LOCUS       sequence-502972-       11791 bp    DNA     circular SYN 26-MAR-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11791)
  AUTHORS   Solomon DA, Kim JS, Cronin JC, Sibenaller Z, Ryken T, Rosenberg SA, 
            Ressom H, Jean W, Bigner D, Yan H, Samuels Y, Waldman T
  TITLE     Mutational inactivation of PTPRD in glioblastoma multiforme and 
            malignant melanoma.
  JOURNAL   Cancer Res. 2008 Dec 15. 68(24):10300-6.
  PUBMED    19074898
REFERENCE   2  (bases 1 to 11791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 26, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer Res.
            2008 Dec 15. 68(24):10300-6."
COMMENT     Sequence Label: pCDF1-PTPRD-WT
FEATURES             Location/Qualifiers
     source          1..11791
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        1347..1550
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1547..1571
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        6877..7088
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             7101..7369
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             7396..7995
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7396..7415)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7892..7912
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8002..8590
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8055..8075)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8473..8484)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    8927..9048
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8964..8983)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9018..9037
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9088..9223
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9256..9272)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9256..9272)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9269..9291)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9280..9296
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9304..9310,9311..9328,9329..9334))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9349..9370
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9487..9504)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9658..10246)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9738..9757)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10417..11208,11209..11277))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11040..11059
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11278..11382)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     11450..11468
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(11506..11528)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     11628..11647
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agcttgtgaa acttcgagga gtctctttgt tgaggacttt
      241 tgagttctcc cttgaggctc ccacagatac aataaatatt tgagattgaa ccctgtcgag
      301 tatctgtgta atctttttta cctgtgaggt ctcggaatcc gggccgagaa cttcgcagtt
      361 ggcgcccgaa cagggacttg attgagagtg attgaggaag tgaagctaga gcaatagaaa
      421 gctgttaagc agaactcctg ctgacctaaa tagggaagca gtagcagacg ctgctaacag
      481 tgagtatctc tagtgaagca gactcgagct cataatcaag tcattgttta aaggcccaga
      541 taaattacat ctggtgactc ttcgcggacc ttcaagccag gagattcgcc gagggacagt
      601 caacaaggta ggagagattc tacagcaaca tggggaatgg acaggggcga gattggaaaa
      661 tggccattaa gagatgtagt aatgttgctg taggagtagg ggggaagagt aaaaaatttg
      721 gagaagggaa tttcagatgg gccattagaa tggctaatgt atctacagga cgagaacctg
      781 gtgatatacc agagacttta gatcaactaa ggttggttat ttgcgattta caagaaagaa
      841 gagaaaaatt tggatctagc aaagaaattg atatggcaat tcctgcattg aggagaaatg
      901 gtaggcaatg tggcatgtct gaaaaagagg aggaatgatg aagtatctca gacttatttt
      961 ataagggaga tactgtgctg agttcttccc tttgaggaag gtatgtcata tcctagacat
     1021 agtctcaatt ttaaaagaag aggtaggata ggagggatgg ccccttatga attattagca
     1081 caacaagaat ccttaagaat acaagattat ttttctgcaa taccacaaaa attgcaagca
     1141 cagtggattt attataaaga tcaaaaagat aagaaatgga aaggaccaat gagagtagaa
     1201 tactggggac agggatcagt attattaaag gatgaagaga agggatattt tcttataatc
     1261 gatactagta ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
     1321 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
     1381 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
     1441 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
     1501 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
     1561 accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga agattctaga
     1621 gcccgggcgc gccggatcca gatcttaatg catgctcgag cggccgccag tgtgatggat
     1681 atctgcagaa ttcgcccttc accccggagg cagtgattcc aagctgctcg cgcacgctgc
     1741 tgccaagctg caggatggtg cacgtagcca ggctgctgct gctgctcctc actttcttcc
     1801 tccgcacgga tgctgagaca cctccaaggt ttacacgaac acccgttgat cagacagggg
     1861 tctctggcgg agttgcctct ttcatctgcc aagctacggg agacccaaga cctaaaattg
     1921 tctggaacaa aaaaggaaag aaagtcagca atcagagatt tgaggtaata gagtttgacg
     1981 atgggtctgg atcagttctc agaatacaac ccttacggac tccgagggat gaggccattt
     2041 atgaatgtgt ggcctcaaat aatgtgggag aaataagtgt atccaccaga ctcacagttt
     2101 tgcgggaaga tcaaattccc aggggcttcc ctaccattga catgggccca cagttgaagg
     2161 tggttgagcg tactcgcacg gccaccatgc tttgtgcagc cagtggtaat ccggatccag
     2221 aaatcacttg gtttaaagat ttcttacctg tggacacaag caacaacaat ggtcgtatta
     2281 agcagttacg atcagaatct attggtggta caccaataag aggagccctt cagattgagc
     2341 agagtgaaga gtctgaccaa ggaaaatatg agtgtgttgc caccaacagc gcgggcactc
     2401 gctattccgc tcctgccaat ttatatgtca gagagctgcg agaagttcgc cgtgtcccac
     2461 caagattctc tatcccaccc actaatcatg aaatcatgcc aggcggaagc gttaatatca
     2521 cctgtgtggc cgtggggtca ccaatgcctt atgtaaagtg gatgttgggg gcagaagatc
     2581 tgacacctga agatgatatg ccaataggaa gaaatgtgct agaactgaat gatgtaagac
     2641 agtcagcaaa ttacacctgt gttgctatgt caacactggg tgtcattgaa gcaatagcac
     2701 agatcactgt caaagcctta cccaaacctc caggaactcc tgtagtgacc gagagcacag
     2761 ctacaagcat cacactgacg tgggactctg ggaaccctga gcctgtttct tattacataa
     2821 ttcagcataa acctaaaaac tctgaggaac tttacaaaga aattgatggg gtggcgacca
     2881 cacgctacag tgtcgctgga ctaagtccct actcggatta tgaattcagg gttgttgctg
     2941 tcaataacat tgggcggggg cctcccagcg aacctgtgct aacacaaacc tcagagcaag
     3001 caccatccag tgccccgagg gatgtccagg cacgaatgtt gagttcgacc accattttgg
     3061 tacagtggaa ggaacctgaa gagccaaatg gacagatcca aggatataga gtttattata
     3121 caatggatcc cactcaacat gtcaacaact ggatgaaaca caatgtagct gacagccaaa
     3181 tcactactat tggcaactta gtgccccaga aaacatattc tgtcaaagtc ctggctttta
     3241 cctcaattgg agatggtccc ctttcaagtg acatacaagt catcactcag acaggagtac
     3301 cagggcagcc actaaacttc aaagcagaac ctgagtctga aacaagtatt ttgctctctt
     3361 ggacacctcc acgttcagat accattgcca actatgaact ggtctacaaa gatggggagc
     3421 atggagagga gcaacgaatt accattgagc cagggacatc atataggctg caaggactga
     3481 aaccaaacag cttatactat ttccgtctgg ctgcacgctc ccctcaaggc ctgggtgctt
     3541 ctactgcaga aatatcagct agaaccatgc agtcaatgtt tgcaaaaaat tttcatgtca
     3601 aagcagtaat gaagacttcc gtgttgctgt cttgggagat tccagagaat tataactccg
     3661 ccatgccttt caaaattctt tatgatgatg ggaaaatggt agaagaagtg gatggccgag
     3721 ccacacagaa gttaattgtc aacctgaagc ctgagaaatc atattcattt gtgctgacaa
     3781 atcgtggaaa cagtgctggt gggctgcagc acagggtcac ggcaaagact gcaccagatg
     3841 tattacgtac caagcctgcc ttcattggga agaccaactt ggatggcatg attactgtgc
     3901 aactgcctga agtacctgca aatgagaata taaaaggtta ctacataata attgtgcctt
     3961 tgaagaaatc tcgcgggaaa tttatcaagc catgggagag tccagatgaa atggaattag
     4021 atgagctgct taaggagata tctaggaagc gcagaagcat ccgttatggg agagaagttg
     4081 aattaaagcc atatattgcc gctcactttg atgtccttcc cactgagttc accctggggg
     4141 atgacaagca ttatggtgga tttacaaaca agcaactcca aagtggtcaa gaatatgtct
     4201 tctttgtgtt agcagtaatg gaacatgcag agtctaagat gtatgcaacc agcccttact
     4261 ccgaccccgt ggtgtcaatg gatctggatc cgcagccaat cacggatgaa gaagaaggct
     4321 tgatctgggt tgtaggtcct gtccttgcag tggtctttat catctgcatt gtcattgcta
     4381 ttcttcttta taaaagcagt aaacccgaca ggaagagggc agagtccgac tctagaaaaa
     4441 gcagcatacc gaacaataag gagatccctt cacaccaccc aacagaccct gtagaactga
     4501 ggcgccttaa ctttcaaaca ccgggtatgg ctagccatcc tccaataccc atcttggaac
     4561 ttgcagacca cattgaaaga ttgaaagcaa atgacaactt gaagttttcc caggaatatg
     4621 agtcaattga ccctggccag cagttcactt gggaacattc aaacttggaa gtaaacaaac
     4681 caaagaatag atacgcgaat gtaatcgcat atgatcattc ccgggttctc ctatcagcta
     4741 tagaagggat cccaggaagt gactatgtga atgccaacta catagatggg tataggaagc
     4801 aaaatgccta tattgcaaca cagggatctc tccccgaaac atttggggac ttttggagaa
     4861 tgatatggga acaacggagt gccacagttg tcatgatgac aaaactagaa gaaagatcaa
     4921 gggtgaagtg tgaccagtat tggcctagca gaggcacaga aacccacgga ctcgttcaag
     4981 taacgctgct tgatactgtg gagctggcca catattgtgt tcgaacattt gcactttaca
     5041 agaatggttc aagtgagaag agagaagtga gacaattcca gttcaccgcc tggcctgatc
     5101 atggtgttcc agaacaccct acaccttttc tagctttctt acgtagagtc aaaacctgta
     5161 accctcccga tgctggtccg atggttgtgc actgcagtgc gggagttggc cggactggtt
     5221 gcttcatcgt catagatgcc atgttagaaa gaataaagca tgaaaaaact gtagatattt
     5281 atggccatgt aactttaatg agagcccaga ggaactatat ggttcaaaca gaagaccaat
     5341 acatctttat ccatgatgca ctgttagaag cagtgacttg tggaaatacc gaagtgccag
     5401 ctagaaactt gtatgcctac attcagaagc tgacacaaat agaaacggga gagaatgtca
     5461 caggaatgga gctcgaattt aagcgtctag ccagctcaaa agctcacacc tcaaggttta
     5521 tcagtgccaa tcttccatgt aataaattca aaaatcgcct tgttaatatt atgccatatg
     5581 aatccacaag ggtatgcctg cagcctatcc gtggagtaga aggatctgat tacatcaatg
     5641 ccagttttat tgatggatac agacaacaga aagcctacat cgctacccag gggcccttgg
     5701 cagagaccac tgaagacttc tggcggatgc tctgggaaca caattccacc atagttgtga
     5761 tgctcaccaa gctgcgtgaa atgggcagag agaaatgtca ccaatactgg ccagcagaac
     5821 ggtctgcaag ataccagtac tttgttgtag atcccatggc tgagtacaac atgccacagt
     5881 atatcctaag ggaattcaag gtcacagatg ccagggacgg ccagtcccga acagtaaggc
     5941 agttccagtt cactgactgg ccagagcaag gagtgccaaa gtccggagaa ggatttattg
     6001 acttcatcgg ccaagtccat aaaacaaaag aacagtttgg ccaagatgga cccatttcag
     6061 tccattgcag cgcgggcgtt ggaagaactg gagtcttcat aacgctaagc attgttttgg
     6121 aaagaatgag atatgaagga gttgtagata tcttccagac tgtcaaaatg ttaagaacac
     6181 aacgaccagc tatggtacag acagaggatc aatatcagtt ttcctatcgt gccgcactag
     6241 agtacctggg cagctttgac cactatgcaa cgtagaaacc cctgacccat tctggatttt
     6301 tactacaggc ccttcaatat ccatggagtc tcttctgagc catacagggc acttgagaag
     6361 tccttcttaa cttctagcta acaactactt agtgggacta ttacacacaa aacaaattaa
     6421 aaacaaatta ttccaggtgg accaagaatt cacagtttaa taatctaacc tgaaaaataa
     6481 tgaagagaat cctgactgat gaggcatctg aaggatttta tttacagata cctcaaggat
     6541 tcaaaatcaa gggaagaaga aatcacagca aagaaccacc atcttattca ggattgcttg
     6601 aaaggtgcaa ggagaaattg ccagaatgct gcagttcagc acaagatatg tgctggtgtg
     6661 gcttctcaca atgaaacgga ctctgctttg acatcgcccc ttcccaccat actgctcata
     6721 ataacatttt aggggccaag gggaataata taaagggcga attccagcac actggcggcc
     6781 gttactagtg gatccgagct cggtaccaag cttgatgcat aatttaaatg aattcgcggc
     6841 cgcgaaggat ctgcgatcgc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag
     6901 tccccgagaa gttgggggga ggggtcggca attgaacggg tgcctagaga aggtggcgcg
     6961 gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag
     7021 aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca
     7081 gaacacagct gaagcttcga ggggctcgca tctctccttc acgcgcccgc cgccctacct
     7141 gaggccgcca tccacgccgg ttgagtcgcg ttctgccgcc tcccgcctgt ggtgcctcct
     7201 gaactgcgtc cgccgtctag gtaagtttaa agctcaggtc gagaccgggc ctttgtccgg
     7261 cgctcccttg gagcctacct agactcagcc ggctctccac gctttgcctg accctgcttg
     7321 ctcaactcta cgtctttgtt tcgttttctg ttctgcgccg ttacagatcc aagctgtgac
     7381 cggcgcctac gctagatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
     7441 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac
     7501 accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg
     7561 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc
     7621 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg
     7681 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg
     7741 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag
     7801 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg
     7861 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc
     7921 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc
     7981 aagcccggtg cctgagtcga caatcaacct ctggattaca aaatttgtga aagattgact
     8041 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     8101 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     8161 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     8221 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     8281 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     8341 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     8401 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     8461 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     8521 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     8581 gcctccccgc ctggtaccga tgacagagtt agaagatcgc ttcaggaagc tatttggcac
     8641 gacttctaca acgggagaca gcacagtaga ttctgaagat gaacctccta aaaaagaaaa
     8701 aagggtggac tgggatgagt attggaaccc tgaaatcgat agcttccagt gctttgtgaa
     8761 acttcgagga gtctctttgt tgaggacttt tgagttctcc cttgaggctc ccacagatac
     8821 aataaatatt tgagattgaa ccctgtcgag tatctgtgta atctttttta cctgtgaggt
     8881 ctcggaatcc gggccgagaa cttcgcagcg agctcattgt accgcgaact tgtttattgc
     8941 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     9001 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct
     9061 ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     9121 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     9181 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagactt ttgcagagac
     9241 caaattcgta atcatgtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     9301 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     9361 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     9421 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     9481 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     9541 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     9601 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     9661 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     9721 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     9781 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     9841 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     9901 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     9961 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
    10021 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
    10081 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
    10141 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
    10201 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
    10261 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
    10321 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
    10381 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
    10441 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
    10501 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
    10561 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
    10621 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
    10681 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
    10741 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
    10801 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
    10861 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
    10921 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
    10981 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
    11041 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
    11101 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
    11161 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
    11221 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
    11281 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
    11341 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
    11401 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
    11461 cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
    11521 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
    11581 gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca
    11641 gattgtactg agagtgcacc atatgccaag tacgccccct attgacgtca atgacggtaa
    11701 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
    11761 catctacgta ttagtcatcg ctattaccat g
//
