LOCUS       sequence-502937-       12136 bp    DNA     circular SYN 13-MAR-2026
DEFINITION  TRE-dCas9-KRAB knock-in using TALEN at CLYBL locus (derived from 
            Plasmid #220439).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12136)
  TITLE     Additional TF Perturb Seq benchmarking Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12136)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 13, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: CYLBL_Neo_TREdCas9-KRAB
FEATURES             Location/Qualifiers
     source          1..12136
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    33..51
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    69..87
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    105..123
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    141..159
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    177..195
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    213..231
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    249..267
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     312..336
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             439..486
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             join(487..510,511..513,514..3069,3070..3072,3073..4587)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the N863A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKARGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4642..4662
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4705..4899
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQILYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEPWLV"
     CDS             4909..4929
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(5022..5041)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5087..5142
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5141..5160)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     protein_bind    complement(7050..7083)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    7084..7109
                     /label=SA
                     /note="splice acceptor site"
     CDS             7133..7186
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             7190..7783
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     7683..7703
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             7790..8785
                     /codon_start=1
                     /product="truncated herpes simplex virus thymidine kinase 
                     (Salomon et al., 1995)"
                     /label=Delta-TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWQV
                     LGASETIANIYTTQHRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGS
                     SHAPPPALTLIFDRHPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGAL
                     PEDRHIDRLAKRQRPGERLDLAMLAAIRRVYGLLANTVRYLQGGGSWREDWGQLSGTAV
                     PPQGAEPQSNAGPRPHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRPMHVFI
                     LDYDQSPAGCRDALLQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     polyA_signal    8813..9037
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        9073..9284
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             9297..9565
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      9603..9612
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(9754..9772)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     10022..10041
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    10370..10594
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        10619..10682
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10683..10751,10752..11543)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10901..10920)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(11566..12136,1..18)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12055..12074
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gggcggagcc tatggaaaaa catgcatttt actccctatc agtgatagag aacgtatgaa
       61 gagtttactc cctatcagtg atagagaacg tatgcagact ttactcccta tcagtgatag
      121 agaacgtata aggagtttac tccctatcag tgatagagaa cgtatgacca gtttactccc
      181 tatcagtgat agagaacgta tctacagttt actccctatc agtgatagag aacgtatatc
      241 cagtttactc cctatcagtg atagagaacg tataagcttt aggcgtgtac ggtgggcgcc
      301 tataaaagca gagctcgttt agtgaaccgt cagatcgcct ggagcaattc cacaacactt
      361 ttgtcttata ccaactttcc gtaccacttc ctaccctcgt aaagctagcg cccgggcgtc
      421 gaccgtacgg ccaccatgaa aaggccggcg gccacgaaaa aggccggcca ggcaaaaaag
      481 aaaaaggaca agaagtacag catcggcctg gccatcggca ccaactctgt gggctgggcc
      541 gtgatcaccg acgagtacaa ggtgcccagc aagaaattca aggtgctggg caacaccgac
      601 cggcacagca tcaagaagaa cctgatcgga gccctgctgt tcgacagcgg cgaaacagcc
      661 gaggccaccc ggctgaagag aaccgccaga agaagataca ccagacggaa gaaccggatc
      721 tgctatctgc aagagatctt cagcaacgag atggccaagg tggacgacag cttcttccac
      781 agactggaag agtccttcct ggtggaagag gataagaagc acgagcggca ccccatcttc
      841 ggcaacatcg tggacgaggt ggcctaccac gagaagtacc ccaccatcta ccacctgaga
      901 aagaaactgg tggacagcac cgacaaggcc gacctgcggc tgatctatct ggccctggcc
      961 cacatgatca agttccgggg ccacttcctg atcgagggcg acctgaaccc cgacaacagc
     1021 gacgtggaca agctgttcat ccagctggtg cagacctaca accagctgtt cgaggaaaac
     1081 cccatcaacg ccagcggcgt ggacgccaag gccatcctgt ctgccagact gagcaagagc
     1141 agacggctgg aaaatctgat cgcccagctg cccggcgaga agaagaatgg cctgttcggc
     1201 aacctgattg ccctgagcct gggcctgacc cccaacttca agagcaactt cgacctggcc
     1261 gaggatgcca aactgcagct gagcaaggac acctacgacg acgacctgga caacctgctg
     1321 gcccagatcg gcgaccagta cgccgacctg tttctggccg ccaagaacct gtccgacgcc
     1381 atcctgctga gcgacatcct gagagtgaac accgagatca ccaaggcccc cctgagcgcc
     1441 tctatgatca agagatacga cgagcaccac caggacctga ccctgctgaa agctctcgtg
     1501 cggcagcagc tgcctgagaa gtacaaagag attttcttcg accagagcaa gaacggctac
     1561 gccggctaca ttgacggcgg agccagccag gaagagttct acaagttcat caagcccatc
     1621 ctggaaaaga tggacggcac cgaggaactg ctcgtgaagc tgaacagaga ggacctgctg
     1681 cggaagcagc ggaccttcga caacggcagc atcccccacc agatccacct gggagagctg
     1741 cacgccattc tgcggcggca ggaagatttt tacccattcc tgaaggacaa ccgggaaaag
     1801 atcgagaaga tcctgacctt ccgcatcccc tactacgtgg gccctctggc caggggaaac
     1861 agcagattcg cctggatgac cagaaagagc gaggaaacca tcaccccctg gaacttcgag
     1921 gaagtggtgg acaagggcgc ttccgcccag agcttcatcg agcggatgac caacttcgat
     1981 aagaacctgc ccaacgagaa ggtgctgccc aagcacagcc tgctgtacga gtacttcacc
     2041 gtgtataacg agctgaccaa agtgaaatac gtgaccgagg gaatgagaaa gcccgccttc
     2101 ctgagcggcg agcagaaaaa ggccatcgtg gacctgctgt tcaagaccaa ccggaaagtg
     2161 accgtgaagc agctgaaaga ggactacttc aagaaaatcg agtgcttcga ctccgtggaa
     2221 atctccggcg tggaagatcg gttcaacgcc tccctgggca cataccacga tctgctgaaa
     2281 attatcaagg acaaggactt cctggacaat gaggaaaacg aggacattct ggaagatatc
     2341 gtgctgaccc tgacactgtt tgaggacaga gagatgatcg aggaacggct gaaaacctat
     2401 gcccacctgt tcgacgacaa agtgatgaag cagctgaagc ggcggagata caccggctgg
     2461 ggcaggctga gccggaagct gatcaacggc atccgggaca agcagtccgg caagacaatc
     2521 ctggatttcc tgaagtccga cggcttcgcc aacagaaact tcatgcagct gatccacgac
     2581 gacagcctga cctttaaaga ggacatccag aaagcccagg tgtccggcca gggcgatagc
     2641 ctgcacgagc acattgccaa tctggccggc agccccgcca ttaagaaggg catcctgcag
     2701 acagtgaagg tggtggacga gctcgtgaaa gtgatgggcc ggcacaagcc cgagaacatc
     2761 gtgatcgaaa tggccagaga gaaccagacc acccagaagg gacagaagaa cagccgcgag
     2821 agaatgaagc ggatcgaaga gggcatcaaa gagctgggca gccagatcct gaaagaacac
     2881 cccgtggaaa acacccagct gcagaacgag aagctgtacc tgtactacct gcagaatggg
     2941 cgggatatgt acgtggacca ggaactggac atcaaccggc tgtccgacta cgatgtggac
     3001 cacatcgtgc ctcagagctt tctgaaggac gactccatcg acaacaaggt gctgaccaga
     3061 agcgacaagg cccggggcaa gagcgacaac gtgccctccg aagaggtcgt gaagaagatg
     3121 aagaactact ggcggcagct gctgaacgcc aagctgatta cccagagaaa gttcgacaat
     3181 ctgaccaagg ccgagagagg cggcctgagc gaactggata aggccggctt catcaagaga
     3241 cagctggtgg aaacccggca gatcacaaag cacgtggcac agatcctgga ctcccggatg
     3301 aacactaagt acgacgagaa tgacaagctg atccgggaag tgaaagtgat caccctgaag
     3361 tccaagctgg tgtccgattt ccggaaggat ttccagtttt acaaagtgcg cgagatcaac
     3421 aactaccacc acgcccacga cgcctacctg aacgccgtcg tgggaaccgc cctgatcaaa
     3481 aagtacccta agctggaaag cgagttcgtg tacggcgact acaaggtgta cgacgtgcgg
     3541 aagatgatcg ccaagagcga gcaggaaatc ggcaaggcta ccgccaagta cttcttctac
     3601 agcaacatca tgaacttttt caagaccgag attaccctgg ccaacggcga gatccggaag
     3661 cggcctctga tcgagacaaa cggcgaaacc ggggagatcg tgtgggataa gggccgggat
     3721 tttgccaccg tgcggaaagt gctgagcatg ccccaagtga atatcgtgaa aaagaccgag
     3781 gtgcagacag gcggcttcag caaagagtct atcctgccca agaggaacag cgataagctg
     3841 atcgccagaa agaaggactg ggaccctaag aagtacggcg gcttcgacag ccccaccgtg
     3901 gcctattctg tgctggtggt ggccaaagtg gaaaagggca agtccaagaa actgaagagt
     3961 gtgaaagagc tgctggggat caccatcatg gaaagaagca gcttcgagaa gaatcccatc
     4021 gactttctgg aagccaaggg ctacaaagaa gtgaaaaagg acctgatcat caagctgcct
     4081 aagtactccc tgttcgagct ggaaaacggc cggaagagaa tgctggcctc tgccggcgaa
     4141 ctgcagaagg gaaacgaact ggccctgccc tccaaatatg tgaacttcct gtacctggcc
     4201 agccactatg agaagctgaa gggctccccc gaggataatg agcagaaaca gctgtttgtg
     4261 gaacagcaca agcactacct ggacgagatc atcgagcaga tcagcgagtt ctccaagaga
     4321 gtgatcctgg ccgacgctaa tctggacaaa gtgctgtccg cctacaacaa gcaccgggat
     4381 aagcccatca gagagcaggc cgagaatatc atccacctgt ttaccctgac caatctggga
     4441 gcccctgccg ccttcaagta ctttgacacc accatcgacc ggaagaggta caccagcacc
     4501 aaagaggtgc tggacgccac cctgatccac cagagcatca ccggcctgta cgagacacgg
     4561 atcgacctgt ctcagctggg aggcgacagc gctggaggag gtggaagcgg aggaggagga
     4621 agcggaggag gaggtagcgg acctaagaaa aagaggaagg tggcggccgc tggatccgat
     4681 gctaagtcac tgactgcctg gtcccggaca ctggtgacct tcaaggatgt gtttgtggac
     4741 ttcaccaggg aggagtggaa gctgctggac actgctcagc agatcctgta cagaaatgtg
     4801 atgctggaga actataagaa cctggtttcc ttgggttatc agcttactaa gccagatgtg
     4861 atcctccggt tggagaaggg agaagagccc tggctggtgg agagagaacc gaaaaagaaa
     4921 cgcaaagttt gtacgcgtga attcactcct caggtgcagg ctgcctatca gaaggtggtg
     4981 actggtgtgg ccaatgccct ggctcacaaa taccactgag atctttttcc ctctgccaaa
     5041 aattatgggg acatcatgaa gccccttgag catctgactt ctggctaata aaggaaattt
     5101 attttcattg caatagtgtg ttggaatttt ttgtgtctct cactcggaag gacatatggg
     5161 agggcaaatc atttaaaaca tcagaatgag tatttggttt agagtttggc aacatatgcc
     5221 catatgctgg ctgccatgaa caaaggttgg ctataaagag gtcatcagta tatgaaacag
     5281 ccccctgctg tccattcctt attccataga aaagccttga cttgaggtta gatttttttt
     5341 atattttgtt ttgtgttatt tttttcttta acatccctaa aattttcctt acatgtttaa
     5401 ttaaatctcc tcatccttcc aacataaata tattttggga ttatatcaac attcaatgtt
     5461 acttaaagtg accttgtaaa tattttcaca actgagccat gtttgatttg tatacttatg
     5521 tttactttac tgtttttcct gaagttaata attgccttga atttatttat ttctttaaaa
     5581 atgtttcatt actcaggact gtagtttaca ttacgattct ttgtgttata cagttgatgg
     5641 gtttcttttc tttcttaatt tctttaaaaa atagagatgg ggtcttacta tattacccag
     5701 gctggtcttg aagtcctggg ctcaagtgat cttcctgtct cagcctacca agtagctgag
     5761 actataggtg caaaaaagcc actatacctg gctagtttac aggttttaac aaatgcatta
     5821 tgccacgtat ccattattac aggatcacac aagatatttt cattaccctg aaagcatccc
     5881 tgtgttccac caattcatcc tgcctccatg agccgctggc aaccactgat ctctatagtt
     5941 ttgccttttc taaaatgtca tataattgga atcatacagt ctgtagcatt ttcagactag
     6001 cttttaaaat ttggcaatat gcatttaagg ttcctcctta aatgtgaagg gcatagccaa
     6061 tgtggcttga tagctcattt ctttttattg gtgaatattt cattgtctgg atgttccaca
     6121 gtttgtttat ccattcacct attcaatttg ctttttttct gtgtatctat cactaattca
     6181 atactggact ctccaacaga gcccctagga gcaggagaat catgccaatg ggccaatata
     6241 cattctgacc cacagtttca taataaaata aaatggttgt ggttgtaagc cactatgttt
     6301 cagagtggtt tgttacacag caataaataa ctaatatagt aggcatacca tcaagtccaa
     6361 agtaggtaga gaagaatgta aatagcagag caaaacagca tgactggtgg ctgggaggct
     6421 taaaactggg acaggatcag agtcatgaaa gaagtcaaag aaatggttca gaagtaaggc
     6481 tgagactgac ttacaaaagc tgaaagtccc tttaagttgg tgtttggtgc attggcaggg
     6541 gcaggtatgg tgacttaaaa gagccatgct caacaagatc aagcacaaca caatcacggg
     6601 tcaccccagc agaccttagc gagtctagcc atttctttgg tggtggtcac agtcatgctt
     6661 cagcccagtt tccacttgga caaatggtac atattttcaa tgagatgaaa attaagatac
     6721 aatccatgtg ctcagagagt gatcacagct ctgactaaac actgtgcccc aaagtgttga
     6781 ggaattggga aaacctagct gagttagtgg tctcttttct gttacaataa agctcataat
     6841 gaaaattagc cttctttgtt cttccccaag tctttctttc tagacgaaac tactttcaac
     6901 tgttttaact tccttactgt taacttccat atttctagat agtatggtca gactgttatt
     6961 tcttgacttt taaatgtaag atattatcta ctgacttcct tctatgtaag atgaggattg
     7021 agctctctta cccttctccc agcggccgca taacttcgta tagcatacat tatacgaagt
     7081 tatctgacct cttctcttcc tcccacaggg cctcgagaga tctggcagcg gagagggcag
     7141 aggaagtctt ctaacatgcg gtgacgtgga ggagaatccc ggccctggga ccgagtacaa
     7201 gcccacggtg cgcctcgcca cccgcgacga cgtcccccgg gccgtacgca ccctcgccgc
     7261 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgac ccggaccgcc acatcgagcg
     7321 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg
     7381 ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg
     7441 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc
     7501 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct
     7561 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct
     7621 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc
     7681 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc
     7741 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gccggatcca tgcccacgct
     7801 actgcgggtt tatatagacg gtcctcacgg gatggggaaa accaccacca cgcaactgct
     7861 ggtggccctg ggttcgcgcg acgatatcgt ctacgtaccc gagccgatga cttactggca
     7921 ggtgctgggg gcttccgaga caatcgcgaa catctacacc acacaacacc gcctcgacca
     7981 gggtgagata tcggccgggg acgcggcggt ggtaatgaca agcgcccaga taacaatggg
     8041 catgccttat gccgtgaccg acgccgttct ggctcctcat atcggggggg aggctgggag
     8101 ctcacatgcc ccgcccccgg ccctcaccct catcttcgac cgccatccca tcgccgccct
     8161 cctgtgctac ccggccgcgc gataccttat gggcagcatg accccccagg ccgtgctggc
     8221 gttcgtggcc ctcatcccgc cgaccttgcc cggcacaaac atcgtgttgg gggcccttcc
     8281 ggaggacaga cacatcgacc gcctggccaa acgccagcgc cccggcgagc ggcttgacct
     8341 ggctatgctg gccgcgattc gccgcgttta cgggctgctt gccaatacgg tgcggtatct
     8401 gcagggcggc gggtcgtggc gggaggattg gggacagctt tcggggacgg ccgtgccgcc
     8461 ccagggtgcc gagccccaga gcaacgcggg cccacgaccc catatcgggg acacgttatt
     8521 taccctgttt cgggcccccg agttgctggc ccccaacggc gacctgtaca acgtgtttgc
     8581 ctgggccttg gacgtcttgg ccaaacgcct ccgtcccatg cacgtcttta tcctggatta
     8641 cgaccaatcg cccgccggct gccgggacgc cctgctgcaa cttacctccg ggatggtcca
     8701 gacccacgtc accacccccg gctccatacc gacgatctgc gacctggcgc gcacgtttgc
     8761 ccgggagatg ggggaggcta actgagctct agagctcgct gatcagcctc gactgtgcct
     8821 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     8881 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
     8941 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
     9001 aatagcaggc atgctgggga tgcggtgggc tctatggcat atggatctgc gatcgctccg
     9061 gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg
     9121 tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg
     9181 tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg
     9241 ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acagctgaag cttcgagggg
     9301 ctcgcatctc tccttcacgc gcccgccgcc ctacctgagg ccgccatcca cgccggttga
     9361 gtcgcgttct gccgcctccc gcctgtggtg cctcctgaac tgcgtccgcc gtctaggtaa
     9421 gtttaaagct caggtcgaga ccgggccttt gtccggcgct cccttggagc ctacctagac
     9481 tcagccggct ctccacgctt tgcctgaccc tgcttgctca actctacgtc tttgtttcgt
     9541 tttctgttct gcgccgttac agatccaagc tgtgaccggc gcctacgcta gcgctaccgg
     9601 tcgccaccat ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc
     9661 gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg
     9721 gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc
     9781 ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg
     9841 tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt
     9901 gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc
     9961 tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg
    10021 gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg
    10081 aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact
    10141 acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct
    10201 acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac
    10261 agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagtaa ggtaccatac
    10321 tcgtatagca tacattatac gaagttataa acccgctgat cagcctcgac tgtgccttct
    10381 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
    10441 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
    10501 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
    10561 agcaggcatg ctggggatgc ggtgggctct atgggtctcg ggaggtggtc ccactagtta
    10621 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
    10681 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
    10741 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
    10801 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
    10861 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
    10921 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
    10981 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
    11041 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
    11101 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
    11161 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
    11221 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
    11281 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
    11341 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
    11401 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
    11461 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
    11521 cctcactgat taagcattgg tagtagaaaa gatcaaagga tcttcttgag atcctttttt
    11581 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
    11641 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
    11701 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
    11761 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
    11821 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
    11881 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
    11941 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
    12001 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
    12061 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
    12121 gtgatgctcg tcaggg
//
