LOCUS       sequence-502730-       10174 bp    DNA     circular SYN 11-MAR-2026
DEFINITION  Insect Expression of DmGIGYF-WFFtoAAA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10174)
  AUTHORS   Ruscica V, Bawankar P, Peter D, Helms S, Igreja C, Izaurralde E
  TITLE     Direct role for the Drosophila GIGYF protein in 4EHP-mediated mRNA 
            repression.
  JOURNAL   Nucleic Acids Res. 2019 Jul 26;47(13):7035-7048. doi: 
            10.1093/nar/gkz429.
  PUBMED    31114929
REFERENCE   2  (bases 1 to 10174)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 11, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2019-07-26- 26"; volume: "47"; issue: 
            "13"; pages: "7035-7048"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmGIGYF-WFFtoAAA_AH
FEATURES             Location/Qualifiers
     source          1..10174
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2565
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2585..2650
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2675..2701
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             7480..7521
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             7531..7548
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7571..7588)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7621..7755
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7645..7664)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7699..7718
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(8356..8944)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8436..8455)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9115..9906,9907..9975))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9738..9757
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9976..10080)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10148..10166
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaagaat taattctata ttctaaaaac acaaatgata cttctaaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat aatgaatatg tacacattcg
      241 agaaagaagc gatcaaagaa gcgtcttcgg gcggagtagg agaatgcgga ggagaaggag
      301 aacgagctga tctagtatct ctccacaatc caatgccaac tgaccaactg gccatattcg
      361 gagcaatttg aagccaattt ccatcgcctg gcgatcgctc cattcttggc tatatgtttt
      421 tcaccgttac ccggggccat tttcaaagac tcgtcggcaa gataagattg tgtcactcgc
      481 tgtctctctt catttgtcga agaatgctga ggaatttcgc gatgacgtcg gcgagtattt
      541 tgaagaatga gaataatttg tatttatacg aaaatcagtt agtggaattt tctacaaaaa
      601 catgttatct atagataatt ttgttgcaaa atatgttgac tatgacaaag attgtatgta
      661 tataccttta atgtattctc attttcttat gtatttataa tggcaatgat gatactgatg
      721 atattttaag atgatgccag accaaaaggc ttgaatttct gcgtcttttg ccgaacgcag
      781 tgcatgtgca attgttgttt tttggaatat tcaattttcg gactgtccgc tttgatttca
      841 gtttcttggc ttattcaaaa agcaaagtaa agccaaaaaa gcgagatggc aataccaaat
      901 gcggcaaaac ggtagtggaa ggaaaggggt gcggggcagc ggaaggaagg gtggggcggg
      961 gcgtggcggg gtctgtggct gggcgcgacg tcaccgacgt tggagccact cctttgacca
     1021 tgtgtgcgtg tgtgtattat tcgtgtctcg ccactcgccg gttgtttttt tctttttatg
     1081 ctgcgctctc tctagcgcca tctcgcttac gcatgctcaa cgcaccgcat gttgccgttt
     1141 ccttttatgc gtcattttgg ctcgaaatag gcaattattt aaacaaagat tagtcaacga
     1201 aaacgctaaa ataaataagt ctacaatatg gttacttatt gccatgtgtg tgcagccaac
     1261 gatagcaaca aaagcaacaa cacaggtggc tttccctctt tcactttttg tttgcaagcc
     1321 gcgtgcgagc aagacggcac gaccggcaaa cgcaattacg ctgacaaaga gcagacgaag
     1381 ttttggcgaa aaacatcaag gcgcctgata cgaatgcatt tgcaataaca attgcgatat
     1441 ttaatattgt ttatgaagct gtttgacttc aaaacacaca aaaaaaaaaa taaaacaaat
     1501 tatttgaaag agaattagga atcggacgct tatcgttagg gtaacaacaa gaaatgctta
     1561 ctgagtcaca gcctctggaa aactgccgca agccagagag agagagaaaa agagggagag
     1621 cagcttagac cgcatgtgct tgtgtgtgag gcgtctctct cttcgtctct gttgcgcaaa
     1681 cgcatagact gcactgaaaa aatcgattac ctatttttta tgaatgaata tttgcactat
     1741 tactattcaa aactattaag atagcaatca cattcaatag ccaaatacta taccacctga
     1801 gcgatgcaac gaaatgatca atttgagcaa aaatgctgca tatttaggac ggcatcatta
     1861 tagaaatgct tcttgctgtg tacttttctc tcgtctggca gctgtttcgc cgttattgtt
     1921 aaaaccggct taagttaggt gtgttttcta cgactagtga atgccctact agaagatgtg
     1981 tgttgcacaa aatgtccctg gaataaccaa tttgaagtgc agatagcagt aaacgtaagc
     2041 taatatgaat attatttaac tgtaatgttt taatatcgct ggacattact aataaaccca
     2101 ctataaacac atgtacatat gtatgttttg gcatacaatg agtagttggg gaaaaaatgt
     2161 gtaaaagcac cgtgaccatc acagcataaa gataaccagc tgaagtatcg aatatgagta
     2221 acccccaaat tgaatcacat gccgcaactg ataggaccca tggaagtaca ctcttcatgg
     2281 cgatatacaa gacacacaca agcacgaaca cccagttgcg gaggaaattc tccgtaaatg
     2341 aaaacccaat cggcgaacaa ttcataccca tatatggtaa aagttttgaa cgcgacttga
     2401 gagcggagag cattgcggct gataaggttt tagcgctaag cgggctttat aaaacgggct
     2461 gcgggaccag ttttcatatc actaccgttt gagttcttgt gctgtgtgga tactcctccc
     2521 gacacaaagc cgctccatca gccagcagtc gtctaatcca gagaccccgg atcggggtac
     2581 caacatggac gcacaaacac gacgacgtga gcgtcgcgct gagaaacaag ctcaatggaa
     2641 agctgcaaac ggtggatctc ctccacatat ggcttaccca tacgatgttc cagattacgc
     2701 tcctccaagc ttgaattcta tgacagattc aatgaaattt ggcccggaat ggttgcgcaa
     2761 tatgtcagcc gagccttcgg gctctcccag tacctacaac gttggtaatg cagctcaaaa
     2821 caattcgatt ggagggcaca acctgggaaa caacacggcg gcatccgctt cccgtaatct
     2881 atttccagaa taccggtacg gacgcgagga aatgctgtcc ttgttcgatc ggaactgcct
     2941 actgccgcag atcctgccat cgttcaaaaa gctcttcgtg gaaaaggttc agtgccctct
     3001 tgcactgaca ccgagctccg aggaggacat caaccaaaac tcacttggca ataactctcg
     3061 tcctgcctgg ttgcagcgct cgcccagtgg atttggtagt gcctcccgag gctctggacg
     3121 tggtggaacc gttgaccggg gacggatgcg cggaaaatcg gcctatcatc cgatatacca
     3181 acgcccaagc ggtctatatg atgaaagctt atcggttata tcaaaggccg aacgcacatg
     3241 gagcgaccgc aacggaactg gagattctgc ggcaaccacc acctccacta gtggctctgg
     3301 tgctctagat tggaatggaa ctccaagctc aagtcctcga aaagattatt ctaataatca
     3361 tcgcaacttg gaaaactggc gacgaacacg taacgaagat ggatccggtg atggtccatc
     3421 ctccagcggt tccatgagcg gacccgatat tgctggttgg cggagcggtg tcgtcggtgg
     3481 aagtacaaac gcaggttttg gtaccaatag ccatcggtgg ggaagatcta caagctggcg
     3541 tgatgaagat cctagcgtcg acaatgcagc tagtttacaa cgctcaattt caacagttgc
     3601 gactttaagt acagatcgga ctggaaacaa taaaggatct ggaatcggag ctgcagaggg
     3661 agtagggagc atatcgcacc cacggttgtc gagttctaaa atatctcaat tatggactgt
     3721 taataatgca gccggtgttg atgccgacga aaatctaccc gaagcggcaa ttgaaaatcc
     3781 atcgaaattg ggtggcagtg ctgatgctag tggagccgct catggagata ctgatctaaa
     3841 acctatcaaa agttcgcata atactttaaa aaccaaaagc ttagactcct atgacgatgt
     3901 aaaaagaccg aaaagtaaag acctgtctga tccagatagt ggaaacgata taactccaga
     3961 aacctcatta acgaaagata ccaatacaac agctgtgcaa gaagaagttg aaagcagttt
     4021 atctccaata agctctacaa cgacaaagga agtgatccat ggagatattt cagatcggat
     4081 taaagaggtc gccgatgaag tagaaaaact tataatggat gatgatcata aaatctcttc
     4141 gaatcaaagc caacatcaaa atcatgacgg gtttacagct gcacttccaa gaatggcaga
     4201 cattgagata aacgtaaaac cgagtgttac agcagtgcat cggcaagcac cttccaccat
     4261 gcctatacaa attaccccta caattacaga tgtcgctcca ccatcccatg cagttgtgtc
     4321 gttttcggat catgagacaa tgcaacacca taacatgcac cacctgccac aatttccaat
     4381 gattcctaca ccgcatatca tcactccaaa tctaaacgaa ttgtggtttt accgggatcc
     4441 gcaggcaaat gttcaggggc catttagtgc cgttgagatg acggaatggt atcgcgctgg
     4501 ctactttaat gagaacctgt ttgtacgccg gtactctgat aataggttta gaccgctggg
     4561 agagcttata aaattttgtc atggtaacat gccatttacg cacagtcacc tacttccatc
     4621 tccgatagaa ctagagaaca ttcctgttgg tcaaatacca gcccctctaa cggcgtcact
     4681 ttcaattaca ccccataagc catcaccgat tcctatcgca ctttctgttg ttgaacagca
     4741 gttgcagcaa caaagagatg agcaactaaa ggcaaatgtt acagcaacag ctgaagctct
     4801 acgtgctgca ataaaaggaa gttttggcgg aaatagcatt ggtaatacgt ctcatttgct
     4861 tacaatgcgg tttcaaatgc tacaggatca gtacatacag caccaggaat accaaatact
     4921 agctgagcta tccaaaaatg aatgctttca acgtctttcg gctgtcgagc aagaaacagt
     4981 tgttcgtcgg aaagttcaat tgctgggtct tcctgagtat ttgatcagct taaacggatt
     5041 aagcaattct ttgtccgtac tgaaccccgt tgccggtaga cagttgtaca gggcagtggt
     5101 tgagcacgcc aagaaggatc aacaacatat ttttgcaaac accgagcaac aacgttcagt
     5161 gggaaattta ctggatgcta ataatttcat tctaaatgcc caaataatga ttcagcaatc
     5221 gcagcaagag gtaggtccct tggtatcatc cgtggattgt attatgcaag gtggaactgc
     5281 agctgaccta aataagccga atgaaatacc gaggaatgag ttagacttaa ttaatgaata
     5341 taacttacgg atgctgttgc gtggccaacc aacaagtact caacagcaac cacctgcgct
     5401 gacgaactca gctaccgaaa atctccctgg agttgatttt ttaactgaaa cacaattgtt
     5461 agagcggcaa aacttgatga ttccaatctg gttaccccct aataataata agcaacaaga
     5521 aaccgaccaa cagtgggctg aaatgtctaa tgcggatgca tcattatggg aaacggccaa
     5581 cttaaatgaa gagcgaaatg aagatcagca actattaata caaaagtctt ctgaagcgtg
     5641 cttcgcagat acagaaaaag atgtcaaaat tgcccaatta tttcaagttc aatcaggaaa
     5701 tgttgttaac cacaccgcat tagaggaatt agatcagtcc cctcaaaatt taaaaggttc
     5761 acataatcaa aaaatagtca aatcccttgt ttccgatatt caacagaatc ataatgagga
     5821 actaaattca catcaacacc aggtaaagca ggctaacaag cagaatctga atacaaaaca
     5881 gaatgcggca cagtcagccc taaagccaat taataacgaa aatgatcgca aacgagaaca
     5941 gacagaagaa aaaaaaaggc aaagagagga acgcaagcgc cagcaattgg aagatgataa
     6001 acgtcgggcg ttgaatgaat ctgaagaaca gacccgccaa attcaagagg aaaaggaaag
     6061 gcaacagcaa atacaagccc aacgtcgaaa ggcattgttg ggcaatgtcc attctctatc
     6121 agttcaaaat gggatgtcag gaacattagc gtctgcacaa agcaagaaga acgacgatgc
     6181 caaaacagca gaaccccaag tatcctcgcg cttgccgtct acatccgtag cgccttggtc
     6241 ttttcagttg caaaattcta tgagatcggc gcctggtctg gcagaaatac aaaaggcaga
     6301 acgtcgagag cgtcgcgcag accaacagcg acatcaagag ctattagaca agcaattgcg
     6361 tgccaatgcc gcagctgcgg ctgaagccaa cgatgctttg cttaaatggc agtcaacacc
     6421 agcgtcggcg cccgtaatga gtctcgccga aattcaagca gaagaggcaa ggcggttggc
     6481 caatgacctt gtggatcggc agcgtcgacg agaactggaa catcaccaac aagctccttt
     6541 gtcatcagcg gttttggtta cgagtgcaac ttccaacatc tggggtaacg ccaataaagc
     6601 atggagttcg tccgctgctc aatcactttc gttaaaaaca agttctggaa ctggtctgtg
     6661 ggacgaaccg aatccattag gttctaatgg atctggaaca agcggtacct catccgtaac
     6721 tgcggcggca gtcttggcag gaggattaaa ctcagctaat aaatccactc tgcaagctca
     6781 aaataagtcg tcggctttat ttgcatcgcc tcgaaatttg cgcaagagtc aaacattgcc
     6841 agcccttagt aacccagaaa agtcaaataa aaatggacca ggtcaacggc cagaaaaaca
     6901 aaagttggct caaactcgtt caaagggtgc cgctgtctca attgaagaga aggatagaga
     6961 aagaaaattg aatgcaaaaa gccagcaaag cagcaccgac caagccatta gcaaggttaa
     7021 tgagtatgaa aacgagttca ctagctggtg cataaagagc ctagataata tgtccgccaa
     7081 agttgatgta cccacgttcg ttgcattctt gcaggacttg gaagcgccat atgaggtaaa
     7141 ggactatgtc cgaatatacc ttggcgatgg aaaagattct ttggatttcg caaagcagtt
     7201 tttggagcga cgtagcaaat acaaaagctt gcaacgtgcc caaaaggcac acaatgacga
     7261 tatgtgcaaa ccagctcctg ccattacgcc atctgcgaat gactatgccg acagcaagaa
     7321 caaacaaaaa aagattaaaa agaataagat gactaagatg gacgcccgca ttcttgggtt
     7381 ttcagtaact gctgccgagg gtcgcataaa tgttggcatt cgagactacg tcgaaggacc
     7441 ataagcggcc gctcgagtct agagggcccg cggttcgaag gtaagcctat ccctaaccct
     7501 ctcctcggtc tcgattctac gcgtaccggt catcatcacc atcaccattg agtttaaacc
     7561 cgctgatcag cctcgactgt gccttctaag gcctgagctc gctgatcagc ctcgatcgag
     7621 gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga
     7681 aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc
     7741 tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag
     7801 gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtatggc tgattatgat
     7861 cagtcgaccg atgcccttga gagccttcaa cccagtcagc tccttccggt gggcgcgggg
     7921 catgactatc gtcgccgcac ttatgactgt cttctttatc atgcaactcg taggacaggt
     7981 gccggcagcg ctctgggtca ttttcggcga ggaccgcttt cgctggagcg cgacgatgat
     8041 cggcctgtcg cttgcggtat tcggaatctt gcacgccctc gctcaagcct tcgtcactgg
     8101 tcccgccacc aaacgtttcg gcgagaagca ggccattatc gccggcatgg cggccgacgc
     8161 gctgggctac gtcttgctgg cgttcgcgac gcgaggctgg atggccttcc ccattatgat
     8221 tcttctcgct tccggcggca tcgggatgcc cgcgttgcag gccatgctgt ccaggcaggt
     8281 agatgacgac catcagggac agcttcaagg ccagcaaaag gccaggaacc gtaaaaaggc
     8341 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     8401 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     8461 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     8521 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     8581 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     8641 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     8701 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     8761 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     8821 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     8881 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     8941 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     9001 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     9061 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     9121 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     9181 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     9241 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     9301 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     9361 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     9421 tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     9481 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     9541 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     9601 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     9661 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     9721 cccggcgtca acacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     9781 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     9841 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     9901 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     9961 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
    10021 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
    10081 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
    10141 ctataaaaat aggcgtatca cgaggccctt tcgt
//
