LOCUS       sequence-503056-        9142 bp    DNA     circular SYN 16-MAR-2026
DEFINITION  PBT0779+20xTetO biosensor cicuit in pNBU2 integrative Bacteroides 
            plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9142)
  TITLE     Tropini Lab plasmids
REFERENCE   2  (bases 1 to 9142)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 16, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pJB177
FEATURES             Location/Qualifiers
     source          1..9142
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             207..893
                     /codon_start=1
                     /product="bright cysteine-free monomeric red fluorescent 
                     protein with fast and complete maturation (Gadella et al., 
                     2023)"
                     /label=mScarlet3
                     /translation="MDSTEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYWKQSFPEGFKWERVMIFEDGGT
                     VSVTQDTSLEDGTLIYKVKLRGGNFPPDGPVMQKRTMGWEASTERLYPEDVVLKGDIKM
                     ALRLKDGGRYLADFKTTYKAKKPVQMPGAFNIDRKLDITSHNEDYTVVEQYERSVARHS
                     TGGSGGS"
     primer_bind     complement(337..355)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     terminator      919..946
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     protein_bind    1023..1041
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1070..1088
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1119..1137
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1151..1169
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1193..1211
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1239..1257
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1281..1299
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1329..1347
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1378..1396
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1420..1438
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1483..1501
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1530..1548
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1579..1597
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1611..1629
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1653..1671
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1699..1717
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1741..1759
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1789..1807
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1838..1856
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1880..1898
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      1933..2004
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2020..2047
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             2545..3168
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(2618..2637)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     protein_bind    3333..3351
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             join(3438..3440,3441..3443,3444..4157)
                     /codon_start=1
                     /product="monomeric GFP derivative that displays 
                     exceptionally high brightness in cells (Campbell et al., 
                     2020)"
                     /label=mGreenLantern
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLGYGVACFARYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIVLKGIDFKEDGNILGHKLEYNFNSHKVYITADKQKNGIK
                     ANFKTRHNVEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLK
                     ERVTAAGITHDMDELYK"
     primer_bind     complement(3483..3504)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     terminator      4178..4205
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(join(6185..6976,6977..7045))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6808..6827
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8337..8725
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     oriT            8896..9005
                     /note="incP origin of transfer"
ORIGIN
        1 caattgggct accttttttt tgttttgttt gcaatggtta atctattgtt aaaatttaaa
       61 gtttcacttg aactttcaaa taatgttctt atatttgcag tgtcgaaaga aacaaagtag
      121 gactgatcac acaacattta aaaaataaca ttatgaaagc actcgaaaag agattcaaag
      181 gtaatattaa caataattta ttttcaatgg atagcaccga ggcagtgatc aaggagttca
      241 tgcggttcaa ggtgcacatg gagggctcca tgaacggcca cgagttcgag atcgagggcg
      301 agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg
      361 gccccctgcc cttctcctgg gacatcctgt cccctcagtt catgtacggc tccagggcct
      421 tcatcaagca ccccgccgac atccccgact actggaagca gtccttcccc gagggcttca
      481 agtgggagcg cgtgatgatc ttcgaggacg gcggcaccgt gtccgtgacc caggacacct
      541 ccctggagga cggcaccctg atctacaagg tgaagctccg cggcggcaac ttccctcctg
      601 acggccccgt aatgcagaag aggacaatgg gctgggaagc atccaccgag cggttgtacc
      661 ccgaggacgt cgtgctgaag ggcgacatta agatggccct gcgcctgaag gacggcggcc
      721 gctacctggc ggacttcaag accacctaca aggccaagaa gcccgtgcag atgcccggcg
      781 ccttcaacat cgaccgcaag ttggacatca cctcccacaa cgaggactac accgtggtgg
      841 aacagtacga acgctccgtg gcccgccact ccaccggcgg ctccggtggc tcctaataaa
      901 tcctagctga ttagctcgag aaggccatcc tgacggatgg cctttttttt gtatggggac
      961 tctgagaacc ctacctttca aaccccgagc ggccgcttct agagatcgat tttagagctg
     1021 cctccctatc agtgatagag attgctacaa ctgcaaggta gtcccggtat ccctatcagt
     1081 gatagagact gatagctacg agtctgagat cctacttgtc cctatcagtg atagagacag
     1141 taccgagttt tccctatcag tgatagagag ctcatactgc gacagagtct tttccctatc
     1201 agtgatagag ataggctcct ttgtcaaaag tgatcgcttc cctatcagtg atagagactt
     1261 cacgggataa tgtgttctac tccctatcag tgatagagag cttaagtcaa tccaatgcgt
     1321 tcaggtgctc cctatcagtg atagagaaac tccgtcatcg tggatgtcaa tactgactcc
     1381 ctatcagtga tagagattga tttcaccgct ggcgtcaatt ccctatcagt gatagagact
     1441 taggtgtccc tagaattact agagatcgat tttagagctg cctccctatc agtgatagag
     1501 attgctacaa ctgcaaggta gtcccggtat ccctatcagt gatagagact gatagctacg
     1561 agtctgagat cctacttgtc cctatcagtg atagagacag taccgagttt tccctatcag
     1621 tgatagagag ctcatactgc gacagagtct tttccctatc agtgatagag ataggctcct
     1681 ttgtcaaaag tgatcgcttc cctatcagtg atagagactt cacgggataa tgtgttctac
     1741 tccctatcag tgatagagag cttaagtcaa tccaatgcgt tcaggtgctc cctatcagtg
     1801 atagagaaac tccgtcatcg tggatgtcaa tactgactcc ctatcagtga tagagattga
     1861 tttcaccgct ggcgtcaatt ccctatcagt gatagagact taggtgtccc tagaattact
     1921 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
     1981 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct
     2041 ttctgcgttt atatatgatg gcagcatcca ttgcctgaaa aataaccaga accgagtagc
     2101 ggtcttgtgc ataaagcata ctttgcagtt ctgacagctt ttcgatattt tgggctaact
     2161 gttctttggc atcctgtttg gacatatcag cagtaaatga gggattgaaa tcggatacat
     2221 gatgttcctt ccccggtttg gctgtaagct cttttaagac ttcttttttc attgttgtat
     2281 aattaggttg ttataggtat ataacagttg aataaagaaa tcagttcttg ccaaattaag
     2341 agcgttgaat agtaattccc caattatgta tcaacagatt ctacataatt ccacactaat
     2401 tccacaatta tatttatatt gtctgctgtt tatatgtttg attatcagtt atatatggcg
     2461 gagtgaaacg tttggcatct gctttgctat atattaagta caagaaacaa tagttctatt
     2521 attcacactt aaaaaattac gattatgtcc agattagata aaagtaaagt gattaacagc
     2581 gcattagagc tgcttaatga ggtcggaatc gaaggtttaa caacccgtaa actcgcccag
     2641 aagctaggtg tagagcagcc tacattgtat tggcatgtaa aaaataagcg ggctttgctc
     2701 gacgccttag ccattgagat gttagatagg caccatactc acttttgccc tttagaaggg
     2761 gaaagctggc aagatttttt acgtaataac gctaaaagtt ttagatgtgc tttactaagt
     2821 catcgcgatg gagcaaaagt acatttaggt acacggccta cagaaaaaca gtatgaaact
     2881 ctcgaaaatc aattagcctt tttatgccaa caaggttttt cactagagaa tgcattatat
     2941 gcactcagcg ctgtggggca ttttacttta ggttgcgtat tggaagatca agagcatcaa
     3001 gtcgctaaag aagaaaggga aacacctact actgatagta tgccgccatt attacgacaa
     3061 gctatcgaat tatttgatca ccaaggtgca gagccagcct tcttattcgg ccttgaattg
     3121 atcatatgcg gattagaaaa acaacttaaa tgtgaaagtg ggtcttaatg gccacatcgg
     3181 ttcctgctag aaggcccatc ctgacggatg gccttttggt attcgagcat tacctcatac
     3241 tttggatttt tgttttgttt gcaatggtta atctattgtt aaaatttaaa gtttcacttg
     3301 aactttcaaa taatgttctt atatttgcag tgtccctatc agtgatagag agtaggatca
     3361 cacaacattt aaaaaataac attatgaaag cactcgaaaa gagattcaaa ggtaatatta
     3421 acaataattt attttcaatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
     3481 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtccgc ggcgagggcg
     3541 agggcgatgc caccaacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
     3601 ccgtgccctg gcccaccctc gtgaccacct taggctacgg cgtggcctgc ttcgcccgct
     3661 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
     3721 aggagcgcac catctctttc aaggacgacg gtacctacaa gacccgcgcc gaggtgaagt
     3781 tcgagggcga caccctggtg aaccgcatcg tgctgaaggg catcgacttc aaggaggacg
     3841 gcaacatcct ggggcacaag ctggagtaca acttcaacag ccacaaggtc tatatcacgg
     3901 ccgacaagca gaagaacggc atcaaggcta acttcaagac ccgccacaac gttgaggacg
     3961 gcggcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
     4021 tgctgcccga caaccactac ctgagccatc agtccaagct gagcaaagac cccaacgaga
     4081 agcgcgatca catggtcctg aaggagaggg tgaccgccgc cgggattaca catgacatgg
     4141 acgagctgta caagtaatag cgttcagctc gactgtgaga aggccatcct gacggatggc
     4201 cttttttttt cgttataact agttgcgttc ctagccgcta ctagtgtaat tgcctatctt
     4261 ccagtgatgg aacagcattt gtgcattggc tgcaacaatc agccttactt gtgcctgttc
     4321 tatttccgaa ccgaccgctt gtatgaatgc atcaaaattc gttttctcta cgttggattc
     4381 cttgttgctc atattgtgat gataatttct acaaatatag tcattggtaa ctatctatga
     4441 aactgtttga tacttttata gttgattaaa cttgttcatg gcatttgcct taatatcatc
     4501 cgctatgtca atgtagggtt tcatagcttt gtagtcgctg tgtcccgtcc atttcatgac
     4561 cacctgtgcc gggattccga gagccagcgc attgcagatg aatgtccttt ttcctgcatg
     4621 ggtactgagc aaagcgtatt tgtgtgtgac ttcatcaata cgttcatttc ccttgtagta
     4681 ggtttcccgt acaggctcgt tgatttctgc cagttcgccc agctctttca ggtaatcgtt
     4741 catcttctgg ttgctgatga cgggcagagc catgtaattc tcgaaatgga tgtccttgta
     4801 tttgtccagt atggctttgc tgtatttgtt cagttcaatc gtcaggctgt cggcagtctt
     4861 gactgtggtt atttcgatgt ggtcggactt cacatcgctt cttttcagat tgcgaacatc
     4921 cgaataccgc aaactcgtaa agcagcagaa caggaaaaca tcacgcacac gttccaggta
     4981 ttgcttatcc ttgggtatct ggtagtcttt cagcttgttc agttcatccc aagtcaggaa
     5041 gattactttt ttcgaggtgg ttttcagttt cggtttgaac gtatcgtatg caatgttctg
     5101 atgatgtcct ttcttgaagc tccagcgcag gaaccatttg aggaatccca tttgcttgcc
     5161 gatggtgctg tttctcatat ccttggtgtc acgcaggaag ttgacgtatt cgttcaatcc
     5221 aaactcgttg aaatagttga acgttgcatc ctccttgaac tctttgaggt ggttcctcac
     5281 tgctgcaaat ttttcatagg tggatgccgt ccagttattc tggttaccgc actcttttac
     5341 aaactcatcg aacacctccc aaaagctgac aggggcttct tccggctgtt cttcactggt
     5401 gtctttcatt ctcatgttga aagcttcctt caactgttgg gtcgttggca tgacctcctg
     5461 cacctcaaat tccttgaaaa tattctggat ttcggcatag tatttcagca agtccgtatt
     5521 gatttcggct gcactttgct ttagcttgtt ggtacatccg ttctttaccc gctgcttatc
     5581 tgcatcccat ttggctacgt caatccggta gcccgttgta aactcgatac gttggctggc
     5641 aaagatgaca cgcatacgga tgggtacgtt ctctacgatt ggcacaccgt tctttttccg
     5701 gctctccaat gcaaaaatga tgttgcgctt gatattcata attgggtgcg tttgaaattc
     5761 tacacccaaa tatacaccca attattgaga tagcaaaaga catttagaaa catttacttt
     5821 tactctatat tgtaatttac acttgattat cagtcgtttg cagttttatg atattctgtg
     5881 aaagtataag ttcgagagcc tgtctctccg caaaaaacgc tgaaaatcag cagattgcaa
     5941 aacaaacacc ctgttttaca cccaagaatg taaagtcggg tgtttttgtt ttatttaaga
     6001 taatacaacc actacataat aaaagagtag cgatattaaa agaatccgat gagaaaagac
     6061 taatatttat ctatccattc agtttgattt ctcaggactt tacatcgtcc tgaaagtatt
     6121 tgttggtacc ggtaccgagg acgcgacagc tgcagacgcg ttatgagtaa acttggtctg
     6181 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     6241 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     6301 gccccagtgc tgcaatgata ccgcgggacc cacgctcacc ggctccagat ttatcagcaa
     6361 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     6421 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     6481 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     6541 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     6601 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     6661 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     6721 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     6781 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     6841 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     6901 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     6961 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     7021 cgacacggaa atgttgaata ctcatactct tcctttttca atcatggccg cgggattaaa
     7081 agtcggggat tggtgaacaa aaaggtgttt ctctctttaa gagaaatatc gttttgctaa
     7141 acagttgata ttgaggtatc attttatcgt aaaagacatt tttgctcaac aattgcttga
     7201 cggaaatcaa caaattttag cattttgtaa aaaagtcgct atataatttg gtgaattgga
     7261 gttattttca tatttttgca tcccgaagag tttctcttaa agagagaaac atcttttgca
     7321 taccttttcc gaccgaattt ttatgtcgta aagaggggct ttgcaggggg tggactcaga
     7381 aagatgagaa tagatgacta ttgtagttga aacacataga aagttgctga tatacagacc
     7441 gatacgcata tcgggatgaa ccatgagtac gttcttttct caaaaaacat aaatattcga
     7501 aaagagatgc aataaattaa ggagaggtta taatgaacaa agtaaatata aaagatagtc
     7561 aaaattttat tacttcaaaa tatcacatag aaaaaataat gaattgcata agtttagatg
     7621 aaaaagataa catctttgaa ataggtgcag ggaaaggtca ttttactgct ggattggtaa
     7681 agagatgtaa ttttgtaacg gcgatagaaa ttgattctaa attatgtgag gtaactcgta
     7741 ataagctctt aaattatcct aactatcaaa tagtaaatga tgatatactg aaatttacat
     7801 ttcctagcca caatccatat aaaatatttg gcaacatacc ttacaacata agcacaaata
     7861 taattcgaaa aattgttttt gaaagttcag ccacaataag ttatttaata gtggaatatg
     7921 gttttgctaa aatgttatta gatacaaaca gatcactagc attgctgtta atggcagagg
     7981 tagatatttc tatattagca aaaattccta ggtattattt ccatccaaaa cctaaagtgg
     8041 atagcacatt aattgtatta aaaagaaagc cagcaaaaat ggcatttaaa gagagaaaaa
     8101 aatatgaaac ttttgtaatg aaatgggtta acaaagagta cgaaaaactg tttacaaaaa
     8161 atcaatttaa taaagcttta aaacatgcga gaatatatga tataaacaat attagtttcg
     8221 aacaatttgt atcgctattt aatagttata aaatatttaa cggctaaaaa caataggcca
     8281 catgcaactg taaatgttta cgcgggtacc gacaccgcgg tggaggggaa ttcccatgtc
     8341 agccgttaag tgttcctgtg tcactcaaaa ttgctttgag aggctctaag ggcttctcag
     8401 tgcgttacat ccctggcttg ttgtccacaa ccgttaaacc ttaaaagctt taaaagcctt
     8461 atatattctt ttttttctta taaaacttaa aaccttagag gctatttaag ttgctgattt
     8521 atattaattt tattgttcaa acatgagagc ttagtacgtg aaacatgaga gcttagtacg
     8581 ttagccatga gagcttagta cgttagccat gagggtttag ttcgttaaac atgagagctt
     8641 agtacgttaa acatgagagc ttagtacgtg aaacatgaga gcttagtacg tactatcaac
     8701 aggttgaact gctgatcttc agatcctcta cgccggacgc atcgtggccg gatcaattcc
     8761 gttttccgct gcataaccct gcttcggggt cattatagcg attttttcgg tatatccatc
     8821 ctttttcgca cgatatacag gattttgcca aagggttcgt gtagactttc cttggtgtat
     8881 ccaacggcgt cagccgggca ggataggtga agtaggccca cccgcgagcg ggtgttcctt
     8941 cttcactgtc ccttattcgc acctggcggt gctcaacggg aatcctgctc tgcgaggctg
     9001 gccggctacc gccggcgtaa cagatgaggg caagcggatg gctgatgaaa ccaagccaac
     9061 caggaagggc agcccaccta tcacggaatt gatccccctc gaattggaat tctagcctgg
     9121 aatagggaag caccacatca cc
//
