LOCUS       sequence-503023-       16861 bp    DNA     circular SYN 16-MAR-2026
DEFINITION  CRISPR-Cas9 vector containing two 20 nt protospacer sequences to 
            target the gene wA in Aspergillus nidulans. pyrG marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16861)
  TITLE     Mortensen Lab plasmids
REFERENCE   2  (bases 1 to 16861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 16, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pDIV1064
FEATURES             Location/Qualifiers
     source          1..16861
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_RNA        complement(3314..3389)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_RNA        complement(3485..3560)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     rep_origin      4393..9640
                     /label=AMA1
                     /note="replication origin from Aspergillus nidulans 
                     (Aleksenko and Clutterbuck, 1996)"
     protein_bind    complement(9953..9986)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(10193..10212)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(10218..10237)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        10279..10370
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10371..10439,10440..11231)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10589..10608)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11353..11941
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11842..11861
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12095..12112
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     12211..12227
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    12697..12730
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             complement(14846..14866)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(join(14870..16861,1..2112))
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(join(14870..16861,1..2112))
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
ORIGIN
        1 gaacgtcagg gaatcgtcgt gaataagctg cataaaattg cggttggcga agccgtccga
       61 cttaaggaaa tccaagatag tcttaccaga ttgcttatct cgaatgccgt tgatcagctt
      121 ccggctaagg cgaccccatc cggtgtatcg acgccgcttc agctgcttca tcaccttatc
      181 gtcgaacaaa tgggcatatg tcttgagccg ttcctcgatc atttcgcggt cctcaaaaag
      241 tgtcaacgtg aggacaatat cttccaagat gtcctcattt tcctcgttat cgaggaaatc
      301 cttgtcctta atgatcttga gcaggtcgtg gtaggtaccg agggaagcgt taaagcgatc
      361 ttccacgccg ctgatctcga cggagtcgaa acattcaatc ttcttgaaat aatcctcctt
      421 cagctgctta acagtcactt tacgattggt cttaaaaagc aaatcgacga tagccttctt
      481 ttgttcccct gagaggaagg ctggcttccg cattccctcc gtcacgtact tgaccttagt
      541 cagttcgtta tacacggtga aatactcgta gagcagcgaa tgctttggca ggaccttttc
      601 attcggaagg ttcttatcaa aattggtcat ccgctcaatg aagctctgcg cggatgcacc
      661 cttgtccacg acttcctcga agttccacgg agtgatggtt tcctcgctct ttcgggtcat
      721 ccaagcaaat cgggaattcc cacgggccaa aggtcccaca tagtagggga ttcggaatgt
      781 aagaatcttt tcgatcttct cacggttgtc cttcagaaat gggtagaaat cttcctggcg
      841 tcgaaggatg gcgtggagct cgcccagatg aatctggtgc gggatggagc cgttgtcgaa
      901 cgttcgttgc ttacgaagca aatcttcgcg attcagcttg acgagcagtt cctcagtgcc
      961 gtccatcttc tccaaaatcg gcttgataaa cttgtagaat tcctcctggc tcgcaccgcc
     1021 atcaatatac cctgcgtatc cgttcttgga ttggtcaaag aagatttcct tatacttctc
     1081 aggcaattgc tgacgcacga gggccttaag caaggtgagg tcctgatggt gctcatcata
     1141 ccgcttgatc atactagctg acaggggggc cttagtaatc tcggtgttga cccgcaaaat
     1201 gtcactgagc aggattgcat ctgagagatt cttagcggcc aggaaaagat cagcgtactg
     1261 gtcaccgatt tgggcaagca agttgtcgag atcgtcatca tacgtatcct tagacaactg
     1321 gagcttcgca tcctctgcca ggtcaaaatt actcttgaag ttaggagtca gaccaagtga
     1381 caacgcaatc aaattaccga agaggccgtt cttcttttcg ccggggagtt gtgcgataag
     1441 gttctccaaa cgccgcgact tagacaggcg cgccgaaagg atagccttgg cgtccacgcc
     1501 agatgcatta atagggtttt cctcaaacag ctgattgtag gtttggacca actggatgaa
     1561 gagcttatca acgtcagaat tatcggggtt caggtcaccc tcgataagaa aatggccgcg
     1621 gaacttaatc atgtgcgcga gtgccaggta gataagtcgc aaatcagcct tgtccgtgct
     1681 atcgaccagc ttcttacgaa gatgatagat agttgggtac ttttcgtgat aggcaacctc
     1741 atccacaatg ttaccaaaga tcggatggcg ctcgtgcttc ttatcctcct cgaccaagaa
     1801 actttcttcg agtcgatgaa agaatgagtc atcaacctta gccatttcgt tgctgaaaat
     1861 ctcctggagg tagcagatgc gattcttgcg tcgtgtataa cgccggcgag ccgttcgctt
     1921 cagtcgagta gcttcggcgg tctccccact atcgaagagc agggctccaa tgagattctt
     1981 cttgattgag tgacggtcgg tgtttcccag gaccttaaac ttcttggatg gcaccttgta
     2041 ttcgtccgta atcacagccc aaccaaccga gttcgttcca atgtccagcc cgatgctata
     2101 cttcttgtcc atggtgaagg ttgtgttatg ttttgtggaa tataaaaggg agaagtgctc
     2161 agatactacg gctagtaatg cgtagaggtg ctgagcagag aagttagcag gagatggatg
     2221 aaagaaggat ggaggagaga agcgagggaa gggaagatat acgtactgaa gcggacaagg
     2281 agaggaaggg aaagaaaatt ttgtgggagg gggaggggaa atccttttat ggaggggcaa
     2341 gcgaggacga cgactaagcg ggcgggctca aacccttggc tggtagatat ctgtcattgg
     2401 atatggaggg gtaacccgcc ccactcccga tgcaaattct tctcctagac tcccaccttg
     2461 ggcgtcgagc ctttacaaca ccttccggaa cggcagccca gcgcgactct ttccttggtt
     2521 ctgctccacc ccatctcgtt ttcatggctt aggggcagaa attttttttt gtttagcttt
     2581 tctttacttg ctatcgcctc tacggtggat tctcggatcg aaaaagctgg gcatcaacgc
     2641 gttgatggtc gcatggcggc ttttatcact ctgtactttg cagcggacaa ccccgcggct
     2701 gtacgagttt gccgcctttt aatttgtctg cgaccagcct gcgatggagc gagcgagtgg
     2761 cgttgcttga tccacagcgt caagatcaac atacgtagca agatagaccg caagaaaatg
     2821 atgctcggaa tccatattga ctcacgaaaa tttctttctt tttgcccccc tcatctgttg
     2881 cctgggaggt gagtcaaatt gcccctcact aaacgctcct ttgcttttcc cgaacttggc
     2941 ccgttcgaga gcatgatcag cacaaaggct taacttgggc ggtgattctg ctgtctcggc
     3001 tgaggtctta ataccctgag aagatagatg tgaatgtgtg catagacatg caggtcaatg
     3061 agcaatttaa tgtgattcta tgaataaagt tgacggcaat ctcaatctac aaatagtcta
     3121 cttggaagta aaatgaacta gtcatgtaaa aattatattc ttcagaatct aaaacaagag
     3181 agaatttgag caaactctga tcgcctgatt acaccaacat caagtgtaaa aattgacatg
     3241 catcatccgt gaatcgaaca cgggcctcat cgatggcaac gatgaattct accactagac
     3301 caatgatgca aaagcaccga ctcggtgcca ctttttcaag ttgataacgg actagcctta
     3361 ttttaacttg ctatttctag ctctaaaaca ttgatatatg catgtcaact gcatcatccg
     3421 tgaatcgaac acgggcctca tcgatggcaa cgatgaattc taccactaga ccaatgatgc
     3481 aaaagcaccg actcggtgcc actttttcaa gttgataacg gactagcctt attttaactt
     3541 gctatttcta gctctaaaac aagaggccga tcgagttgaa tgcatcatcc gtgaatcgaa
     3601 cacgggcctc atcgatggca acgatgaatt ctaccactag accaatgatg cgctgttagc
     3661 aaaatgttct gacttgcagt tgagaggttt tcccaaaggt agggaggggg atgacatacg
     3721 tagagtcgct tgatttccaa aatgcatggg atttcggggc cttttatatt tgcgggcagg
     3781 cgctagcgag tgcgctaacc tcaagcgaaa attttcatgt ttgctcggtt tgttctgtgg
     3841 ggctacctgt ggtaccaatc agagttagtt gaagattttc cgctccactg gccagactgt
     3901 ccccgagctc accggtgaat gggctccagc gtaaaatttc aacatcaggg atggtctccc
     3961 aaatatttgc ttctcagtgg ctgttgagcg acttcatgag gatgcgatga caaacagtaa
     4021 ctcactgata tcctgattgg atttgaagcc aagcgttagg agaacctcct acaaagtact
     4081 tgctataggt ggtcctgtca accgagcatc tatgtgatca ttaaaccctc agcggaaaca
     4141 gctatgacca tgagatcttc ccgggcccag tatatgttcc gcagatgact ggagctctgc
     4201 catacgtgcc ctctcaagca ccatttgttc catctacaga gactagtcac caactagtct
     4261 atcaagactc acagggtaca ttgctgagac caactgacca gaggcagggt agcggattga
     4321 cggctccatc tccttcactt acaaggtcta ttgaaagccc tttagcatca ccaagcggag
     4381 aatagattgt taagcttatt ttttgtatac tgttttgtga tagcacgaag tttttccacg
     4441 gtatcttgtt aaaaatatat atttgtggcg ggcttaccta catcaaatta ataagagact
     4501 aattataaac taaacacaca agcaagctac tttagggtaa aagtttataa atgcttttga
     4561 cgtataaacg ttgcttgtat ttattattac aattaaaggt ggatagaaaa cctagagact
     4621 agttagaaac taatctcagg tttgcgttaa actaaatcag agcccgagag gttaacagaa
     4681 cctagaaggg gactagatat ccgggtaggg aaacaaaaaa aaaaaacaag acagccacat
     4741 attagggaga ctagttagaa gctagttcca ggactaggaa aataaaagac aatgatacca
     4801 cagtctagtt gacaactaga tagattctag attgaggcca aagtctctga gatccaggtt
     4861 agttgcaact aatactagtt agtatctagt ctcctataac tctgaagcta gaataactta
     4921 ctactattat cctcaccact gttcagctgc gcaaacggag tgattgcaag gtgttcagag
     4981 actagttatt gactagtcag tgactagcaa taactaacaa ggtattaacc taccatgtct
     5041 gccatcaccc tgcacttcct cgggctcagc agccttttcc tcctcatttt catgctcatt
     5101 ttccttgttt aagactgtga ctagtcgaag actagtccag aaccacaaag gagaaatgtc
     5161 ttaccacttt cttcattgct tgtctctttt gcattatcca tgtctgcaac tagttagagt
     5221 ctagttagtg actagtccga cgaggacttg cttgtctccg gattgttgga ggaactctcc
     5281 agggcctcaa gatccacaac agagccttct agaagactgg tcaataacta gttggtcttt
     5341 gtctgagtct gacttacgag gttgcatact cgctcccttt gcctcgtcaa tcgatgagaa
     5401 aaagcgccaa aactcgcaat atggctttga accacacggt gctgagacta gttagaatct
     5461 agtcccaaac tagcttggat agcttacctt tgccctttgc gttgcgacag gtcttgcagg
     5521 gtatggttcc tttctcacca gctgatttag ctgccttgct accctcacgg cggatctgca
     5581 taaagagtgg ctagaggtta taaattagca ctgatcctag gtacggggct gaatgtaact
     5641 tgcccttcct ttctcatcgc gcggcaagac aggcttgctc aaattcctac cagtcacagg
     5701 ggtatgcacg gcgtacggac cacttgaact agtcacagat tagttagcaa ctagtctgca
     5761 ttgaatggct gtacttacgg gccctcgcca ttgtcctgat catttccagc ttcaccctcg
     5821 ttgctgcaaa gtagttagtg actagtcaag gactagttga aatgggagaa gaaactcacg
     5881 aattctcgac acccttagta ttgtggtcct tggacttggt gctgctatat attagctaat
     5941 acactagtta gactcacgga aacttacgca gctcgcttgc gcttcttggt aggagtcggg
     6001 gttgggagaa cagtgccttc aaacaagcct tcataccatg ctacttgact agtcagggac
     6061 tagtcaccaa gtaatctaga taggacttgc ctttggcctc catcagttcc ttcatagtgg
     6121 gaggtccatt gtgcaatgta aactccatgc cgtgggagtt cttgtccttc aagtgcttga
     6181 ccaatatgtt tctgttggca gagggaacct gtcaactagt taataactag tcagaaacta
     6241 gtatagcagt agactcactg tacgcttgag gcatcccttc actcggcagt agacttcata
     6301 tggatggata tcaggcacgc cattgtcgtc ctgtggacta gtcagtaact aggcttaaag
     6361 ctagtcgggt cggcttacta tcttgaaatc cggcagcgta agctccccgt ccttaactgc
     6421 ctcgagatag tgacagtact ctggggactt tcggagatcg ttatcgcgaa tgctcggcat
     6481 actaatcgtt gactagtctt ggactagtcc cgagcaaaaa ggattggagg aggaggagga
     6541 aggtgagagt gagacaaaga gcgaaataag agcttcaaag gctatctcta agcagtatga
     6601 aggttaagta tctagttctt gactagattt aaaagagatt tcgactagtt atgtacctgg
     6661 agtttggata taggaatgtg ttgtggtaac gaaatgtaag ggggaggaaa gaaaaagtcg
     6721 gtcaagaggt aactctaagt cggccattcc tttttgggag gcgctaacca taaacggcat
     6781 ggtcgactta gagttagctc agggaattta gggagttatc tgcgaccact ggactctcac
     6841 cgtttccatt ggggtactaa catagccatc aaatgccggc ccgtgtttaa tctcctcgag
     6901 atggctgggc tcacatgcgt tctactcaaa aactcttata cctaggccct ctcccaatcg
     6961 tgtatgtttg gaccttcctc tctcacactt tggataacat caagcatact gcagattttg
     7021 acctagttaa caaggcacat catgctatca cttccaacac tcttgctgag cgcgttctgc
     7081 cttgcctttc tggttctacg aagtatccag tcccgcctgc aacgccgcag aaacgccaaa
     7141 aggtggggtt gtcaaccgcc agctagagct ccatcgggat tctttggcat tccgccgttc
     7201 ctcggtggtc gcagataact ccctaaattc cctgagctaa ctctaagtcg accatgccgt
     7261 ttatggttag cgcctcccaa aaaggaatgg ccgacttaga gttacctctt gaccgacttt
     7321 ttctttcctc ccccttacat ttcgttacca caacacattc ctatatccaa actccaggta
     7381 cataactagt cgaaatctct tttaaatcta gtcaagaact agatacttaa ccttcatact
     7441 gcttagagat agcctttgaa gctcttattt cgctctttgt ctcactctca ccttcctcct
     7501 cctcctccaa tcctttttgc tcgggactag tccaagacta gtcaacgatt agtatgccga
     7561 gcattcgcga taacgatctc cgaaagtccc cagagtactg tcactatctc gaggcagtta
     7621 aggacgggga gcttacgctg ccggatttca agatagtaag ccgacccgac tagctttaag
     7681 cctagttact gactagtcca caggacgaca atggcgtgcc tgatatccat ccatatgaag
     7741 tctactgccg agtgaaggga tgcctcaagc gtacagtgag tctactgcta tactagtttc
     7801 tgactagtta ttaactagtt gacaggttcc ctctgccaac agaaacatat tggtcaagca
     7861 cttgaaggac aagaactccc acggcatgga gtttacattg cacaatggac ctcccactat
     7921 gaaggaactg atggaggcca aaggcaagtc ctatctagat tacttggtga ctagtccctg
     7981 actagtcaag tagcatggta tgaaggcttg tttgaaggca ctgttctccc aaccccgact
     8041 cctaccaaga agcgcaagcg agctgcgtaa gtttctgtga gtctaactag tgtattagct
     8101 aatatatagc agcaccaagt ccaaggacca caatactaag ggtgtcgaga attcgtgagt
     8161 ttcttctccc atttcaacta gtccttgact agtcactaac tactttgcag caacgagggt
     8221 gaagctggaa atgatcagga caatggcgag ggcccgtaag tacagccatt caatgcagac
     8281 tagttgctaa ctaatctgtg actagttcaa gtggtccgta cgccgtgcat acccctgtga
     8341 ctggtaggaa tttgagcaag cctgtcttgc cgcgcgatga gaaaggaaag gcaagttaca
     8401 ttcagccccg tacctaggat cagtgctaat ttataacctc tagccactct ttatgcagat
     8461 ccgccgtgag ggtagcaagg cagctaaatc agctggtgag aaaggaacca taccctgcaa
     8521 gacctgtcgc aacgcaaagg gcaaaggtaa gctatccaag ctagtttggg actagattct
     8581 aactagtctc agcaccgtgt ggttcaaagc catattgcga gttttggcgc tttttctcat
     8641 cgattgacga ggcaaaggga gcgagtatgc aacctcgtaa gtcagactca gacaaagacc
     8701 aactagttat tgaccagtct tctagaaggc tctgttgtgg atcttgaggc cctggagagt
     8761 tcctccaaca atccggagac aagcaagtcc tcgtcggact agtcactaac tagactctaa
     8821 ctagttgcag acatggataa tgcaaaagag acaagcaatg aagaaagtgg taagacattt
     8881 ctcctttgtg gttctggact agtctttgac tagtcacagt cttaaacaag gaaaatgagc
     8941 atgaaaatga ggaggaaaag gctgctgagc ccgaggaagt gcagggtgat ggcagacatg
     9001 gtaggttaat accttgttag ttattgctag tcactgacta gtcaataact agtctctgaa
     9061 caccttgcaa tcactccgtt tgcgcagctg aacagtggtg aggataatag tagtaagtta
     9121 ttctagcttc agagttatag gagactagat actaactagt attagttgca actaacctgg
     9181 atctcagaga ctttggcctc aatctagaat ctatctagtt gtcaactaga ctgtggtatc
     9241 attgtctttt attttcctag tcctggaact agcttctaac tagtctccct aatatgtggc
     9301 tgtcttgttt ttttttttgt ttccctaccc ggatatctag tccccttcta ggttctgtta
     9361 acctctcggg ctctgattta gtttaacgca aacctgagat tagtttctaa ctagtctcta
     9421 ggttttctat ccacctttaa ttgtaataat aaatacaagc aacgtttata cgtcaaaagc
     9481 atttataaac ttttacccta aagtagcttg cttgtgtgtt tagtttataa ttagtctctt
     9541 attaatttga tgtaggtaag cccgccacaa atatatattt ttaacaagat accgtggaaa
     9601 aacttcgtgc tatcacaaaa cagtatacaa aaaataagct taacaatcta ttctccgctt
     9661 ggtgatgcta aagggctttc aatagacctt gtaagtgaag gagatggagc cgtcaatccg
     9721 ctaccctgcc tctggtcagt tggtctcagc aatgtaccct gtgagtcttg atagactagt
     9781 tggtgactag tctctgtaga tggaacaaat ggtgcttgag agggcacgta tggcagagct
     9841 ccagtcatct gcggaacata tactgggccc ggtacgcgtt aagcttggca ctggccgtca
     9901 gctgcttcgt cgattaaacc ctcagcgcgg ccgcatttaa atccccactt cagaagttcc
     9961 tatactttct agagaatagg aacttcacta tagagtcgaa taagggcgac accccataat
    10021 tagcccgggc gaaaggccca gtctttcgac tgagcctttc gttttatttg atgcctggca
    10081 gttccctact ctcgcatggg gagtccccac actaccatcg gcgctacggc gtttcacttc
    10141 tgagttcggc atggggtcag gtgggaccac cgcgctactg ccgccaggca aacaaggggt
    10201 gttatgagcc atattcaggt ataaatgggc tcgcgataat gttcagaatt ggttaattgg
    10261 ttgtaacact gacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
    10321 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagaat atgagtattc
    10381 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
    10441 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
    10501 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
    10561 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
    10621 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
    10681 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgtagcgctg
    10741 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
    10801 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
    10861 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcga
    10921 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
    10981 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
    11041 cggctggctg gtttattgct gataaatccg gagccggtga gcgtggttct cgcggtatca
    11101 tcgcagcgct ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
    11161 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
    11221 agcattggta agcagagcat tacgctgact tgacgggacg gcgcaagctc atgaccaaaa
    11281 tcccttaacg tgagttacgc gcgcgtcgtt ccactgagcg tcagaccccg tagaaaagat
    11341 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
    11401 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
    11461 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
    11521 agcccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
    11581 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
    11641 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
    11701 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
    11761 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
    11821 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
    11881 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
    11941 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
    12001 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
    12061 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
    12121 aggcgagagt agggaactgc caggcatcaa actaagcaga aggcccctga cggatggcct
    12181 ttttgcgttt ctacaaactc tttctgtgtt gtaaaacgac ggccagtctt aagctcgggc
    12241 cccctgggcg gttctgataa cgagtaatcg ttaatccgca aataacgtaa aaacccgctt
    12301 cggcgggttt ttttatgggg ggagtttagg gaaagagcat ttgtcagaat atttaagggc
    12361 gcctgtcact ttgcttgata tatgagaatt atttaacctt ataaatgaga aaaaagcaac
    12421 gcactttaaa taagatacgt tgctttttcg attgatgaac acctataatt aaactattca
    12481 tctattattt atgatttttt gtatatacaa tatttctagt ttgttaaaga gaattaagaa
    12541 aataaatctc gaaaataata aagggaaaat cagtttttga tatcaaaatt atacatgtca
    12601 acgataatac aaaatataat acaaactata agatgttatc agtatttatt atgcatttag
    12661 aataaatttt gtgtcgccct tattcgactc actatagaag ttcctattct ctagaaagta
    12721 taggaacttc acttcatttt atttaaattt gcggccgcgc tgaggtctta atgcggtagt
    12781 ggggatgcct caattgtgct agctgcggcc gccttgctag atgactggta ggaatctaac
    12841 aaagggaaaa ccttgacgat tgggggcgga aaacatggca ttacaggggc caatacggac
    12901 gagctcatcg tttaatgaca ggcacaatgt atgcagcctt cctccacaac actcgtactg
    12961 gcactacact tttgctccaa gaggaaatca tgacttgccg catactctgg ccatataagc
    13021 ttcccagcct tctttctggt accgctgtgc agcttcaacc gggtcgggag cagcgtagat
    13081 gcctcgaccg gcgatgataa agtcggcacc gcgtccaata gccgatgcag gagtctggta
    13141 ttgctgtcca agcttatctc ctttggaaga gaggttcaca cccgtcgtga agaccacgaa
    13201 atcttcatcc tccgaggctg aagacacatc cgactgcact tccgtcaggg cccgcgtcga
    13261 cacgaaaccc ataacgaagt tcttgtattt gcgagcgtag tcaaccgatg ccttggtata
    13321 ctcgcccgta gccagcgatc ctttgggggt catctctgcc aggaccaaca gtcctctctc
    13381 aggaccatag gggaagtctt gcgcagatgc ggtctgggcc agagcctcga cgatgccctc
    13441 gccagggaga acgctgcagt tgataatgtg ggcccattcg gagatcctca gagcaccgcc
    13501 gtggtattgc ttctggacgg tattgccgat gtcgatgaat ttgcggtcct cgaagatcaa
    13561 aaagttgtac ttttgagcca gcacattcag gccattgata gtgtcgacgc tgaaatcggt
    13621 gaggatgtcg atgtgtgtct tgatgacggc gatgtaggga ccgagacctg tatcatcaaa
    13681 agttgattag tccattgcta gacggcatat gtattggatc caacagcttc cgtacggtca
    13741 gcgaggtcca ggagttctcg ggttgtcgtc acatcagcag agacggtaac gtttgtcttc
    13801 tttgcttcgg caatctcaaa aagtctcttt gccagaggat tggggtgctt gctggctcga
    13861 gcaccgtaag tcaattgcga cttggacgac atcgtgggaa tggagggtta tatgcggggt
    13921 atgacggtga ttgatgagtt gaagtagccg agcaatgagg tatattatcc aattgagggt
    13981 gacccaacca aatcaattgc ttgagaatca aatctcaaat attctaaaat agaagccgcc
    14041 ggggactaaa tttaaagtcg tactctatct cgatcttttc cgcgtgtaaa aattgcgtgc
    14101 caatctggat ggagacaggc cacatcggtg ctgtattcct ccgcctccga ctgtggggtc
    14161 aaccacaatt aaactactct gtagtcaggt acagctagaa tggggtagac aggcagaact
    14221 gtagaagata aaacattggt caatcactgg taactccacg tctagaggat ccccggccgg
    14281 gtaccgagct cgaattcgta atcatgtcat attacctagt gggcgcttac acagtacacg
    14341 aggaattaca cttgtattgg gatgaatttt gtatgcacgc gctgcgggcg caattttttg
    14401 aaattttcca tttttaatgt cttgaatcgc gcattggatc ctgccctctt gctcaatcaa
    14461 atctcgcttt gttttacgcg cttttggtgg catgatggtt gttgaaagtt gaggttgata
    14521 aacgcgttgg ggtggacgag aaaactacct tccgcccggg acgcacctac cgcccgggat
    14581 ttacgttatg actttcgcgc agaactctgt ccaggtcatg taatatcacg tgataagcaa
    14641 ggtacacgca aaacgaggta catttacgaa tagtttcaac attggcttta cgggaagagc
    14701 tgagattaat aatgaacagc ggatttgaag ttcaggaatc ataatccatt gtagaatcaa
    14761 ctacataaca agtctaattc catttctttc attcgtaaag gcttttttaa ggaagtcata
    14821 acggcataaa tcgaatgtcc gctcagacct tgcgcttctt cttgggaggg tcgcccccca
    14881 gttgactaag gtcgatacgg gtttcataga gcccagtgat ggattgatga atcagggtcg
    14941 catcaagcac ctcctttgtg gacgtgtatc gcttacggtc gattgtcgta tcaaaatact
    15001 tgaacgctgc tggtgctccc aagttagtga gggtgaacag atgaatgata ttttcagcct
    15061 gctctcggat cggcttgtca cggtgcttgt tgtaggccga caacaccttg tcgagattag
    15121 catcggcgag gatgacgcgc ttactgaact ctgaaatctg ttcaatgatc tcatccagat
    15181 aatgcttgtg ttgctcaaca aaaagttgct tctgttcgtt gtcctcaggg ctacccttca
    15241 gcttctcgta gtgggacgcg agatacagga agttcacgta ctttgaggga agtgccaatt
    15301 cattcccctt ctgcaactct cctgccgatg cgagcattcg ctttcgaccg ttttcaagct
    15361 cgaacaaaga gtacttaggc agcttaatga taagatcctt cttgacttcc ttatagccct
    15421 tggcctcgag aaagtcgatg ggattctttt cgaagctgga gcgctccata atggtgatcc
    15481 cgagcagttc cttgaccgac ttcagcttct tagactttcc cttctcaacc ttcgcgacaa
    15541 cgagaaccga gtatgccact gtaggagaat cgaaccctcc atacttcttg gggtcccaat
    15601 ccttctttcg agcgatcagc ttgtcagagt tacgctttgg aagaatcgat tccttagaga
    15661 aaccgcctgt ctgcacctcg gttttcttga cgatattgac ttgcggcatg ctcagaacct
    15721 tccgcacggt agcgaagtcg cgccccttat cccaaacgat ttcaccagtc tcgccgttgg
    15781 tctcaatcaa aggtcgctta cggatttctc cattggcgag tgtgatctcc gtcttaaaga
    15841 aattcatgat gttggagtaa aagaaatact tagccgtggc cttgccaatt tcctgctcac
    15901 tcttggcgat catcttgcgg acgtcgtaaa ccttataatc cccgtagacg aattcggact
    15961 ccaacttggg atacttcttg atgagcgcag ttcccacgac tgcgttgagg tacgcatcat
    16021 gtgcatggtg ataattgttg atctcccgaa ccttataaaa ttggaagtcc ttgcggaaat
    16081 ccgacaccaa cttagacttg agggtaatga ccttaacttc acggatgagc ttatcattct
    16141 cgtcgtactt tgtgttcatc cgggagtcca gaatctgagc aacgtgcttt gtgatctgtc
    16201 gcgtctccac gagttgacgc ttgatgaagc cagccttatc gagttcactc agccctcccc
    16261 gctcggcctt ggtcaagttg tcaaacttgc gctgtgtgat gagcttggca ttaagcaatt
    16321 ggcgccagta gttcttcatc ttcttaacca cttcctcgct tggaacattg tcggacttgc
    16381 cccggttctt atctgagcga gtcagcacct tgttatcaat gctatcgtcc ttaaggaagg
    16441 attgcgggac gatatggtca acatcgtagt ccgacaaccg gttaatgtcg agctcctgat
    16501 cgacgtacat gtcgcgtcca ttttgcaaat agtagagata cagcttctca ttttggagct
    16561 gtgtgttttc aacagggtgc tccttcagga tctgagaacc aagttcctta atgccttcct
    16621 cgatccgctt catccgctcg cggctgttct tctgaccctt ttgagtggtc tgattttctc
    16681 gggccatctc aatcacgatg ttttctggct tatgacggcc catcaccttg accagctcgt
    16741 cgacaacctt gacggtctga agaatcccct tcttgatcgc cggggatcca gccaggttgg
    16801 cgatatgctc gtgcagggaa tccccttgtc ctgagacttg tgccttctgg atgtcctcct
    16861 t
//
