..2616)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2801..3034
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    3527..3652
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             3699..3879
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(3897..4095)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(3922..3942)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        4097..4476
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     4648..4667
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        4742..4846
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4847..4915,4916..5707)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5065..5084)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5878..6466
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6367..6386
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6620..6637
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6754..6775
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6790..6795,6796..6813,6814..6820)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6828..6844
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6833..6855
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6852..6868
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6852..6868
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    6917..7038
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6928..6947)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6982..7001
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(7168..7542)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(7561..7608)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(7656..7985)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7670..7805
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7724..7743)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     7970..7990
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(7999..8427)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8109..8130)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8321..8340
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    8473..8697
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     8686..8703
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             8729..8909
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     9414..9430
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     9415..9431
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    9434..10022
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             9540..9551
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     9949..9969
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     protein_bind    10047..10080
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(10096..10584)
                     /codon_start=1
                     /product="mouse GPI-anchored cell surface glycoprotein of 
                     the immunoglobulin superfamily"
                     /label=Thy1.1
                     /note="This allele of Thy1 is also known as CD90.1."
                     /translation="MNPAISVALLLSVLQVSRGQKVTSLTACLVNQNLRLDCRHENNTK
                     DNSIQHEFSLTREKRKHVLSGTLGIPEHTYRSRVTLSNQPYIKVLTLANFTTKDEGDYF
                     CELRVSGANPMSSNKSISVYRDKLVKCGGISLLVQNTSWMLLLLLSLSLLQALDFISL"
     CDS             complement(10591..10644)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(10654..10803)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             complement(10816..10836)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(join(10849..12363,12364..12366,12367..12432,
                     12433..12438,12439..14739,1..183,184..186,187..213))
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=Cas9m4
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the mutations D10A, D839A, 
                     H840A, and N863A (Mali et al., 2013)"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(join(10849..12432,12433..12435,12436..14739,
                     1..183,184..186,187..213))
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(join(10849..14739,1..213))
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
ORIGIN
        1 tctgcgccgt gctgttcttt tgagccgcgt ggcttcggcg gtttccccgg agtcgaacag
       61 gagggcgcca atgaggttct tctttatgct gtggcgatcg gtattgccca gaactttgaa
      121 ttttttgctc ggcaccttgt actcgtccgt aatgacggcc cagccgacgc tgtttgtgcc
      181 gatggcgagc ccaatggagt acttcttgtc cattccgcgg cccaccttcc tcttcttctt
      241 ggggttaacc atgtccaggt cgaaatcatc aagggcgtca ctgccgagca tgtcgaggtc
      301 aaagtcatca agggcatccg aaccaagcat gtcaaggtca aaatcatcga gggcgtcact
      361 tcccagcata tccagatcaa aatcgtccaa tgcgtcagcc cgcggaccgt gcttgtcatc
      421 gtcatccttg taatcgatgt catgatcttt ataatcaccg tcatggtctt tgtagtccat
      481 ggtggctcta gagtcgacct gcagcccaag cttcgtctaa caaaaaagcc aaaaacggcc
      541 agaatttagc ggacaattta ctagtctaac actgaaaatt acatattgac ccaaatgatt
      601 acatttcaaa aggtgcctaa aaaacttcac aaaacacact cgccaacccc gagcgcatag
      661 ttcaaaaccg gagcttcagc tacttaagaa gataggtaca taaaaccgac caaagaaact
      721 gacgcctcac ttatccctcc cctcaccaga ggtccggcgc ctgtcgattc aggagagcct
      781 accctaggcc cgaaccctgc gtcctgcgac ggagaaaagc ctaccgcaca cctaccggca
      841 ggtggcccca ccctgcatta taagccaaca gaacgggtga cgtcacgaca cgacgagggc
      901 gcgcgctccc aaaggtacgg gtgcactgcc caacggcacc gccataactg ccgcccccgc
      961 aacagacgac aaaccgagtt ctccagtcag tgacaaactt cacgtcaggg tccccagatg
     1021 gtgccccagc ccatctcacc cgaataagag ctttcccgca ttagcgaagg cctcaagacc
     1081 ttgggttctt gccgcccacc atgcccccca ccttgtttca acgacctcac agcccgcctc
     1141 acaagcgtct tccattcaag actcgggaac agccgccatt ttgctgcgct ccccccaacc
     1201 cccagttcag ggcaaccttg ctcgcggacc cagactacag cccttggcgg tctctccaca
     1261 cgcttccgtc ccaccgagcg gcccggcggc cacgaaagcc ccggccagcc cagcagcccg
     1321 ctactcacca agtgacgatc acagcgatcc acaaacaaga accgcgaccc aaatcccggc
     1381 tgcgacggaa ctagctgtgc cacacccggc gcgtccttat ataatcatcg gcgttcaccg
     1441 ccccacggag atccctccgc agaatcgccg agaagggact acttttcctc gcctgttccg
     1501 ctctctggaa agaaaaccag tgccctagag tcacccaagt cccgtcctaa aatgtccttc
     1561 tgctgatact ggggttctaa ggccgagtct tatgagcagc gggccgctgt cctgagcgtc
     1621 cgggcggaag gatcaggacg ctcgctgcgc ccttcgtctg acgtggcagc gctcgccgtg
     1681 aggagggggg cgcccgcggg aggcgccaaa acccggcgcg gaggccagat ctggagccga
     1741 cacgggttaa ttaagagggc ctatttccca tgattccttc atatttgcat atacgataca
     1801 aggctgttag agagataatt ggaattaatt tgactgtaaa cacaaagata ttagtacaaa
     1861 atacgtgacg tagaaagtaa taatttcttg ggtagtttgc agttttaaaa ttatgtttta
     1921 aaatggacta tcatatgctt accgtaactt gaaagtattt cgatttcttg gctttatata
     1981 tcttgtggaa aggacgaaac accggagacg gacgtctctg tttaagagct atgctggaaa
     2041 cagcatagca agtttaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc
     2101 ggtgcttttt ttttaattaa ccaaactgga tctctgctgt ccctgtaata aacccgaaaa
     2161 ttttgaattt ttgtaatttg tttttgtaat tctttagttt gtatgtctgt tgctattatg
     2221 tctactattc tttcccctgc actgtacccc ccaatccccc cttttctttt aaaattgtgg
     2281 atgaatactg ccatttgtct gcagaattgg cgcacgcagt gccgatccgt tcactaatcg
     2341 aatggatctg tctctgtctc tctctccacc ttcttcttct attccttcgg gcctgtcggg
     2401 tcccctcggg gttgggaggt gggtctgaaa cgataatggt gaatatccct gcctaactct
     2461 attcactata gaaagtacag caaaaactat tcttaaacct accaagcctc ctactatcat
     2521 tatgaataat tttatatacc acagccaatt tgttatgtta aaccaattcc acaaacttgc
     2581 ccatttatct aattccaata attcttgttc attcttttct tgctggtttt gcgattcttc
     2641 aattaaggag tgtattaagc ttgtgtaatt gttaatttct ctgtcccact ccatccaggt
     2701 cgtgtgattc caaatctgtt ccagagattt attactccaa ctagcattcc aaggcacagc
     2761 agtggtgcaa atgagttttc cagagcaacc ccaaatcccc aggagctgtt gatcctttag
     2821 gtatctttcc acagccagga ttcttgcctg gagctgcttg atgccccaga ctgtgagttg
     2881 caacagatgc tgttgcgcct caatagccct cagcaaattg ttctgctgct gcactatacc
     2941 agacaataat tgtctggcct gtaccgtcag cgtcattgac gctgcgccca tagtgcttcc
     3001 tgctgctccc aagaacccaa ggaacaaagc tcctattccc actgctcttt tttctctctg
     3061 caccactctt ctctttgcct tggtgggtgc tactcctaat ggttcaattt ttactacttt
     3121 atatttatat aattcacttc tccaattgtc cctcatatct cctcctccag gtctgaagat
     3181 cagcggccgc ttgctgtgcg gtggtcttac ttttgttttg ctcttcctct atcttgtcta
     3241 aagcttcctt ggtgtctttt atctctatcc tttgatgcac acaatagagg gttgctactg
     3301 tattatataa tgatctaagt tcttctgatc ctgtctgaag ggatggttgt agctgtccca
     3361 gtatttgtct acagccttct gatgtttcta acaggccagg attaactgcg aatcgttcta
     3421 gctccctgct tgcccatact atatgtttta atttatattt tttctttccc cctggcctta
     3481 accgaatttt ttcccatcgc gatctaattc tcccccgctt aatactgacg ctctcgcacc
     3541 catctctctc cttctagcct ccgctagtca aaatttttgg cgtactcacc agtcgccgcc
     3601 cctcgcctct tgccgtgcgc gcttcagcaa gccgagtcct gcgtcgagag agctcctctg
     3661 gtttcccttt cgctttcaag tccctgttcg ggcgccactg ctagagattt tccacactga
     3721 ctaaaagggt ctgagggatc tctagttacc agagtcacac aacagacggg cacacactac
     3781 ttgaagcact caaggcaagc tttattgagg cttaagcagt gggttcccta gttagccaga
     3841 gagctcccag gctcagatct ggtctaacca gagagaccca gtacaggcaa aacgcgctgc
     3901 ttatatagac ctcccaccgt acacgcctac cgcccatttg cgtcaatggg gcggagttgt
     3961 tacgacattt tggaaagtcc cgttgatttt ggtgccaaaa caaactccca ttgacgtcaa
     4021 tggggtggag acttggaaat ccccgtgagt caaaccgcta tccacgccca ttgatgtact
     4081 gccaaaaccg catcaccatg gtaatagcga tgactaatac gtagatgtac tgccaagtag
     4141 gaaagtccca taaggtcatg tactgggcat aatgccaggc gggccattta ccgtcattga
     4201 cgtcaatagg gggcgtactt ggcatatgat acacttgatg tactgccaag tgggcagttt
     4261 accgtaaata ctccacccat tgacgtcaat ggaaagtccc tattggcgtt actatgggaa
     4321 catacgtcat tattgacgtc aatgggcggg ggtcgttggg cggtcagcca ggcgggccat
     4381 ttaccgtaag ttatgtaacg cggaactcca tatatgggct atgaactaat gaccccgtaa
     4441 ttgattacta ttaataacta gtcaataatc aatgtcaacg cgtatatctg gcccgtacat
     4501 cgcgaagcag cgcaaaacgc ctaaccctaa gcagattctt catgcaattg tcggtcaagc
     4561 cttgccttgt tgtagcttaa attttgctcg cgcactactc agcgacctcc aacacacaag
     4621 cagggagcag atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc
     4681 ataggggatc gggagatctc ccgatccgtc gacgtcaggt ggcacttttc ggggaaatgt
     4741 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     4801 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     4861 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     4921 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     4981 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     5041 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     5101 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     5161 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     5221 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     5281 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     5341 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     5401 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     5461 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     5521 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     5581 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     5641 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     5701 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     5761 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     5821 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     5881 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     5941 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     6001 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     6061 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     6121 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     6181 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     6241 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     6301 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     6361 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     6421 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     6481 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     6541 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     6601 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     6661 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     6721 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     6781 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     6841 acaatttcac acaggaaaca gctatgacca tgattacgcc aagctctagc tagaggtcga
     6901 cggtatacag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag
     6961 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
     7021 agctgcaata aacaagttgg ggtgggcgaa gaactccagc atgagatccc cgcgctggag
     7081 gatcatccag ccggcgtccc ggaaaacgat tccgaagccc aacctttcat agaaggcggc
     7141 ggtggaatcg aaatctcgta gcacgtgtca gtcctgctcc tcggccacga agtgcacgca
     7201 gttgccggcc gggtcgcgca gggcgaactc ccgcccccac ggctgctcgc cgatctcggt
     7261 catggccggc ccggaggcgt cccggaagtt cgtggacacg acctccgacc actcggcgta
     7321 cagctcgtcc aggccgcgca cccacaccca ggccagggtg ttgtccggca ccacctggtc
     7381 ctggaccgcg ctgatgaaca gggtcacgtc gtcccggacc acaccggcga agtcgtcctc
     7441 cacgaagtcc cgggagaacc cgagccggtc ggtccagaac tcgaccgctc cggcgacgtc
     7501 gcgcgcggtg agcaccggaa cggcactggt caacttggcc atggtttagt tcctcacctt
     7561 gtcgtattat actatgccga tatactatgc cgatgattaa ttgtcaacac gtgctgatca
     7621 gatccgaaaa tggatataca agctcccggg agctttttgc aaaagcctag gcctccaaaa
     7681 aagcctcctc actacttctg gaatagctca gaggcagagg cggcctcggc ctctgcataa
     7741 ataaaaaaaa ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc
     7801 gggatgggcg gagttagggg cgggactatg gttgctgact aattgagatg catgctttgc
     7861 atacttctgc ctgctgggga gcctggggac tttccacacc tggttgctga ctaattgaga
     7921 tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca ccctaactga
     7981 cacacattcc acagaattaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
     8041 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
     8101 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
     8161 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt
     8221 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
     8281 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
     8341 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
     8401 cgcttaatgc gccgctacag ggcgcgtggg gataccccct agagccccag ctggttcttt
     8461 ccgcctcaga agccatagag cccaccgcat ccccagcatg cctgctattg tcttcccaat
     8521 cctccccctt gctgtcctgc cccaccccac cccccagaat agaatgacac ctactcagac
     8581 aatgcgatgc aatttcctca ttttattagg aaaggacagt gggagtggca ccttccaggg
     8641 tcaaggaagg cacgggggag gggcaaacaa cagatggctg gcaactagaa ggcacagtcg
     8701 aggctgatca gcgggtttaa acgggccctg ctagagattt tccacactga ctaaaagggt
     8761 ctgagggatc tctagttacc agagtcacac aacagacggg cacacactac ttgaagcact
     8821 caaggcaagc tttattgagg cttaagcagt gggttcccta gttagccaga gagctcccag
     8881 gctcagatct ggtctaacca gagagaccca gtacagtccg gatgcagctc tcgggccatg
     8941 tgatgaaatg ctaggcggct gtcaaacctc cactctaata cttctctctc cgggtcatcc
     9001 atcccatgca ggctcacagg gtgtaacaag cgggtgttct ctccttcatt ggcttcttct
     9061 accttctctt gctcaactgg tactagcttg tagcaccatc caaaggtcag tggatatctg
     9121 atccctggcc ctggtgtgta gttctgccaa tcagggaagt agccttgtgt gtggtagatc
     9181 cacagatcaa ggatatcttg tcttcgttgg gagtgaatta gcccttccag tccccccttt
     9241 tcttttaaaa agtggctaag atctacagct gccttgtaag tcattggtct taaaggtacc
     9301 tgaggtgtga ctggaaaacc cacctcctcc tcctcttgtg cttctagcca ggcacaatca
     9361 gcattggtag ctgctgtatt gctacttgtg attgctccat gtttttctag gtctcgaggt
     9421 cgacggtatc gatgcgggga ggcggcccaa agggagatcc gactcgtctg agggcgaagg
     9481 cgaagacgcg gaagaggccg cagagccggc agcaggccgc gggaaggaag gtccgctgga
     9541 ttgagggccg aagggacgta gcagaaggac gtcccgcgca gaatccaggt ggcaacacag
     9601 gcgagcagcc aaggaaagga cgatgatttc cccgacaaca ccacggaatt gtcagtgccc
     9661 aacagccgag cccctgtcca gcagcgggca aggcaggcgg cgatgagttc cgccgtggca
     9721 atagggaggg ggaaagcgaa agtcccggaa aggagctgac aggtggtggc aatgccccaa
     9781 ccagtggggg ttgcgtcagc aaacacagtg cacaccacgc cacgttgcct gacaacgggc
     9841 cacaactcct cataaagaga cagcaaccag gatttataca aggaggagaa aatgaaagcc
     9901 atacgggaag caatagcatg atacaaaggc attaaagcag cgtatccaca tagcgtaaaa
     9961 ggagcaacat agttaagaat accagtcaat ctttcacaaa ttttgtaatc cagaggttga
    10021 ttatcgataa gcttgatatc gaattcataa cttcgtataa tgtatgctat acgaagttat
    10081 ctattatcaa ccggttcaca gagaaatgaa gtccagggct tggaggaggg agagggaaag
    10141 cagcagcagc agcatccagg atgtgttctg aaccagcagg cttatgccgc cacacttgac
    10201 cagcttgtct ctatacacac tgatactttt attggagctc atgggattcg cgcccgaaac
    10261 gcgaagctca caaaagtagt cgccctcatc cttggtggtg aagttggcta gggtaaggac
    10321 cttgatatag ggctggttgg agagggtgac gcgggagcgg tacgtgtgct cgggtatccc
    10381 aagggtgcct gagagcacgt gcttcctctt ctctcgggtc aggctgaact catgctggat
    10441 ggagttatcc ttggtgttat tctcatggcg gcagtccagg cgaaggtttt ggttcaccag
    10501 gcaggctgtc aggctggtca ccttctgccc tcgggacacc tgcaagactg agagcaggag
    10561 agcgacgctg atggctgggt tcatggtacc agggccggga ttctcctcca cgtcaccgca
    10621 tgttagaaga cttcctctgc cctcgctagc gattaacata tcgagatcga aatcgtccag
    10681 agcatcggag ccgagcatgt ccagatcaaa gtcatccaat gcgtcgcttc ccaacatatc
    10741 caggtcaaag tcatcgaggg catcagaacc cagcatgtcg agatcgaaat cgtccagcgc
    10801 gtcggcgcgg ctagccacct tcctcttctt cttggggtca gccctgctgt ctccaccgag
    10861 ctgagagagg tcgattcttg tttcatagag ccccgtaatt gactgatgaa tcagtgtggc
    10921 gtccaggacc tcctttgtag aggtgtaccg ctttctgtct atggtggtgt cgaagtactt
    10981 gaaggctgca ggcgcgccca agttggtcag agtaaacaag tggataatgt tttctgcctg
    11041 ctccctgatg ggcttatccc tgtgcttatt gtaagcagaa agcaccttat cgaggttagc
    11101 gtcggcgagg atcactcttt tggagaattc gcttatttgc tcgatgatct catcaaggta
    11161 gtgtttgtgt tgttccacga acagctgctt ctgctcatta tcttcgggag accctttgag
    11221 cttttcatag tggctggcca gatacaagaa attaacgtat ttagagggca gtgccagctc
    11281 gttacctttc tgcagctcgc ccgcactagc gagcattcgt ttccggccgt tttcaagctc
    11341 aaagagagag tacttgggaa gcttaatgat gaggtctttt ttgacctctt tatatccttt
    11401 cgcctcgaga aagtcgatgg ggtttttttc gaagcttgat cgctccatga ttgtgatgcc
    11461 cagcagttcc ttgacgcttt tgagtttttt agacttccct ttctccactt tggccacaac
    11521 cagtacactg taagcgactg taggagaatc gaatccgccg tatttcttgg ggtcccaatc
    11581 ttttttgcgt gcgatcagct tgtcgctgtt ccttttcggg aggatacttt ccttggagaa
    11641 gcctccggtc tgtacttcgg tctttttaac gatgttcacc tgcggcatgg acaggacctt
    11701 ccggactgtc gcgaaatccc tacccttgtc ccacacgatt tctcctgttt ctccgtttgt
    11761 ttcgataagt ggtcgcttcc gaatctctcc attggccagt gtaatctcgg tcttgaaaaa
    11821 attcataata ttgctgtaaa agaagtactt agcggtggcc ttgcctattt cctgctcaga
    11881 ctttgcgatc attttcctaa catcgtacac tttatagtct ccgtaaacaa attcagattc
    11941 aagcttggga tattttttga taagtgcagt gcctaccact gcattcaggt aggcatcatg
    12001 cgcatggtgg taattgttga tctctctcac cttataaaac tgaaagtcct ttctgaaatc
    12061 tgagaccagc ttagacttca gagtaataac tttcacctct cgaatcagtt tgtcattttc
    12121 atcgtacttg gtgttcatgc gtgaatcgag aatttgggcc acgtgcttgg tgatctggcg
    12181 tgtctcaaca agctgccttt tgatgaagcc ggctttatcc aactcagaca ggccacctcg
    12241 ttcagcctta gtcagattat cgaacttccg ttgtgtgatc agtttggcgt tcagcagctg
    12301 ccgccaataa tttttcattt tcttgacaac ttcttctgag gggacgttat cactcttccc
    12361 tctattttta tcggatcttg tcaacacttt attatcaata gaatcatctt tgagaaaaga
    12421 ctggggcacg atggcatcca cgtcgtagtc ggagagccga ttgatgtcca gttcctgatc
    12481 cacgtacatg tccctgccgt tctgcaggta gtacaggtag agcttctcat tctgaagctg
    12541 ggtgttttca actgggtgtt ccttaaggat ttgggacccc agttctttta taccctcttc
    12601 aatcctcttc atcctttccc tactgttctt ctgtcccttc tgggtagttt ggttctctcg
    12661 ggccatctcg ataacgatat tctcgggctt atgccttccc attactttga cgagttcatc
    12721 cacgacctta acggtctgca gtattccctt tttgatagct gggctacctg caagattagc
    12781 gatgtgctcg tgaagactgt ccccctggcc agaaacttgt gctttctgga tgtcctcctt
    12841 aaaggtgaga gagtcatcat ggatcaactg catgaagttc cggttggcaa atccatcgga
    12901 cttaagaaaa tccaggattg tctttccact ctgcttgtct cggatcccat tgatcagttt
    12961 tcttgacagc cgcccccatc ctgtatatcg gcgcctcttg agctgtttca tgactttgtc
    13021 gtcgaagaga tgagcgtaag ttttcaagcg ttcttcaatc atctccctat cttcaaacaa
    13081 cgtaagggtg aggacaatgt cctcaagaat gtcctcgttc tcctcattgt ccaggaagtc
    13141 cttgtcttta atgattttca ggagatcgtg atacgttccc agggatgcgt tgaagcgatc
    13201 ctccactccg ctgatttcaa cagagtcgaa acattcaatc tttttgaaat agtcttcttt
    13261 gagctgtttc acggtaactt tccggttcgt cttgaagagg aggtccacga tagctttctt
    13321 ctgctctcca gacaggaatg ctggctttct catcccttct gtgacgtatt tgaccttggt
    13381 gagctcgtta taaactgtga agtactcgta cagcagagag tgtttaggaa gcaccttttc
    13441 gttaggcaga tttttatcaa agttagtcat cctttcgatg aaggactggg cagaggcccc
    13501 cttatccacg acttcctcga agttccaggg agtgatggtc tcttctgatt tgcgagtcat
    13561 ccacgcgaat ctggaatttc cccgggcgag ggggcctaca tagtagggta tccgaaatgt
    13621 gaggattttc tcaatctttt ccctgttatc tttcaaaaag gggtagaaat cctcttgccg
    13681 cctgaggata gcgtgcagtt cgcccaggtg aatctggtgg gggatgcttc cattgtcgaa
    13741 agtgcgctgt ttgcgcaaca gatcttctct gttaagcttt accagcagct cctcggtgcc
    13801 gtccattttt tccaagatgg gcttaataaa tttgtaaaat tcctcctggc ttgctccgcc
    13861 gtcaatgtat ccggcgtagc catttttaga ctgatcgaag aaaatttcct tgtacttctc
    13921 aggcagttgc tgtctgacaa gggccttcag caaagtcaag tcttggtggt gctcatcata
    13981 gcgcttgatc atactagcgc tcagcggagc tttggtgatc tccgtgttca ctcgcagaat
    14041 atcactcagc agaatggcgt ctgacaggtt ctttgccgcc aaaaaaaggt ctgcgtactg
    14101 gtcgccgatc tgggccagca gattgtcgag atcatcatcg taggtgtctt tgctcagttg
    14161 aagcttggca tcttcggcca ggtcgaagtt agatttaaag ttgggggtca gcccgagtga
    14221 cagggcgata agattaccaa acaggccgtt cttcttctcc ccagggagct gtgcgatgag
    14281 gttttcgagc cgccgggatt tggacagcct agcgctcagg attgctttgg cgtcaactcc
    14341 ggatgcgttg atcgggttct cttcgaaaag ctgattgtaa gtctgaacca gttggataaa
    14401 gagtttgtcg acatcgctgt tgtctgggtt caggtccccc tcgatgagga agtgtccccg
    14461 aaatttgatc atatgcgcca gcgcgagata gatcaaccgc aagtcagcct tatcagtact
    14521 gtctacaagc ttcttcctca gatgatatat ggttgggtac ttttcatggt acgccacctc
    14581 gtccacgata ttgccaaaga ttgggtggcg ctcgtgcttt ttatcctcct ccaccaaaaa
    14641 ggactcctcc agcctatgga agaaagagtc atccacctta gccatctcat tactaaagat
    14701 ctcctgcagg tagcagatcc gattctttct gcgggtata
//
