LOCUS       sequence-503025-       16690 bp    DNA     circular SYN 16-MAR-2026
DEFINITION  CRISPR-Cas9 vector containing a 20 nt protospacer sequence to target
            mCitrine. pyrG marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16690)
  TITLE     Mortensen Lab plasmids
REFERENCE   2  (bases 1 to 16690)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 16, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pDIV1049
FEATURES             Location/Qualifiers
     source          1..16690
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(566..586)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(590..4693)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(590..4693)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     misc_RNA        complement(5895..5970)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     rep_origin      6803..12050
                     /label=AMA1
                     /note="replication origin from Aspergillus nidulans 
                     (Aleksenko and Clutterbuck, 1996)"
     protein_bind    complement(12363..12396)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(12603..12622)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(12628..12647)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        12689..12780
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12781..12849,12850..13641)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12999..13018)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      13763..14351
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     14252..14271
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     14505..14522
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     14621..14637
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    15107..15140
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 gtaccgagct cgaattcgta atcatgtcat attacctagt gggcgcttac acagtacacg
       61 aggaattaca cttgtattgg gatgaatttt gtatgcacgc gctgcgggcg caattttttg
      121 aaattttcca tttttaatgt cttgaatcgc gcattggatc ctgccctctt gctcaatcaa
      181 atctcgcttt gttttacgcg cttttggtgg catgatggtt gttgaaagtt gaggttgata
      241 aacgcgttgg ggtggacgag aaaactacct tccgcccggg acgcacctac cgcccgggat
      301 ttacgttatg actttcgcgc agaactctgt ccaggtcatg taatatcacg tgataagcaa
      361 ggtacacgca aaacgaggta catttacgaa tagtttcaac attggcttta cgggaagagc
      421 tgagattaat aatgaacagc ggatttgaag ttcaggaatc ataatccatt gtagaatcaa
      481 ctacataaca agtctaattc catttctttc attcgtaaag gcttttttaa ggaagtcata
      541 acggcataaa tcgaatgtcc gctcagacct tgcgcttctt cttgggaggg tcgcccccca
      601 gttgactaag gtcgatacgg gtttcataga gcccagtgat ggattgatga atcagggtcg
      661 catcaagcac ctcctttgtg gacgtgtatc gcttacggtc gattgtcgta tcaaaatact
      721 tgaacgctgc tggtgctccc aagttagtga gggtgaacag atgaatgata ttttcagcct
      781 gctctcggat cggcttgtca cggtgcttgt tgtaggccga caacaccttg tcgagattag
      841 catcggcgag gatgacgcgc ttactgaact ctgaaatctg ttcaatgatc tcatccagat
      901 aatgcttgtg ttgctcaaca aaaagttgct tctgttcgtt gtcctcaggg ctacccttca
      961 gcttctcgta gtgggacgcg agatacagga agttcacgta ctttgaggga agtgccaatt
     1021 cattcccctt ctgcaactct cctgccgatg cgagcattcg ctttcgaccg ttttcaagct
     1081 cgaacaaaga gtacttaggc agcttaatga taagatcctt cttgacttcc ttatagccct
     1141 tggcctcgag aaagtcgatg ggattctttt cgaagctgga gcgctccata atggtgatcc
     1201 cgagcagttc cttgaccgac ttcagcttct tagactttcc cttctcaacc ttcgcgacaa
     1261 cgagaaccga gtatgccact gtaggagaat cgaaccctcc atacttcttg gggtcccaat
     1321 ccttctttcg agcgatcagc ttgtcagagt tacgctttgg aagaatcgat tccttagaga
     1381 aaccgcctgt ctgcacctcg gttttcttga cgatattgac ttgcggcatg ctcagaacct
     1441 tccgcacggt agcgaagtcg cgccccttat cccaaacgat ttcaccagtc tcgccgttgg
     1501 tctcaatcaa aggtcgctta cggatttctc cattggcgag tgtgatctcc gtcttaaaga
     1561 aattcatgat gttggagtaa aagaaatact tagccgtggc cttgccaatt tcctgctcac
     1621 tcttggcgat catcttgcgg acgtcgtaaa ccttataatc cccgtagacg aattcggact
     1681 ccaacttggg atacttcttg atgagcgcag ttcccacgac tgcgttgagg tacgcatcat
     1741 gtgcatggtg ataattgttg atctcccgaa ccttataaaa ttggaagtcc ttgcggaaat
     1801 ccgacaccaa cttagacttg agggtaatga ccttaacttc acggatgagc ttatcattct
     1861 cgtcgtactt tgtgttcatc cgggagtcca gaatctgagc aacgtgcttt gtgatctgtc
     1921 gcgtctccac gagttgacgc ttgatgaagc cagccttatc gagttcactc agccctcccc
     1981 gctcggcctt ggtcaagttg tcaaacttgc gctgtgtgat gagcttggca ttaagcaatt
     2041 ggcgccagta gttcttcatc ttcttaacca cttcctcgct tggaacattg tcggacttgc
     2101 cccggttctt atctgagcga gtcagcacct tgttatcaat gctatcgtcc ttaaggaagg
     2161 attgcgggac gatatggtca acatcgtagt ccgacaaccg gttaatgtcg agctcctgat
     2221 cgacgtacat gtcgcgtcca ttttgcaaat agtagagata cagcttctca ttttggagct
     2281 gtgtgttttc aacagggtgc tccttcagga tctgagaacc aagttcctta atgccttcct
     2341 cgatccgctt catccgctcg cggctgttct tctgaccctt ttgagtggtc tgattttctc
     2401 gggccatctc aatcacgatg ttttctggct tatgacggcc catcaccttg accagctcgt
     2461 cgacaacctt gacggtctga agaatcccct tcttgatcgc cggggatcca gccaggttgg
     2521 cgatatgctc gtgcagggaa tccccttgtc ctgagacttg tgccttctgg atgtcctcct
     2581 tgaacgtcag ggaatcgtcg tgaataagct gcataaaatt gcggttggcg aagccgtccg
     2641 acttaaggaa atccaagata gtcttaccag attgcttatc tcgaatgccg ttgatcagct
     2701 tccggctaag gcgaccccat ccggtgtatc gacgccgctt cagctgcttc atcaccttat
     2761 cgtcgaacaa atgggcatat gtcttgagcc gttcctcgat catttcgcgg tcctcaaaaa
     2821 gtgtcaacgt gaggacaata tcttccaaga tgtcctcatt ttcctcgtta tcgaggaaat
     2881 ccttgtcctt aatgatcttg agcaggtcgt ggtaggtacc gagggaagcg ttaaagcgat
     2941 cttccacgcc gctgatctcg acggagtcga aacattcaat cttcttgaaa taatcctcct
     3001 tcagctgctt aacagtcact ttacgattgg tcttaaaaag caaatcgacg atagccttct
     3061 tttgttcccc tgagaggaag gctggcttcc gcattccctc cgtcacgtac ttgaccttag
     3121 tcagttcgtt atacacggtg aaatactcgt agagcagcga atgctttggc aggacctttt
     3181 cattcggaag gttcttatca aaattggtca tccgctcaat gaagctctgc gcggatgcac
     3241 ccttgtccac gacttcctcg aagttccacg gagtgatggt ttcctcgctc tttcgggtca
     3301 tccaagcaaa tcgggaattc ccacgggcca aaggtcccac atagtagggg attcggaatg
     3361 taagaatctt ttcgatcttc tcacggttgt ccttcagaaa tgggtagaaa tcttcctggc
     3421 gtcgaaggat ggcgtggagc tcgcccagat gaatctggtg cgggatggag ccgttgtcga
     3481 acgttcgttg cttacgaagc aaatcttcgc gattcagctt gacgagcagt tcctcagtgc
     3541 cgtccatctt ctccaaaatc ggcttgataa acttgtagaa ttcctcctgg ctcgcaccgc
     3601 catcaatata ccctgcgtat ccgttcttgg attggtcaaa gaagatttcc ttatacttct
     3661 caggcaattg ctgacgcacg agggccttaa gcaaggtgag gtcctgatgg tgctcatcat
     3721 accgcttgat catactagct gacagggggg ccttagtaat ctcggtgttg acccgcaaaa
     3781 tgtcactgag caggattgca tctgagagat tcttagcggc caggaaaaga tcagcgtact
     3841 ggtcaccgat ttgggcaagc aagttgtcga gatcgtcatc atacgtatcc ttagacaact
     3901 ggagcttcgc atcctctgcc aggtcaaaat tactcttgaa gttaggagtc agaccaagtg
     3961 acaacgcaat caaattaccg aagaggccgt tcttcttttc gccggggagt tgtgcgataa
     4021 ggttctccaa acgccgcgac ttagacaggc gcgccgaaag gatagccttg gcgtccacgc
     4081 cagatgcatt aatagggttt tcctcaaaca gctgattgta ggtttggacc aactggatga
     4141 agagcttatc aacgtcagaa ttatcggggt tcaggtcacc ctcgataaga aaatggccgc
     4201 ggaacttaat catgtgcgcg agtgccaggt agataagtcg caaatcagcc ttgtccgtgc
     4261 tatcgaccag cttcttacga agatgataga tagttgggta cttttcgtga taggcaacct
     4321 catccacaat gttaccaaag atcggatggc gctcgtgctt cttatcctcc tcgaccaaga
     4381 aactttcttc gagtcgatga aagaatgagt catcaacctt agccatttcg ttgctgaaaa
     4441 tctcctggag gtagcagatg cgattcttgc gtcgtgtata acgccggcga gccgttcgct
     4501 tcagtcgagt agcttcggcg gtctccccac tatcgaagag cagggctcca atgagattct
     4561 tcttgattga gtgacggtcg gtgtttccca ggaccttaaa cttcttggat ggcaccttgt
     4621 attcgtccgt aatcacagcc caaccaaccg agttcgttcc aatgtccagc ccgatgctat
     4681 acttcttgtc catggtgaag gttgtgttat gttttgtgga atataaaagg gagaagtgct
     4741 cagatactac ggctagtaat gcgtagaggt gctgagcaga gaagttagca ggagatggat
     4801 gaaagaagga tggaggagag aagcgaggga agggaagata tacgtactga agcggacaag
     4861 gagaggaagg gaaagaaaat tttgtgggag ggggagggga aatcctttta tggaggggca
     4921 agcgaggacg acgactaagc gggcgggctc aaacccttgg ctggtagata tctgtcattg
     4981 gatatggagg ggtaacccgc cccactcccg atgcaaattc ttctcctaga ctcccacctt
     5041 gggcgtcgag cctttacaac accttccgga acggcagccc agcgcgactc tttccttggt
     5101 tctgctccac cccatctcgt tttcatggct taggggcaga aatttttttt tgtttagctt
     5161 ttctttactt gctatcgcct ctacggtgga ttctcggatc gaaaaagctg ggcatcaacg
     5221 cgttgatggt cgcatggcgg cttttatcac tctgtacttt gcagcggaca accccgcggc
     5281 tgtacgagtt tgccgccttt taatttgtct gcgaccagcc tgcgatggag cgagcgagtg
     5341 gcgttgcttg atccacagcg tcaagatcaa catacgtagc aagatagacc gcaagaaaat
     5401 gatgctcgga atccatattg actcacgaaa atttctttct ttttgccccc ctcatctgtt
     5461 gcctgggagg tgagtcaaat tgcccctcac taaacgctcc tttgcttttc ccgaacttgg
     5521 cccgttcgag agcatgatca gcacaaaggc ttaacttggg cggtgattct gctgtctcgg
     5581 ctgaggtctt aataccctga gaagatagat gtgaatgtgt gcatagacat gcaggtcaat
     5641 gagcaattta atgtgattct atgaataaag ttgacggcaa tctcaatcta caaatagtct
     5701 acttggaagt aaaatgaact agtcatgtaa aaattatatt cttcagaatc taaaacaaga
     5761 gagaatttga gcaaactctg atcgcctgat tacaccaaca tcaagtgtaa aaattgacat
     5821 gcatcatccg tgaatcgaac acgggcctca tcgatggcaa cgatgaattc taccactaga
     5881 ccaatgatgc aaaagcaccg actcggtgcc actttttcaa gttgataacg gactagcctt
     5941 attttaactt gctatttcta gctctaaaac gcagaacacc cccatcggcg tgcatcatcc
     6001 gtgaatcgaa cacgggcctc atcgatggca acgatgaatt ctaccactag accaatgatg
     6061 cgctgttagc aaaatgttct gacttgcagt tgagaggttt tcccaaaggt agggaggggg
     6121 atgacatacg tagagtcgct tgatttccaa aatgcatggg atttcggggc cttttatatt
     6181 tgcgggcagg cgctagcgag tgcgctaacc tcaagcgaaa attttcatgt ttgctcggtt
     6241 tgttctgtgg ggctacctgt ggtaccaatc agagttagtt gaagattttc cgctccactg
     6301 gccagactgt ccccgagctc accggtgaat gggctccagc gtaaaatttc aacatcaggg
     6361 atggtctccc aaatatttgc ttctcagtgg ctgttgagcg acttcatgag gatgcgatga
     6421 caaacagtaa ctcactgata tcctgattgg atttgaagcc aagcgttagg agaacctcct
     6481 acaaagtact tgctataggt ggtcctgtca accgagcatc tatgtgatca ttaaaccctc
     6541 agcggaaaca gctatgacca tgagatcttc ccgggcccag tatatgttcc gcagatgact
     6601 ggagctctgc catacgtgcc ctctcaagca ccatttgttc catctacaga gactagtcac
     6661 caactagtct atcaagactc acagggtaca ttgctgagac caactgacca gaggcagggt
     6721 agcggattga cggctccatc tccttcactt acaaggtcta ttgaaagccc tttagcatca
     6781 ccaagcggag aatagattgt taagcttatt ttttgtatac tgttttgtga tagcacgaag
     6841 tttttccacg gtatcttgtt aaaaatatat atttgtggcg ggcttaccta catcaaatta
     6901 ataagagact aattataaac taaacacaca agcaagctac tttagggtaa aagtttataa
     6961 atgcttttga cgtataaacg ttgcttgtat ttattattac aattaaaggt ggatagaaaa
     7021 cctagagact agttagaaac taatctcagg tttgcgttaa actaaatcag agcccgagag
     7081 gttaacagaa cctagaaggg gactagatat ccgggtaggg aaacaaaaaa aaaaaacaag
     7141 acagccacat attagggaga ctagttagaa gctagttcca ggactaggaa aataaaagac
     7201 aatgatacca cagtctagtt gacaactaga tagattctag attgaggcca aagtctctga
     7261 gatccaggtt agttgcaact aatactagtt agtatctagt ctcctataac tctgaagcta
     7321 gaataactta ctactattat cctcaccact gttcagctgc gcaaacggag tgattgcaag
     7381 gtgttcagag actagttatt gactagtcag tgactagcaa taactaacaa ggtattaacc
     7441 taccatgtct gccatcaccc tgcacttcct cgggctcagc agccttttcc tcctcatttt
     7501 catgctcatt ttccttgttt aagactgtga ctagtcgaag actagtccag aaccacaaag
     7561 gagaaatgtc ttaccacttt cttcattgct tgtctctttt gcattatcca tgtctgcaac
     7621 tagttagagt ctagttagtg actagtccga cgaggacttg cttgtctccg gattgttgga
     7681 ggaactctcc agggcctcaa gatccacaac agagccttct agaagactgg tcaataacta
     7741 gttggtcttt gtctgagtct gacttacgag gttgcatact cgctcccttt gcctcgtcaa
     7801 tcgatgagaa aaagcgccaa aactcgcaat atggctttga accacacggt gctgagacta
     7861 gttagaatct agtcccaaac tagcttggat agcttacctt tgccctttgc gttgcgacag
     7921 gtcttgcagg gtatggttcc tttctcacca gctgatttag ctgccttgct accctcacgg
     7981 cggatctgca taaagagtgg ctagaggtta taaattagca ctgatcctag gtacggggct
     8041 gaatgtaact tgcccttcct ttctcatcgc gcggcaagac aggcttgctc aaattcctac
     8101 cagtcacagg ggtatgcacg gcgtacggac cacttgaact agtcacagat tagttagcaa
     8161 ctagtctgca ttgaatggct gtacttacgg gccctcgcca ttgtcctgat catttccagc
     8221 ttcaccctcg ttgctgcaaa gtagttagtg actagtcaag gactagttga aatgggagaa
     8281 gaaactcacg aattctcgac acccttagta ttgtggtcct tggacttggt gctgctatat
     8341 attagctaat acactagtta gactcacgga aacttacgca gctcgcttgc gcttcttggt
     8401 aggagtcggg gttgggagaa cagtgccttc aaacaagcct tcataccatg ctacttgact
     8461 agtcagggac tagtcaccaa gtaatctaga taggacttgc ctttggcctc catcagttcc
     8521 ttcatagtgg gaggtccatt gtgcaatgta aactccatgc cgtgggagtt cttgtccttc
     8581 aagtgcttga ccaatatgtt tctgttggca gagggaacct gtcaactagt taataactag
     8641 tcagaaacta gtatagcagt agactcactg tacgcttgag gcatcccttc actcggcagt
     8701 agacttcata tggatggata tcaggcacgc cattgtcgtc ctgtggacta gtcagtaact
     8761 aggcttaaag ctagtcgggt cggcttacta tcttgaaatc cggcagcgta agctccccgt
     8821 ccttaactgc ctcgagatag tgacagtact ctggggactt tcggagatcg ttatcgcgaa
     8881 tgctcggcat actaatcgtt gactagtctt ggactagtcc cgagcaaaaa ggattggagg
     8941 aggaggagga aggtgagagt gagacaaaga gcgaaataag agcttcaaag gctatctcta
     9001 agcagtatga aggttaagta tctagttctt gactagattt aaaagagatt tcgactagtt
     9061 atgtacctgg agtttggata taggaatgtg ttgtggtaac gaaatgtaag ggggaggaaa
     9121 gaaaaagtcg gtcaagaggt aactctaagt cggccattcc tttttgggag gcgctaacca
     9181 taaacggcat ggtcgactta gagttagctc agggaattta gggagttatc tgcgaccact
     9241 ggactctcac cgtttccatt ggggtactaa catagccatc aaatgccggc ccgtgtttaa
     9301 tctcctcgag atggctgggc tcacatgcgt tctactcaaa aactcttata cctaggccct
     9361 ctcccaatcg tgtatgtttg gaccttcctc tctcacactt tggataacat caagcatact
     9421 gcagattttg acctagttaa caaggcacat catgctatca cttccaacac tcttgctgag
     9481 cgcgttctgc cttgcctttc tggttctacg aagtatccag tcccgcctgc aacgccgcag
     9541 aaacgccaaa aggtggggtt gtcaaccgcc agctagagct ccatcgggat tctttggcat
     9601 tccgccgttc ctcggtggtc gcagataact ccctaaattc cctgagctaa ctctaagtcg
     9661 accatgccgt ttatggttag cgcctcccaa aaaggaatgg ccgacttaga gttacctctt
     9721 gaccgacttt ttctttcctc ccccttacat ttcgttacca caacacattc ctatatccaa
     9781 actccaggta cataactagt cgaaatctct tttaaatcta gtcaagaact agatacttaa
     9841 ccttcatact gcttagagat agcctttgaa gctcttattt cgctctttgt ctcactctca
     9901 ccttcctcct cctcctccaa tcctttttgc tcgggactag tccaagacta gtcaacgatt
     9961 agtatgccga gcattcgcga taacgatctc cgaaagtccc cagagtactg tcactatctc
    10021 gaggcagtta aggacgggga gcttacgctg ccggatttca agatagtaag ccgacccgac
    10081 tagctttaag cctagttact gactagtcca caggacgaca atggcgtgcc tgatatccat
    10141 ccatatgaag tctactgccg agtgaaggga tgcctcaagc gtacagtgag tctactgcta
    10201 tactagtttc tgactagtta ttaactagtt gacaggttcc ctctgccaac agaaacatat
    10261 tggtcaagca cttgaaggac aagaactccc acggcatgga gtttacattg cacaatggac
    10321 ctcccactat gaaggaactg atggaggcca aaggcaagtc ctatctagat tacttggtga
    10381 ctagtccctg actagtcaag tagcatggta tgaaggcttg tttgaaggca ctgttctccc
    10441 aaccccgact cctaccaaga agcgcaagcg agctgcgtaa gtttctgtga gtctaactag
    10501 tgtattagct aatatatagc agcaccaagt ccaaggacca caatactaag ggtgtcgaga
    10561 attcgtgagt ttcttctccc atttcaacta gtccttgact agtcactaac tactttgcag
    10621 caacgagggt gaagctggaa atgatcagga caatggcgag ggcccgtaag tacagccatt
    10681 caatgcagac tagttgctaa ctaatctgtg actagttcaa gtggtccgta cgccgtgcat
    10741 acccctgtga ctggtaggaa tttgagcaag cctgtcttgc cgcgcgatga gaaaggaaag
    10801 gcaagttaca ttcagccccg tacctaggat cagtgctaat ttataacctc tagccactct
    10861 ttatgcagat ccgccgtgag ggtagcaagg cagctaaatc agctggtgag aaaggaacca
    10921 taccctgcaa gacctgtcgc aacgcaaagg gcaaaggtaa gctatccaag ctagtttggg
    10981 actagattct aactagtctc agcaccgtgt ggttcaaagc catattgcga gttttggcgc
    11041 tttttctcat cgattgacga ggcaaaggga gcgagtatgc aacctcgtaa gtcagactca
    11101 gacaaagacc aactagttat tgaccagtct tctagaaggc tctgttgtgg atcttgaggc
    11161 cctggagagt tcctccaaca atccggagac aagcaagtcc tcgtcggact agtcactaac
    11221 tagactctaa ctagttgcag acatggataa tgcaaaagag acaagcaatg aagaaagtgg
    11281 taagacattt ctcctttgtg gttctggact agtctttgac tagtcacagt cttaaacaag
    11341 gaaaatgagc atgaaaatga ggaggaaaag gctgctgagc ccgaggaagt gcagggtgat
    11401 ggcagacatg gtaggttaat accttgttag ttattgctag tcactgacta gtcaataact
    11461 agtctctgaa caccttgcaa tcactccgtt tgcgcagctg aacagtggtg aggataatag
    11521 tagtaagtta ttctagcttc agagttatag gagactagat actaactagt attagttgca
    11581 actaacctgg atctcagaga ctttggcctc aatctagaat ctatctagtt gtcaactaga
    11641 ctgtggtatc attgtctttt attttcctag tcctggaact agcttctaac tagtctccct
    11701 aatatgtggc tgtcttgttt ttttttttgt ttccctaccc ggatatctag tccccttcta
    11761 ggttctgtta acctctcggg ctctgattta gtttaacgca aacctgagat tagtttctaa
    11821 ctagtctcta ggttttctat ccacctttaa ttgtaataat aaatacaagc aacgtttata
    11881 cgtcaaaagc atttataaac ttttacccta aagtagcttg cttgtgtgtt tagtttataa
    11941 ttagtctctt attaatttga tgtaggtaag cccgccacaa atatatattt ttaacaagat
    12001 accgtggaaa aacttcgtgc tatcacaaaa cagtatacaa aaaataagct taacaatcta
    12061 ttctccgctt ggtgatgcta aagggctttc aatagacctt gtaagtgaag gagatggagc
    12121 cgtcaatccg ctaccctgcc tctggtcagt tggtctcagc aatgtaccct gtgagtcttg
    12181 atagactagt tggtgactag tctctgtaga tggaacaaat ggtgcttgag agggcacgta
    12241 tggcagagct ccagtcatct gcggaacata tactgggccc ggtacgcgtt aagcttggca
    12301 ctggccgtca gctgcttcgt cgattaaacc ctcagcgcgg ccgcatttaa atccccactt
    12361 cagaagttcc tatactttct agagaatagg aacttcacta tagagtcgaa taagggcgac
    12421 accccataat tagcccgggc gaaaggccca gtctttcgac tgagcctttc gttttatttg
    12481 atgcctggca gttccctact ctcgcatggg gagtccccac actaccatcg gcgctacggc
    12541 gtttcacttc tgagttcggc atggggtcag gtgggaccac cgcgctactg ccgccaggca
    12601 aacaaggggt gttatgagcc atattcaggt ataaatgggc tcgcgataat gttcagaatt
    12661 ggttaattgg ttgtaacact gacccctatt tgtttatttt tctaaataca ttcaaatatg
    12721 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagaat
    12781 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
    12841 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
    12901 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
    12961 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
    13021 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
    13081 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
    13141 tgtagcgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
    13201 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
    13261 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
    13321 cctgtagcga tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
    13381 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
    13441 tcggcccttc cggctggctg gtttattgct gataaatccg gagccggtga gcgtggttct
    13501 cgcggtatca tcgcagcgct ggggccagat ggtaagccct cccgtatcgt agttatctac
    13561 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
    13621 tcactgatta agcattggta agcagagcat tacgctgact tgacgggacg gcgcaagctc
    13681 atgaccaaaa tcccttaacg tgagttacgc gcgcgtcgtt ccactgagcg tcagaccccg
    13741 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
    13801 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
    13861 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
    13921 agccgtagtt agcccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
    13981 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
    14041 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
    14101 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
    14161 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
    14221 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
    14281 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
    14341 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
    14401 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
    14461 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
    14521 aggaagcgga aggcgagagt agggaactgc caggcatcaa actaagcaga aggcccctga
    14581 cggatggcct ttttgcgttt ctacaaactc tttctgtgtt gtaaaacgac ggccagtctt
    14641 aagctcgggc cccctgggcg gttctgataa cgagtaatcg ttaatccgca aataacgtaa
    14701 aaacccgctt cggcgggttt ttttatgggg ggagtttagg gaaagagcat ttgtcagaat
    14761 atttaagggc gcctgtcact ttgcttgata tatgagaatt atttaacctt ataaatgaga
    14821 aaaaagcaac gcactttaaa taagatacgt tgctttttcg attgatgaac acctataatt
    14881 aaactattca tctattattt atgatttttt gtatatacaa tatttctagt ttgttaaaga
    14941 gaattaagaa aataaatctc gaaaataata aagggaaaat cagtttttga tatcaaaatt
    15001 atacatgtca acgataatac aaaatataat acaaactata agatgttatc agtatttatt
    15061 atgcatttag aataaatttt gtgtcgccct tattcgactc actatagaag ttcctattct
    15121 ctagaaagta taggaacttc acttcatttt atttaaattt gcggccgcgc tgaggtctta
    15181 atgcggtagt ggggatgcct caattgtgct agctgcggcc gccttgctag atgactggta
    15241 ggaatctaac aaagggaaaa ccttgacgat tgggggcgga aaacatggca ttacaggggc
    15301 caatacggac gagctcatcg tttaatgaca ggcacaatgt atgcagcctt cctccacaac
    15361 actcgtactg gcactacact tttgctccaa gaggaaatca tgacttgccg catactctgg
    15421 ccatataagc ttcccagcct tctttctggt accgctgtgc agcttcaacc gggtcgggag
    15481 cagcgtagat gcctcgaccg gcgatgataa agtcggcacc gcgtccaata gccgatgcag
    15541 gagtctggta ttgctgtcca agcttatctc ctttggaaga gaggttcaca cccgtcgtga
    15601 agaccacgaa atcttcatcc tccgaggctg aagacacatc cgactgcact tccgtcaggg
    15661 cccgcgtcga cacgaaaccc ataacgaagt tcttgtattt gcgagcgtag tcaaccgatg
    15721 ccttggtata ctcgcccgta gccagcgatc ctttgggggt catctctgcc aggaccaaca
    15781 gtcctctctc aggaccatag gggaagtctt gcgcagatgc ggtctgggcc agagcctcga
    15841 cgatgccctc gccagggaga acgctgcagt tgataatgtg ggcccattcg gagatcctca
    15901 gagcaccgcc gtggtattgc ttctggacgg tattgccgat gtcgatgaat ttgcggtcct
    15961 cgaagatcaa aaagttgtac ttttgagcca gcacattcag gccattgata gtgtcgacgc
    16021 tgaaatcggt gaggatgtcg atgtgtgtct tgatgacggc gatgtaggga ccgagacctg
    16081 tatcatcaaa agttgattag tccattgcta gacggcatat gtattggatc caacagcttc
    16141 cgtacggtca gcgaggtcca ggagttctcg ggttgtcgtc acatcagcag agacggtaac
    16201 gtttgtcttc tttgcttcgg caatctcaaa aagtctcttt gccagaggat tggggtgctt
    16261 gctggctcga gcaccgtaag tcaattgcga cttggacgac atcgtgggaa tggagggtta
    16321 tatgcggggt atgacggtga ttgatgagtt gaagtagccg agcaatgagg tatattatcc
    16381 aattgagggt gacccaacca aatcaattgc ttgagaatca aatctcaaat attctaaaat
    16441 agaagccgcc ggggactaaa tttaaagtcg tactctatct cgatcttttc cgcgtgtaaa
    16501 aattgcgtgc caatctggat ggagacaggc cacatcggtg ctgtattcct ccgcctccga
    16561 ctgtggggtc aaccacaatt aaactactct gtagtcaggt acagctagaa tggggtagac
    16621 aggcagaact gtagaagata aaacattggt caatcactgg taactccacg tctagaggat
    16681 ccccggccgg
//
