LOCUS       sequence-503027-       16690 bp    DNA     circular SYN 16-MAR-2026
DEFINITION  CRISPR-Cas9 vector containing a 20 nt protospacer sequence to target
            the IS1 locus where the DIVERSIFY gene landing platform is 
            integrated in Aspergillus nidulans. pyrG marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16690)
  TITLE     Mortensen Lab plasmids
REFERENCE   2  (bases 1 to 16690)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 16, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pDIV069
FEATURES             Location/Qualifiers
     source          1..16690
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(511..544)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(1014..1030)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1129..1146)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1300..1888)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1380..1399)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2010..2801,2802..2870))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2633..2652
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2871..2962)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3004..3023
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     3029..3048
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     protein_bind    3255..3288
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     rep_origin      3601..8848
                     /label=AMA1
                     /note="replication origin from Aspergillus nidulans 
                     (Aleksenko and Clutterbuck, 1996)"
     misc_RNA        9681..9756
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             10958..15061
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             10958..15061
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             15065..15085
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 agtgcagtcg gatgtgtctt cagcctcgga ggatgaagat ttcgtggtct tcacgacggg
       61 tgtgaacctc tcttccaaag gagataagct tggacagcaa taccagactc ctgcatcggc
      121 tattggacgc ggtgccgact ttatcatcgc cggtcgaggc atctacgctg ctcccgaccc
      181 ggttgaagct gcacagcggt accagaaaga aggctgggaa gcttatatgg ccagagtatg
      241 cggcaagtca tgatttcctc ttggagcaaa agtgtagtgc cagtacgagt gttgtggagg
      301 aaggctgcat acattgtgcc tgtcattaaa cgatgagctc gtccgtattg gcccctgtaa
      361 tgccatgttt tccgccccca atcgtcaagg ttttcccttt gttagattcc taccagtcat
      421 ctagcaaggc ggccgcagct agcacaattg aggcatcccc actaccgcat taagacctca
      481 gcgcggccgc aaatttaaat aaaatgaagt gaagttccta tactttctag agaataggaa
      541 cttctatagt gagtcgaata agggcgacac aaaatttatt ctaaatgcat aataaatact
      601 gataacatct tatagtttgt attatatttt gtattatcgt tgacatgtat aattttgata
      661 tcaaaaactg attttccctt tattattttc gagatttatt ttcttaattc tctttaacaa
      721 actagaaata ttgtatatac aaaaaatcat aaataataga tgaatagttt aattataggt
      781 gttcatcaat cgaaaaagca acgtatctta tttaaagtgc gttgcttttt tctcatttat
      841 aaggttaaat aattctcata tatcaagcaa agtgacaggc gcccttaaat attctgacaa
      901 atgctctttc cctaaactcc ccccataaaa aaacccgccg aagcgggttt ttacgttatt
      961 tgcggattaa cgattactcg ttatcagaac cgcccagggg gcccgagctt aagactggcc
     1021 gtcgttttac aacacagaaa gagtttgtag aaacgcaaaa aggccatccg tcaggggcct
     1081 tctgcttagt ttgatgcctg gcagttccct actctcgcct tccgcttcct cgctcactga
     1141 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     1201 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     1261 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     1321 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     1381 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     1441 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     1501 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     1561 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     1621 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     1681 gtatgtaggc ggtgctacag agttcttgaa gtggtgggct aactacggct acactagaag
     1741 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     1801 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     1861 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     1921 cgctcagtgg aacgacgcgc gcgtaactca cgttaaggga ttttggtcat gagcttgcgc
     1981 cgtcccgtca agtcagcgta atgctctgct taccaatgct taatcagtga ggcacctatc
     2041 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     2101 acgatacggg agggcttacc atctggcccc agcgctgcga tgataccgcg agaaccacgc
     2161 tcaccggctc cggatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     2221 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     2281 agtagttcgc cagttaatag tttgcgcaac gttgttgcca tcgctacagg catcgtggtg
     2341 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     2401 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     2461 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcgctaca taattctctt
     2521 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     2581 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     2641 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     2701 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     2761 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     2821 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat attcttcctt
     2881 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     2941 tgtatttaga aaaataaaca aataggggtc agtgttacaa ccaattaacc aattctgaac
     3001 attatcgcga gcccatttat acctgaatat ggctcataac accccttgtt tgcctggcgg
     3061 cagtagcgcg gtggtcccac ctgaccccat gccgaactca gaagtgaaac gccgtagcgc
     3121 cgatggtagt gtggggactc cccatgcgag agtagggaac tgccaggcat caaataaaac
     3181 gaaaggctca gtcgaaagac tgggcctttc gcccgggcta attatggggt gtcgccctta
     3241 ttcgactcta tagtgaagtt cctattctct agaaagtata ggaacttctg aagtggggat
     3301 ttaaatgcgg ccgcgctgag ggtttaatcg acgaagcagc tgacggccag tgccaagctt
     3361 aacgcgtacc gggcccagta tatgttccgc agatgactgg agctctgcca tacgtgccct
     3421 ctcaagcacc atttgttcca tctacagaga ctagtcacca actagtctat caagactcac
     3481 agggtacatt gctgagacca actgaccaga ggcagggtag cggattgacg gctccatctc
     3541 cttcacttac aaggtctatt gaaagccctt tagcatcacc aagcggagaa tagattgtta
     3601 agcttatttt ttgtatactg ttttgtgata gcacgaagtt tttccacggt atcttgttaa
     3661 aaatatatat ttgtggcggg cttacctaca tcaaattaat aagagactaa ttataaacta
     3721 aacacacaag caagctactt tagggtaaaa gtttataaat gcttttgacg tataaacgtt
     3781 gcttgtattt attattacaa ttaaaggtgg atagaaaacc tagagactag ttagaaacta
     3841 atctcaggtt tgcgttaaac taaatcagag cccgagaggt taacagaacc tagaagggga
     3901 ctagatatcc gggtagggaa acaaaaaaaa aaacaagaca gccacatatt agggagacta
     3961 gttagaagct agttccagga ctaggaaaat aaaagacaat gataccacag tctagttgac
     4021 aactagatag attctagatt gaggccaaag tctctgagat ccaggttagt tgcaactaat
     4081 actagttagt atctagtctc ctataactct gaagctagaa taacttacta ctattatcct
     4141 caccactgtt cagctgcgca aacggagtga ttgcaaggtg ttcagagact agttattgac
     4201 tagtcagtga ctagcaataa ctaacaaggt attaacctac catgtctgcc atcaccctgc
     4261 acttcctcgg gctcagcagc cttttcctcc tcattttcat gctcattttc cttgtttaag
     4321 actgtgacta gtcaaagact agtccagaac cacaaaggag aaatgtctta ccactttctt
     4381 cattgcttgt ctcttttgca ttatccatgt ctgcaactag ttagagtcta gttagtgact
     4441 agtccgacga ggacttgctt gtctccggat tgttggagga actctccagg gcctcaagat
     4501 ccacaacaga gccttctaga agactggtca ataactagtt ggtctttgtc tgagtctgac
     4561 ttacgaggtt gcatactcgc tccctttgcc tcgtcaatcg atgagaaaaa gcgccaaaac
     4621 tcgcaatatg gctttgaacc acacggtgct gagactagtt agaatctagt cccaaactag
     4681 cttggatagc ttacctttgc cctttgcgtt gcgacaggtc ttgcagggta tggttccttt
     4741 ctcaccagct gatttagctg ccttgctacc ctcacggcgg atctgcataa agagtggcta
     4801 gaggttataa attagcactg atcctaggta cggggctgaa tgtaacttgc ctttcctttc
     4861 tcatcgcgcg gcaagacagg cttgctcaaa ttcctaccag tcacaggggt atgcacggcg
     4921 tacggaccac ttgaactagt cacagattag ttagcaacta gtctgcattg aatggctgta
     4981 cttacgggcc ctcgccattg tcctgatcat ttccagcttc accctcgttg ctgcaaagta
     5041 gttagtgact agtcaaggac tagttgaaat gggagaagaa actcacgaat tctcgacacc
     5101 cttagtattg tggtccttgg acttggtgct gctatatatt agctaataca ctagttagac
     5161 tcacagaaac ttacgcagct cgcttgcgct tcttggtagg agtcggggtt gggagaacag
     5221 tgccttcaaa caagccttca taccatgcta cttgactagt cagggactag tcaccaagta
     5281 atctagatag gacttgcctt tggcctccat cagttccttc atagtgggag gtccattgtg
     5341 caatgtaaac tccatgccgt gggagttctt gtccttcaag tgcttgacca atatgtttct
     5401 gttggcagag ggaacctgtc aactagttaa taactagtca gaaactagta tagcagtaga
     5461 ctcactgtac gcttgaggca tcccttcact cggcagtaga cttcatatgg atggatatca
     5521 ggcacgccat tgtcgtcctg tggactagtc agtaactagg cttaaagcta gtcgggtcgg
     5581 cttactatct tgaaatccgg cagcgtaagc tccccgtcct taactgcctc gagatagtga
     5641 cagtactctg gggactttcg gagatcgtta tcgcgaatgc tcggcatact aatcgttgac
     5701 tagtcttgga ctagtcccga gcaaaaagga ttggaggagg aggaggaagg tgagagtgag
     5761 acaaagagcg aaataagagc ttcaaaggct atctctaagc agtatgaagg ttaagtatct
     5821 agttcttgac tagatttaaa agagatttcg actagttatg tacctggagt ttggatatag
     5881 gaatgtgttg tggtaacgaa atgtaagggg gaggaaagaa aaagtcggtc aagaggtaac
     5941 tctaagtcgg ccattccttt ttgggaggcg ctaaccataa acggcatggt cgacttagag
     6001 ttagctcagg gaatttaggg agttatctgc gaccaccgag gaacggcgga atgccaaaga
     6061 atcccgatgg agctctagct ggcggttgac aaccccacct tttggcgttt ctgcggcgtt
     6121 gcaggcggga ctggatactt cgtagaacca gaaaggcaag gcagaacgcg ctcagcaaga
     6181 gtgttggaag tgatagcatg atgtgccttg ttaactaggt caaaatctgc agtatgcttg
     6241 atgttatcca aagtgtgaga gaggaaggtc caaacataca cgattgggag agggcctagg
     6301 tataagagtt tttgagtaga acgcatgtga gcccagccat ctcgaggaga ttaaacacgg
     6361 gccggcattt gatggctatg ttagtacccc aatggaaacg gtgagagtcc agtggtcgca
     6421 gataactccc taaattccct gagctaactc taagtcgacc atgccgttta tggttagcgc
     6481 ctcccaaaaa ggaatggccg acttagagtt acctcttgac cgactttttc tttcctcccc
     6541 cttacatttc gttaccacaa cacattccta tatccaaact ccaggtacat aactagtcga
     6601 aatctctttt aaatctagtc aagaactaga tacttaacct tcatactgct tagagatagc
     6661 ctttgaagct cttatttcgc tctttgtctc actctcacct tcctcctcct cctccaatcc
     6721 tttttgctcg ggactagtcc aagactagtc aacgattagt atgccgagca ttcgcgataa
     6781 cgatctccga aagtccccag agtactgtca ctatctcgag gcagttaagg acggggagct
     6841 tacgctgccg gatttcaaga tagtaagccg acccgactag ctttaagcct agttactgac
     6901 tagtccacag gacgacaatg gcgtgcctga tatccatcca tatgaagtct actgccgagt
     6961 gaagggatgc ctcaagcgta cagtgagtct actgctatac tagtttctga ctagttatta
     7021 actagttgac aggttccctc tgccaacaga aacatattgg tcaagcactt gaaggacaag
     7081 aactcccacg gcatggagtt tacattgcac aatggacctc ccactatgaa ggaactgatg
     7141 gaggccaaag gcaagtccta tctagattac ttggtgacta gtccctgact agtcaagtag
     7201 catggtatga aggcttgttt gaaggcactg ttctcccaac cccgactcct accaagaagc
     7261 gcaagcgagc tgcgtaagtt tccgtgagtc taactagtgt attagctaat atatagcagc
     7321 accaagtcca aggaccacaa tactaagggt gtcgagaatt cgtgagtttc ttctcccatt
     7381 tcaactagtc cttgactagt cactaactac tttgcagcaa cgagggtgaa gctggaaatg
     7441 atcaggacaa tggcgagggc ccgtaagtac agccattcaa tgcagactag ttgctaacta
     7501 atctgtgact agttcaagtg gtccgtacgc cgtgcatacc cctgtgactg gtaggaattt
     7561 gagcaagcct gtcttgccgc gcgatgagaa aggaagggca agttacattc agccccgtac
     7621 ctaggatcag tgctaattta taacctctag ccactcttta tgcagatccg ccgtgagggt
     7681 agcaaggcag ctaaatcagc tggtgagaaa ggaaccatac cctgcaagac ctgtcgcaac
     7741 gcaaagggca aaggtaagct atccaagcta gtttgggact agattctaac tagtctcagc
     7801 accgtgtggt tcaaagccat attgcgagtt ttggcgcttt ttctcatcga ttgacgaggc
     7861 aaagggagcg agtatgcaac ctcgtaagtc agactcagac aaagaccaac tagttattga
     7921 ccagtcttct agaaggctct gttgtggatc ttgaggccct ggagagttcc tccaacaatc
     7981 cggagacaag caagtcctcg tcggactagt cactaactag actctaacta gttgcagaca
     8041 tggataatgc aaaagagaca agcaatgaag aaagtggtaa gacatttctc ctttgtggtt
     8101 ctggactagt cttcgactag tcacagtctt aaacaaggaa aatgagcatg aaaatgagga
     8161 ggaaaaggct gctgagcccg aggaagtgca gggtgatggc agacatggta ggttaatacc
     8221 ttgttagtta ttgctagtca ctgactagtc aataactagt ctctgaacac cttgcaatca
     8281 ctccgtttgc gcagctgaac agtggtgagg ataatagtag taagttattc tagcttcaga
     8341 gttataggag actagatact aactagtatt agttgcaact aacctggatc tcagagactt
     8401 tggcctcaat ctagaatcta tctagttgtc aactagactg tggtatcatt gtcttttatt
     8461 ttcctagtcc tggaactagc ttctaactag tctccctaat atgtggctgt cttgtttttt
     8521 ttttttgttt ccctacccgg atatctagtc cccttctagg ttctgttaac ctctcgggct
     8581 ctgatttagt ttaacgcaaa cctgagatta gtttctaact agtctctagg ttttctatcc
     8641 acctttaatt gtaataataa atacaagcaa cgtttatacg tcaaaagcat ttataaactt
     8701 ttaccctaaa gtagcttgct tgtgtgttta gtttataatt agtctcttat taatttgatg
     8761 taggtaagcc cgccacaaat atatattttt aacaagatac cgtggaaaaa cttcgtgcta
     8821 tcacaaaaca gtatacaaaa aataagctta acaatctatt ctccgcttgg tgatgctaaa
     8881 gggctttcaa tagaccttgt aagtgaagga gatggagccg tcaatccgct accctgcctc
     8941 tggtcagttg gtctcagcaa tgtaccctgt gagtcttgat agactagttg gtgactagtc
     9001 tctgtagatg gaacaaatgg tgcttgagag ggcacgtatg gcagagctcc agtcatctgc
     9061 ggaacatata ctgggcccgg gaagatctca tggtcatagc tgtttccgct gagggtttaa
     9121 tgatcacata gatgctcggt tgacaggacc acctatagca agtactttgt aggaggttct
     9181 cctaacgctt ggcttcaaat ccaatcagga tatcagtgag ttactgtttg tcatcgcatc
     9241 ctcatgaagt cgctcaacag ccactgagaa gcaaatattt gggagaccat ccctgatgtt
     9301 gaaattttac gctggagccc attcaccggt gagctcgggg acagtctggc cagtggagcg
     9361 gaaaatcttc aactaactct gattggtacc acaggtagcc ccacagaaca aaccgagcaa
     9421 acatgaaaat tttcgcttga ggttagcgca ctcgctagcg cctgcccgca aatataaaag
     9481 gccccgaaat cccatgcatt ttggaaatca agcgactcta cgtatgtcat ccccctccct
     9541 acctttggga aaacctctca actgcaagtc agaacatttt gctaacagcg catcattggt
     9601 ctagtggtag aattcatcgt tgccatcgat gaggcccgtg ttcgattcac ggatgatgca
     9661 tgattgctgg tctgtgtaag gttttagagc tagaaatagc aagttaaaat aaggctagtc
     9721 cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt gcatcattgg tctagtggta
     9781 gaattcatcg ttgccatcga tgaggcccgt gttcgattca cggatgatgc atgtcaattt
     9841 ttacacttga tgttggtgta atcaggcgat cagagtttgc tcaaattctc tcttgtttta
     9901 gattctgaag aatataattt ttacatgact agttcatttt acttccaagt agactatttg
     9961 tagattgaga ttgccgtcaa ctttattcat agaatcacat taaattgctc attgacctgc
    10021 atgtctatgc acacattcac atctatcttc tcagggtatt aagacctcag ccgagacagc
    10081 agaatcaccg cccaagttaa gcctttgtgc tgatcatgct ctcgaacggg ccaagttcgg
    10141 gaaaagcaaa ggagcgttta gtgaggggca atttgactca cctcccaggc aacagatgag
    10201 gggggcaaaa agaaagaaat tttcgtgagt caatatggat tccgagcatc attttcttgc
    10261 ggtctatctt gctacgtatg ttgatcttga cgctgtggat caagcaacgc cactcgctcg
    10321 ctccatcgca ggctggtcgc agacaaatta aaaggcggca aactcgtaca gccgcggggt
    10381 tgtccgctgc aaagtacaga gtgataaaag ccgccatgcg accatcaacg cgttgatgcc
    10441 cagctttttc gatccgagaa tccaccgtag aggcgatagc aagtaaagaa aagctaaaca
    10501 aaaaaaaatt tctgccccta agccatgaaa acgagatggg gtggagcaga accaaggaaa
    10561 gagtcgcgct gggctgccgt tccggaaggt gttgtaaagg ctcgacgccc aaggtgggag
    10621 tctaggagaa gaatttgcat cgggagtggg gcgggttacc cctccatatc caatgacaga
    10681 tatctaccag ccaagggttt gagcccgccc gcttagtcgt cgtcctcgct tgcccctcca
    10741 taaaaggatt tcccctcccc ctcccacaaa attttctttc ccttcctctc cttgtccgct
    10801 tcagtacgta tatcttccct tccctcgctt ctctcctcca tccttctttc atccatctcc
    10861 tgctaacttc tctgctcagc acctctacgc attactagcc gtagtatctg agcacttctc
    10921 ccttttatat tccacaaaac ataacacaac cttcaccatg gacaagaagt atagcatcgg
    10981 gctggacatt ggaacgaact cggttggttg ggctgtgatt acggacgaat acaaggtgcc
    11041 atccaagaag tttaaggtcc tgggaaacac cgaccgtcac tcaatcaaga agaatctcat
    11101 tggagccctg ctcttcgata gtggggagac cgccgaagct actcgactga agcgaacggc
    11161 tcgccggcgt tatacacgac gcaagaatcg catctgctac ctccaggaga ttttcagcaa
    11221 cgaaatggct aaggttgatg actcattctt tcatcgactc gaagaaagtt tcttggtcga
    11281 ggaggataag aagcacgagc gccatccgat ctttggtaac attgtggatg aggttgccta
    11341 tcacgaaaag tacccaacta tctatcatct tcgtaagaag ctggtcgata gcacggacaa
    11401 ggctgatttg cgacttatct acctggcact cgcgcacatg attaagttcc gcggccattt
    11461 tcttatcgag ggtgacctga accccgataa ttctgacgtt gataagctct tcatccagtt
    11521 ggtccaaacc tacaatcagc tgtttgagga aaaccctatt aatgcatctg gcgtggacgc
    11581 caaggctatc ctttcggcgc gcctgtctaa gtcgcggcgt ttggagaacc ttatcgcaca
    11641 actccccggc gaaaagaaga acggcctctt cggtaatttg attgcgttgt cacttggtct
    11701 gactcctaac ttcaagagta attttgacct ggcagaggat gcgaagctcc agttgtctaa
    11761 ggatacgtat gatgacgatc tcgacaactt gcttgcccaa atcggtgacc agtacgctga
    11821 tcttttcctg gccgctaaga atctctcaga tgcaatcctg ctcagtgaca ttttgcgggt
    11881 caacaccgag attactaagg cccccctgtc agctagtatg atcaagcggt atgatgagca
    11941 ccatcaggac ctcaccttgc ttaaggccct cgtgcgtcag caattgcctg agaagtataa
    12001 ggaaatcttc tttgaccaat ccaagaacgg atacgcaggg tatattgatg gcggtgcgag
    12061 ccaggaggaa ttctacaagt ttatcaagcc gattttggag aagatggacg gcactgagga
    12121 actgctcgtc aagctgaatc gcgaagattt gcttcgtaag caacgaacgt tcgacaacgg
    12181 ctccatcccg caccagattc atctgggcga gctccacgcc atccttcgac gccaggaaga
    12241 tttctaccca tttctgaagg acaaccgtga gaagatcgaa aagattctta cattccgaat
    12301 cccctactat gtgggacctt tggcccgtgg gaattcccga tttgcttgga tgacccgaaa
    12361 gagcgaggaa accatcactc cgtggaactt cgaggaagtc gtggacaagg gtgcatccgc
    12421 gcagagcttc attgagcgga tgaccaattt tgataagaac cttccgaatg aaaaggtcct
    12481 gccaaagcat tcgctgctct acgagtattt caccgtgtat aacgaactga ctaaggtcaa
    12541 gtacgtgacg gagggaatgc ggaagccagc cttcctctca ggggaacaaa agaaggctat
    12601 cgtcgatttg ctttttaaga ccaatcgtaa agtgactgtt aagcagctga aggaggatta
    12661 tttcaagaag attgaatgtt tcgactccgt cgagatcagc ggcgtggaag atcgctttaa
    12721 cgcttccctc ggtacctacc acgacctgct caagatcatt aaggacaagg atttcctcga
    12781 taacgaggaa aatgaggaca tcttggaaga tattgtcctc acgttgacac tttttgagga
    12841 ccgcgaaatg atcgaggaac ggctcaagac atatgcccat ttgttcgacg ataaggtgat
    12901 gaagcagctg aagcggcgtc gatacaccgg atggggtcgc cttagccgga agctgatcaa
    12961 cggcattcga gataagcaat ctggtaagac tatcttggat ttccttaagt cggacggctt
    13021 cgccaaccgc aattttatgc agcttattca cgacgattcc ctgacgttca aggaggacat
    13081 ccagaaggca caagtctcag gacaagggga ttccctgcac gagcatatcg ccaacctggc
    13141 tggatccccg gcgatcaaga aggggattct tcagaccgtc aaggttgtcg acgagctggt
    13201 caaggtgatg ggccgtcata agccagaaaa catcgtgatt gagatggccc gagaaaatca
    13261 gaccactcaa aagggtcaga agaacagccg cgagcggatg aagcggatcg aggaaggcat
    13321 taaggaactt ggttctcaga tcctgaagga gcaccctgtt gaaaacacac agctccaaaa
    13381 tgagaagctg tatctctact atttgcaaaa tggacgcgac atgtacgtcg atcaggagct
    13441 cgacattaac cggttgtcgg actacgatgt tgaccatatc gtcccgcaat ccttccttaa
    13501 ggacgatagc attgataaca aggtgctgac tcgctcagat aagaaccggg gcaagtccga
    13561 caatgttcca agcgaggaag tggttaagaa gatgaagaac tactggcgcc aattgcttaa
    13621 tgccaagctc atcacacagc gcaagtttga caacttgacc aaggccgagc ggggagggct
    13681 gagtgaactc gataaggctg gcttcatcaa gcgtcaactc gtggagacgc gacagatcac
    13741 aaagcacgtt gctcagattc tggactcccg gatgaacaca aagtacgacg agaatgataa
    13801 gctcatccgt gaagttaagg tcattaccct caagtctaag ttggtgtcgg atttccgcaa
    13861 ggacttccaa ttttataagg ttcgggagat caacaattat caccatgcac atgatgcgta
    13921 cctcaacgca gtcgtgggaa ctgcgctcat caagaagtat cccaagttgg agtccgaatt
    13981 cgtctacggg gattataagg tttacgacgt ccgcaagatg atcgccaaga gtgagcagga
    14041 aattggcaag gccacggcta agtatttctt ttactccaac atcatgaatt tctttaagac
    14101 ggagatcaca ctcgccaatg gagaaatccg taagcgacct ttgattgaga ccaacggcga
    14161 gactggtgaa atcgtttggg ataaggggcg cgacttcgct accgtgcgga aggttctgag
    14221 catgccgcaa gtcaatatcg tcaagaaaac cgaggtgcag acaggcggtt tctctaagga
    14281 atcgattctt ccaaagcgta actctgacaa gctgatcgct cgaaagaagg attgggaccc
    14341 caagaagtat ggagggttcg attctcctac agtggcatac tcggttctcg ttgtcgcgaa
    14401 ggttgagaag ggaaagtcta agaagctgaa gtcggtcaag gaactgctcg ggatcaccat
    14461 tatggagcgc tccagcttcg aaaagaatcc catcgacttt ctcgaggcca agggctataa
    14521 ggaagtcaag aaggatctta tcattaagct gcctaagtac tctttgttcg agcttgaaaa
    14581 cggtcgaaag cgaatgctcg catcggcagg agagttgcag aaggggaatg aattggcact
    14641 tccctcaaag tacgtgaact tcctgtatct cgcgtcccac tacgagaagc tgaagggtag
    14701 ccctgaggac aacgaacaga agcaactttt tgttgagcaa cacaagcatt atctggatga
    14761 gatcattgaa cagatttcag agttcagtaa gcgcgtcatc ctcgccgatg ctaatctcga
    14821 caaggtgttg tcggcctaca acaagcaccg tgacaagccg atccgagagc aggctgaaaa
    14881 tatcattcat ctgttcaccc tcactaactt gggagcacca gcagcgttca agtattttga
    14941 tacgacaatc gaccgtaagc gatacacgtc cacaaaggag gtgcttgatg cgaccctgat
    15001 tcatcaatcc atcactgggc tctatgaaac ccgtatcgac cttagtcaac tggggggcga
    15061 ccctcccaag aagaagcgca aggtctgagc ggacattcga tttatgccgt tatgacttcc
    15121 ttaaaaaagc ctttacgaat gaaagaaatg gaattagact tgttatgtag ttgattctac
    15181 aatggattat gattcctgaa cttcaaatcc gctgttcatt attaatctca gctcttcccg
    15241 taaagccaat gttgaaacta ttcgtaaatg tacctcgttt tgcgtgtacc ttgcttatca
    15301 cgtgatatta catgacctgg acagagttct gcgcgaaagt cataacgtaa atcccgggcg
    15361 gtaggtgcgt cccgggcgga aggtagtttt ctcgtccacc ccaacgcgtt tatcaacctc
    15421 aactttcaac aaccatcatg ccaccaaaag cgcgtaaaac aaagcgagat ttgattgagc
    15481 aagagggcag gatccaatgc gcgattcaag acattaaaaa tggaaaattt caaaaaattg
    15541 cgcccgcagc gcgtgcatac aaaattcatc ccaatacaag tgtaattcct cgtgtactgt
    15601 gtaagcgccc actaggtaat atgacatgat tacgaattcg agctcggtac ccggccgggg
    15661 atcctctaga cgtggagtta ccagtgattg accaatgttt tatcttctac agttctgcct
    15721 gtctacccca ttctagctgt acctgactac agagtagttt aattgtggtt gaccccacag
    15781 tcggaggcgg aggaatacag caccgatgtg gcctgtctcc atccagattg gcacgcaatt
    15841 tttacacgcg gaaaagatcg agatagagta cgactttaaa tttagtcccc ggcggcttct
    15901 attttagaat atttgagatt tgattctcaa gcaattgatt tggttgggtc accctcaatt
    15961 ggataatata cctcattgct cggctacttc aactcatcaa tcaccgtcat accccgcata
    16021 taaccctcca ttcccacgat gtcgtccaag tcgcaattga cttacggtgc tcgagccagc
    16081 aagcacccca atcctctggc aaagagactt tttgagattg ccgaagcaaa gaagacaaac
    16141 gttaccgtct ctgctgatgt gacgacaacc cgagaactcc tggacctcgc tgaccgtacg
    16201 gaagctgttg gatccaatac atatgccgtc tagcaatgga ctaatcaact tttgatgata
    16261 caggtctcgg tccctacatc gccgtcatca agacacacat cgacatcctc accgatttca
    16321 gcgtcgacac tatcaatggc ctgaatgtgc tggctcaaaa gtacaacttt ttgatcttcg
    16381 aggaccgcaa attcatcgac atcggcaata ccgtccagaa gcaataccac ggcggtgctc
    16441 tgaggatctc cgaatgggcc cacattatca actgcagcgt tctccctggc gagggcatcg
    16501 tcgaggctct ggcccagacc gcatctgcgc aagacttccc ctatggtcct gagagaggac
    16561 tgttggtcct ggcagagatg acccccaaag gatcgctggc tacgggcgag tataccaagg
    16621 catcggttga ctacgctcgc aaatacaaga acttcgttat gggtttcgtg tcgacgcggg
    16681 ccctgacgga
//
