LOCUS       sequence-502696-       14774 bp    DNA     circular SYN 11-MAR-2026
DEFINITION  Expresses gRNA and VP64-dSpCas9-VP64-2A-PuroR from lentiviral 
            vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14774)
  AUTHORS   Reisman SJ, Halabi D, Miller SE, Song L, Geraghty S, Sangvai N, Rice
            G, Safi A, Crawford GE, Gersbach CA
  TITLE     Comprehensive profiling of transcription factors for reprogramming 
            human astrocytes to neuronal cells through endogenous CRISPR-based 
            gene activation
  JOURNAL   bioRxiv 2025.10.11.681828
REFERENCE   2  (bases 1 to 14774)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 11, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.10.11.681828"
COMMENT     Sequence Label: pLV hU6-gRNA hUbC-dSpCas9-2xVP64-2A-PuroR
FEATURES             Location/Qualifiers
     source          1..14774
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      25..160
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     87..106
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        222..269
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             288..662
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    792..913
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(829..848)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     883..902
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(962..978)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(962..978)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(975..997)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    986..1002
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1010..1016,1017..1034,1035..1040))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1055..1076
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1193..1210)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1364..1952)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1444..1463)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2123..2914,2915..2983))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2746..2765
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2984..3088)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3163..3182)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3354..3733
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3735..3933
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3888..3908
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             3951..4131
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4178..4303
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4796..5029
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5214..5258
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5556..5673
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        complement(5827..6075)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(5885..5904)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(6055..6075)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     promoter        6104..7315
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     7152..7171
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      7344..7353
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7353..7418
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             7434..7580
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDM"
     CDS             7587..7607
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(7617..7643,7644..7646,7647..10130,10131..10136,
                     10137..10202,10203..10205,10206..11720)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=Cas9m4
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the mutations D10A, D839A, 
                     H840A, and N863A (Mali et al., 2013)"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(7617..7643,7644..7646,7647..10133,10134..10136,
                     10137..11720)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             7617..11720
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             11733..11753
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             11766..11915
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             11925..11978
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             11985..12584
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(11985..12004)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     12481..12501
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    complement(12638..12671)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    12696..13284
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(12749..12769)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(13167..13178)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(13287..13303)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             13695..13875
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(13901..13918)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    13907..14131
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      14177..14605
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(14264..14283)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     14474..14495
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(14614..14634)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        join(14619..14774,1..174)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
ORIGIN
        1 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
       61 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct
      121 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
      181 ccgggagctt gtatatccat tttcggatct gatcagcacg tgttgacaat taatcatcgg
      241 catagtatat cggcatagta taatacgaca aggtgaggaa ctaaaccatg gccaagttga
      301 ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg
      361 accggctcgg gttctcccgg gacttcgtgg aggacgactt cgccggtgtg gtccgggacg
      421 acgtgaccct gttcatcagc gcggtccagg accaggtggt gccggacaac accctggcct
      481 gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc gtgtccacga
      541 acttccggga cgcctccggg ccggccatga ccgagatcgg cgagcagccg tgggggcggg
      601 agttcgccct gcgcgacccg gccggcaact gcgtgcactt cgtggccgag gagcaggact
      661 gacacgtgct acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
      721 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
      781 tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
      841 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
      901 tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat
      961 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     1021 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     1081 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     1141 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     1201 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     1261 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     1321 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     1381 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     1441 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     1501 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     1561 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     1621 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     1681 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     1741 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     1801 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     1861 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     1921 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     1981 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     2041 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     2101 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     2161 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     2221 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     2281 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     2341 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     2401 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     2461 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     2521 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     2581 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     2641 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     2701 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     2761 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     2821 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     2881 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     2941 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     3001 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     3061 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg
     3121 acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc
     3181 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc
     3241 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct
     3301 tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg
     3361 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
     3421 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     3481 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     3541 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     3601 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     3661 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
     3721 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
     3781 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     3841 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
     3901 taggcgtgta cggtgggagg tctatataag cagcgcgttt tgcctgtact gggtctctct
     3961 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     4021 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     4081 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc
     4141 gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct
     4201 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
     4261 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
     4321 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
     4381 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
     4441 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
     4501 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
     4561 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
     4621 taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg
     4681 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     4741 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     4801 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
     4861 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     4921 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     4981 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     5041 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     5101 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca
     5161 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg
     5221 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa
     5281 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
     5341 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt
     5401 ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag
     5461 gtggagagag agacagagac agatccattc gattagtgaa cggatcggca ctgcgtgcgc
     5521 caattctgca gacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg
     5581 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
     5641 ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat
     5701 ccagtttggt taattaaaaa aaaagcaccg actcggtgcc actttttcaa gttgataacg
     5761 gactagcctt atttaaactt gctatgctgt ttccagcata gctcttaaac agagacgtcc
     5821 gtctccggtg tttcgtcctt tccacaagat atataaagcc aagaaatcga aatactttca
     5881 agttacggta agcatatgat agtccatttt aaaacataat tttaaaactg caaactaccc
     5941 aagaaattat tactttctac gtcacgtatt ttgtactaat atctttgtgt ttacagtcaa
     6001 attaattcca attatctctc taacagcctt gtatcgtata tgcaaatatg aaggaatcat
     6061 gggaaatagg ccctcttaat taacccgtgt cggctccaga tctggcctcc gcgccgggtt
     6121 ttggcgcctc ccgcgggcgc ccccctcctc acggcgagcg ctgccacgtc agacgaaggg
     6181 cgcagcgagc gtcctgatcc ttccgcccgg acgctcagga cagcggcccg ctgctcataa
     6241 gactcggcct tagaacccca gtatcagcag aaggacattt taggacggga cttgggtgac
     6301 tctagggcac tggttttctt tccagagagc ggaacaggcg aggaaaagta gtcccttctc
     6361 ggcgattctg cggagggatc tccgtggggc ggtgaacgcc gatgattata taaggacgcg
     6421 ccgggtgtgg cacagctagt tccgtcgcag ccgggatttg ggtcgcggtt cttgtttgtg
     6481 gatcgctgtg atcgtcactt ggtgagtagc gggctgctgg gctggccggg gctttcgtgg
     6541 ccgccgggcc gctcggtggg acggaagcgt gtggagagac cgccaagggc tgtagtctgg
     6601 gtccgcgagc aaggttgccc tgaactgggg gttgggggga gcgcagcaaa atggcggctg
     6661 ttcccgagtc ttgaatggaa gacgcttgtg aggcgggctg tgaggtcgtt gaaacaaggt
     6721 ggggggcatg gtgggcggca agaacccaag gtcttgaggc cttcgctaat gcgggaaagc
     6781 tcttattcgg gtgagatggg ctggggcacc atctggggac cctgacgtga agtttgtcac
     6841 tgactggaga actcggtttg tcgtctgttg cgggggcggc agttatggcg gtgccgttgg
     6901 gcagtgcacc cgtacctttg ggagcgcgcg ccctcgtcgt gtcgtgacgt cacccgttct
     6961 gttggcttat aatgcagggt ggggccacct gccggtaggt gtgcggtagg cttttctccg
     7021 tcgcaggacg cagggttcgg gcctagggta ggctctcctg aatcgacagg cgccggacct
     7081 ctggtgaggg gagggataag tgaggcgtca gtttctttgg tcggttttat gtacctatct
     7141 tcttaagtag ctgaagctcc ggttttgaac tatgcgctcg gggttggcga gtgtgttttg
     7201 tgaagttttt taggcacctt ttgaaatgta atcatttggg tcaatatgta attttcagtg
     7261 ttagactagt aaattgtccg ctaaattctg gccgtttttg gcttttttgt tagacgaagc
     7321 ttgggctgca ggtcgactct agagccacca tggactacaa agaccatgac ggtgattata
     7381 aagatcatga catcgattac aaggatgacg atgacaagca cggtccgcgg gctgacgcat
     7441 tggacgattt tgatctggat atgctgggaa gtgacgccct cgatgatttt gaccttgaca
     7501 tgcttggttc ggatgccctt gatgactttg acctcgacat gctcggcagt gacgcccttg
     7561 atgatttcga cctggacatg gttaacccca agaagaagag gaaggtgggc cgcggaatgg
     7621 acaagaagta ctccattggg ctcgccatcg gcacaaacag cgtcggctgg gccgtcatta
     7681 cggacgagta caaggtgccg agcaaaaaat tcaaagttct gggcaatacc gatcgccaca
     7741 gcataaagaa gaacctcatt ggcgccctcc tgttcgactc cggggaaacc gccgaagcca
     7801 cgcggctcaa aagaacagca cggcgcagat atacccgcag aaagaatcgg atctgctacc
     7861 tgcaggagat ctttagtaat gagatggcta aggtggatga ctctttcttc cataggctgg
     7921 aggagtcctt tttggtggag gaggataaaa agcacgagcg ccacccaatc tttggcaata
     7981 tcgtggacga ggtggcgtac catgaaaagt acccaaccat atatcatctg aggaagaagc
     8041 ttgtagacag tactgataag gctgacttgc ggttgatcta tctcgcgctg gcgcatatga
     8101 tcaaatttcg gggacacttc ctcatcgagg gggacctgaa cccagacaac agcgatgtcg
     8161 acaaactctt tatccaactg gttcagactt acaatcagct tttcgaagag aacccgatca
     8221 acgcatccgg agttgacgcc aaagcaatcc tgagcgctag gctgtccaaa tcccggcggc
     8281 tcgaaaacct catcgcacag ctccctgggg agaagaagaa cggcctgttt ggtaatctta
     8341 tcgccctgtc actcgggctg acccccaact ttaaatctaa cttcgacctg gccgaagatg
     8401 ccaagcttca actgagcaaa gacacctacg atgatgatct cgacaatctg ctggcccaga
     8461 tcggcgacca gtacgcagac ctttttttgg cggcaaagaa cctgtcagac gccattctgc
     8521 tgagtgatat tctgcgagtg aacacggaga tcaccaaagc tccgctgagc gctagtatga
     8581 tcaagcgcta tgatgagcac caccaagact tgactttgct gaaggccctt gtcagacagc
     8641 aactgcctga gaagtacaag gaaattttct tcgatcagtc taaaaatggc tacgccggat
     8701 acattgacgg cggagcaagc caggaggaat tttacaaatt tattaagccc atcttggaaa
     8761 aaatggacgg caccgaggag ctgctggtaa agcttaacag agaagatctg ttgcgcaaac
     8821 agcgcacttt cgacaatgga agcatccccc accagattca cctgggcgaa ctgcacgcta
     8881 tcctcaggcg gcaagaggat ttctacccct ttttgaaaga taacagggaa aagattgaga
     8941 aaatcctcac atttcggata ccctactatg taggccccct cgcccgggga aattccagat
     9001 tcgcgtggat gactcgcaaa tcagaagaga ccatcactcc ctggaacttc gaggaagtcg
     9061 tggataaggg ggcctctgcc cagtccttca tcgaaaggat gactaacttt gataaaaatc
     9121 tgcctaacga aaaggtgctt cctaaacact ctctgctgta cgagtacttc acagtttata
     9181 acgagctcac caaggtcaaa tacgtcacag aagggatgag aaagccagca ttcctgtctg
     9241 gagagcagaa gaaagctatc gtggacctcc tcttcaagac gaaccggaaa gttaccgtga
     9301 aacagctcaa agaagactat ttcaaaaaga ttgaatgttt cgactctgtt gaaatcagcg
     9361 gagtggagga tcgcttcaac gcatccctgg gaacgtatca cgatctcctg aaaatcatta
     9421 aagacaagga cttcctggac aatgaggaga acgaggacat tcttgaggac attgtcctca
     9481 cccttacgtt gtttgaagat agggagatga ttgaagaacg cttgaaaact tacgctcatc
     9541 tcttcgacga caaagtcatg aaacagctca agaggcgccg atatacagga tgggggcggc
     9601 tgtcaagaaa actgatcaat gggatccgag acaagcagag tggaaagaca atcctggatt
     9661 ttcttaagtc cgatggattt gccaaccgga acttcatgca gttgatccat gatgactctc
     9721 tcacctttaa ggaggacatc cagaaagcac aagtttctgg ccagggggac agtcttcacg
     9781 agcacatcgc taatcttgca ggtagcccag ctatcaaaaa gggaatactg cagaccgtta
     9841 aggtcgtgga tgaactcgtc aaagtaatgg gaaggcataa gcccgagaat atcgttatcg
     9901 agatggcccg agagaaccaa actacccaga agggacagaa gaacagtagg gaaaggatga
     9961 agaggattga agagggtata aaagaactgg ggtcccaaat ccttaaggaa cacccagttg
    10021 aaaacaccca gcttcagaat gagaagctct acctgtacta cctgcagaac ggcagggaca
    10081 tgtacgtgga tcaggaactg gacatcaatc ggctctccga ctacgacgtg gatgccatcg
    10141 tgccccagtc ttttctcaaa gatgattcta ttgataataa agtgttgaca agatccgata
    10201 aaaatagagg gaagagtgat aacgtcccct cagaagaagt tgtcaagaaa atgaaaaatt
    10261 attggcggca gctgctgaac gccaaactga tcacacaacg gaagttcgat aatctgacta
    10321 aggctgaacg aggtggcctg tctgagttgg ataaagccgg cttcatcaaa aggcagcttg
    10381 ttgagacacg ccagatcacc aagcacgtgg cccaaattct cgattcacgc atgaacacca
    10441 agtacgatga aaatgacaaa ctgattcgag aggtgaaagt tattactctg aagtctaagc
    10501 tggtctcaga tttcagaaag gactttcagt tttataaggt gagagagatc aacaattacc
    10561 accatgcgca tgatgcctac ctgaatgcag tggtaggcac tgcacttatc aaaaaatatc
    10621 ccaagcttga atctgaattt gtttacggag actataaagt gtacgatgtt aggaaaatga
    10681 tcgcaaagtc tgagcaggaa ataggcaagg ccaccgctaa gtacttcttt tacagcaata
    10741 ttatgaattt tttcaagacc gagattacac tggccaatgg agagattcgg aagcgaccac
    10801 ttatcgaaac aaacggagaa acaggagaaa tcgtgtggga caagggtagg gatttcgcga
    10861 cagtccggaa ggtcctgtcc atgccgcagg tgaacatcgt taaaaagacc gaagtacaga
    10921 ccggaggctt ctccaaggaa agtatcctcc cgaaaaggaa cagcgacaag ctgatcgcac
    10981 gcaaaaaaga ttgggacccc aagaaatacg gcggattcga ttctcctaca gtcgcttaca
    11041 gtgtactggt tgtggccaaa gtggagaaag ggaagtctaa aaaactcaaa agcgtcaagg
    11101 aactgctggg catcacaatc atggagcgat caagcttcga aaaaaacccc atcgactttc
    11161 tcgaggcgaa aggatataaa gaggtcaaaa aagacctcat cattaagctt cccaagtact
    11221 ctctctttga gcttgaaaac ggccggaaac gaatgctcgc tagtgcgggc gagctgcaga
    11281 aaggtaacga gctggcactg ccctctaaat acgttaattt cttgtatctg gccagccact
    11341 atgaaaagct caaagggtct cccgaagata atgagcagaa gcagctgttc gtggaacaac
    11401 acaaacacta ccttgatgag atcatcgagc aaataagcga attctccaaa agagtgatcc
    11461 tcgccgacgc taacctcgat aaggtgcttt ctgcttacaa taagcacagg gataagccca
    11521 tcagggagca ggcagaaaac attatccact tgtttactct gaccaacttg ggcgcgcctg
    11581 cagccttcaa gtacttcgac accaccatag acagaaagcg gtacacctct acaaaggagg
    11641 tcctggacgc cacactgatt catcagtcaa ttacggggct ctatgaaaca agaatcgacc
    11701 tctctcagct cggtggagac agcagggctg accccaagaa gaagaggaag gtggctagcc
    11761 gcgccgacgc gctggacgat ttcgatctcg acatgctggg ttctgatgcc ctcgatgact
    11821 ttgacctgga tatgttggga agcgacgcat tggatgactt tgatctggac atgctcggct
    11881 ccgatgctct ggacgatttc gatctcgata tgttaatcgc tagcgagggc agaggaagtc
    11941 ttctaacatg cggtgacgtg gaggagaatc ccggccctgg taccatgacc gagtacaagc
    12001 ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg
    12061 cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg
    12121 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg
    12181 tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg
    12241 cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc
    12301 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg
    12361 ccaccgtcgg cgtgtcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc
    12421 ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc
    12481 gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg
    12541 aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgaccagca cactggcggc
    12601 cgttactagc ttctgcagca cgaccggttg ataatagata acttcgtata gcatacatta
    12661 tacgaagtta tgaattcgat atcaagctta tcgataatca acctctggat tacaaaattt
    12721 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
    12781 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
    12841 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
    12901 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
    12961 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
    13021 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
    13081 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc
    13141 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
    13201 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
    13261 tctccctttg ggccgcctcc ccgcatcgat accgtcgacc tcgggtacct ttaagaccaa
    13321 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
    13381 ggctaattca ctcccaacga agacaagata tccttgatct gtggatctac cacacacaag
    13441 gctacttccc tgattggcag aactacacac cagggccagg gatcagatat ccactgacct
    13501 ttggatggtg ctacaagcta gtaccagttg agcaagagaa ggtagaagaa gccaatgaag
    13561 gagagaacac ccgcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag
    13621 aagtattaga gtggaggttt gacagccgcc tagcatttca tcacatggcc cgagagctgc
    13681 atccggactg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
    13741 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
    13801 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
    13861 ggaaaatctc tagcagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt
    13921 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
    13981 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
    14041 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
    14101 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc
    14161 tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
    14221 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
    14281 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
    14341 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
    14401 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
    14461 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
    14521 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
    14581 atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa
    14641 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
    14701 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
    14761 attagtcagc aacc
//
