LOCUS       sequence-503018-        7562 bp    DNA     circular SYN 16-MAR-2026
DEFINITION  Myc-HECT(TRIP12); the HECT domain (AA 1651-2040) of TRIP12 with an 
            N-terminal Myc-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7562)
  AUTHORS   Ibrahim M, Roos WP, Schwartz JC, Khan MM, Al-Rahahleh RQ, Beers LA, 
            Pearson CR, Langham KT, Boyang L, Clark J, Hayat F, Fang Q, Koczor 
            CA, Migaud ME, Sobol RW
  TITLE     Replication-associated base excision repair/single-strand break 
            repair regulates PARG inhibitor response via the PRMT1/PRMT5/ATR 
            axis.
  JOURNAL   NAR Cancer. 2025 Dec 31;7(4):zcaf057. doi: 10.1093/narcan/zcaf057. 
            eCollection 2025 Dec.
  PUBMED    41480640
REFERENCE   2  (bases 1 to 7562)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 16, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "NAR 
            Cancer."; date: "2025-12-31"; volume: "7(4):zcaf057. doi"; pages: " 
            10.1093/narcan/zcaf057. eCollection 2025 Dec"
COMMENT     Sequence Label: pENTR-Myc-HECT(TRIP12)
FEATURES             Location/Qualifiers
     source          1..7562
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             692..721
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    complement(5687..5786)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(5803..5822)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5804..5822)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5827..5843)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5827..5843)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5945..5964)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             5956..6765
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(6023..6042)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      6912..7500
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7401..7420
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc gcggccgccc ccttcaccat ggaacaaaaa ctcatctcag aagaggatct
      721 ggcagcatcc aaccggccta ataacaatcc aggggggtca ctgcgacgtt cacagaggaa
      781 cactgccggg gcccaaccac aagacgactc aataggagga aggtcacatt tagggcaggc
      841 aaaacataag ggatatagcc ctcctgagag tagaaaatct aattctaagg cacccaaagt
      901 gcagtctaat actacttctg aactgtcaag gggacacctt tctaaaagaa gctgcagttc
      961 atcatctgct gtgatagttc cacaaccaga ggatccagac agagccaata cttcagaaag
     1021 acaaaaaacg gggcaggtgc ctaagaaaga caattctcga ggagtgaagc gcagtgctag
     1081 tccagactac aacaggacca attctcctag ctctgcaaaa aaaccaaaag cacttcagca
     1141 tactgaatct ccctcagaaa caaataagcc acatagtaag tcaaagaaga gacatttaga
     1201 ccaggagcaa caactgaaat ctgcacaatc accatcaaca agcaaggctc ataccaggaa
     1261 gagtggggcc actggcggtt cacggagtca gaaaagaaaa aggacagaga gttcttgtgt
     1321 aaagagtggc tccgggtctg aatcaactgg tgcagaagag agatctgcga aacctaccaa
     1381 gctggcttca aaatcagcca cctcagccaa agctgggtgt agcaccatca ctgattcttc
     1441 ttctgctgcc tctacttcct cctcgtcttc tgctgtagcc tcggcctcct ccactgtacc
     1501 accaggtgcc agagtgaaac aaggaaaaga tcagaacaag gccaggcgtt cccgttcagc
     1561 gtccagtccc agccccagaa gaagtagcag ggaaaaggaa cagagtaaaa ctggtggctc
     1621 ttcaaaattt gattgggctg ctcgtttcag ccctaaagtt agccttccta aaacaaaact
     1681 gtctcttcca gggtcttcta agtcagagac atcaaaacct ggaccttctg gattacaggc
     1741 caaattagca agtttaagaa aatctacgaa gaaacgcagt gagtctccac ctgctgagct
     1801 ccccagtttg aggcggagca cacgccaaaa gaccacgggc tcctgtgcta gtaccagtcg
     1861 gcgaggctct ggcctgggca aaagaggagc agctgaagct cgtcgacagg agaaaatggc
     1921 agaccctgaa agcaaccagg aggcagtaaa ttcttcagct gctcggacag atgaagctcc
     1981 ccaaggagct gcagcctcta gttctgttgc aggggctgtt ggcatgacca cctctgggga
     2041 gagtgaatca gatgattccg agatgggacg tttgcaagct ttgttagagg caaggggtct
     2101 tccccctcac ctatttggtc ctcttggtcc tcggatgtca cagcttttcc atagaacaat
     2161 tggaagtgga gctagttcta aggcccagca gctactacaa ggattgcaag ccagtgatga
     2221 aagtcaacag cttcaggcag ttattgagat gtgtcagtta ctggtcatgg gaaatgagga
     2281 gacactggga gggtttcctg tcaagagtgt tgttccagct ttgattacgt tacttcagat
     2341 ggagcacaat tttgatatta tgaaccatgc ttgtcgagcc ttaacataca tgatggaagc
     2401 acttcctcga tcttctgctg ttgtagtaga tgctattcct gtctttttag aaaagctgca
     2461 agttattcag tgtattgatg tggcagagca ggccttgact gccttggaga tgttgtcacg
     2521 gagacatagt aaagccattc tacaggcggg tggtttggca gactgcttgc tgtacctaga
     2581 attcttcagc ataaatgccc aaagaaatgc attagcaatt gcagctaatt gctgccagag
     2641 tatcacgcca gatgaatttc attttgtggc agattcactc ccattgctaa cccaaaggct
     2701 aacacatcag gataaaaagt cagtagaaag cacttgcctt tgttttgcac gcctagtgga
     2761 caacttccag catgaggaga atttactcca gcaggttgct tccaaagatc tgcttacaaa
     2821 tgttcaacag ctgttggtag tgactccacc cattttaagt tctgggatgt ttataatggt
     2881 ggttcgcatg ttttctctga tgtgttccaa ctgtccaact ttagctgttc aacttatgaa
     2941 acaaaacatt gcagaaacgc ttcactttct cctgtgtggt gcctccaatg gaagttgtca
     3001 ggaacagatt gatcttgttc cacgaagccc tcaagagttg tatgaactga catctctgat
     3061 ttgtgaactt atgccatgtt taccaaaaga aggcattttt gcagttgata ccatgttgaa
     3121 gaagggaaat gcacagaaca cagatggtgc gatatggcag tggcgtgatg atcggggcct
     3181 ctggcatcca tataacagga ttgacagccg gatcattgag caaatcaatg aggacacggg
     3241 aacagcacgt gccattcaga gaaaacctaa cccgttagcc aatagtaaca ctagtggata
     3301 ttcagagtca aagaaggatg atgctcgagc acagcttatg aaagaggatc cggaactggc
     3361 taagtctttt attaagacat tatttggtgt tctttatgaa gtgtatagtt cctcagcagg
     3421 acctgcggtc agacataagt gccttagagc aattcttagg ataatttatt ttgcggatgc
     3481 tgaacttctg aaggatgttc tgaaaaatca tgctgtttca agtcacattg cttccatgct
     3541 gtcaagccaa gacctgaaga tagtagtggg agcacttcag atggcagaaa ttttaatgca
     3601 gaagttacct gatattttta gtgtttactt cagaagagaa ggtgtaatgc atcaagtaaa
     3661 acacttagca gaatcagagt ctttgttgac aagtccacca aaggcatgta cgaatggatc
     3721 gggatcaatg ggatccacaa cttcagtcag cagtgggaca gccacagctg ccactcatgc
     3781 tgcagctgac ttgggatcac ccagcttgca gcacagcagg gatgattctt tagatctcag
     3841 ccctcaaggt cgattaagtg atgttctaaa gagaaaacga ctgccaaaac gagggccaag
     3901 aaggccaaag tactcacctc caagagatga tgacaaagta gacaatcaag ctaaaagccc
     3961 caccactact cagtcaccta aatcttcttt cctggcaagc ttgaatccaa aaacatgggg
     4021 aaggttaagt acacagtcca acagcaacaa cattgagcca gcacggactg cgggaggtag
     4081 tggccttgcc agggctgcct caaaggatac catctccaat aatagagaaa aaattaaagg
     4141 ttggattaag gagcaggcac ataaatttgt agaacgttat ttcagttctg agaatatgga
     4201 tggaagcaac cctgcattga atgtccttca gagactttgt gctgcaaccg aacaactcaa
     4261 cctccaggtg gatggtggag ctgagtgcct tgtagaaatc cgtagcatag tctcagagtc
     4321 agatgtttca tcatttgaaa tccaacatag tggatttgtg aagcagctgt tgctttattt
     4381 gacatctaaa agtgaaaagg atgctgtgag cagagagatc agattaaagc gatttcttca
     4441 tgtatttttt tcttctccac ttcctggaga agagcccatt ggaagagtgg aaccagtggg
     4501 taatgcacct ttgttggcat tagttcacaa gatgaacaac tgcctcagcc agatggaaca
     4561 atttccagtc aaagtacatg atttccctag tggaaatggg acaggaggca gcttttctct
     4621 caacagagga tcacaggctt taaaattttt caacacacat caattaaaat gccagttaca
     4681 aaggcatcca gactgtgcaa atgtgaagca gtggaagggt ggacctgtca agattgaccc
     4741 tctggctttg gtacaagcca tcgagagata ccttgtagtt agagggtatg gaagagtaag
     4801 agaagatgat gaagacagcg atgacgatgg atcagatgag gaaatagatg agtctctggc
     4861 tgctcagttc ctaaattcag gaaatgtaag acacaggctg cagttttata ttggagaaca
     4921 tttgctgccg tataacatga ctgtgtatca ggcagtacgg cagtttagta tacaggctga
     4981 agatgaaaga gaatccacag atgatgagag caatcctcta ggcagagctg gtatttggac
     5041 aaagactcat acaatatggt ataaacctgt gagagaggat gaagaaagta ataaagattg
     5101 tgttggtggt aaaagaggaa gagcccaaac agctccaacg aaaacttccc ctagaaatgc
     5161 aaaaaagcat gatgagttat ggcacgatgg agtgtgccca tcagtatcaa atcctttaga
     5221 agtttacctc attcccacac cacctgaaaa tataacattt gaagacccgt cattagatgt
     5281 gatccttctt ttaagagttt tacatgctat cagtcgatac tggtattact tgtatgataa
     5341 tgcaatgtgc aaggaaatta ttccaactag tgaatttatt aacagtaagt taacagcaaa
     5401 agcaaatagg caacttcaag atcctttagt aatcatgaca ggaaacatcc caacatggct
     5461 tactgagcta ggaaaaacct gcccattttt ctttcctttt gatacccggc aaatgctttt
     5521 ttatgtaact gcatttgatc gggaccgagc aatgcaaaga ttacttgata ccaacccaga
     5581 aatcaaccag tctgattctc aagatagcag agttgcacct agattggata gaaaaaaacg
     5641 tactgtgaac cgagaggagc tgctgaaaca ggcgtgaggc gcgccgaccc agctttcttg
     5701 tacaaagttg gcattataag aaagcattgc ttatcaattt gttgcaacga acaggtcact
     5761 atcagtcaaa ataaaatcat tatttgccat ccagctgata tcccctatag tgagtcgtat
     5821 tacatggtca tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct ctgatgttac
     5881 attgcacaag ataaaaatat atcatcatga acaataaaac tgtctgctta cataaacagt
     5941 aatacaaggg gtgttatgag ccatattcaa cgggaaacgt cgaggccgcg attaaattcc
     6001 aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt
     6061 gcgacaatct atcgcttgta tgggaagccc gatgcgccag agttgtttct gaaacatggc
     6121 aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg gctgacggaa
     6181 tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc atggttactc
     6241 accactgcga tccccggaaa aacagcattc caggtattag aagaatatcc tgattcaggt
     6301 gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt
     6361 aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat
     6421 aacggtttgg ttgatgcgag tgattttgat gacgagcgta atggctggcc tgttgaacaa
     6481 gtctggaaag aaatgcataa acttttgcca ttctcaccgg attcagtcgt cactcatggt
     6541 gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt
     6601 ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt
     6661 gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat
     6721 atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaatcaga attggttaat
     6781 tggttgtaac actggcagag cattacgctg acttgacggg acggcgcaag ctcatgacca
     6841 aaatccctta acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc
     6901 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     6961 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     7021 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     7081 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     7141 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     7201 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     7261 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     7321 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     7381 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     7441 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     7501 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     7561 tt
//
