LOCUS       sequence-502903-        5044 bp    DNA     circular SYN 26-MAR-2026
DEFINITION  5' entry vector containing the zebrafish krt5 promoter for 
            epidermis-specific expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5044)
  AUTHORS   Akerberg AA, Stewart S, Stankunas K
  TITLE     Spatial and temporal control of transgene expression in zebrafish.
  JOURNAL   PLoS One. 2014 Mar 18;9(3):e92217. doi: 
            10.1371/journal.pone.0092217. eCollection 2014.
  PUBMED    24643048
REFERENCE   2  (bases 1 to 5044)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 26, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-03-18"; volume: "9(3):e92217. doi"; pages: " 
            10.1371/journal.pone.0092217. eCollection 2014"
COMMENT     Sequence Label: p5E-krt5
FEATURES             Location/Qualifiers
     source          1..5044
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    593..688
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        690..708
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3018..3034)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3018..3034)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(3071..3089)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    3090..3213
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(3286..3304)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3309..3325)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3309..3325)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3427..3446)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3438..4247
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3505..3524)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4394..4982
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4883..4902
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccga gttaacgcta ccatggagct ccaaataatg
      601 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgataagca atgctttttt
      661 ataatgccaa ctttgtatag aaaagttggt aatacgactc actatagggc gaattgggta
      721 ccgggccccc cctcgaggtt cgaacagtgt attgtagttc ctcaaaatag caacgcgcca
      781 gcaatgcgcc tcaacacgcc tcctttttta gaccaaaacg tctattggca cacatactgt
      841 atgagcgcta atgcatttgc tatttaaaca gcgtggtgca aaacgtcaaa atgactgttg
      901 cactgagcta aaactacaat tgcgtcgcgc cttgcgccgg gtgtatgata gggccccaag
      961 agtttattta cttatttaag tgccttggtc cccaatactg aatttactag atcaaaatta
     1021 caatacaacc tttatacttc caaaaacatt atatgtaaat atagttcaaa atataattta
     1081 ttcttatgat ggcaacgcta aatttttagc attgtagttt taaatgtcat gatactgtaa
     1141 aagtcattta aatatggtgg tctgatgctc aaaaatccct ttttattgta tgagttgtgc
     1201 tacttcaaac tgtatttttt atgtaaacgc tcactaaaaa tgtttgactg tcaggaacat
     1261 taaaaatcga cagtaaaagt cattaaaaat gttattaaac atttcagttc taataactac
     1321 tgtttgttaa caatctattc atagatattt taactgtgca gcaaatcaac agtgcctatt
     1381 tttattttat atttgatata ataatagcct aataacagat aataaaacct ggatgaacag
     1441 aagagacaaa atgtcctaat gtctccaaaa ctttaacaga tggttctgca agggaaatgt
     1501 ttacaaccat gaaagcattt caaatattta atgagtttga tcattagcaa agcaaacacg
     1561 caaggtaaat ttcaaaccag tttcacgtga tcaacctgca catgttacac ttacctgttt
     1621 ttttaaccac atatatggtt tctgggtctt caagacctct tttatctcac aacctaatct
     1681 acattttgta acttgtacta ctcgattggt tgcattgcat gaattaaggg gaaatacttg
     1741 ggtggaactc atcaacccca acatctgata gtgtcactgt tttgaccaac ggattaacaa
     1801 tgggctaaac ccgtttaaga aaaaaaaaac tgcacagatg gccaaaatac cacataagca
     1861 atgtttgtta aaagtgcgac ccctcctaaa tttttacaga gaaatatgcc ttattttcct
     1921 tttgcttccc tgctaatatg ctaatgctct aaagtaaaca aaccagacag aatctcttcc
     1981 tgctgtactt ggtgtgatga acatttacaa ggctgaaagc agactcagat ctgtccactt
     2041 tagaagcaag actccagttc gaaatcattt aagcatgata cgggtgatag tgacatgcta
     2101 acaaaagcag cacgcgttgc taaataatgc cacctaagga caggtacaaa aataaccaca
     2161 ctgctcttgc agccattgca cactgctgtg agctctgaca ctcacctcat aaaattctaa
     2221 tctcacacga taatgaaaac tatgctgtac acattcacgt ttgcgtaaga taataaaaat
     2281 ctactttgca tgcaactgcg tcagccctct gggaaccaca gaacaaggtg gtgaagcagt
     2341 gtccgtgaca gtgccattga agtgagagaa cagaagcttg attgtttgac tggaaagaga
     2401 agcctgtggg tcactatggc ttccaggatg agcgccaagt ctcaacattc aagcccttca
     2461 tcaaatattc ccctatttaa tactcctgca ttgcacttaa agcaaactga gggccccata
     2521 cctgcatgaa tcataaaata ttcatggagt tgtatggttt gcatttggag aatgccatct
     2581 ggttatgtaa ataagagggg ctttaaatta cagagaactt gtatattctg agtcgtagta
     2641 catacaaggg tgtgtggagt ttaaccttgt ttttctactc tgtttctctc ttaatagctc
     2701 tgttttatta cgtgagtatg catgtgcatc taaggcaggg tgtggcctgg cctccaatgc
     2761 tgaacatact cccataccag cccagcaaga tgggcgtgtg tatcacttgg acagggaaaa
     2821 aggcaggaca gaagcctgag gcagaaaggg gattggccag gtgagcccct cccatgaggt
     2881 aagaggtata aaagccactg cactgcttga agagggcact cagagggatt ttactttcct
     2941 cttagtgagc tctcctgctc cagatctctg tctcatcctg ctctttttct gatcctcacc
     3001 ccctcgtaga cgacagggat ccactagttc tagagcggcc gccaccgcgg tggagctcca
     3061 gcttttgttc cctttagtga gggttaattc aagtttgtac aaaaaagttg aacgagaaac
     3121 gtaaaatgat ataaatatca atatattaaa ttagattttg cataaaaaac agactacata
     3181 atactgtaaa acacaacata tgcagtcact atgaaccaac tacttagatg gtattagtga
     3241 cctgtagaat tcgagctcta gagctgcagg gcggccgcga tatcccctat agtgagtcgt
     3301 attacatggt catagctgtt tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt
     3361 acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca
     3421 gtaatacaag gggtgttatg agccatattc aacgggaaac gtcgaggccg cgattaaatt
     3481 ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc gggcaatcag
     3541 gtgcgacaat ctatcgcttg tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg
     3601 gcaaaggtag cgttgccaat gatgttacag atgagatggt cagactaaac tggctgacgg
     3661 aatttatgcc tcttccgacc atcaagcatt ttatccgtac tcctgatgat gcatggttac
     3721 tcaccactgc gatccccgga aaaacagcat tccaggtatt agaagaatat cctgattcag
     3781 gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg attcctgttt
     3841 gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga
     3901 ataacggttt ggttgatgcg agtgattttg atgacgagcg taatggctgg cctgttgaac
     3961 aagtctggaa agaaatgcat aaacttttgc cattctcacc ggattcagtc gtcactcatg
     4021 gtgatttctc acttgataac cttatttttg acgaggggaa attaataggt tgtattgatg
     4081 ttggacgagt cggaatcgca gaccgatacc aggatcttgc catcctatgg aactgcctcg
     4141 gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt gataatcctg
     4201 atatgaataa attgcagttt catttgatgc tcgatgagtt tttctaatca gaattggtta
     4261 attggttgta acactggcag agcattacgc tgacttgacg ggacggcgca agctcatgac
     4321 caaaatccct taacgtgagt tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga
     4381 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     4441 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     4501 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     4561 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     4621 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     4681 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     4741 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     4801 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     4861 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     4921 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     4981 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     5041 tgtt
//
