LOCUS       sequence-503001-        6953 bp    DNA     circular SYN 16-MAR-2026
DEFINITION  GFP-tagged polyglycine protein expressed from the antisense 
            transcript of RILPL1. This vectors uses 100 GGN optimized codons..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6953)
  TITLE     pAAV uGIP polyGly (exon 4 Cter) GFP
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 16, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAV asRILpolyGly GFP (OPDM type 4)
FEATURES             Location/Qualifiers
     source          1..6953
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     enhancer        206..509
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        510..713
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     664..684
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     710..734
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          862..1240
                     /label=chimeric intron
                     /note="fusion between human cytomegalovirus intron A and 
                     human beta-globin intron 2"
     CDS             join(2900..2902,2903..3616)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(2900..2902,2903..3613)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(3203..3222)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3550..3571
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3659..3685
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    3700..4176
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3899..3918)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   4216..4356
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4216..4345
                     /label=AAV2 ITR
     rep_origin      4431..4886
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4518..4537)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4728..4749
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4903..4922)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5022..5044
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5082..5100)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5168..5272
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5273..5341,5342..6133)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5491..5510)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6304..6892
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6793..6812
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtctagtt attaatagta atcaattacg gggtcattag
      181 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
      241 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
      301 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
      361 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
      421 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
      481 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
      541 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
      601 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
      661 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag
      721 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc
      781 gggaccgatc cagcctccgc ggattcgaat cccggccggg aacggtgcat tggaacgcgg
      841 attccccgtg ccaagagtga cgtaagtacc gcctatagag tctataggcc cacaaaaaat
      901 gctttcttct tttaatatac ttttttgttt atcttatttc taatactttc cctaatctct
      961 ttctttcagg gcaataatga tacaatgtat catgcctctt tgcaccattc taaagaataa
     1021 cagtgataat ttctgggtta aggcaatagc aatatttctg catataaata tttctgcata
     1081 taaattgtaa ctgatgtaag aggtttcata ttgctaatag cagctacaat ccagctacca
     1141 ttctgctttt attttatggt tgggataagg ctggattatt ctgagtccaa gctaggccct
     1201 tttgctaatc atgttcatac ctcttatctt cctcccacag ctcctgggca acgtgctggt
     1261 ctgtgtgctg gcccatcact ttggcaaaga attgggattc gaacatcgat tgaattcaaa
     1321 gcttgccatg cgatcaatcc tgtcccgacg gcgacgctca tcatctagaa gaagcagcag
     1381 ttcatctggg gctacctggt ggagactgac ctcaacctcc cggatctcta ggacacgcac
     1441 cacactgggc atgcggagag ctatggcctc tcagccatgc tggagcatga ccagaagcaa
     1501 ctcctggccc acaagcgacg ccatgagcta cacctccatg acagtgagct ccgccacctt
     1561 cttttctagc gccgactctg ccgcaagcgc cgatcccagg tcctctagca tggccacact
     1621 gctggcctgt ccccctccaa gaaagctggt gcagctgcca aatctgcctc tgtccagggc
     1681 aggaccagcc ggcccctctc tgggccgggg cagagccagg agcgccggag caggagcaag
     1741 ggcacagagg gccgccggcg gcggccgggg cagaggccac ggcaggcgcc ggggcgtgtg
     1801 cggcccaggc gtgtgggccc ccggctccgc cagatcttgt agcagggtgg gaggcggcgg
     1861 aggaggaggg ggagggggtg gaggaggggg agggggagga ggagggggag gtggaggcgg
     1921 gggaggagga ggaggggggg gaggaggggg aggaggtgga gggggaggag gggggggagg
     1981 gggaggcggg ggaggagggg gggggggagg aggagggggg ggcggaggcg gggggggcgg
     2041 gggaggagga gggggcgggg gagggggggg ggggggcgga ggagggggtg gagggggagg
     2101 aggaggcgga ggcgggggag ggggaggcgg cgggggaggc ggcggcggct ctggcgaggg
     2161 agcaagagtg agaaggcctg cagcaccacc taagctgggc agcgagctgc ggagcatgga
     2221 gccaggaaga gcacccaggg gcggcgccgg cctgcgcggc ggcagagcaa ggggccgccg
     2281 gggccgcgca gcaaggggct cctctggcaa gctgtggaga aggcgcctgc ggggcagagt
     2341 gatcgcatcc cctcagggcg gcagagcacc taggccaaac cccggcctgg cccgcggcgc
     2401 cccagccttc cggcggcccg gagcaccaag gggcacccca ccctccgccg actctgccgc
     2461 ctgcacacca ggacctccac tgcagccacc tgatcctagg cgcccttctc cagtgccccg
     2521 cccacccctg cctcggccag ccctgacccc tccaaccaca cggccccctg gcggcgtgca
     2581 ggcagcacca ggagagggag caggaggagc cggcctggcc ggccggtttg ccgcccctgg
     2641 ctccggccca ggcctgggcg agggatggag aggacacgag gaggtgcctg gcctgggcct
     2701 gatcccagga agagagaggg gaccaccacc tgcccggaga cacagcgccc agaccaggcc
     2761 acccggaggc ctggcagagg caccaggcga gggccctaca tgtcgcggca tgctggtgcc
     2821 ccgctcccct cgggcacggc tgcctgaacc acacccctcc ctggctcctt gcgacatgaa
     2881 tgctacatca cagggatccg tcagcaaagg cgaggagctt ttcaccgggg ttgttcccat
     2941 cctcgtcgag ctcgacggcg acgtaaacgg ccacaaattc agcgtttccg gcgagggcga
     3001 gggcgatgcc acctacggca agctcaccct caagttcatc tgcaccaccg gcaagctccc
     3061 cgtcccctgg cccaccctcg tcaccaccct cacctacggc gtccagtgct tcagccgcta
     3121 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
     3181 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
     3241 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
     3301 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc
     3361 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg
     3421 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
     3481 gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa
     3541 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
     3601 cgagctgtac aagtgataag aattctgcag atatccagca cagtggcggc cgctcgagta
     3661 cccatacgat gttccagatt acgctaagta aagatctacg ggtggcatcc ctgtgacccc
     3721 tccccagtgc ctctcctggc cctggaagtt gccactccag tgcccaccag ccttgtccta
     3781 ataaaattaa gttgcatcat tttgtctgac taggtgtcct tctataatat tatggggtgg
     3841 aggggggtgg tatggagcaa ggggcaagtt gggaagacaa cctgtagggc ctgcggggtc
     3901 tattgggaac caagctggag tgcagtggca caatcttggc tcactgcaat ctccgcctcc
     3961 tgggttcaag cgattctcct gcctcagcct cccgagttgt tgggattcca ggcatgcatg
     4021 accaggctca gctaattttt gtttttttgg tagagacggg gtttcaccat attggccagg
     4081 ctggtctcca actcctaatc tcaggtgatc tacccacctt ggcctcccaa attgctggga
     4141 ttacaggcgt gaaccactgc tcccttccct gtccttctga ttttgtaggt aaccacgtgc
     4201 ggaccgagcg gccgcaggaa cccctagtga tggagttggc cactccctct ctgcgcgctc
     4261 gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg
     4321 cctcagtgag cgagcgagcg cgcagctgcc tgcaggggcg cctgatgcgg tattttctcc
     4381 ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag caaccatagt acgcgccctg
     4441 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     4501 cagcgcctta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     4561 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     4621 gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc catcgccctg
     4681 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     4741 ccaaactgga acaacactca actctatctc gggctattct tttgatttat aagggatttt
     4801 gccgatttcg gtctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     4861 taacaaaata ttaacgttta caattttatg gtgcactctc agtacaatct gctctgatgc
     4921 cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
     4981 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
     5041 gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt
     5101 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     5161 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     5221 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     5281 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     5341 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     5401 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     5461 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     5521 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     5581 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     5641 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     5701 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     5761 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     5821 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     5881 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     5941 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     6001 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     6061 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     6121 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     6181 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     6241 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     6301 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     6361 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     6421 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     6481 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     6541 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     6601 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     6661 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     6721 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     6781 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     6841 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     6901 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgt
//
